公开(公告)号：US10350281B2

公开(公告)日：2019-07-16

申请号：US15802350

申请日：2017-11-02

申请人： VETERINARMEDIZINISCHE UNIVERSITAT WIEN

发明人： Michael Hess ,  Petra Ganas

IPC分类号： A61K39/002 ,  C12N1/10 ,  C12N1/36 ,  A61K39/108 ,  C12N1/20 ,  A61K39/00

摘要： The invention discloses a method for producing a single bacterial strain culture of Histomonas meleagridis (H. meleagridis), the method being characterised by the following steps: (a) providing a xenic culture of H. meleagridis comprising H. meleagridis cells with a wild type bacterial flora, (b) treating the xenic culture with a mixture of antibiotics thereby killing the wild type bacterial flora, (c) centrifuging and washing the H. meleagridis cells, (d) controlling effectiveness of step (b), (e) resuspending the washed H. meleagridis cells, (f) adding one or more single bacterial strain(s) to the resuspended H. meleagridis cells, and (g) culturing the one or more single bacterial strain(s) with the resuspended H. meleagridis cells so as to obtain a single bacterial strain culture of H. meleagridis. The invention further discloses a vaccine formulation consisting of a Histomonas component consisting of an attenuated culture of Histomonas meleagridis, a bacterial component consisting of one or more cultures of a single bacterial strain, and pharmaceutically acceptable non-biological formulation compounds.

公开(公告)号：US10208305B2

公开(公告)日：2019-02-19

申请号：US14130769

申请日：2012-07-03

申请人： Jeannie T. Lee ,  Yesu Jeon

发明人： Jeannie T. Lee ,  Yesu Jeon

IPC分类号： C12N1/10 ,  G01N33/50 ,  C40B30/00 ,  G01N33/574 ,  C12N15/10 ,  G01N33/68

摘要： Methods relating to obtaining libraries of YY1-binding long non-coding RNAs, libraries obtained thereby, and methods of use thereof.

公开(公告)号：US20180236051A1

公开(公告)日：2018-08-23

申请号：US15752140

申请日：2016-08-12

申请人： BIOTICK PESQUISA E DESENVOLVIMENTO TECNOLÓGICO LTDA ,  FUNDACÃO BUTANTAN

发明人： Darci Moraes Barros Battesti ,  Irina Kerkis ,  Ronaldo Zucatelli Mendonca ,  Durvanei Augusto Maria ,  Angelina Cirelli Moraes

IPC分类号： A61K39/00 ,  C12N1/10 ,  C12N1/20 ,  G01N33/53 ,  C12N5/07 ,  A61K35/646 ,  A61K39/02

CPC分类号： A61K39/0003 ,  A61K35/56 ,  A61K35/646 ,  A61K39/00 ,  A61K39/0233 ,  C07K14/29 ,  C12N1/10 ,  C12N1/20 ,  C12N5/0601 ,  G01N33/53

摘要： The invention refers to a process for the production of a continuous cell line of simile embryo stem cells, such as Amblyomma sculptum (Acari: Ixodidae), known as line IBU/ASE-16 (access number with CNCM: 1-5000) and its uses. More specifically, the invention refers to a process for the production of the line IBU/ASE-16 and their use for obtaining extracts for the production of vaccines and candidate recombinant proteins for biopharmaceuticals and acaricides, production of diagnostic kits for the detection of antigens and/or antibodies for animal and human use, obtaining clones for use in genotyping and use as a substrate for the isolation and culture of pathogens.

公开(公告)号：US20180117131A1

公开(公告)日：2018-05-03

申请号：US15802350

申请日：2017-11-02

申请人： VETERINARMEDIZINISCHE UNIVERSITAT WIEN

发明人： Michael Hess ,  Petra Ganas

IPC分类号： A61K39/002 ,  C12N1/36 ,  C12N1/20 ,  C12N1/10 ,  A61K39/108 ,  A61K39/00

CPC分类号： A61K39/002 ,  A61K39/00 ,  A61K39/0258 ,  C12N1/10 ,  C12N1/20 ,  C12N1/36

摘要： The invention discloses a method for producing a single bacterial strain culture of Histomonas meleagridis (H. meleagridis), the method being characterised by the following steps: (a) providing a xenic culture of H. meleagridis comprising H. meleagridis cells with a wild type bacterial flora, (b) treating the xenic culture with a mixture of antibiotics thereby killing the wild type bacterial flora, (c) centrifuging and washing the H. meleagridis cells, (d) controlling effectiveness of step (b), (e) resuspending the washed H. meleagridis cells, (f) adding one or more single bacterial strain(s) to the resuspended H. meleagridis cells, and (g) culturing the one or more single bacterial strain(s) with the resuspended H. meleagridis cells so as to obtain a single bacterial strain culture of H. meleagridis. The invention further discloses a vaccine formulation consisting of a Histomonas component consisting of an attenuated culture of Histomonas meleagridis, a bacterial component consisting of one or more cultures of a single bacterial strain, and pharmaceutically acceptable non-biological formulation compounds.

