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    Process and reagent for the determination of creatinine
    1.
    发明授权
    Process and reagent for the determination of creatinine 失效
    用于测定肌酐的方法和试剂

    公开(公告)号：US4543325A

    公开(公告)日：1985-09-24

    申请号：US449925

    申请日：1982-12-15

    申请人： Winfried Albert ,  Joachim Ziegenhorn ,  Joachim Siedel ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz

    发明人： Winfried Albert ,  Joachim Ziegenhorn ,  Joachim Siedel ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz

    IPC分类号： G01N33/53 ,  G01N33/70 ,  C12N9/96 ,  C12Q1/34

    CPC分类号： G01N33/70 ,  Y10S435/81 ,  Y10S435/961 ,  Y10S436/822

    摘要： The present invention provides a process for the immunological determination of creatinine, wherein creatinine is converted into 1-methylhydantoin, the 1-methylhydantoin formed is incubated in an aqueous medium with antibodies which are directed against a conjugate of a first hydantoin derivative of the general formula: ##STR1## in which R.sup.1, R.sup.2, R.sup.3 and R.sup.4, which can be the same or different, are hydrogen atoms, alkyl radicals containing up to 3 carbon atoms or phenyl radicals, with a first hapten carrier substance suitable for antibody formation, reacted with a conjugate of a second hydantoin derivative of general formula (I) with a second hapten carrier substance, one of the components antibody and conjugate being present in the solid phase or in dissolved form and the other component being present in dissolved form, and the inhibition of the binding reaction between the antibodies and the hydantoin conjugate with the second hapten carrier substance is measured.The present invention also provides a reagent for the immunological determination of creatinine, wherein it contains creatinine iminohydrolase, antibodies against a conjugate of a hydantoin of the general formula: ##STR2## in which R.sup.1, R.sup.2, R.sup.3 and R.sup.4, which can be the same or different, are hydrogen atoms, alkyl radicals containing up to 3 carbon atoms or phenyl radicals, with a first hapten carrier substance, a conjugate of a hydantoin of general formula (I) with a second hapten carrier substance which does not cross-react substantially with the first hapten carrier substance and a buffer substance.

    摘要翻译： 本发明提供了一种免疫学测定肌酐的方法，其中将肌酐转化为1-甲基乙内酰脲，所形成的1-甲基乙内酰脲在水性介质中与针对通式 ：其中可以相同或不同的R 1，R 2，R 3和R 4为氢原子，含有至多3个碳原子的烷基或苯基，其中第一半抗原载体物质适用于抗体 与通式（I）的第二乙内酰脲衍生物与第二半抗原载体物质的缀合物反应，抗体和缀合物之一是固相或溶解形式存在，另一种成分以溶解形式存在 并且测量了与第二半抗原载体物质的抗体和乙内酰脲缀合物之间的结合反应的抑制。 本发明还提供了一种用于免疫测定肌酐的试剂，其中含有肌酐亚氨基水解酶，针对通式如下的乙内酰脲的缀合物的抗体：其中R1，R2，R3和R4可以相同 或不同的是氢原子，含有至多3个碳原子的烷基或苯基，具有第一半抗原载体物质，通式（I）的乙内酰脲与不会基本上交叉反应的第二半抗原载体物质的缀合物 具有第一半抗原载体物质和缓冲物质。

    Creatinine specific antibody
    2.
    发明授权
    Creatinine specific antibody 失效
    肌酸酐特异性抗体

    公开(公告)号：US4485177A

    公开(公告)日：1984-11-27

    申请号：US412411

    申请日：1982-08-27

    申请人： Joachim Siedel ,  Ulrich Neumann ,  Joachim Ziegenhorn ,  Hans-George Batz ,  Helmut Lenz ,  Brigitte Pautz ,  Winfried Albert

    发明人： Joachim Siedel ,  Ulrich Neumann ,  Joachim Ziegenhorn ,  Hans-George Batz ,  Helmut Lenz ,  Brigitte Pautz ,  Winfried Albert

    IPC分类号： G01N33/53 ,  A61K38/00 ,  C07K16/18 ,  G01N33/531 ,  G01N33/543 ,  G01N33/577 ,  G01N33/70 ,  G01N33/54 ,  A61K39/00 ,  C07G7/00

    CPC分类号： C07K16/18 ,  G01N33/531 ,  G01N33/54306 ,  G01N33/577 ,  G01N33/70 ,  A61K38/00

    摘要： The present invention provides a creatinine antibody and a process for the preparation thereof, wherein a conjugate of creatinine and a material suitable for antiserum formation, which are connected via an aliphatic or araliphatic carboxylic acid as bridge member, is used as immunogen for antiserum formation.The present invention is also concerned with the use of the above antibody for the immunological determination of creatinine, wherein the antibody is incubated with a creatinine-containing sample solution, reacted with a conjugate of creatinine with a hapten carrier substance, whereby one of the components, antibody and conjugate, is present in the solid phase or in dissolved form and the other component is present in dissolved form and the inhibition of the binding reaction between the antibody and the creatinine conjugate is measured.Furthermore, the present invention provides a reagent for the immunological determination of creatinine, wherein it contains the above antibody, a conjugate of creatinine with a hapten carrier substance and buffer substance.

