公开(公告)号：US08323985B2

公开(公告)日：2012-12-04

申请号：US13269449

申请日：2011-10-07

申请人： Achim Wixforth ,  Christoph Gauer

发明人： Achim Wixforth ,  Christoph Gauer

IPC分类号： G01N1/10

CPC分类号： G01N29/222 ,  B01F11/0266 ,  B01F11/0283 ,  B01F13/0059 ,  B01F15/0201 ,  B01F15/0263 ,  B01F2215/0037 ,  B01L3/502792 ,  B01L2300/0816 ,  B01L2300/089 ,  B01L2400/0433 ,  B01L2400/0436 ,  B01L2400/0496 ,  G01N1/40 ,  Y10T436/25 ,  Y10T436/2575

摘要： The invention relates to a mixing method for mixing at least one small quantity of liquid, in which a quantity of liquid is applied in a reaction region and at least one surface sound wave is reacted with the quantity of liquid. The invention relates further to a mixing device for mixing at least one quantity of liquid for performing the method of the present invention, a use of the device, and a method of analysis for bond strengths on surfaces.

摘要翻译： 本发明涉及一种用于混合至少一种少量液体的混合方法，其中在反应区域中施加一定量的液体，并且至少一个表面声波与液体量反应。 本发明还涉及用于混合至少一种量的用于实施本发明的方法的液体的混合装置，该装置的使用以及对表面上的结合强度进行分析的方法。

公开(公告)号：US07459304B2

公开(公告)日：2008-12-02

申请号：US10470787

申请日：2001-12-19

申请人： Christoph Gauer

发明人： Christoph Gauer

IPC分类号： C12M1/34 ,  C12Q1/68 ,  G01N33/53 ,  C07C1/00 ,  C07H21/02 ,  C07K1/00

CPC分类号： B82Y30/00 ,  B01F11/02 ,  B01J19/0046 ,  B01J2219/00533 ,  B01J2219/00574 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00637 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  G01N2291/102 ,  C12Q2565/513

摘要： The invention relates to a method for the analysis of macromolecules using a microarray on which a plurality of first at least partly different macromolecules are located in a known arrangement, wherein the microarray is arranged on a solid surface on which a region is defined whose wetting properties differ from the surrounding solid surface such that a liquid with a plurality of second macromolecules preferably stays thereon, the liquid with the second macromolecules is brought onto the solid surface and at least partly removed and the second macromolecules remaining after the removal process are detected in order to determine from the position which of the first macromolecules have formed a bond with second macromolecules. The invention further relates to a method wherein a surface wave is launched to the liquid to distribute and/or thoroughly mix the liquid on the microarray.

摘要翻译： 本发明涉及使用微阵列分析大分子的方法，其中多个第一至少部分不同的大分子位于已知的布置中，其中微阵列布置在固定表面上，在该固体表面上定义了其湿润性质 与周围的固体表面不同，使得具有多个第二大分子的液体优选保留在其上，具有第二大分子的液体被带到固体表面上并且至少部分地除去，并且按照顺序检测在除去过程之后残留的第二大分子 从该位置确定哪个第一大分子与第二大分子形成键。 本发明还涉及一种方法，其中将表面波发射到液体以将液体分布和/或充分混合在微阵列上。

公开(公告)号：US20080193927A1

公开(公告)日：2008-08-14

申请号：US11631986

申请日：2005-07-27

申请人： Wofgang Mann ,  Christoph Gauer

发明人： Wofgang Mann ,  Christoph Gauer

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： C12Q1/6827 ,  C12Q1/6851 ,  C12Q2545/114 ,  C12Q2537/143

摘要： The invention relates to a method for determining the abundance of a given sequence or several sequences identical or nearly identical to the given sequence in a sample. The method comprises the following steps: carrying out one or more amplification reactions by means of which several different sections of the sequence or sequences of the sample can be amplified to give an amplified product, detection of whether given different sections of the sequence in the sample have been amplified and determination of the number of the sequence(s) in the sample by means of the abundance of the presence or otherwise of the given different sections in the amplified product.

