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    Methods for increasing the production of phenolic compounds from Theobroma cacao
    4.
    发明授权
    Methods for increasing the production of phenolic compounds from Theobroma cacao 有权
    从可可可可酚中提高酚类化合物的生产方法

    公开(公告)号：US09428759B2

    公开(公告)日：2016-08-30

    申请号：US13951619

    申请日：2013-07-26

    申请人： Raul Cuero Rengifo

    发明人： Raul Cuero Rengifo

    IPC分类号： C12N9/88 ,  C12N15/82

    CPC分类号： C12N15/8243 ,  C12N9/88

    摘要： Described herein are methods for enhancing the production of phenolic compounds from Theobroma cacao. For example, the devices and methods described herein increase the production of phenolic compounds from cocoa plants, which has industrial and economic value. The phenolic compounds produced by the devices and methods do not require the ultra purification that is common in conventional or commercial methods. The devices and methods described herein also enhance the growth rate of plants.

    摘要翻译： 本文描述了用于增强可可可可酚的酚类化合物的生产的方法。 例如，本文所述的装置和方法增加了具有工业和经济价值的可可植物的酚类化合物的生产。 由装置和方法生产的酚类化合物不需要在常规或商业方法中常见的超纯化。 本文所述的装置和方法还增强了植物的生长速率。

    METHODS AND DEVICES FOR ELECTROMAGNETIC AMPLIFICATION OF NUCLEIC ACIDS
    5.
    发明申请
    METHODS AND DEVICES FOR ELECTROMAGNETIC AMPLIFICATION OF NUCLEIC ACIDS 有权
    用于核酸的电磁放大的方法和装置

    公开(公告)号：US20140030764A1

    公开(公告)日：2014-01-30

    申请号：US13839325

    申请日：2013-03-15

    申请人： Raul Cuero RENGIFO ,  Mario Antonio FRANCO JIMENEZ ,  Natalia GUTIERREZ CALLE ,  Mariana SANCHEZ LONDONO

    发明人： Raul Cuero RENGIFO ,  Mario Antonio FRANCO JIMENEZ ,  Natalia GUTIERREZ CALLE ,  Mariana SANCHEZ LONDONO

    IPC分类号： B01J19/08

    CPC分类号： B01J19/087 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2523/305

    摘要： Methods and devices for amplifying nucleic acids generally involve exposing the nucleic acid to an electromagnetic field, for example a mini-current magnetic field, while performing the steps of PCR. The PCR methods and devices provide numerous advantages over conventional PCR techniques and systems such as reduced reaction times, no heating requirements, and reduced amounts of reagents (e.g., optional use polymerases and primers). Additionally, the PCR methods and devices require significantly shorter reaction times (e.g., less than one hour) compared to conventional PCR techniques and systems (minimum 2 hours). Finally, as shown in the Examples, the PCR methods and devices amplify significantly more DNA compared to conventional PCR techniques and systems. Accordingly, the PCR methods and devices provide a more efficient and cost-effective way to perform PCR when compared to conventional PCR techniques and systems.

    摘要翻译： 用于扩增核酸的方法和装置通常涉及将核酸暴露于电磁场，例如微电流磁场，同时执行PCR步骤。 与常规PCR技术和系统相比，PCR方法和装置提供了许多优点，例如减少的反应时间，无加热要求和减少量的试剂（例如任选的使用聚合酶和引物）。 另外，与常规PCR技术和系统（最少2小时）相比，PCR方法和装置需要显着更短的反应时间（例如，小于1小时）。 最后，如实施例所示，与常规PCR技术和系统相比，PCR方法和装置显着增加DNA。 因此，与常规PCR技术和系统相比，PCR方法和装置提供了更有效和成本有效的方式进行PCR。

    METHODS AND DEVICES FOR ELECTROMAGNETIC LIGATION OF NUCLEIC ACIDS AND ELECTROMAGNETIC TRANSFORMATION OF CELLS
    10.
    发明申请
    METHODS AND DEVICES FOR ELECTROMAGNETIC LIGATION OF NUCLEIC ACIDS AND ELECTROMAGNETIC TRANSFORMATION OF CELLS 审中-公开
    核酸的电磁化学和细胞的电磁转化的方法和装置

    公开(公告)号：US20160168578A1

    公开(公告)日：2016-06-16

    申请号：US14909173

    申请日：2014-07-31

    申请人： Raul CUERO RENGIFO ,  Natalia GUTIERREZ CALLE ,  Mariana SANCHEZ LONDONO ,  INTERNATIONL PARK OF CREATIVITY

    发明人： Raul Cuero RENGIFO ,  Natalia GUTIERREZ CALLE ,  Mariana SANCHEZ LONDONO

    IPC分类号： C12N15/64 ,  C12P19/34

    CPC分类号： C12N15/64 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q2521/501 ,  C12Q2523/303

    摘要： Described herein are methods and devices for ligating nucleic acids or nucleic acid fragments as well as methods for transforming or transfecting cells with exogenous DNA. The methods and devices generally involve exposing the nucleic acids, nucleic acid fragments, and/or cells to an electromagnetic field, for example, a mini-current electromagnetic field, while performing the steps of ligation or transformation. The methods and devices provide numerous advantages over conventional ligation and transformation techniques and systems such as reduced reaction times, no heating requirements, and reduced amounts of reagents. Finally, as shown in the examples below, the methods and devices described herein are more effective compared to conventional ligation and transformation techniques and systems.

    摘要翻译： 本文描述了用于连接核酸或核酸片段的方法和装置以及用外源DNA转化或转染细胞的方法。 所述方法和装置通常涉及将核酸，核酸片段和/或细胞暴露于电磁场，例如小电流电磁场，同时执行连接或转化步骤。 与传统的连接和转化技术和系统相比，这些方法和装置提供了许多优点，例如减少的反应时间，不需要加热和减少试剂量。 最后，如以下实施例所示，与常规连接和转化技术和系统相比，本文所述的方法和装置更有效。