公开(公告)号：US20180112175A1

公开(公告)日：2018-04-26

申请号：US15571280

申请日：2016-05-06

申请人： Riken ,  eugiena Co,. Ltd.

发明人： Takashi Osanai ,  Masami Hirai ,  Hiroko IIjima ,  Yuka Nakaya ,  Kengo Suzuki ,  Osamu Iwata ,  Ayaka Nakashima

IPC分类号： C12N1/10 ,  C12P7/46

CPC分类号： C12N1/10 ,  C12P7/40 ,  C12P7/46

摘要： The present invention provides a method for producing an organic acid using algal biomass, The present invention pertains to the production of organic acids such as succinic acid using Euglena , by means of a method containing; either a nitrogen deficient culture step in which Euglena is cultured. aerobically under nitrogen-deficient conditions or a heterotrophic culture step in which Euglena is cultured aerobically using a culture medium containing a carbon source; and an anaerobic culture step in which the culture product obtained in the nitrogen-deficient culture step is incubated under anaerobic conditions.

公开(公告)号：US20170355951A1

公开(公告)日：2017-12-14

申请号：US15621875

申请日：2017-06-13

申请人： Sanaria Inc. ,  Johns Hopkins University

发明人： Russell H. TAYLOR ,  Amanda CANEZIN ,  Mariah SCHRAM ,  lulian IORDACHITA ,  Gregory CHIRIKJIAN ,  Michelle LASKOWSKI ,  Sumana CHAKRAVARTY ,  Stephen HOFFMAN

IPC分类号： C12N3/00 ,  C12N1/10 ,  C12M1/00

CPC分类号： C12N3/00 ,  C12M47/04 ,  C12N1/10

摘要： A device and method of use for augmenting the extraction of salivary glands from Plasmodium-infected mosquitoes, where the sporozoite stage of Plasmodium primarily resides. Sporozoites are useful for research as well as for the immunogen in whole parasite vaccines for the prevention of malaria. The device and methods of use disclosed herein greatly increase the rate at which sporozoites can be harvested.

公开(公告)号：US20170191023A1

公开(公告)日：2017-07-06

申请号：US15400224

申请日：2017-01-06

申请人： DSM IP ASSETS B.V.

发明人： James Casey LIPPMEIER ,  Kirk E. Apt

IPC分类号： C12N1/10 ,  C12N15/52 ,  A23K50/00 ,  A23L33/10 ,  A23K50/80 ,  C12N1/22 ,  A23L2/52

CPC分类号： C12N1/10 ,  A23K50/00 ,  A23K50/80 ,  A23L2/52 ,  A23L33/10 ,  A23V2002/00 ,  C12N1/22 ,  C12N15/52 ,  C12N15/79 ,  C12P7/649 ,  Y02E50/13

摘要： The present invention is directed to recombinant thraustochytrids that grow on xylose and cell cultures comprising the recombinant thraustochytrids as well as methods of producing cell cultures, biomasses, microbial oils, compositions, and biofuels using the recombinant thraustochytrids.

公开(公告)号：US09528083B2

公开(公告)日：2016-12-27

申请号：US15017354

申请日：2016-02-05

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Clinton C. Staheli ,  Nephi D. Jones ,  Michael E. Goodwin

IPC分类号： C12N5/10 ,  C12M1/00 ,  B01F3/04 ,  B01F7/16 ,  B01F7/18 ,  B01F15/00 ,  C12M1/06 ,  C12M1/12 ,  B01D47/05 ,  C12N1/10 ,  C12N1/12 ,  C12N1/14 ,  C12N1/20 ,  C12N5/00 ,  C12N5/04 ,  B01D5/00 ,  B01F7/00 ,  B01F15/06

CPC分类号： C12M23/14 ,  B01D5/0072 ,  B01D47/05 ,  B01F3/04241 ,  B01F3/04531 ,  B01F7/00691 ,  B01F7/1695 ,  B01F7/18 ,  B01F7/22 ,  B01F15/00071 ,  B01F15/00831 ,  B01F15/0085 ,  B01F15/065 ,  B01F2003/04191 ,  B01F2003/04297 ,  B01F2003/04673 ,  B01F2003/04865 ,  B01F2003/04872 ,  B01F2003/04879 ,  B01F2003/04893 ,  B01F2003/04921 ,  B01F2015/061 ,  B01F2215/0073 ,  C12M23/26 ,  C12M23/34 ,  C12M27/02 ,  C12M29/00 ,  C12M29/06 ,  C12M29/24 ,  C12M37/00 ,  C12M41/14 ,  C12M41/18 ,  C12N1/10 ,  C12N1/12 ,  C12N1/14 ,  C12N1/20 ,  C12N5/00 ,  C12N5/04 ,  C12N5/10 ,  F28B1/02 ,  F28F3/12 ,  F28F21/06 ,  F28F2255/02