摘要翻译： 本发明涉及一种用于确定给定序列的丰度或与样品中给定序列相同或几乎相同的几个序列的方法。 该方法包括以下步骤：进行一个或多个扩增反应，通过其可以扩增样品的序列或序列的几个不同部分以产生扩增产物，检测样品中是否给予序列的不同切片 已经通过扩增产物中给定的不同切片的存在或其它的丰度来扩增和确定样品中序列的数目。

公开(公告)号：US20110045595A1

公开(公告)日：2011-02-24

申请号：US11794768

申请日：2005-12-16

申请人： Christoph Gauer

发明人： Christoph Gauer

IPC分类号： G01N33/00

CPC分类号： B01L3/5027 ,  B01F11/0266 ,  B01F13/0059 ,  B01F15/0232 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/0605 ,  B01L2200/0621 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0433 ,  B01L2400/0677 ,  B01L2400/0688 ,  B01L2400/0694 ,  Y10T436/10 ,  Y10T436/25 ,  Y10T436/2575

摘要： A method or device for integrated dosing and intermixing of small amounts of liquid, has at least one dosing reservoir (3) connected to a reaction reservoir (1) via at least one joining structure (11) and entirely filled with a first liquid. The at least one joining structure (11) is preferably dimensioned such that surface tension of the first liquid prevents the first liquid from penetrating into the reaction reservoir (1) which is entirely filled with a second liquid contacting the first liquid on the joining structure (11). A flow pattern is created in or on the reaction reservoir (1) to thoroughly mix the two liquids.

公开(公告)号：US20100055679A1

公开(公告)日：2010-03-04

申请号：US11992428

申请日：2006-08-07

申请人： Christoph Gauer ,  Wolfgang Mann

发明人： Christoph Gauer ,  Wolfgang Mann

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6851 ,  C12Q2545/113 ,  C12Q2527/143 ,  C12Q2545/101

摘要： The invention relates to a method for the quantitative analysis of the number of a pre-determined sequence, and optionally of sequences homologous to the pre-determined sequence, in a biological sample, whereby a defined quantity of a biological sample is subjected to at least one amplification reaction which is adapted in such a way as to amplify at least two non-homologous sequences contained in the pre-determined sequence. The number of different amplification products obtained is then determined and compared with a frequency distribution. The invention further relates to a kit for the quantitative analysis of the number of a pre-determined sequence in a biological sample, and a device which is especially suitable for carrying out the inventive method.

摘要翻译： 本发明涉及一种用于在生物样品中定量分析预定序列数量和任选地与预定序列同源的序列的方法，由此定义量的生物样品经受至少 一个扩增反应，其以这样的方式进行调整，以扩增包含在预定序列中的至少两个非同源序列。 然后确定获得的不同扩增产物的数量并将其与频率分布进行比较。 本发明还涉及用于定量分析生物样品中预定序列数目的试剂盒，以及特别适用于实施本发明方法的装置。

公开(公告)号：US20080260582A1

公开(公告)日：2008-10-23

申请号：US11665877

申请日：2005-10-20

申请人： Christoph Gauer ,  Zeno von Guttenberg

发明人： Christoph Gauer ,  Zeno von Guttenberg

IPC分类号： B01J19/00 ,  F17D1/16 ,  G10K15/04

CPC分类号： F04B19/006 ,  B01F11/0258 ,  B01F13/0059 ,  B01L3/50273 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2400/0436 ,  B01L2400/088 ,  F04F7/00 ,  Y10T137/0391

摘要： A method is provided for displacing small amounts of fluids in micro channels, in which an amount of fluid is introduced into a channel system which has at least one area which corresponds in a topological manner to a ring, such that a closed path of the fluid is possible, and acoustic waves, which have at least one asymmetrical component on the plane of the channel system, are radiated into the fluid, this component defining the direction of displacement of the fluid. A micro channel system for carrying out the method is also provided.

