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公开(公告)号：US20100151502A1
公开(公告)日：2010-06-17
申请号：US12295889
申请日：2007-04-05
申请人： Bernhard Hauer , Dietmar Häring , Cecilia Kvarnstroem-Branneby , Rolf Schmid , Monika Müller , Danni Liu
发明人： Bernhard Hauer , Dietmar Häring , Cecilia Kvarnstroem-Branneby , Rolf Schmid , Monika Müller , Danni Liu
IPC分类号： G01N33/554 , C12N9/20 , C07H21/00 , C12N15/74 , C12N1/21
CPC分类号： C12N9/20 , C12Q1/44 , C12Q1/61 , G01N2333/918
摘要： The present invention relates to nucleic acids that code for triacylglycerol lipases, vectors comprising said nucleic acids, host cells comprising said nucleic acids or vectors, methods for the expression of triacylglycerol lipases in prokaryotes, methods for the detection and for the production of triacylglycerol lipases, triacylglycerol lipases obtainable thereby, and the use of triacylglycerol lipase-encoding nucleic acids, vectors and recombinant host cells for said methods.
摘要翻译： 本发明涉及编码三酰基甘油脂肪酶的核酸，包含所述核酸的载体，包含所述核酸或载体的宿主细胞，在原核生物中表达三酰甘油脂肪酶的方法，用于检测和产生三酰甘油脂肪酶的方法， 由此获得的三酰基甘油脂酶，以及用于所述方法的用于酰基甘油脂肪酶编码核酸，载体和重组宿主细胞的用途。
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公开(公告)号：US07714038B2
公开(公告)日：2010-05-11
申请号：US11817509
申请日：2006-03-02
申请人： Dietmar Haering , Bernhard Hauer , Thomas Pfeiffer , Andreas Fechtenkoetter , Harald Larbig , Erich Beck
发明人： Dietmar Haering , Bernhard Hauer , Thomas Pfeiffer , Andreas Fechtenkoetter , Harald Larbig , Erich Beck
CPC分类号： C09D11/12 , C08F8/00 , C08F210/02 , C08F255/02 , C08L23/0869 , C09D7/65 , C08F10/00 , C08F220/00 , C08F218/04 , C08F2500/02 , C08F220/12
摘要： Polyolefin waxes bearing (meth)acrylate groups, process for preparing them and their use.
摘要翻译： （甲基）丙烯酸酯基聚烯烃蜡，它们的制备方法及用途。
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公开(公告)号：US20100035315A1
公开(公告)日：2010-02-11
申请号：US12514215
申请日：2007-11-13
申请人： Rainer Stürmer , Bernhard Hauer , Thomas Friedrich , Kurt Faber , Melanie Hall
发明人： Rainer Stürmer , Bernhard Hauer , Thomas Friedrich , Kurt Faber , Melanie Hall
IPC分类号： C12P7/26
CPC分类号： C12P7/24 , C12P7/26 , C12P7/40 , C12P7/46 , C12P7/62 , C12P13/008 , C12P17/10 , C12P41/002
摘要： A method for enzymatic preparation of compounds of the general formula (2) from unsaturated alkene derivatives of the general formula (1) by reducing a compound of the formula (1) in the presence of a reductase, comprising at least one of the polypeptide sequences SEQ ID NO: 1, 2 or 3 or having a functionally equivalent polypeptide sequence which is at least 80% identical to SEQ ID NO: 1, 2 or 3.
摘要翻译： 一种通过在还原酶的存在下还原式（1）的化合物，从通式（1）的不饱和烯烃衍生物中酶制备通式（2）的化合物的方法，所述方法包括至少一种多肽序列 具有与SEQ ID NO：1,2或3至少80％相同的功能等同多肽序列的SEQ ID NO：1,2或3。
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84.发明申请PROCESS FOR THE PREPARATION OF OPTICALLY ACTIVE 5-SUBSTITUTED 2-OXAZOLIDINONES FROM RACEMIC EPOXIDES AND CYANATE EMPLOYING A HALOHYDRIN DEHALOGENASE 失效用于制备光化活性的5-取代的2-取代的二氧杂环己烷的方法来自环氧化物和使用氢化物脱氢酶的氰酸酯公开(公告)号：US20090042261A1
公开(公告)日：2009-02-12
申请号：US12279786
申请日：2007-02-27
申请人： Bernhard Hauer
发明人： Bernhard Hauer
IPC分类号： C12P17/14
CPC分类号： C12P17/14 , C12P41/006
摘要： A process for the production of an optically enriched oxazolidinone of the formula (2a) or (2b), by reacting an epoxide of the formula (1) with cyanate in the presence of halo-hydrin dehalogenase.
摘要翻译： 通过式（1）的环氧化物与卤代 - 卤素脱卤素酶的存在下的氰酸酯反应制备式（2a）或（2b）的光学富集的恶唑烷酮的方法。
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85.发明授权Method for analyzing enzyme-catalyzed reactions using MALDI-TOF mass spectrometry 失效使用MALDI-TOF质谱法分析酶催化反应的方法公开(公告)号：US07445885B2
公开(公告)日：2008-11-04
申请号：US10296381
申请日：2001-06-06
CPC分类号： H01J49/04
摘要： A process is described for analyzing enzyme-catalyzed conversions of nonpolymeric substrates to nonpolymeric products with the aid of MALDI-TOF mass spectrometry, preferably in the presence of an internal standard on a specific carrier material.
