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公开(公告)号:US08846410B2
公开(公告)日:2014-09-30
申请号:US14081376
申请日:2013-11-15
IPC分类号: G01N15/06 , G01N33/00 , G01N33/48 , B01L3/00 , B03C1/02 , B01F13/00 , B01F11/00 , B01L7/00 , G01N35/10
CPC分类号: G01N33/54333 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B03C1/02 , B03C1/288 , B03C1/30 , B03C5/005 , B03C2201/18 , B03C2201/26 , C12N15/1013 , G01N33/561 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要: The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
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公开(公告)号:US20140069812A1
公开(公告)日:2014-03-13
申请号:US14081376
申请日:2013-11-15
IPC分类号: B03C1/02
CPC分类号: G01N33/54333 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B03C1/02 , B03C1/288 , B03C1/30 , B03C5/005 , B03C2201/18 , B03C2201/26 , C12N15/1013 , G01N33/561 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要: The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
摘要翻译: 本发明涉及在液滴致动器上的珠孵化和洗涤。 提供了用于将用一抗标记的磁响应珠,样品(即分析物)和二次报告基因抗体孵育在磁体上,磁铁上和从磁体上离开并完全离开磁体的方法。 还提供了使用形状辅助的液滴合并来洗涤磁响应珠的方法。 还提供了用于形状介导的分离,输送和分配含有磁响应珠的样品液滴的方法。 本发明的装置和方法提供了免疫测定中分析物的快速结果和最佳检测的时间。
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公开(公告)号:US08541176B2
公开(公告)日:2013-09-24
申请号:US12113385
申请日:2008-05-01
IPC分类号: G01N33/53
CPC分类号: G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of modifying a surface on a droplet microactuator is provided, wherein the method includes executing one or more droplet operations to bring a droplet comprising a surface-modifying agent into contact with the surface. According to another embodiment, a droplet microactuator is provided and includes a sample or reagent immobilized on a surface thereof and arranged such that a droplet on the droplet microactuator may contact the surface. According to yet another embodiment, a method of removing a substance from a surface to which the substance is bound is provided, the method including conducting one or more droplet operations to contact a droplet with the surface, the droplet comprising a solution for eluting the substance from the surface
摘要翻译: 本发明涉及液滴表面改性和洗涤。 根据一个实施例,提供了修改液滴微致动器上的表面的方法,其中所述方法包括执行一个或多个液滴操作以使包含表面改性剂的液滴与表面接触。 根据另一个实施例,提供液滴微致动器,并且包括固定在其表面上的样品或试剂,并且布置成使得液滴微致动器上的液滴可接触表面。 根据另一个实施方案,提供从物质结合的表面除去物质的方法,所述方法包括进行一个或多个液滴操作以使液滴与表面接触,所述液滴包含用于洗脱物质的溶液 从表面
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公开(公告)号:US20130146461A1
公开(公告)日:2013-06-13
申请号:US13686495
申请日:2012-11-27
IPC分类号: C25B15/00
CPC分类号: C25B15/00 , B01F13/0071 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/0647 , B01L2200/0668 , B01L2200/0673 , B01L2300/0636 , B01L2300/0816 , B01L2300/0861 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , B01L2400/0487 , G01N33/5438
摘要: A droplet actuator and method of providing a droplet comprising an target substance on the droplet actuator, and including discrete flow and continuous flow functionality. Discrete flow function is controlled by electrodes arranged for conducting droplet operations on a substrate surface. The continuous flow function includes a fluid path arranged for flowing a fluid therethrough. The discrete flow and continuous flow functions may be by a barrier, including a second fluid path through the barrier. The continuous flow function may include a capture surface comprising a component having an affinity for the target substance. Methods of the invention may include flowing a fluid comprising the target substance through the fluid path; capturing the target substance on the capture surface; and forming a droplet in the discrete flow region via the second fluid path and the electrodes comprising the captured target substance.
摘要翻译: 液滴致动器和在液滴致动器上提供包含目标物质的液滴的方法,并且包括离散流动和连续流动功能。 离散流动功能由布置用于在基板表面上进行液滴操作的电极控制。 连续流动功能包括设置成使流体流过其中的流体路径。 离散的流动和连续流动功能可以是通过屏障,包括通过屏障的第二流体路径。 连续流动功能可以包括包含对目标物质具有亲和性的成分的捕获表面。 本发明的方法可以包括使包含目标物质的流体流过流体路径; 捕获表面上的目标物质; 以及经由所述第二流体路径在所述离散流动区域中形成液滴,并且所述电极包括所捕获的目标物质。
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公开(公告)号:US20130118901A1
公开(公告)日:2013-05-16
申请号:US13737433
申请日:2013-01-09
IPC分类号: G01N27/447
CPC分类号: G01N27/44769 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0663 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/1827 , B01L2400/0415 , B01L2400/0424 , B81B3/0021 , B81B2201/058 , B81C1/00119 , C12Q1/6846 , G01N27/447 , G01N27/44791 , G05D7/0694 , H01L21/6715 , Y10T436/2575
摘要: Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.