摘要翻译： 提供了一种用于移动微通道中的少量流体的方法，其中将一定量的流体引入通道系统中，该通道系统具有至少一个以拓扑方式对应于环的区域，使得流体的封闭路径 是可能的，并且在通道系统的平面上具有至少一个不对称分量的声波被辐射到流体中，该部件限定了流体的位移方向。 还提供了一种用于执行该方法的微通道系统。

公开(公告)号：US08186869B2

公开(公告)日：2012-05-29

申请号：US11794770

申请日：2005-12-16

申请人： Christoph Gauer

发明人： Christoph Gauer

IPC分类号： B01F11/00

CPC分类号： B01L3/50273 ,  B01F11/0266 ,  B01F11/0283 ,  B01F13/0059 ,  B01L3/502776 ,  B01L2300/089 ,  B01L2400/0436 ,  B01L2400/0496 ,  B01L2400/0688

摘要： A method or device for integrated dosing and intermixing of small amounts of liquid, has a first liquid conveyed into or onto a first reservoir (3). A second reservoir (1) is entirely filled with a second liquid. The first and second liquids are brought into contact with each other via at least one joining duct structure (5) which has at least one area provided with a smaller cross section than the reservoirs (1,3) in the viewing direction of the connecting line between the two reservoirs (1,3). A laminar flow pattern is created along at least one portion of the joining duct structure (5), with the liquids thoroughly mixed in the second reservoir (1).

摘要翻译： 一种用于一体化计量和少量液体的混合的方法或装置，具有输送到第一储存器（3）中或其上的第一液体。 第二储存器（1）完全填充有第二液体。 第一和第二液体经由至少一个连接管道结构（5）彼此接触，所述至少一个接合管道结构（5）在连接线的观察方向上具有设置有比储存器（1,3）更小的横截面的至少一个区域 两个水库之间（1,3）。 沿着连接管道结构（5）的至少一部分产生层流图案，其中液体在第二储存器（1）中充分混合。

公开(公告)号：US20110081684A1

公开(公告)日：2011-04-07

申请号：US12312906

申请日：2007-09-18

申请人： Christoph Gauer ,  Wolfgang Mann ,  Marianna Alunni-Fabbroni

发明人： Christoph Gauer ,  Wolfgang Mann ,  Marianna Alunni-Fabbroni

IPC分类号： C12P19/34 ,  C12M1/34

CPC分类号： C12P7/10 ,  C12Q1/686 ,  Y02E50/16 ,  C12Q2565/629

摘要： The invention relates to a method for carrying out an enzymatic reaction, in particular for carrying out a polymerase chain reaction (PCR). Said method consists of the following steps: at least one eukaryotic cell is removed from a starting material; the cell core or cores of the eukaryotic cell(s) is/are coloured; at least one eukaryotic cell is deposited on a reaction point of a solid substrate in a liquid volume of less than 10 μl; it is detected whether at least one coloured cell core is present on a reaction point of the substrate, subsequently, an enzyme and optionally a reaction buffer is added to the eukaryotic cell(s) and the enzymatic reaction is subsequently started. Preferably, the claimed invention is carried out using a flow cytometer.

摘要翻译： 本发明涉及进行酶反应的方法，特别是用于进行聚合酶链式反应（PCR）。 所述方法包括以下步骤：从原料中除去至少一种真核细胞; 真核细胞的细胞核或核心被着色; 至少一个真核细胞以小于10μl的液体体积沉积在固体基质的反应点上; 检测在底物的反应点上是否存在至少一个有色细胞核，随后将酶和任选的反应缓冲液加入到真核细胞中，随后开始酶促反应。 优选地，所要求保护的发明使用流式细胞仪进行。

公开(公告)号：US20100184024A1

公开(公告)日：2010-07-22

申请号：US12225774

申请日：2007-02-15

申请人： Christoph Gauer ,  Wolfgang Mann

发明人： Christoph Gauer ,  Wolfgang Mann

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6837 ,  C12Q2565/507 ,  C12Q2545/114 ,  C12Q2565/518 ,  C12Q2565/513