摘要翻译： 描述了一种方法，用于借助MALDI-TOF质谱法，优选在特定载体材料上存在内标物的情况下分析非聚合物底物对非聚合物产物的酶催化转化。
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公开(公告)号：US20080200631A1
公开(公告)日：2008-08-21
申请号：US11995628
申请日：2006-07-31
申请人： Dietmar Haring , Uwe Meisenburg , Bernhard Hauer , Frank Dietsche
发明人： Dietmar Haring , Uwe Meisenburg , Bernhard Hauer , Frank Dietsche
IPC分类号： C08F118/02
CPC分类号： C12P7/625
摘要： The present invention relates to a process for preparing (meth)acrylic esters of alcohols having C—C triple bonds, to processes for their preparation, and to their use.
摘要翻译： 本发明涉及一种制备具有C-C三键的醇的（甲基）丙烯酸酯的方法，其制备方法及其用途。
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公开(公告)号：US07314739B2
公开(公告)日：2008-01-01
申请号：US10493210
申请日：2002-10-17
CPC分类号： C12N9/20
摘要： The invention relates to a lipase variant which can be prepared by carrying out, on the amino acid sequence of a starting lipase selected from the lipase homologous family I.1 or I.2, at least one amino acid substitution in those positions which correspond to the positions 17, 29, 30, 52, 86, 117, 122, 160, 163, 167, 265, 266, 286, 289 in the prototype lipase sequence SEQ ID NO: 1.
摘要翻译： 本发明涉及脂酶变体，其可通过在选自脂肪酶同源家族I.1或I.2的起始脂肪酶的氨基酸序列上进行至少一个氨基酸取代而制备， 原型脂肪酶序列SEQ ID NO：1中的位置17,29,30,25,66,117,122,160,163,167,265,266,286,289。
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88.发明申请Process for an Enzymatic Oxygenation by Direct Electrochemical Regeneration of the Fad-Dependent Monooxygenase 审中-公开通过直接电化学再生法自由基单氧化酶进行酶促氧化的方法公开(公告)号：US20070231871A1
公开(公告)日：2007-10-04
申请号：US11596301
申请日：2005-05-11
IPC分类号： C12P1/00
CPC分类号： C12P1/00 , C12N9/0071 , C12P7/00
摘要： Processes comprising oxygenating an educt to form a product in the presence of an FAD-dependent monooxygenase, wherein the FAD-dependent monooxygenase is regenerated by direct electrochemical reduction are described.
摘要翻译： 描述了通过在FAD依赖性单加氧酶存在下使排气物氧化以形成产物的方法，其中通过直接电化学还原再生FAD依赖性单加氧酶。
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89.发明授权Method for producing chiral carboxylic acids from nitriles with the assistance of a nitrilase or microoganisms which contain a gene for the nitrilase 有权在腈水解酶或含有腈水解酶基因的微生物的帮助下，由腈生产手性羧酸的方法公开(公告)号：US06869783B1
公开(公告)日：2005-03-22
申请号：US09806876
申请日：1999-10-13
IPC分类号： C12N15/09 , C07H21/00 , C12N1/21 , C12N9/78 , C12N15/55 , C12P7/40 , C12P7/42 , C12P41/00 , C12R1/05 , C12P7/62
CPC分类号： C07H21/00 , C12N9/78 , C12P7/42 , C12P41/006
摘要： The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.
摘要翻译： 本发明涉及编码具有腈水解酶活性的多肽的核酸序列，包含核酸序列的核酸构建体以及包含核酸序列或核酸构建体的载体。 本发明还涉及由核酸序列编码的氨基酸序列，以及包含核酸序列，核酸构建体或包含核酸序列或核酸构建体的载体的微生物。本发明还涉及一种方法 用于从外消旋腈制备手性羧酸。
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公开(公告)号：US20050048484A1
公开(公告)日：2005-03-03
申请号：US10398178
申请日：2001-10-16
申请人： Bernhard Hauer , Rolf Schmid , Rainer Merkl , Francesca Blasco
发明人： Bernhard Hauer , Rolf Schmid , Rainer Merkl , Francesca Blasco
IPC分类号： C12N15/09 , C12M1/40 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/53 , C12P1/04 , C12P7/00 , C12R1/01 , C12Q1/68 , C07H21/04 , C12N15/74
CPC分类号： C12N9/0077 , C12P1/04
摘要： The invention relates to novel cytochrome P450 monooxygenases from thermophilic bacteria, in particular the genus Thermus sp., to nucleotide sequences encoding them, to the recombinant production of these monooxygenases and to their use for the microbiological oxidation of organic compounds.
摘要翻译： 本发明涉及嗜热细菌，特别是栖热菌属的新型细胞色素P450单加氧酶与编码它们的核苷酸序列，重组生产这些单加氧酶及其用于微生物氧化有机化合物的新型细胞色素P450单加氧酶。
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