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公开(公告)号:US20130092539A1
公开(公告)日:2013-04-18
申请号:US13680979
申请日:2012-11-19
IPC分类号: G01N27/447
CPC分类号: C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要: Aspects of embodiments of the invention relate to a simulator including a visual display capable of outputting to a user a display one or more effects of a command series selected and a system including a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator including a substrate comprising electrodes for conducting droplet operations. Further aspects of embodiments of the invention relate to a droplet operations troubleshooting apparatus. Other aspects of embodiments of the invention relate to a computer implemented method of displaying simulated microactuator droplets.
摘要翻译: 本发明的实施例的方面涉及一种模拟器,其包括能够向用户输出显示所选择的命令序列的一个或多个效果的视觉显示器,以及包括电子耦合到能够执行指令的处理器并由其控制的液滴微致动器的系统 液滴微致动器包括包括用于进行液滴操作的电极的基板。 本发明的实施例的其它方面涉及一种液滴操作故障排除装置。 本发明的实施例的其他方面涉及一种显示模拟微致动器液滴的计算机实现的方法。
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公开(公告)号:US08389297B2
公开(公告)日:2013-03-05
申请号:US11639822
申请日:2006-12-15
IPC分类号: G01N33/553
CPC分类号: C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要: The present invention relates to a droplet-based affinity assay device and system. According to one embodiment, a droplet microactuator is provided and includes an antibody immobilized on a surface. According to another embodiment, a droplet microactuator is provided and includes a droplet on the droplet microactuator, the droplet comprising an antibody.
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公开(公告)号:US08317990B2
公开(公告)日:2012-11-27
申请号:US12531835
申请日:2008-03-24
IPC分类号: G01N35/08 , G01N33/543
CPC分类号: C25B15/00 , B01F13/0071 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/0647 , B01L2200/0668 , B01L2200/0673 , B01L2300/0636 , B01L2300/0816 , B01L2300/0861 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , B01L2400/0487 , G01N33/5438
摘要: A droplet actuator and method of providing a droplet comprising an target substance on the droplet actuator, and including discrete flow and continuous flow functionality. Discrete flow function is controlled by electrodes arranged for conducting droplet operations on a substrate surface. The continuous flow function includes a fluid path arranged for flowing a fluid therethrough. The discrete flow and continuous flow functions may be by a barrier, including a second fluid path through the barrier. The continuous flow function may include a capture surface comprising a component having an affinity for the target substance. Methods of the invention may include flowing a fluid comprising the target substance through the fluid path; capturing the target substance on the capture surface; and forming a droplet in the discrete flow region via the second fluid path and the electrodes comprising the captured target substance.
摘要翻译: 液滴致动器和在液滴致动器上提供包含目标物质的液滴的方法,并且包括离散流动和连续流动功能。 离散流动功能由布置用于在基板表面上进行液滴操作的电极控制。 连续流动功能包括设置成使流体流过其中的流体路径。 离散的流动和连续流动功能可以是通过屏障,包括通过屏障的第二流体路径。 连续流动功能可以包括包含对目标物质具有亲和性的成分的捕获表面。 本发明的方法可以包括使包含目标物质的流体流过流体路径; 捕获表面上的目标物质; 以及经由所述第二流体路径在所述离散流动区域中形成液滴,并且所述电极包括所捕获的目标物质。
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公开(公告)号:US08313895B2
公开(公告)日:2012-11-20
申请号:US12615609
申请日:2009-11-10
IPC分类号: C12Q1/00
CPC分类号: G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a bead-containing droplet with a reduced concentration of a substance is provided, the method including providing a droplet microactuator including a droplet having a starting volume and including one or more beads and a starting concentration and starting quantity of the substance. The method further includes conducting one or more droplet operations to merge a wash droplet with the droplet provided in the above step to yield a combined droplet and including one or more droplet operations to divide the combined droplet to yield a set of droplets including a droplet including substantially all of the one or more beads and having a decreased concentration of the substance relative to the starting concentration and a droplet which is substantially lacking in the beads.
摘要翻译: 本发明涉及液滴表面改性和洗涤。 根据一个实施例,提供了一种提供具有降低的物质浓度的含珠粒的液滴的方法,所述方法包括提供包括具有起始体积并包括一个或多个珠粒和起始浓度的液滴的液滴微致动器,并起始浓度 物质的数量。 该方法还包括进行一个或多个液滴操作以将洗涤液滴与上述步骤中提供的液滴合并以产生组合液滴并且包括一个或多个液滴操作以分开组合的液滴以产生包括液滴的一组液滴,包括液滴,包括 基本上所有的一种或多种珠粒,并且物质相对于起始浓度降低,而珠子中基本上不含有液滴。
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公开(公告)号:US08313698B2
公开(公告)日:2012-11-20
申请号:US12960583
申请日:2010-12-06
CPC分类号: C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要: The present invention relates to a droplet-based nucleic acid amplification apparatus and system. According to one embodiment, a droplet microactuator is provided made using a first substrate including a fluorescing material and including a detection region for detecting a fluorescence signal from a droplet, which detection region is coated with a light absorbing, low fluorescence or non-fluorescing material.
摘要翻译: 本发明涉及一种基于液滴的核酸扩增装置和系统。 根据一个实施例,使用包括荧光材料的第一基底并且包括用于检测来自液滴的荧光信号的检测区域来提供液滴微致动器,该检测区域被光吸收,低荧光或非荧光材料涂覆 。
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