摘要： A method is provided for characterizing a mixed sample having at least two particles with nucleic acids from different individuals, where each particle has nucleic acid from one or more individuals, in particular for the quantitative determination of the absolute and/or relative copy number of a predetermined sequence of an individual, of which nucleic acid is present in the mixed sample, having the steps: a) isolating the particles and applying at least two individual particles to a substrate, where each of the at least two particles is deposited in each case individually to a hydrophilic reaction site, surrounded by a hydrophobic zone, of the substrate in a volume of less than 10 μl so that precisely one particle is present per reaction site, b) analysis of at least two of the particles deposited to the substrate at the reaction site of the substrate to assign each of the particles to individuals from the mixed sample by genotyping, where at least 80% of the particles analysed are to be assigned to an individual, and c) further characterization of the analysed particles. Moreover, a kit which is suitable in particular for carrying out this method, is also provided.

摘要翻译： 提供了一种用于表征具有至少两个颗粒的混合样品的方法，所述混合样品具有来自不同个体的核酸，其中每个颗粒具有来自一个或多个个体的核酸，特别是用于定量确定不同个体的绝对和/或相对拷贝数 个体的预定序列，其中核酸存在于混合样品中，具有以下步骤：a）分离颗粒并将至少两个单独的颗粒施加至基底，其中每个至少两个颗粒在每种情况下沉积 分别以小于10μl的体积由疏水区包围的亲水反应位点，使得每个反应位点存在精确的一个颗粒，b）分析至少两个沉积到基材上的颗粒 底物的反应位点通过基因分型将每个颗粒分配给来自混合样品的个体，其中至少80％的分析的颗粒是t o分配给个人，和c）进一步表征分析的粒子。 此外，还提供了特别适于实施该方法的试剂盒。

公开(公告)号：US20060035369A1

公开(公告)日：2006-02-16

申请号：US11197142

申请日：2005-08-04

申请人： Christoph Gauer ,  Jurgen Scriba ,  Joachim Arzt ,  Roland Kirchner ,  Andreas Togl

发明人： Christoph Gauer ,  Jurgen Scriba ,  Joachim Arzt ,  Roland Kirchner ,  Andreas Togl

IPC分类号： C12M1/34

CPC分类号： B01L13/02 ,  B01L3/5021 ,  B01L2300/0822

摘要： The invention relates to a sample support washing container for simultaneous washing of at least two sample slides, with a filling opening, through which the sample slides can be introduced, a liquid supply opening or respectively discharge opening substantially opposite the filling opening and sample slide holding devices, arranged in the washing container such that they can keep the introduced sample slides apart, and a liquid discharge space remains between the introduced sample slides and the liquid supply opening or respectively discharge opening. The invention furthermore relates to a sample support washing station for washing sample slides, with at least one take-up for receiving an inventive sample support washing container, whereby the take-up has a station supply/discharge, which corresponds to its liquid supply opening or respectively discharge opening when the sample support washing container is removed, an overflow vessel arranged around the take-up, a pump connected to the station supply/discharge and at least one liquid supply valve connected to the pump. Finally, the invention relates to a system for washing sample slides with an inventive sample support washing station and at least an inventive sample support washing container and a method for washing and drying sample slides using an inventive sample support washing station and at least an inventive sample support washing container.

摘要翻译： 本发明涉及一种用于同时洗涤至少两个样品载玻片的样品支撑清洗容器，其中可以引入样品载玻片的填充开口，基本上与填充开口相对的液体供给开口或样品载玻片保持 装置，布置在洗涤容器中，使得它们可以保持引入的样品滑动分开，并且在引入的样品载片和液体供应开口或分别的排出口之间留有液体排出空间。 本发明还涉及一种用于清洗样品载玻片的样品支撑清洗站，至少一个用于容纳本发明的样品支架清洗容器的吸收器，其中吸收器具有对应于其液体供应开口 或者当取出样品支架清洗容器时分别排出开口，设置在卷取器周围的溢流容器，连接到站供给/排出的泵和连接到泵的至少一个液体供应阀。 最后，本发明涉及用本发明的样品支持清洗站和至少本发明的样品支持清洗容器洗涤样品载玻片的系统，以及使用本发明的样品支持清洗站和至少本发明的样品洗涤和干燥样品载玻片的方法 支持清洗容器。