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公开(公告)号：US20230146066A1
公开(公告)日：2023-05-11
申请号：US17920661
申请日：2021-04-05
发明人： Ayumi GA , Yoshiya TOMIMORI , Hisataka YASUDA
IPC分类号： C12N5/00
CPC分类号： C12N5/0018 , C12N2500/40 , C12N2500/50 , C12N2500/34
摘要： A medium for stem cells according to the present invention contains at least one of carboxymethyl cellulose and polyvinylpyrrolidone as a water-soluble polymer. The content of carboxymethyl cellulose in the medium is preferably such that the final concentration thereof is 0.001 μg/mL to 1 mg/mL. The content of polyvinylpyrrolidone in the medium is preferably such that the final concentration thereof is 0.05 μg/mL to 2 mg/mL.
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公开(公告)号：US20130164839A1
公开(公告)日：2013-06-27
申请号：US13638172
申请日：2011-03-31
IPC分类号： C12N5/071 , C12N5/0735 , C12N5/0775 , C12N5/0786 , C12N5/0797 , C12N5/077 , C12N5/078 , C12N5/09 , C12N5/0789
CPC分类号： C07K14/78 , C12N5/0068 , C12N2533/52
摘要： The present invention is directed to providing a method for culturing cells in a system containing laminin-5. The method of the present invention is characterized by a culture system containing a polypeptide selected from a group consisting of: a protein in blood other than extracellular matrix proteins, which is, serum, serum albumin, prealbumin, immunoglobulin, α-globulin, β-globulin, α1-antitrypsin (α1-AT), heptoglobin (Hp), α2-macroglobulin (α2-M), α-fetoprotein (AFP), transferrin, retinol-binding protein (RBP) or adiponectin; gelatin; a protein belonging to a tumor necrosis factor (TNF) family; and peptone.
摘要翻译： 本发明旨在提供一种在含有层粘连蛋白-5的系统中培养细胞的方法。 本发明的方法的特征在于包含选自以下的多肽的培养系统：除了细胞外基质蛋白之外的血液中的蛋白质，即血清，血清白蛋白，前白蛋白，免疫球蛋白，α-球蛋白，β- 球蛋白，α1-抗胰蛋白酶（α1-AT），肝球蛋白（Hp），α2-巨球蛋白（α2-M），甲胎蛋白（AFP），转铁蛋白，视黄醇结合蛋白（RBP）或脂联素; 明胶; 属于肿瘤坏死因子（TNF）家族的蛋白质; 和蛋白胨。
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公开(公告)号：US20120276531A1
公开(公告)日：2012-11-01
申请号：US13415508
申请日：2012-03-08
申请人： Toshiyuki Saito , Yoji Mikami , Masahiro Kinugasa , Kazuya Mori , Michiyo Sugimoto , Koji Uchida
发明人： Toshiyuki Saito , Yoji Mikami , Masahiro Kinugasa , Kazuya Mori , Michiyo Sugimoto , Koji Uchida
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/158
摘要： To provide a novel method for determining the risk of lymph node metastasis of breast cancer uses as an index the difference in the expression levels of marker genes in at least one material selected from the group consisting of a breast tissue and a breast cell of a patient. The method includes measuring an expression level of a marker gene in at least one material selected from the group consisting of a breast tissue and a breast cell of a patient with breast cancer, and determining the risk of lymph node metastasis of breast cancer in the patient using the expression level of the marker gene as an index.
摘要翻译： 提供一种用于确定乳腺癌用途的淋巴结转移的风险的新方法作为指标，其选自由患者的乳房组织和乳腺细胞组成的组中的至少一种材料中的标记基因的表达水平的差异 。 该方法包括测量在选自乳腺组织和乳腺癌患者的乳腺细胞的至少一种材料中的标记基因的表达水平，并确定患者中乳腺癌淋巴结转移的风险 使用标记基因的表达水平作为指标。
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公开(公告)号：US20110117645A1
公开(公告)日：2011-05-19
申请号：US12922244
申请日：2009-03-31
IPC分类号： C12N5/071
CPC分类号： C12N5/0606 , C12N5/0696 , C12N2500/10 , C12N2500/12 , C12N2500/20 , C12N2500/25 , C12N2500/32 , C12N2500/98 , C12N2533/52
摘要： For efficient proliferation of pluripotent stem cells in a system free from any animal-derived material such as feeder cells or serum, the present invention provides a method for proliferation of pluripotent stem cells, which comprises culturing the pluripotent stem cells in a medium free from both feeder cells and serum in a system containing laminin-5.