    摘要翻译： 本发明提供一种肌酸酐抗体及其制备方法，其中通过脂肪族或脂环族羧酸作为桥接剂连接肌酸酐和适于抗血清形成的物质的缀合物，用作抗血清形成的免疫原。 本发明还涉及上述抗体用于肌酸酐免疫学测定的用途，其中将抗体与含肌酸酐的样品溶液一起温育，与肌酸酐的缀合物与半抗原载体物质反应，由此其中一种成分 ，抗体和缀合物以固相或溶解形式存在，另一组分以溶解形式存在，并测量抗体与肌酐结合物之间结合反应的抑制。 此外，本发明提供了用于肌酸酐免疫测定的试剂，其中含有上述抗体，肌酸酐与半抗原载体物质和缓冲物质的结合物。

    Creatinine antibody
    4.
    发明授权
    Creatinine antibody 失效
    肌酸酐抗体

    公开(公告)号：US4578361A

    公开(公告)日：1986-03-25

    申请号：US656957

    申请日：1984-10-02

    申请人： Joachim Siedel ,  Ulrich Neumann ,  Joachim Ziegenhorn ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz ,  Winfried Albert

    发明人： Joachim Siedel ,  Ulrich Neumann ,  Joachim Ziegenhorn ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz ,  Winfried Albert

    IPC分类号： G01N33/53 ,  A61K38/00 ,  C07K16/18 ,  G01N33/531 ,  G01N33/543 ,  G01N33/577 ,  G01N33/70 ,  A61K39/00

    CPC分类号： C07K16/18 ,  G01N33/531 ,  G01N33/54306 ,  G01N33/577 ,  G01N33/70 ,  A61K38/00

    摘要： The present invention provides a creatinine antibody and a process for the preparation thereof, wherein a conjugate of creatinine and a material suitable for antiserum formation, which are connected via an aliphatic or araliphatic carboxylic acid as bridge member, is used as immunogen for antiserum formation.The present invention is also concerned with the use of the above antibody for the immunological determination of creatinine, wherein the antibody is incubated with a creatinine-containing sample solution, reacted with a conjugate of creatinine with a hapten carrier substance, whereby one of the components, antibody and conjugate, is present in the solid phase or in dissolved form and the other component is present in dissolved form and the inhibition of the binding reaction between the antibody and the creatinine conjugate is measured.Furthermore, the present invention provides a reagent for the immunological determination of creatinine, wherein it contains the above antibody, a conjugate of creatinine with a hapten carrier substance and buffer substance.

    摘要翻译： 本发明提供一种肌酸酐抗体及其制备方法，其中通过脂肪族或脂环族羧酸作为桥接剂连接肌酸酐和适于抗血清形成的物质的缀合物，用作抗血清形成的免疫原。 本发明还涉及上述抗体用于肌酸酐免疫学测定的用途，其中将抗体与含肌酸酐的样品溶液一起温育，与肌酸酐的缀合物与半抗原载体物质反应，由此其中一种成分 ，抗体和缀合物以固相或溶解形式存在，另一组分以溶解形式存在，并测量抗体与肌酐结合物之间结合反应的抑制。 此外，本发明提供了用于肌酸酐免疫测定的试剂，其中含有上述抗体，肌酸酐与半抗原载体物质和缓冲物质的结合物。

    Process and reagent for specific determination of pancreatic alpha amylase
    5.
    发明授权
    Process and reagent for specific determination of pancreatic alpha amylase 失效
    用于特异性测定胰腺α淀粉酶的方法和试剂

    公开(公告)号：US5573918A

    公开(公告)日：1996-11-12

    申请号：US762646

    申请日：1991-09-19

    申请人： Helmut Lenz ,  Martin Gerber ,  Winfried Albert

    发明人： Helmut Lenz ,  Martin Gerber ,  Winfried Albert

    IPC分类号： G01N33/573 ,  A61K39/395 ,  C07K14/005 ,  C07K14/195 ,  C07K16/00 ,  C07K16/40 ,  C12N15/00 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/40 ,  C12Q1/48 ,  G01N33/577

    CPC分类号： C12Q1/40 ,  C07K16/40 ,  Y10S435/81

    摘要： The present invention provides a process for the specific determination of pancreatic alpha-amylase in the presence of salivary alpha-amylase in body fluids, especially in serum, plasma, duodenal juice or urine, by reaction with a system for the detection of alpha-amylase in the presence of a monoclonal antibody which inhibits salivary alpha-amylase wherein, as inhibitor, there is used a first monoclonal antibody which specifically inhibits the salivary enzyme by less than 97%, in combination with a second monoclonal anti-saliva alpha-amylase antibody which inhibits this enzyme by less than 10%.The present invention also provides a reagent for the specific determination of pancreatic alpha-amylase in the presence of salivary alpha-amylase in body fluids, especially in serum, duodenal juice, plasma or urine, containing a system for the detection of alpha-amylase and a monoclonal antibody against salivary alpha-amylase wherein it contains a first monoclonal antibody which specifically inhibits the salivary enzyme by less than 97%, in combination with a second monoclonal anti-salivary alpha-amylase antibody which inhibits this enzyme by less than 10%.

