公开(公告)号：US20080038256A1

公开(公告)日：2008-02-14

申请号：US11626055

申请日：2007-01-23

申请人： Junho CHUNG ,  Kisu Kim

发明人： Junho CHUNG ,  Kisu Kim

IPC分类号： A61K39/395 ,  A61P35/00 ,  C07H21/04 ,  C07K16/22 ,  C40B10/00

CPC分类号： C07K16/32 ,  A61K39/39558 ,  A61K2039/505 ,  C07K2317/24 ,  A61K2300/00

摘要： The inventive anti-HGF/SF humanized antibody prepared by displaying on the surface of a phage an anti-HGF/SF chimeric Fab library comprising a set of human antibody light chain variable region (VL) and human antibody heavy chain variable region (VH) which are grafted with heavy chain complementary determining regions (HCDRs) of an anti-HGF/SF antibody of an animal other than human, has the equal or greater binding affinity than that of the parent anti-HGF/SF antibody, the neutralizing activity inhibiting the binding of HGF/SF to its receptor, cMET, while having the reduced immunogenicity in human. Therefore, the inventive anti-HGF/SF humanized antibody can be used for preventing and treating diseases effectively, e.g., cancers, by the action of binding HGF/SF to cMET.

摘要翻译： 通过在噬菌体表面上显示包含一组人抗体轻链可变区（V L L L）和人的抗HGF / SF嵌合Fab文库制备的本发明抗HGF / SF人源化抗体 用除人以外的动物的抗HGF / SF抗体的重链互补决定区（HCDR）接枝的抗体重链可变区（V H H H S）具有相等或更大的结合亲和力 与抗原HGF / SF抗体相比，中和活性抑制HGF / SF与其受体cMET的结合，同时在人体中具有降低的免疫原性。 因此，通过将HGF / SF结合到cMET的作用，本发明的抗HGF / SF人源化抗体可用于有效地预防和治疗疾病，例如癌症。

公开(公告)号：US06962807B2

公开(公告)日：2005-11-08

申请号：US10189505

申请日：2002-07-08

申请人： Philippe Marliere ,  Rupert Mutzel ,  Didier Mazel

发明人： Philippe Marliere ,  Rupert Mutzel ,  Didier Mazel

IPC分类号： C12N1/21 ,  C12N15/09 ,  C12R1/19 ,  C40B10/00 ,  C12N1/20 ,  C07K1/00 ,  C07K14/00 ,  C12P21/09

CPC分类号： C12R1/19 ,  C12N9/1014 ,  C12N9/80 ,  C12N15/1058 ,  C12N15/70 ,  C12P21/02 ,  C12Y305/01088 ,  C40B10/00

摘要： The present invention relates to producing non-formylated proteins and/or peptides in bacterial cells lacking deformylase and/or transformylase.

摘要翻译： 本发明涉及在缺乏脱甲酰酶和/或转化酶的细菌细胞中产生非甲酰化的蛋白质和/或肽。

公开(公告)号：US12125561B2

公开(公告)日：2024-10-22

申请号：US16143708

申请日：2018-09-27

申请人： InnoSIGN B.V.

发明人： Meng Dou ,  Wilhelmus Franciscus Johannes Verhaegh ,  Anja Van De Stolpe ,  Rick Velter

IPC分类号： G16B5/00 ,  C12Q1/6851 ,  C12Q1/6886 ,  C40B10/00 ,  C40B20/00 ,  G01N33/68 ,  G16B5/20 ,  G16B20/00 ,  G16B25/00

CPC分类号： G16B5/00 ,  C12Q1/6851 ,  C12Q1/6886 ,  C40B10/00 ,  C40B20/00 ,  G01N33/6872 ,  G16B5/20 ,  G16B20/00 ,  G16B25/00 ,  C12N2501/42 ,  C12Q2600/158 ,  G01N2333/4703 ,  G01N2800/52 ,  C12Q1/6851 ,  C12Q2537/165

摘要： A bioinformatics process which provides an improved means to detect a JAK-STAT3 cellular signaling pathway in a subject, such as a human, based on the expression levels of at least three unique target genes of the JAK-STAT3 cellular signaling pathway measured in a sample. The invention includes an apparatus comprising a digital processor configured to perform such a method, a non-transitory storage medium storing instructions that are executable by a digital processing device to perform such a method, and a computer program comprising program code means for causing a digital processing device to perform such a method. Kits are also provided for measuring expression levels of unique sets of JAK-STAT3 cellular signaling pathway target genes.

公开(公告)号：US20230374491A1

公开(公告)日：2023-11-23

申请号：US18329296

申请日：2023-06-05

申请人： ETH ZURICH

发明人： Sai REDDY ,  William KELTON ,  Cristina PAROLA ,  Derek MASON ,  Mark POGSON

IPC分类号： C12N15/10 ,  C12N5/0781 ,  C12N9/22 ,  C12N15/113 ,  C40B10/00 ,  C40B20/04 ,  C40B40/08

CPC分类号： C12N15/1037 ,  C12N5/0635 ,  C12N9/22 ,  C12N15/102 ,  C12N15/113 ,  C12N15/1082 ,  C12N15/1058 ,  C12N15/1086 ,  C40B10/00 ,  C40B20/04 ,  C40B40/08 ,  C12N2310/20 ,  C12N2510/02 ,  C12N2800/80 ,  C12N15/1034 ,  C12N2510/00 ,  C12Q2521/539

摘要： According to a first aspect of the invention, a method for the generation of a cell line is provided, comprising the steps of (a) providing a plurality of mammalian B cells, wherein each of the plurality of B cells comprises a transgenic genomic DNA sequence encoding a marker protein inserted into an endogenous immunoglobulin locus comprised in said B cell, and wherein the transgenic genomic DNA sequence is amenable to cleavage by a site directed nuclease, particularly Cas9; (b) replacing the transgenic genomic DNA sequence encoding a marker protein with a second transgenic DNA sequence encoding a protein of interest; (c) sorting B cells based on the presence or absence of the marker protein; and (d) collecting B cells in which the marker protein is absent.