摘要翻译： 为了在不含任何动物来源的材料如饲养细胞或血清的系统中有效增殖多能干细胞，本发明提供了多能干细胞增殖的方法，其包括在不含两者的培养基中培养多能干细胞 饲养细胞和血清在含有层粘连蛋白-5的系统中。
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公开(公告)号：US07858751B2
公开(公告)日：2010-12-28
申请号：US12008279
申请日：2008-01-10
申请人： Kinji Ito , Toshiharu Ishii , Yuri Fukasawa
发明人： Kinji Ito , Toshiharu Ishii , Yuri Fukasawa
摘要： It is intended to provide a specific peptide fragment of LYVE-1 which can serve as an epitope of a lymphatic vessel-specific antibody, and an antibody recognizing the above peptide fragment. A peptide fragment comprising an amino acid sequence: Ser Lys Lys Thr Asp Lys Asn Pro Glu Glu Ser Lys, or the peptide fragment in which an amino acid as a linker to the C-terminal or the N-terminal thereof is added.
摘要翻译： 旨在提供可用作淋巴管特异性抗体的表位的LYVE-1的特异性肽片段和识别上述肽片段的抗体。 包含氨基酸序列：Ser Lys Lys Thr Asp Lys Asn Pro Glu Glu Ser Lys的肽片段或其中加入作为C末端或N-末端的连接体的氨基酸的肽片段。
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公开(公告)号：US20100209566A1
公开(公告)日：2010-08-19
申请号：US12524735
申请日：2008-01-30
申请人： Takeo Yano , Yumiko Sakai , Kouji Uchida , Yoshiki Nakao , Kimie Ishihata , Shigeru Nakano , Toshihiro Yamada
发明人： Takeo Yano , Yumiko Sakai , Kouji Uchida , Yoshiki Nakao , Kimie Ishihata , Shigeru Nakano , Toshihiro Yamada
IPC分类号： G01N33/02
CPC分类号： C12Q1/6895 , A01H1/04 , C07K14/415
摘要： [Object] To newly develop a method whereby walnut can be simply, rapidly and accurately separated and distinguished from pecan nut.[Means for Resolution] Due to the increase in the number of patients with the walnut allergen and the increase of import of pecan nut which is an edible nut belonging to the family Juglandaceae, recently in Japan, the development of a method for detecting walnut and the nut in a discriminative manner is urgently needed. With attention focused on the chloroplast matK gene with the apparent gene sequence, we developed a PCR method for specifically detecting nuts of the family Juglandaceae and identified the matK sequence of pecan nut to establish a method for discriminating walnut and pecan nut from each other using restriction enzymes, for discriminating walnut and pecan nut from each other.
摘要翻译： [对象]新开发一种核桃可以简单，快速，准确地分离和区别于山核桃坚果的方法。 [解决方法]由于核桃过敏原患者数量的增加以及最近在日本属于菊科植物的可食用坚果的山核桃进口量的增加，开发了检测核桃的方法 迫切需要有歧视的手段。 对于叶绿体matK基因与表观基因序列的关注，我们开发了一种特异性检测菊科植物坚果的PCR方法，并确定了山核桃坚果的matK序列，建立了一种用核桃和山核桃相互鉴别核桃和山核桃的方法 用于区分核桃和山核桃果仁的酶。
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公开(公告)号：US20100086939A1
公开(公告)日：2010-04-08
申请号：US12530711
申请日：2008-02-15
申请人： Hiroshi Kido , Koichi Suzuki
发明人： Hiroshi Kido , Koichi Suzuki
IPC分类号： G01N33/53
CPC分类号： G01N33/6854 , G01N33/54373
摘要： It is to provide a method for determining allergic diseases with a high sensitivity and accuracy which enables multilateral and global analysis with a minute amount of sample, even using body fluid other than blood such as saliva, nasal discharge and tears as a sample, particularly by suppressing nonspecific reaction as much as possible. A chemically modified diamond/DLC (Diamond-like Carbon) chip is activated with a reacting reagent, a coupling reaction with a peptide comprising allergen or allergen epitope is conducted, and a sample such as saliva, tears, and nasal discharge which has undergone pressure filtration with a low protein-adsorbing filter, is contacted with an allergen determination chip to which washing and blocking operations have been performed on unreacted active groups, and an allergen recognizing antibody in the sample captured by the allergen determination chip is detected by immunoassay using a labeled secondary antibody, wherein a glycine-containing solution is used for a washing solution and/or blocking solution used in the washing and blocking operations, is used.
