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公开(公告)号:US6013750A
公开(公告)日:2000-01-11
申请号:US649588
申请日:1996-07-09
IPC分类号: C08L75/16 , C08F290/00 , C08F290/06 , C08G18/67 , C08L55/00 , C09D4/02 , C09D11/00 , C09D11/02 , C09D11/10 , C09D155/00 , C09D175/14 , C09D175/16 , C09J4/00 , C09J4/02 , C09J5/00 , C09J155/00 , C09J175/16 , C09K3/10 , C08F126/02
CPC分类号: C09K3/1021 , C08F290/067 , C08G18/672 , C08L55/005 , C09D155/005 , C09J155/005 , C09J175/16
摘要: The invention pertains to an aerobically curing composition comprising free-radical polymerizable compounds, an activator system, and optionally conventional additives, characterized in that said composition contains as said free-radical polymerizable compounds at least one polyurethane (meth)acrylate one or more (meth)acrylate comonomers; wherein said composition contains from 20 to 80% by weight of said polyurethane (meth)acrylate and from 80 to 20% by weight of said (meth)acrylate comonomer(s), based on the total of polymerizable compounds, and said activator system is a hydrazone activator system, as well as the preparation and use of said composition.
摘要翻译: PCT No.PCT / EP94 / 03847 Sec。 371日期:1996年7月9日 102(e)日期1996年7月9日PCT 1994年11月22日PCT公布。 公开号WO95 / 14720 PCT 日期1995年6月1日本发明涉及包含自由基可聚合化合物,活化剂体系和任选常规添加剂的有氧固化组合物,其特征在于所述组合物含有作为所述自由基可聚合化合物的至少一种聚氨酯(甲基)丙烯酸酯 一种或多种(甲基)丙烯酸酯共聚单体; 其中所述组合物含有20-80重量%的所述聚氨酯(甲基)丙烯酸酯和80-20重量%的所述(甲基)丙烯酸酯共聚单体,基于可聚合化合物的总量,所述活化剂体系是 腙活化剂体系,以及所述组合物的制备和用途。
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公开(公告)号:US5962540A
公开(公告)日:1999-10-05
申请号:US776728
申请日:1997-03-03
申请人: Carsten Friese , Frank Bergmann , Thomas Huver
发明人: Carsten Friese , Frank Bergmann , Thomas Huver
IPC分类号: C09J9/00 , C08G18/67 , C08J9/10 , C08L75/16 , C09J4/00 , C09J4/02 , C09J5/08 , C09J11/06 , C09J133/04 , C09J175/00 , C09J175/16 , C09J201/00 , H01F27/32 , C08J9/02
CPC分类号: C08J9/102 , C08G18/672 , C09J175/16 , C09J4/00 , C09J5/08 , C08J2207/02 , C08J2375/16 , C08L75/16 , C09J2475/00
摘要: A reaction adhesive becomes porous after hardening. This allows both small and larger gaps to be sealed between the bonded substrates, as the liquid adhesive has a low to medium viscosity and the hardened glue shows a marked volume increase. Reaction adhesives based on (meth)acrylates and that contain azo-bis-isobutyronitrile as initiator and expanding agent are preferably used. The disclosed reaction adhesive is preferably used for gluing reactive coils.
摘要翻译: PCT No.PCT / EP95 / 02961 Sec。 371日期1997年3月3日 102(e)1997年3月3日PCT PCT 1995年7月25日PCT公布。 公开号WO96 / 04347 日期1996年2月15日硬化后反应粘合剂变多孔。 这允许在粘合的基底之间密封小的和较大的间隙,因为液体粘合剂具有低至中等的粘度,并且硬化的胶显示出显着的体积增加。 优选使用基于(甲基)丙烯酸酯并含有偶氮双异丁腈作为引发剂和发泡剂的反应粘合剂。 所公开的反应粘合剂优选用于胶合反应性线圈。
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公开(公告)号:US6096842A
公开(公告)日:2000-08-01
申请号:US875394
申请日:1997-07-24
申请人: Carsten Friese , Frank Bergmann
发明人: Carsten Friese , Frank Bergmann
摘要: Aerobically hardening adhesives based on polymers of acrylates or methacrylates having a relatively high boiling point such as polyurethane (meth)acrylates are described, wherein the adhesives contain at least one initiator capable of forming free radicals, at least one accelerator, and at least one siccative. Such adhesives are largely odorless and harden completely such that the adhesive surface at the boundary with air is not tacky.
