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    FLUORESCENT DYES AND METHODS OF USE THEREOF
    2.
    发明申请
    FLUORESCENT DYES AND METHODS OF USE THEREOF 有权

    公开(公告)号：US20210079034A1

    公开(公告)日：2021-03-18

    申请号：US16952399

    申请日：2020-11-19

    申请人： Enzo Life Sciences, Inc.

    发明人： Zaiguo Li ,  Praveen Pande

    IPC分类号： C07H21/00 ,  C12Q1/70 ,  C09B11/24 ,  C07H19/04 ,  C09B11/02 ,  C09B11/06

    摘要： Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.

    SELF-IMMOLATIVE PROBES FOR ENZYME ACTIVITY DETECTION
    5.
    发明申请
    SELF-IMMOLATIVE PROBES FOR ENZYME ACTIVITY DETECTION 审中-公开

    公开(公告)号：US20190119719A1

    公开(公告)日：2019-04-25

    申请号：US16177676

    申请日：2018-11-01

    申请人： ENZO LIFE SCIENCES, INC.

    发明人： Irina Lebedeva ,  Wayne Forrest Patton ,  Maciej Szczepanik ,  Yuejun Xiang ,  Praveen Pande

    IPC分类号： C12Q1/26 ,  C12Q1/48 ,  C07D311/08 ,  C07D491/052 ,  C07D491/20 ,  C09K11/06 ,  C12Q1/44 ,  C12Q1/37 ,  C07D493/10

    摘要： Provided is a compound having the structure: (SIG)-(SI-MOD)m where SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample, such as a cell, comprises an activator, such as a nitroreducase, using the compound. Further provided are methods of determining whether a mammalian cell is hypoxic using the compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase using the compound where nitroreductase is the activator is also provided.

    Multisignal reagents for labeling analytes
    6.
    发明授权
    Multisignal reagents for labeling analytes 有权

    公开(公告)号：US10184934B2

    公开(公告)日：2019-01-22

    申请号：US15609360

    申请日：2017-05-31

    申请人： Enzo Life Science, Inc.

    发明人： Jack Coleman ,  Maciej Szczepanik ,  Richard Jin

    IPC分类号： G01N33/53 ,  C07H21/00 ,  C07H21/04 ,  C09B11/08 ,  C09B11/24 ,  C12Q1/6818 ,  G01N33/58 ,  G01N33/82 ,  G01N33/94

    摘要： Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. Additionally provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.