摘要翻译： 它提供了一种以高灵敏度和准确度确定过敏性疾病的方法，即使使用除血液以外的体液（如唾液，鼻腔排出物和眼泪）作为样品，也可以使用少量样品进行多边和全球分析，特别是通过 尽可能地抑制非特异性反应。 用反应试剂激活化学改性的金刚石/ DLC（类金刚石碳）芯片，进行与包含变应原或过敏原表位的肽的偶联反应，以及经历压力的样品如唾液，泪液和鼻腔排出物 与低蛋白质吸附过滤器的过滤与已对未反应的活性基团进行洗涤和封闭操作的变应原测定芯片接触，并且通过免疫测定法检测由变应原测定芯片捕获的样品中的变应原识别抗体，使用 使用其中含有甘氨酸的溶液用于洗涤和阻塞操作中使用的洗涤溶液和/或封闭溶液。
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公开(公告)号：US07598038B2
公开(公告)日：2009-10-06
申请号：US11662781
申请日：2005-09-15
CPC分类号： C12Q1/6895 , C12Q2600/158
摘要： The invention provides a means for estimating the end use qualities of wheat flour that will be obtained in the future from the harvested wheat at an early stage before maturation of the wheat seeds. The invention relates to a method for estimating the end use qualities of a matured wheat seed, comprising measuring the expression level of at least 1 gene selected from genes, each of which is defined by any one of the nucleotide sequences of SEQ ID NOS: 1 to 121 in immature wheat.
摘要翻译： 本发明提供了一种在小麦种子成熟前的早期阶段从收获的小麦获得的小麦面粉的最终使用质量的估算方法。 本发明涉及一种用于估计成熟小麦种子的最终用途品质的方法，包括测量选自基因中的至少1个基因的表达水平，每个基因由SEQ ID NO：1的任一核苷酸序列 到未成熟小麦的121只。
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公开(公告)号：US07339022B2
公开(公告)日：2008-03-04
申请号：US10548495
申请日：2004-03-11
申请人： Kinji Ito , Toshiharu Ishii , Yuri Fukasawa
发明人： Kinji Ito , Toshiharu Ishii , Yuri Fukasawa
IPC分类号： A61K38/00
摘要： It is intended to provide a specific peptide fragment of LYVE-1 which can serve as an epitope of a lymphatic vessel-specific antibody, and an antibody recognizing the above peptide fragment. A peptide fragment comprising an amino acid sequence:Ser Lys Lys Thr Asp Lys Asn Pro Glu Glu Ser Lys, or the peptide fragment in which an amino acid as a linker to the C-terminal or the N-terminal thereof is added.
摘要翻译： 旨在提供可用作淋巴管特异性抗体的表位的LYVE-1的特异性肽片段和识别上述肽片段的抗体。 包含氨基酸序列：Ser Lys Lys Thr Asp Lys Asn Pro Glu Glu Ser Lys的肽片段或其中加入作为C末端或N-末端的连接体的氨基酸的肽片段。
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公开(公告)号：US5827710A
公开(公告)日：1998-10-27
申请号：US869506
申请日：1997-06-05
申请人： Kohji Uchida , Hirokazu Matsukawa , Tuyosi Fujita , Yushi Matuo
发明人： Kohji Uchida , Hirokazu Matsukawa , Tuyosi Fujita , Yushi Matuo
CPC分类号： C12N9/0006 , C12Y101/01027 , C12Y101/01028
摘要： The present invention provides a gene encoding the type B subunit protein of avian lactate dehydrogenase. The gene encoding the type B subunit protein of avian lactate dehydrogenase of the present invention is obtained by plaque hybridization or the like from a cDNA library derived from avian heart muscle and encodes the type B subunit protein of avian lactate dehydrogenase having the sequences shown as SEQ ID NO. 1 or 2.
摘要翻译： 本发明提供编码禽类乳酸脱氢酶的B型亚基蛋白的基因。 编码本发明禽类乳酸脱氢酶B型亚基蛋白的基因是通过来自禽心肌的cDNA文库的斑块杂交等获得的，并编码具有SEQ所示序列的禽类乳酸脱氢酶的B亚基蛋白 证件号码。 1或2。
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