摘要翻译: PCT No.PCT / EP96 / 00199 Sec。 371日期1997年7月24日 102(e)1997年7月24日PCT PCT 1996年1月18日PCT公布。 公开号WO96 / 23036 日本1996年8月1日描述了基于具有较高沸点的丙烯酸酯或甲基丙烯酸酯如聚氨酯(甲基)丙烯酸酯的聚合物的自动硬化粘合剂,其中粘合剂含有至少一种能形成自由基的引发剂,至少一种促进剂, 和至少一个siccative。 这种粘合剂大部分无臭并且完全硬化,使得与空气边界处的粘合剂表面不发粘。
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公开(公告)号:US08350038B2
公开(公告)日:2013-01-08
申请号:US12898107
申请日:2010-10-05
申请人: Rainer Beckert , Frank Bergmann , Dieter Heindl , Rupert Herrmann , Hans-Peter Josel , Thomas Welzel
发明人: Rainer Beckert , Frank Bergmann , Dieter Heindl , Rupert Herrmann , Hans-Peter Josel , Thomas Welzel
IPC分类号: C07D217/22 , C07D217/00
CPC分类号: C07D401/04
摘要: Disclosed are pyridinyl-isoquinoline-dione derivatives, methods of producing these derivatives, conjugates comprising the pyridinyl-isoquinoline dione derivatives and (i) a solid support, or (ii) a biomolecule, methods of producing these conjugates as well as the use of these conjugates as quenchers in fluorescence resonance energy transfer (FRET).
摘要翻译: 公开了吡啶基 - 异喹啉二酮衍生物,制备这些衍生物的方法,包含吡啶基 - 异喹啉二酮衍生物的共轭物和(i)固体支持物,或(ii)生物分子,制备这些缀合物的方法以及这些 共轭物作为荧光共振能量转移(FRET)中的猝灭剂。
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公开(公告)号:US20090208930A1
公开(公告)日:2009-08-20
申请号:US11658569
申请日:2005-09-02
CPC分类号: C12Q1/6848 , C12Q1/6858 , C12Q2523/125 , C12Q2521/331
摘要: The invention is related to a method for amplifying a methylated target nucleic acid in a sample while avoiding amplification of a non-methylated target nucleic acid by inactivating it. This is accomplished by a restriction enzyme digest after bisulfite treatment of the target nucleic acid. The invention is further related to the use of a restriction enzyme to avoid amplification of a non-methylated target nucleic acid while amplifying a methylated target nucleic acid in a sample and kits for performing the methods according to the invention.
摘要翻译: 本发明涉及一种用于扩增样品中的甲基化靶核酸的方法,同时避免通过使其失活来扩增非甲基化靶核酸。 这是通过目标核酸亚硫酸氢盐处理后的限制酶消化完成的。 本发明进一步涉及使用限制酶避免扩增未甲基化的靶核酸同时扩增样品中的甲基化靶核酸和用于实施根据本发明的方法的试剂盒。
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公开(公告)号:US20060058518A1
公开(公告)日:2006-03-16
申请号:US10540406
申请日:2004-01-28
CPC分类号: C07H21/04 , C01D5/145 , C12Q1/6827 , C12Q2527/101 , C12Q2523/125
摘要: The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines, whereby the nucleic acid is incubated in a solution comprising the nucleic acid for a time period of 1.5 to 3.5 hours at a temperature between 70 and 90° C., whereby the concentration of bisulfite in the solution is between 3 M and 6.25 M and whereby the pH value of the solution is between 5.0 and 6.0 whereby the nucleic acid, i.e. the cytosine bases in the nucleic acid, are deaminated. Then the solution comprising the deaminated nucleic acid is desulfonated and preferably desalted. The application is further related to a solution comprising bisulfite with a certain pH and uses thereof as well as a kit comprising the solution.