    摘要翻译： 本发明提供了通过与用于检测α-淀粉酶的系统的反应，在体液中，特别是在血清，血浆，十二指肠汁或尿中的唾液α-淀粉酶存在下，特异性测定胰腺α-淀粉酶的方法 在抑制唾液α-淀粉酶的单克隆抗体的存在下，其中作为抑制剂，使用与第二单克隆抗唾液α-淀粉酶抗体组合的特异性抑制唾液酶的第一单克隆抗体小于97％ 其抑制该酶低于10％。 本发明还提供了用于在体液（特别是血清，十二指肠液，血浆或尿液）中存在唾液α-淀粉酶的情况下特异性测定胰腺α-淀粉酶的试剂，其含有用于检测α-淀粉酶的系统，以及 抗唾液α-淀粉酶的单克隆抗体，其中含有第一单克隆抗体，其特异性抑制唾液酶小于97％，与第二种单克隆抗唾液α-淀粉酶抗体组合，该抗体抑制该酶低于10％。

    Process for the preparation of immunologically active enzyme tagged conjugates
    7.
    发明授权
    Process for the preparation of immunologically active enzyme tagged conjugates 失效
    用于制备免疫活性酶标记的缀合物的方法

    公开(公告)号：US4486534A

    公开(公告)日：1984-12-04

    申请号：US379794

    申请日：1982-05-19

    申请人： Winfried Albert ,  Helmut Lenz

    发明人： Winfried Albert ,  Helmut Lenz

    IPC分类号： C12N9/96 ,  C12Q1/00 ,  G01N33/535 ,  G01N33/54

    CPC分类号： C12N9/96 ,  G01N33/535 ,  Y10S435/961

    摘要： A process for the preparation of immunologically active tagged conjugates by covalent linking of an enzyme with an antigen or haptene, which process comprises placing a solution of a mixture of immunologically active and inactive conjugates in contact with an insoluble complex former able to form a non-immunological complex reversibly with the untagged antigen or haptene component of the conjugate, separating the insoluble complex former, and eluting with a desorbent for untagged antigen or haptene.

    摘要翻译： 通过酶与抗原或半抗原共价连接来制备免疫活性标记的缀合物的方法，该方法包括将免疫活性和非活性轭合物的混合物的溶液与能够形成非 - 免疫复合物与缀合物的未标记抗原或半抗原组分可逆地分离，分离不溶性络合物，并用用于未标记的抗原或半抗原的解吸剂洗脱。

    Immune complex-specific antibodies for increased sensitivity in immunoassay array tests
    10.
    发明授权
    Immune complex-specific antibodies for increased sensitivity in immunoassay array tests 有权
    免疫复合物特异性抗体，用于在免疫测定阵列测试中提高灵敏度

    公开(公告)号：US08664007B2

    公开(公告)日：2014-03-04

    申请号：US11260938

    申请日：2005-10-28

    申请人： Ursula Klause ,  Helmut Lenz ,  Christine Markert-Hahn

    发明人： Ursula Klause ,  Helmut Lenz ,  Christine Markert-Hahn

    IPC分类号： G01N33/543

    CPC分类号： G01N33/543 ,  G01N33/6854

    摘要： The invention concerns a method for determining antigen-specific antibodies of a particular immunoglobulin class in a sample by means of an immunoassay in an array format in which various binding partners Bnx are bound on different discrete areas on a support where Bnx in each case contain the various antigens that are able to specifically bind to the antibodies to be detected, by incubating the support with the sample and a binding partner B2 which carries a label and subsequently detecting the label on the respective discrete areas wherein B2 specifically binds antibodies of a certain immunoglobulin class that have been bound in an antigen-specific manner.

    摘要翻译： 本发明涉及通过以阵列格式的免疫测定来测定样品中特定免疫球蛋白类别的抗原特异性抗体的方法，其中各种结合配偶体Bnx结合在支架上的不同离散区域上，其中每种情况下Bnx包含 能够通过将载体与样品一起孵育的各种抗原和携带标记的结合伴侣B2，随后检测各个离散区域上的标记，其中B2特异性结合某种免疫球蛋白的抗体 已经以抗原特异性方式结合的类。