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34 条记录 (耗时 0.019 秒)

    Monoclonal Antibodies With Specificity For Fetal Erythroid Cells
    5.
    发明申请
    Monoclonal Antibodies With Specificity For Fetal Erythroid Cells 失效
    胎儿红细胞特异性单克隆抗体

    公开(公告)号：US20070276161A1

    公开(公告)日：2007-11-29

    申请号：US11661544

    申请日：2005-08-04

    申请人： Christine Hollmann ,  Silke Zimmermann ,  Stefan Stachelhaus ,  Winfried Albert

    发明人： Christine Hollmann ,  Silke Zimmermann ,  Stefan Stachelhaus ,  Winfried Albert

    IPC分类号： C07C213/10 ,  C07C215/02

    CPC分类号： C07C213/10 ,  C07C215/08

    摘要： Methods for improving color properties of triethanolamine, and triethanolamine compositions treated thereby, are disclosed, wherein the methods comprise: (a) providing a composition comprising triethanolamine; and (b) contacting the composition with an acid component and a basic component; wherein the acid component comprises an acid selected from the group consisting of phosphorous acid, hypophosphorous acid and mixtures thereof; and wherein the basic component comprises a compound selected from the group consisting of alkali metal hydroxides, alkaline earth metal hydroxides, ammonium hydroxides according to general formula (I), and mixtures thereof: [R1R2R3(2-hydroxyethyl)ammonium]hydroxide  (I) wherein R1, R2 and R3 each independently represents a C1-30 alkyl or a C2-10 hydroxyalkyl; with the proviso that where the basic component comprises an alkali metal hydroxide, the molar ratio of acid component:basic component is 1:0.1 to 1:1, and where the basic component comprises an alkaline earth metal hydroxide, the molar ratio of acid component:basic component is 1:0.05 to 1:0.5.

    摘要翻译： 公开了用于改善三乙醇胺的颜色特性和由此处理的三乙醇胺组合物的方法，其中所述方法包括：（a）提供包含三乙醇胺的组合物; 和（b）使组合物与酸组分和碱性组分接触; 其中所述酸组分包含选自亚磷酸，次磷酸及其混合物的酸; 并且其中所述碱性组分包括选自碱金属氢氧化物，碱土金属氢氧化物，根据通式（I）的氢氧化铵的化合物及其混合物：<？在线公式描述=“在线 公式“end =”lead“？> [R 1] R 2 R 3（2-羟乙基）铵]氢氧化物（I） in-line-formula description =“In-line Formulas”end =“tail”？>其中R 1，R 2和R 3， 各自独立地表示C 1〜30的烷基或C 2〜10 N个羟基烷基; 条件是碱性成分含有碱金属氢氧化物时，酸成分：碱成分的摩尔比为1:0.1〜1:1，碱性成分含有碱土金属氢氧化物时，酸成分 基本成分为1:0.05〜1:0.5。

    Reagency and Method for Preventing Time-Dependent Expression in Biological Cells
    6.
    发明申请
    Reagency and Method for Preventing Time-Dependent Expression in Biological Cells 审中-公开
    用于预防生物细胞中时间依赖性表达的试剂和方法

    公开(公告)号：US20080206866A1

    公开(公告)日：2008-08-28

    申请号：US11909142

    申请日：2006-03-22

    申请人： Veit Zieglschmid ,  Oliver Bocher ,  Winfried Albert

    发明人： Veit Zieglschmid ,  Oliver Bocher ,  Winfried Albert

    IPC分类号： C12N5/06

    CPC分类号： C12Q1/6806 ,  C12Q2600/158 ,  C12Q2527/127

    摘要： The present invention relates to a reagent for prevention of time-dependent, induced expression in biological cells in a sample. It likewise relates to a method for this purpose and also to cell preparations treated in this manner, the reagent for prevention of time-dependence induced expression in biological cells in a sample ex vivo containing a formaldehyde donor.

    摘要翻译： 本发明涉及用于预防样品中生物细胞中时间依赖性诱导表达的试剂。 它同样涉及用于此目的的方法以及以这种方式处理的细胞制剂，用于预防离体含有甲醛供体的样品中生物细胞中时间依赖性诱导表达的试剂。

    Process for the quantification of cell populations or subpopulations and a reagent suitable therefor
    8.
    发明授权
    Process for the quantification of cell populations or subpopulations and a reagent suitable therefor 失效
    用于定量细胞群或亚群的方法和适合其的试剂

