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89 条记录 (耗时 0.117 秒)

    Methods and Compositions for Efficient Nucleic Acid Sequencing
    82.
    发明申请
    Methods and Compositions for Efficient Nucleic Acid Sequencing 审中-公开
    高效核酸测序的方法和组合

    公开(公告)号：US20080108074A1

    公开(公告)日：2008-05-08

    申请号：US11929038

    申请日：2007-10-30

    申请人： Radoje Drmanac

    发明人： Radoje Drmanac

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： C12Q1/6874

    摘要： Disclosed are novel methods and compositions for rapid and highly efficient nucleic acid sequencing based upon hybridization with two sets of small oligonucleotide probes of known sequences. Extremely large nucleic acid molecules, including chromosomes and non-amplified RNA, may be sequenced without prior cloning or subcloning steps. The methods of the invention also solve various current problems associated with sequencing technology such as, for example, high noise to signal ratios and difficult discrimination, attaching many nucleic acid fragments to a surface, preparing many, longer or more complex probes and labelling more species.

    摘要翻译： 公开了基于与已知序列的两组小寡核苷酸探针杂交的用于快速和高效核酸测序的新颖方法和组合物。 非常大的核酸分子，包括染色体和非扩增的RNA，可以在没有先前的克隆或亚克隆步骤的情况下进行测序。 本发明的方法还解决了与排序技术相关的各种当前问题，例如高噪声与信号比和困难的鉴别，将许多核酸片段连接到表面，制备许多更长或更复杂的探针并标记更多的物质 。

    Large-scale parallelized DNA sequencing
    84.
    发明申请
    Large-scale parallelized DNA sequencing 审中-公开

    公开(公告)号：US20060110764A1

    公开(公告)日：2006-05-25

    申请号：US11281188

    申请日：2005-11-16

    申请人： Tom Tang ,  Radoje Drmanac

    发明人： Tom Tang ,  Radoje Drmanac

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6874 ,  C12Q2600/156 ,  C12Q2525/179 ,  C12Q2527/101 ,  C12Q2535/101 ,  C12Q2565/125 ,  C12Q2565/537

    摘要： We provide a DNA sequencing method and a sequencing system where large numbers of sequence reads can be obtained in parallel by running traditional electrophoresis in a special format. Parallelization is obtained either through a 3-dimensional gel-cube or through bundled capillary tubes including fiber-optic tubes or other types of micro channels in a bundle or matrix format. Various ways of capturing sequence traces are provided. We also provide two distinct methods for preparing genomic DNA/cDNA fragments: one through universal primer site anchoring and amplification of single molecules, and the other through micro-array/bead oligomer extension and dye-terminator incorporation using target sequence specific primers. The invention can perform large-scale genomic sequencing including sequencing a complete human genome in one or a few runs.

    Large-scale parallelized DNA sequencing
    85.
    发明申请
    Large-scale parallelized DNA sequencing 审中-公开
    大规模并行DNA测序

    公开(公告)号：US20060110756A1

    公开(公告)日：2006-05-25

    申请号：US11258775

    申请日：2005-10-25

    申请人： Tom Tang ,  Radoje Drmanac

    发明人： Tom Tang ,  Radoje Drmanac

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： C12Q1/6874 ,  C12Q2600/156 ,  C12Q2525/179 ,  C12Q2527/101 ,  C12Q2535/101 ,  C12Q2565/125 ,  C12Q2565/537

    摘要： We provide a DNA sequencing method and a sequencing system where large numbers of sequence reads can be obtained in parallel by running traditional electrophoresis in a special format. Parallelization is obtained either through a 3-dimensional gel-cube or through bundled capillary tubes including fiber-optic tubes or other types of micro channels in a bundle or matrix format. Various ways of capturing sequence traces are provided. We also provide two distinct methods for preparing genomic DNA/cDNA fragments: one through universal primer site anchoring and amplification of single molecules, and the other through micro-array/bead oligomer extension and dye-terminator incorporation using target sequence specific primers. The invention can perform large-scale genomic sequencing including sequencing a complete human genome in one or a few runs.

    摘要翻译： 我们提供DNA测序方法和测序系统，其中通过以特殊格式运行传统电泳可以并行获得大量的序列读数。 通过三维凝胶立方体或通过捆束毛细管（包括光纤管或其他类型的微通道以束或矩阵形式）获得平行化。 提供了捕获序列轨迹的各种方式。 我们还提供了两种不同的制备基因组DNA / cDNA片段的方法：一个通过通用引物位点锚定和扩增单个分子，另一个通过使用靶序列特异性引物的微阵列/珠寡聚体延伸和染料终止子掺入。 本发明可以进行大规模的基因组测序，包括在一个或几个运行中测序完整的人类基因组。