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18 条记录 (耗时 0.061 秒)

    Method and apparatus for determining copy number variation using digital PCR
    5.
    发明授权
    Method and apparatus for determining copy number variation using digital PCR 有权
    使用数字PCR确定拷贝数变异的方法和装置

    公开(公告)号：US09487822B2

    公开(公告)日：2016-11-08

    申请号：US12170414

    申请日：2008-07-09

    申请人： Simant Dube ,  Jian Qin ,  Ramesh Ramakrishnan

    发明人： Simant Dube ,  Jian Qin ,  Ramesh Ramakrishnan

    IPC分类号： G01N33/483 ,  C12Q1/68 ,  G01N33/00 ,  B01L3/00 ,  G01N35/00

    CPC分类号： C12Q1/6837 ,  B01L3/5027 ,  G01N35/00594 ,  Y10T436/143333 ,  C12Q2527/137 ,  C12Q2537/16 ,  C12Q2537/165

    摘要： A method of estimating a concentration of DNA molecules in a biological sample includes storing a number of a plurality of reaction sites in a memory and distributing the biological sample among the plurality of reaction sites. The method also includes determining a number of the plurality of reaction sites characterized by a presence of one or more of the DNA molecules and computing a portion of the plurality of reaction sites characterized by the presence of the one or more of the DNA molecules. The method further includes estimating the concentration of the DNA molecules as a function of the portion of the plurality of reaction sites and computing a confidence interval for the estimated concentration of DNA molecules.

    摘要翻译： 估计生物样品中DNA分子浓度的方法包括将多个反应位点存储在存储器中并在多个反应位点之间分配生物样品。 该方法还包括确定多个反应位点的数目，其特征在于存在一个或多个DNA分子，并计算以一个或多个DNA分子存在为特征的多个反应位点的一部分。 该方法还包括估计DNA分子的浓度作为多个反应位点的一部分的函数，并计算DNA分子的估计浓度的置信区间。

    METHOD AND APPARATUS FOR DETERMINING COPY NUMBER VARIATION USING DIGITAL PCR
    7.
    发明申请
    METHOD AND APPARATUS FOR DETERMINING COPY NUMBER VARIATION USING DIGITAL PCR 有权
    使用数字PCR确定复制数变化的方法和装置

    公开(公告)号：US20090239308A1

    公开(公告)日：2009-09-24

    申请号：US12170414

    申请日：2008-07-09

    申请人： Simant Dube ,  Jian Qin ,  Ramesh Ramakrishnan

    发明人： Simant Dube ,  Jian Qin ,  Ramesh Ramakrishnan

    IPC分类号： G01N33/483 ,  C12M1/34

    CPC分类号： C12Q1/6837 ,  B01L3/5027 ,  G01N35/00594 ,  Y10T436/143333 ,  C12Q2527/137 ,  C12Q2537/16 ,  C12Q2537/165

    摘要： A method of estimating a concentration of DNA molecules in a biological sample includes storing a number of a plurality of reaction sites in a memory and distributing the biological sample among the plurality of reaction sites. The method also includes determining a number of the plurality of reaction sites characterized by a presence of one or more of the DNA molecules and computing a portion of the plurality of reaction sites characterized by the presence of the one or more of the DNA molecules. The method further includes estimating the concentration of the DNA molecules as a function of the portion of the plurality of reaction sites and computing a confidence interval for the estimated concentration of DNA molecules.