摘要翻译: 本申请涉及用于进行亚硫酸氢盐反应以确定核酸(即甲基化和非甲基化胞嘧啶)中的甲基化位置的方法,由此将核酸在包含核酸的溶液中温育1.5天至 在70〜90℃的温度下3.5小时,由此在该溶液中的亚硫酸氢盐浓度在3〜6.25M之间,由此溶液的pH值在5.0和6.0之间,由此核酸,即胞嘧啶碱基 在核酸中进行脱氨。 然后将包含脱氨酸核酸的溶液脱磺化并优选脱盐。 该应用还涉及包含具有一定pH的亚硫酸氢盐和其用途的溶液以及包含该溶液的试剂盒。
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公开(公告)号:US06235476B1
公开(公告)日:2001-05-22
申请号:US09242536
申请日:1999-03-17
申请人: Frank Bergmann , Rupert Herrmann , Uwe Kobold
发明人: Frank Bergmann , Rupert Herrmann , Uwe Kobold
IPC分类号: C12Q168
CPC分类号: C12Q1/6872 , C12Q1/6827 , Y10T436/143333
摘要: A method for detecting nucleic acids by binding a probe P to a partial sequence S contained in the nucleic acid to produce a binding product B1, degrading the nucleic acid to produce a binding product B2 containing a partial nucleic acid F of a defined length and detecting the binding product B2 or the partial nucleic acid F a part based on its mass is particularly suitable for the parallel detection of nucleic acid of different sequences.
摘要翻译: 通过将探针P与核酸中所含的部分序列S结合以产生结合产物B1来检测核酸的方法,使核酸降解以产生含有限定长度的部分核酸F的结合产物B2并检测 结合产物B2或部分核酸F基于其质量的部分特别适用于不同序列的核酸的平行检测。
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公开(公告)号:US08877464B2
公开(公告)日:2014-11-04
申请号:US13191700
申请日:2011-07-27
CPC分类号: C12Q1/686 , C12P19/34 , C12Q1/68 , C12Q1/6851 , G01N21/6428 , G01N2021/6432 , G01N2021/6441
摘要: The present invention relates to new methods and uses for the qualitative and quantitative detection of microbial nucleic acids using at least a first control nucleic acid, or a first and a second control nucleic acid in different concentrations. The method is based on amplification of nucleic acids, for example the polymerase chain reaction. Further provided are kits comprising components for performing said methods and uses.
摘要翻译: 本发明涉及使用至少第一对照核酸或不同浓度的第一和第二对照核酸定性和定量检测微生物核酸的新方法和用途。 该方法基于核酸的扩增,例如聚合酶链反应。 还提供了包括用于执行所述方法和用途的组件的套件。
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公开(公告)号:US08445206B2
公开(公告)日:2013-05-21
申请号:US13361549
申请日:2012-01-30
CPC分类号: C12Q1/6841 , C12Q1/6876 , C12Q1/6886 , C12Q2565/1025 , C12Q2563/107
摘要: The present disclosure relates to a set of at least 100 single-stranded oligonucleotide probes directed against (or complementary to) portions of a genomic target sequence of interest. The present disclosure also relates to a method of detecting a genomic target sequence of interest using the set of oligonucleotide probes and a method of generating the set of oligonucleotide probes. Further, the present disclosure relates to a kit comprising the set of oligonucleotide probes and at least one further component.
摘要翻译: 本公开内容涉及针对感兴趣的基因组靶序列的(或互补)部分的一组至少100个单链寡核苷酸探针。 本公开还涉及使用该组寡核苷酸探针检测感兴趣的基因组靶序列的方法和产生寡核苷酸探针组的方法。 此外,本公开涉及包含该组寡核苷酸探针和至少一种另外的组分的试剂盒。
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公开(公告)号:US08124338B2
公开(公告)日:2012-02-28
申请号:US12481807
申请日:2009-06-10
CPC分类号: C12N15/1006
摘要: The invention provides the use of tetraethylene glycol dimethyl ether for adsorbing nucleic acids to solid phases such as those with silica surfaces. To this end, the invention also provides compositions comprising TDE. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. Particularly, the invention encompasses methods for the purification of nucleic acids with low molecular weight. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.
摘要翻译: 本发明提供四乙二醇二甲醚用于将核酸吸附到固相上,例如具有二氧化硅表面的那些。 为此,本发明还提供了包含TDE的组合物。 方法被公开并要求从样品中纯化核酸,以及用于实施这些方法的试剂盒。 特别地,本发明包括用于纯化低分子量核酸的方法。 通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。
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