    公开(公告)号：US4876189A

    公开(公告)日：1989-10-24

    申请号：US927803

    申请日：1986-11-05

    申请人： Hartmut Schetters ,  Josef Endl ,  Winfried Albert

    发明人： Hartmut Schetters ,  Josef Endl ,  Winfried Albert

    IPC分类号： G01N33/53 ,  C12Q1/28 ,  C12Q1/34 ,  G01N33/536 ,  G01N33/543 ,  G01N33/569

    CPC分类号： G01N33/56972 ,  G01N33/56966 ,  Y10S435/81 ,  Y10S435/967 ,  Y10T436/101666

    摘要： A process for quantification of cell populations or subpopulations, by incubating a sample with labelled antibodies directed against characteristic surface antigens of the cell population to be quantified to form labelled antibody/antigen complexes. Standards with known, differing particle concentration and having comparable sedimentation behavior to the cells to be determined and further, carrying molecules which are directed against the labelled antibody or a part hereof are also incubated with the labelled antibodies. The cells of the sample solution, as well as the particles of the standard solution, are separated off from the excess labelled antibodies and the amount of the labelling is measured not only on the cells but also on the particles. By comparison of the measurement value from the sample with the measurement values from the standard solutions, there is ascertained the number of cells to be determined in the sample. The standard and a reagent kit for carrying out the process are also the subject of this application.

    摘要翻译： 通过将样品与针对要定量的细胞群的特征性表面抗原的标记抗体孵育以形成标记的抗体/抗原复合物，来定量细胞群体或亚群体的方法。 具有已知的，不同的颗粒浓度并且具有与要测定的细胞具有可比较的沉降行为的标准品，并且进一步携带针对标记的抗体或其部分的分子也与标记的抗体一起温育。 将样品溶液的细胞以及标准溶液的颗粒与过量标记的抗体分离出来，并且不仅在细胞上而且在颗粒上测量标记量。 通过将来自样品的测量值与来自标准溶液的测量值的比较，确定样品中待确定的细胞数量。 用于执行该过程的标准品和试剂盒也是本申请的主题。

    Process for the preparation of immunologically active enzyme tagged conjugates
    9.
    发明授权
    Process for the preparation of immunologically active enzyme tagged conjugates 失效
    用于制备免疫活性酶标记的缀合物的方法

    公开(公告)号：US4486534A

    公开(公告)日：1984-12-04

    申请号：US379794

    申请日：1982-05-19

    申请人： Winfried Albert ,  Helmut Lenz

    发明人： Winfried Albert ,  Helmut Lenz

    IPC分类号： C12N9/96 ,  C12Q1/00 ,  G01N33/535 ,  G01N33/54

    CPC分类号： C12N9/96 ,  G01N33/535 ,  Y10S435/961

    摘要： A process for the preparation of immunologically active tagged conjugates by covalent linking of an enzyme with an antigen or haptene, which process comprises placing a solution of a mixture of immunologically active and inactive conjugates in contact with an insoluble complex former able to form a non-immunological complex reversibly with the untagged antigen or haptene component of the conjugate, separating the insoluble complex former, and eluting with a desorbent for untagged antigen or haptene.

    摘要翻译： 通过酶与抗原或半抗原共价连接来制备免疫活性标记的缀合物的方法，该方法包括将免疫活性和非活性轭合物的混合物的溶液与能够形成非 - 免疫复合物与缀合物的未标记抗原或半抗原组分可逆地分离，分离不溶性络合物，并用用于未标记的抗原或半抗原的解吸剂洗脱。

    Solid Phase Cell Isolation and/or Enrichment Method
    10.
    发明申请
    Solid Phase Cell Isolation and/or Enrichment Method 有权
    固相细胞分离和/或富集方法

    公开(公告)号：US20100285581A1

    公开(公告)日：2010-11-11

    申请号：US12678636

    申请日：2008-09-17

    申请人： Siegfried Hauch ,  Winfried Albert

    发明人： Siegfried Hauch ,  Winfried Albert

    IPC分类号： C12N5/071 ,  C12N5/078 ,  C12N5/09 ,  C12N5/073

    CPC分类号： G01N33/574 ,  C12N2500/35 ,  G01N1/34 ,  G01N33/54393

    摘要： The present invention concerns a solid phase method for isolating and/or enriching predetermined cells from a sample. Such methods are used e.g. to isolate and enrich predetermined cells like fetal cells from a sample of maternal peripheral blood, tumor cells from a sample of body fluid or stem cells from a fluid or fluidized sample of body tissue or body fluid. The solid phase isolation method of the present invention is used for isolating predetermined cells from a sample containing such predetermined cells by binding the predetermined cells to a solid surface. According to the invention the sample is contacted with the solid surface and then removed from the solid surface, wherein the sample or a washing buffer contains a polyol during or after contacting the sample with the solid surface.

    摘要翻译： 本发明涉及用于从样品中分离和/或富集预定细胞的固相方法。 这样的方法例如使用。 从母体外周血样品，体液样品或来自体液或体液流体样本的干细胞的肿瘤细胞中分离和富集预定细胞，如胎儿细胞。 本发明的固相分离方法用于通过将预定细胞与固体表面结合来从含有这种预定细胞的样品中分离出预定的细胞。 根据本发明，样品与固体表面接触，然后从固体表面除去，其中样品或洗涤缓冲液在与样品与固体表面接触期间或之后含有多元醇。