    摘要翻译： 估计生物样品中DNA分子浓度的方法包括将多个反应位点存储在存储器中并在多个反应位点之间分配生物样品。 该方法还包括确定多个反应位点的数目，其特征在于DNA分子中存在一个或多个DNA分子，并计算以一个或多个DNA分子存在为特征的多个反应位点的一部分。 该方法还包括估计DNA分子的浓度作为多个反应位点的一部分的函数，并计算DNA分子的估计浓度的置信区间。

    Methods for detecting multiple target nucleic acids in multiple samples by use nucleotide tags
    8.
    发明授权
    Methods for detecting multiple target nucleic acids in multiple samples by use nucleotide tags 有权
    通过使用核苷酸标签检测多个样品中的多个靶核酸的方法

    公开(公告)号：US08697363B2

    公开(公告)日：2014-04-15

    申请号：US12548132

    申请日：2009-08-26

    申请人： Alain Mir ,  Ramesh Ramakrishnan ,  Marc Unger ,  Bernhard G. Zimmermann

    发明人： Alain Mir ,  Ramesh Ramakrishnan ,  Marc Unger ,  Bernhard G. Zimmermann

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/686 ,  C12N15/1065 ,  C12Q1/6853 ,  Y10T436/143333 ,  C12Q2537/143 ,  C12Q2525/161

    摘要： The present invention provides assay methods that increase the number of samples and/or target nucleic acids that can be analyzed in a single assay. In certain embodiments, an assay method entails separately subjecting S samples to an encoding reaction that produces a set of T tagged target nucleotide sequences, each tagged target nucleotide sequence including a sample-specific nucleotide tag and a target nucleotide sequence. In some embodiments, an assay method entails separately subjecting S samples to an encoding reaction that produces a set of T tagged target nucleotide sequences, each tagged target nucleotide sequence including a first nucleotide tag linked to a target nucleotide sequence, which is linked to a second nucleotide tag. In either case, the tagged target nucleotide sequences from the S samples can be mixed to form an assay mixture and subsequently assayed.

    摘要翻译： 本发明提供了增加可以在单次测定中分析的样品和/或靶核酸数量的测定方法。 在某些实施方案中，测定方法需要分别使S样品经受产生一组T标记的靶核苷酸序列的编码反应，每个标记的靶核苷酸序列包括样品特异性核苷酸标签和靶核苷酸序列。 在一些实施方案中，测定方法需要分别使S样品经受产生一组T标记的靶核苷酸序列的编码反应，每个标记的靶核苷酸序列包括与靶核苷酸序列连接的第一个核苷酸标签，其连接到第二个 核苷酸标签。 在任一情况下，可以将来自S样品的标记的靶核苷酸序列混合以形成测定混合物并随后测定。

    Method and program for performing baseline correction of amplification curves in a PCR experiment
    9.
    发明授权
    Method and program for performing baseline correction of amplification curves in a PCR experiment 有权
    用于在PCR实验中进行扩增曲线的基线校正的方法和程序

    公开(公告)号：US08473216B2

    公开(公告)日：2013-06-25

    申请号：US11948782

    申请日：2007-11-30

    申请人： Gang Sun ,  Ramesh Ramakrishnan ,  Robert C. Jones

    发明人： Gang Sun ,  Ramesh Ramakrishnan ,  Robert C. Jones

    IPC分类号： G01N33/48

    CPC分类号： C12Q1/686 ,  C12Q2565/629 ,  C12Q2565/501 ,  C12Q2545/114

    摘要： A method of adjusting amplification curves in a PCR experiment includes receiving a plurality of amplification curves for a sample and computing a first parameter for each of the plurality of amplification curves. The method also includes computing a second parameter for each of the plurality of amplification curves and computing a third parameter using at least a portion of the first or second parameters. The method further includes computing an offset for each of the plurality of amplification curves. The offset is a function of the first parameter and the third parameter. Moreover, the method includes adjusting at least one of the plurality of amplification curves by subtracting the offset.

    摘要翻译： 在PCR实验中调整扩增曲线的方法包括接收样品的多个放大曲线并计算多个放大曲线中的每一个的第一参数。 该方法还包括为多个放大曲线中的每一个计算第二参数，并使用第一或第二参数的至少一部分来计算第三参数。 该方法还包括计算多个放大曲线中的每一个的偏移量。 偏移量是第一个参数和第三个参数的函数。 此外，该方法包括通过减去偏移来调整多个放大曲线中的至少一个。