公开(公告)号：US11332735B2

公开(公告)日：2022-05-17

申请号：US16732710

申请日：2020-01-02

申请人： Full Spectrum Genetics, Inc.

发明人： Robert DuBridge

IPC分类号： C40B30/04 ,  C12N15/10 ,  C07K1/04 ,  C07K16/00 ,  C40B10/00 ,  C40B40/02 ,  C40B40/10

摘要： The invention provides efficient methods for combining single-substitution libraries of nucleic acids that span and encode proteins of interest and for selecting resultant mutant proteins after expression which have improved properties or characteristics.

公开(公告)号：US20200095704A1

公开(公告)日：2020-03-26

申请号：US16513945

申请日：2019-07-17

申请人： President and Fellows of Harvard College

发明人： George M. Church ,  Francois Vigneault ,  Uri Laserson ,  Ido Bachelet

IPC分类号： C40B10/00 ,  C12Q1/6881 ,  C12N15/10 ,  C07K16/00 ,  C40B30/04 ,  C40B40/08 ,  G16B30/00 ,  G16B20/00 ,  C12Q1/6806

摘要： Methods and compositions for determining and/or monitoring the immune state of an individual are provided.

公开(公告)号：US20190316275A1

公开(公告)日：2019-10-17

申请号：US16445121

申请日：2019-06-18

申请人： IDEA ORCHARD, LLC

发明人： Michael Longo ,  Rajika Perera

IPC分类号： C40B40/10 ,  C12N15/10 ,  C40B10/00 ,  C40B40/08

摘要： A general method and recombinant nucleic acid sequences, by means which the method selects a recombinant protein containing an FHA domain for binding a target molecule from a library proteins with a high-throughput method of creating protein variations within the FHA domain in non-conserved or non-structural sequences of the FHA scaffold, and the library may also be in the form of a phagemid or phage library wherein the ALP nucleic acid sequence is inserted into a vector capable of allowing the vector and expressed ALP protein from being virally packaged, and the recombinant nucleic acid sequences which are randomly mutated at varying non-conserved or non-structural FHA domain sequences.

公开(公告)号：US20180216097A1

公开(公告)日：2018-08-02

申请号：US15747282

申请日：2016-07-25

申请人： Manel CAMPS

发明人： Manel CAMPS

IPC分类号： C12N15/10 ,  C12N9/12 ,  C40B10/00 ,  C12P19/34 ,  C12N15/70

CPC分类号： C12N15/1024 ,  C12N9/1252 ,  C12N15/102 ,  C12N15/70 ,  C12P19/34 ,  C40B10/00

摘要： A system for continuous mutagenesis to facilitate directed evolution, the system including DNA polymerases carrying the novel K54E point mutation, and other point mutations including I709N, A759R, D424A (herein called K54E_LF Pol I) and this methods of use to produce and detect lines where mutagenesis is continuous and does not exhibit the usual decline in mutagenesis with sequential cloning.

公开(公告)号：US09920453B2

公开(公告)日：2018-03-20

申请号：US14741265

申请日：2015-06-16

申请人： ProteoNova, Inc

发明人： Richard B Williams

IPC分类号： C40B50/06 ,  C40B40/02 ,  C07K14/00 ,  C12N15/10 ,  C40B10/00 ,  C40B40/08

CPC分类号： C40B40/02 ,  C07K14/003 ,  C12N15/1037 ,  C12N15/1062 ,  C12N15/1075 ,  C40B10/00 ,  C40B40/08 ,  C40B50/06

摘要： Methods and compositions are provided for producing libraries of soluble random polypeptides. In the methods, the fraction of hydrophilic residues in the polypeptide is controlled so as to maintain the solubility of the polypeptide constructs.

公开(公告)号：US09528106B2

公开(公告)日：2016-12-27

申请号：US13733473

申请日：2013-01-03

申请人： Medical Research Council

发明人： Andrew Griffiths ,  Dan Tawfik

IPC分类号： C12Q1/68 ,  C12Q1/00 ,  C12Q1/70 ,  C12P21/06 ,  C40B10/00 ,  C40B30/08 ,  C40B30/10 ,  C12N15/10

CPC分类号： C12N15/1058 ,  C12N15/1062 ,  C12N15/1075 ,  C12Q1/6811 ,  C12Q2563/159

摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising of the steps of: (a) compartmentalizing genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within the microcapsules; (c) sorting the genetic elements which produce the gene product having a desired activity. The invention enables the in vitro evolution of nucleic acids by repeated mutagenesis and iterative applications of the method of the invention.

摘要翻译： 本发明描述了一种用于分离编码具有所需活性的基因产物的一种或多种遗传元件的方法，包括以下步骤：（a）将遗传元件分隔成微胶囊; （b）表达遗传元件以在微胶囊内产生它们各自的基因产物; （c）分选产生具有所需活性的基因产物的遗传元件。 本发明能够通过本发明方法的重复诱变和迭代应用来体外进化核酸。