摘要： A heat exchanger system includes a heat exchanger having a body with a first side face and an opposing second side face, the body bounding a fluid channel disposed between the opposing side faces and extending between an inlet port and an outlet port. A flexible first bag has of one or more sheet of polymeric material and bounds a fluid pathway that extends between a fluid inlet and a fluid outlet. The first bag is removably retained against the first side face of the body of the heat exchanger, the fluid channel of the body of the heat exchanger being sealed from fluid communication with the fluid pathway of the first bag.

摘要翻译： 热交换器系统包括具有主体的热交换器，该主体具有第一侧面和相对的第二侧面，该主体包围设置在相对侧面之间并在入口和出口之间延伸的流体通道。 柔性第一袋具有一个或多个聚合物材料片并且限定了在流体入口和流体出口之间延伸的流体路径。 第一袋可移除地保持在热交换器的主体的第一侧面上，热交换器的主体的流体通道与第一袋的流体通道的流体连通密封。

公开(公告)号：US09463229B2

公开(公告)日：2016-10-11

申请号：US13132468

申请日：2009-12-02

申请人： Hugo Daniel Lujan

发明人： Hugo Daniel Lujan

IPC分类号： C12N1/10 ,  C12N1/00 ,  A61K39/00 ,  A01N63/00 ,  A61K48/00 ,  A61K39/002 ,  A61K35/68 ,  C07K14/44 ,  C07K16/20 ,  C12N15/86 ,  A61K38/00 ,  C12N1/20

CPC分类号： A61K39/002 ,  A61K35/68 ,  A61K38/00 ,  A61K2039/522 ,  A61K2039/542 ,  A61K2039/552 ,  C07K14/44 ,  C07K16/20 ,  C12N1/10 ,  C12N1/20 ,  C12N15/86 ,  Y02A50/491

摘要： Modified protozoa parasites comprising simultaneous expression on its surface of at least two variable surface proteins (VSP). The modified protozoa may also simultaneously express the complete repertoire of variable surface proteins. Protozoa show reduced expression of Dicer, RNA-dependant RNA-polymerase (RdRP) enzymes or both, wherein the RdRP gene and/or the Dicer gene has been silenced. The protozoan may be any protozoan showing an antigenic variation mechanism.

摘要翻译： 修饰的原生动物寄生虫包括在其表面上同时表达至少两种可变表面蛋白（VSP）。 修饰的原生动物也可以同时表达可变表面蛋白的完整谱。 原生动物显示Dicer，RNA依赖性RNA聚合酶（RdRP）酶或两者的表达降低，其中RdRP基因和/或Dicer基因已被沉默。 原生动物可以是显示抗原变异机制的任何原生动物。

公开(公告)号：US20160287688A1

公开(公告)日：2016-10-06

申请号：US15095360

申请日：2016-04-11

申请人： SANARIA INC.

发明人： Abraham G. EAPPEN ,  Stephen L. HOFFMAN

IPC分类号： A61K39/015 ,  C12N1/10

CPC分类号： A61K39/015 ,  A61K2039/52 ,  A61K2039/522 ,  C12N1/10 ,  Y02A50/412

摘要： The application is directed to in vitro-reared Plasmodium sporozoites of human host range wherein sporogony from gametocyte stage to sporozoite stage is external to mosquitoes, and methods of producing the same. Provided herein are in vitro-reared infectious Plasmodium sporozoites (SPZ) of human host range, particularly P. falciparum, P. vivax, P. ovale, P. malariae, and P. knowlesi, wherein sporogony from gametocyte stage to sporozoite stage is external to mosquitoes, and methods of producing the same.

摘要翻译： 该应用涉及人宿主范围的体外培养的疟原虫子孢子，其中从配子细胞阶段到子孢子期的孢子体在蚊子外部，以及其生产方法。 本文提供的是人宿主范围的体外培养的感染性疟原虫子孢子（SPZ），特别是恶性疟原虫，间日疟原虫，卵白念珠菌，疟原虫和know know属，其中从配子细胞阶段到子孢子期的孢子是外部的 蚊子，以及生产蚊子的方法。