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    Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses
    6.
    发明授权
    Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses 有权
    用于分离蛋白质或蛋白质和核酸关联的方法，或颗粒和蛋白质复合物，试剂和用途

    公开(公告)号：US07700744B2

    公开(公告)日：2010-04-20

    申请号：US11320574

    申请日：2005-12-30

    Applicant: Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    Inventor： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    IPC: A23J1/00

    CPC classification number: C12N15/1013 ,  C07K1/22 ,  G01N33/5434

    Abstract: The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.

    Abstract translation: 磁性胶体颗粒包括核心和外壳，其中芯是磁性的并且涂覆有至少一种包含选自胺，羟基，硫醇，醛，酯，酸酐，酰氯，碳酸酯，氨基甲酸酯，异氰酸酯的官能团X的聚合物 和异硫氰酸酯基团或其混合物，其至少一部分已经与外壳的其它官能团反应，并且外壳包含可离子化官能团Z和Z'的聚合物，Z和Z'可以相同或不同，选自胺 ，羧酸，酯，酸酐，醛，硫醇，二硫化物，α-卤代羰基，磺酸，马来酰亚胺，异氰酸酯和异硫氰酸酯基团，其与核的官能团X部分反应。 这些磁性胶体颗粒可用于分离生物材料。

    Reagent and method for the detection of a nucleotide sequence with signal amplification
    7.
    发明授权
    Reagent and method for the detection of a nucleotide sequence with signal amplification 失效
    用于检测具有信号放大的核苷酸序列的试剂和方法

    公开(公告)号：US6017707A

    公开(公告)日：2000-01-25

    申请号：US870730

    申请日：1997-06-06

    Applicant: Bernard Mandrand ,  Philippe Cros ,  Thierry Delair ,  Marie-Helene Charles ,  Marie-Noelle Erout ,  Christian Pichot

    Inventor： Bernard Mandrand ,  Philippe Cros ,  Thierry Delair ,  Marie-Helene Charles ,  Marie-Noelle Erout ,  Christian Pichot

    IPC: C08G81/02 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12N15/00

    CPC classification number: C12Q1/6813 ,  C08G81/02 ,  C12Q1/682

    Abstract: Methods for the detection of a nucleotide sequence of interest comprising at least one nucleotide probe marked with a tracer. The methods include a reagent essentially comprising a linear backbone copolymer having lateral substituents, whose chain consists of a first type of repetitive unit and at least one other type of repetitive unit, in which at least one part of the units of the first type have a lateral substituent comprising a nucleotide unit, such a lateral substituent not being present on the other types of units. Each of said nucleotide units, all of which are identical, comprise at least one nucleotide sequence capable of hybridizing with said sequence of interest and nucleotide sequence capable of hybridizing with a probe, the reagent containing on average more than two of said nucleotide units, in molar equivalents, per mole of polymer. Such a reagent enables signal amplification to be obtained, and therefor lowers the sensitivity threshold. Application, in particular, in the production of tests for the detection of pathogenic organisms, or in the diagnosis of genetic diseases.

    Abstract translation: 检测目的核苷酸序列的方法包括用示踪剂标记的至少一个核苷酸探针。 所述方法包括基本上包含具有侧向取代基的线性主链共聚物的试剂，其链由第一类型的重复单元和至少一种其它类型的重复单元组成，其中第一类型的单元的至少一部分具有 包含核苷酸单元的侧向取代基，这样的侧向取代基不存在于其它类型的单元上。 所述全部相同的所述核苷酸单元包含至少一个能够与所述感兴趣序列杂交的核苷酸序列和能够与探针杂交的核苷酸序列，所述试剂平均包含两个以上的所述核苷酸单元， 摩尔当量，每摩尔聚合物。 这样的试剂能够获得信号放大，从而降低灵敏度阈值。 特别是应用于生产用于检测病原体的检测或遗传疾病的诊断。

    Reagent and method for the detection of a nucleotide sequence with signal amplification
    9.
    发明授权
    Reagent and method for the detection of a nucleotide sequence with signal amplification 失效
    用于检测具有信号放大的核苷酸序列的试剂和方法

    公开(公告)号：US5695936A

    公开(公告)日：1997-12-09

    申请号：US433505

    申请日：1995-06-14

    Applicant: Bernard Mandrand ,  Philippe Cros ,  Thierry Delair ,  Marie-Helene Charles ,  Marie-Noelle Erout ,  Christian Pichot

    Inventor： Bernard Mandrand ,  Philippe Cros ,  Thierry Delair ,  Marie-Helene Charles ,  Marie-Noelle Erout ,  Christian Pichot

    IPC: C08G81/02 ,  C12Q1/68

    CPC classification number: C12Q1/6813 ,  C08G81/02 ,  C12Q1/682

    Abstract: Methods for the detection of a nucleotide sequence of interest comprising at least one nucleotide probe marked with a tracer. The methods comprise use of a reagent essentially comprising a linear backbone copolymer having lateral substituents, whose chain consists of a first type of repetitive unit and at least one other type of repetitive unit, in which at least one part of the units of the first type have a lateral substituent comprising a nucleotide unit, such a lateral substituent not being present on the other types of units. Each of said nucleotide units, all of which are identical, comprise at least one nucleotide sequence capable of hybridizing with said sequence of interest and nucleotide sequence capable of hybridizing with a probe, the reagent containing on average more than two of said nucleotide units, in molar equivalents, per mole of polymer. Such a reagent enables signal amplification to be obtained, and therefor lowers the sensitivity threshold. Application, in particular, in the production of tests for the detection of pathogenic organisms, or in the diagnosis of genetic diseases.

    Abstract translation: PCT No.PCT / FR94 / 01084第 371 1995年6月14日第 102（e）1995年6月14日PCT PCT 1994年9月15日PCT公布。 WO95 / 08000 PCT出版物 1995年3月23日的方法检测目标核苷酸序列的方法包括用示踪剂标记的至少一个核苷酸探针。 所述方法包括使用基本上包含具有侧向取代基的线性主链共聚物的试剂，其链由第一类型的重复单元和至少一种其它类型的重复单元组成，其中第一类型的单元的至少一部分 具有包含核苷酸单元的侧向取代基，这样的侧向取代基不存在于其它类型的单元上。 所述全部相同的所述核苷酸单元包含至少一个能够与所述感兴趣序列杂交的核苷酸序列和能够与探针杂交的核苷酸序列，所述试剂平均包含两个以上的所述核苷酸单元， 摩尔当量，每摩尔聚合物。 这样的试剂能够获得信号放大，从而降低灵敏度阈值。 特别是应用于生产用于检测病原体的检测或遗传疾病的诊断。

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