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    CO-CULTURE DEVICE ASSEMBLY
    1.
    发明申请
    CO-CULTURE DEVICE ASSEMBLY 有权
    文化设备组合

    公开(公告)号：US20130143254A1

    公开(公告)日：2013-06-06

    申请号：US13309209

    申请日：2011-12-01

    申请人： Donald Thomas ,  Wei Li ,  Ning Ke ,  Nan Li ,  Adrian Papas ,  Manfred Watzele ,  Alexander Seiler ,  Tianxing Wang ,  Yama Abassi ,  Xiaobo Wang

    发明人： Donald Thomas ,  Wei Li ,  Ning Ke ,  Nan Li ,  Adrian Papas ,  Manfred Watzele ,  Alexander Seiler ,  Tianxing Wang ,  Yama Abassi ,  Xiaobo Wang

    IPC分类号： G01N27/02 ,  G01N21/00 ,  C12M1/34 ,  C12Q1/02 ,  C12M1/12 ,  C12M1/00

    CPC分类号： C12M25/04 ,  C12Q1/02 ,  G01N21/253 ,  G01N21/6452 ,  G01N33/48735 ,  G01N33/54373

    摘要： An insert device and co-culture device assembly incorporating the insert device, which includes an insert chamber with two fluid impermeable side walls that extend from a microporous bottom to an open top to form an inner cavity. The first side wall is shaped to form a convex arc that follows between about half of a circumference of the well and less than an entire circumference of the well. The second side wall joins ends of the first side wall and protrudes inward towards a center of the convex arc. A flange extends outward from or beneath the top and is notched to form a gap adjacent to the second side wall, which forms an access port allowing access to the lower chamber with a micropipette tip when the insert device is inserted into a well of a single or multi-well plate.

    摘要翻译： 包括插入装置的插入装置和共培养装置组件，其包括具有从微孔底部延伸到开口顶部以形成内腔的两个流体不可渗透侧壁的插入室。 第一侧壁成形为形成沿着井的圆周的大约一半且小于井的整个圆周的凸弧。 第二侧壁连接第一侧壁的端部并朝向凸弧的中心向内突出。 凸缘从顶部向下或从顶部向外延伸并且被切口以形成与第二侧壁相邻的间隙，当形成插入装置插入到单个孔中时，形成允许通过微量移液管尖端进入下腔室的进入口 或多孔板。

    Inhibition of peroxidase enzymatic activity
    4.
    发明授权
    Inhibition of peroxidase enzymatic activity 有权
    抑制过氧化物酶酶活性

    公开(公告)号：US08735083B2

    公开(公告)日：2014-05-27

    申请号：US11758854

    申请日：2007-06-06

    申请人： Manfred Watzele ,  Bernd Buchberger ,  Claudia Kirr

    发明人： Manfred Watzele ,  Bernd Buchberger ,  Claudia Kirr

    IPC分类号： G01N33/53 ,  C12N9/99

    CPC分类号： G01N33/573 ,  C12Q1/28 ,  G01N2333/908

    摘要： The present invention deals with reagents and compositions capable of effectively inhibiting peroxidase activity. According to the invention, peroxidase enzymatic activity is blocked with an acidic aqueous solution of a protein denaturing agent. preferred protein denaturing agents are detergents and chaotropic substances.

    摘要翻译： 本发明涉及能够有效抑制过氧化物酶活性的试剂和组合物。 根据本发明，过氧化物酶酶活性用蛋白质变性剂的酸性水溶液封闭。 优选的蛋白质变性剂是洗涤剂和离液剂。

    USE OF TDE FOR ISOLATION OF NUCLEIC ACIDS
    6.
    发明申请
    USE OF TDE FOR ISOLATION OF NUCLEIC ACIDS 审中-公开
    使用TDE分离核酸

    公开(公告)号：US20110266172A1

    公开(公告)日：2011-11-03

    申请号：US13182691

    申请日：2011-07-14

    申请人： Horst Donner ,  Frank Bergmann ,  Nina Lassonczyk ,  Manfred Watzele ,  Marcus Schmid

    发明人： Horst Donner ,  Frank Bergmann ,  Nina Lassonczyk ,  Manfred Watzele ,  Marcus Schmid

    IPC分类号： B65D71/00 ,  C12Q1/68 ,  C07H1/06

    CPC分类号： C12N15/1006

    摘要： The invention provides the use of tetraethylene glycol dimethyl ether for adsorbing nucleic acids to solid phases such as those with silica surfaces. To this end, the invention also provides compositions comprising TDE. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. Particularly, the invention encompasses methods for the purification of nucleic acids with low molecular weight. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.

    摘要翻译： 本发明提供四乙二醇二甲醚用于将核酸吸附到固相上，例如具有二氧化硅表面的那些。 为此，本发明还提供了包含TDE的组合物。 方法被公开并要求从样品中纯化核酸，以及用于实施这些方法的试剂盒。 特别地，本发明包括用于纯化低分子量核酸的方法。 通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。

    CHROMOGENIC TESTS IN THE PRESENCE OF CELLS
    7.
    发明申请
    CHROMOGENIC TESTS IN THE PRESENCE OF CELLS 审中-公开
    细胞存在的染色体检测

    公开(公告)号：US20070154975A1

    公开(公告)日：2007-07-05

    申请号：US11608943

    申请日：2006-12-11

    申请人： Manfred Watzele ,  Thomas Nikolaus ,  Hans-Juergen Rode

    发明人： Manfred Watzele ,  Thomas Nikolaus ,  Hans-Juergen Rode

    IPC分类号： C12Q1/37

    CPC分类号： G01N21/78 ,  C12Q1/00 ,  C12Q1/25 ,  G01N21/82 ,  G01N33/52

    摘要： The present invention concerns a method for the colorimetric measurement of enzyme activities comprising the following steps: preparing a cell suspension or a cell lysate containing cell components adding a chromogenic substrate at the start of the enzyme reaction adding a first additional substance to stop the enzyme reaction colorimetric measurement wherein a second additional substance which reduces the light scattering and/or absorption caused by the cells or the cell components is added during or after termination of the enzyme reaction.

    摘要翻译： 本发明涉及一种酶活性比色测定方法，包括以下步骤：在酶反应开始时，制备含有细胞组分的细胞悬液或含细胞裂解液的细胞成分，加入第一种附加物质以停止酶反应 比色测量，其中在酶反应终止期间或之后添加减少由细胞或细胞成分引起的光散射和/或吸收的第二附加物质。

    Enhanced expression of fusion polypeptides with a biotinylation tag
    8.
    发明申请
    Enhanced expression of fusion polypeptides with a biotinylation tag 审中-公开
    用生物素标记增强融合多肽的表达

    公开(公告)号：US20060046285A1

    公开(公告)日：2006-03-02

    申请号：US11211339

    申请日：2005-08-25

    申请人： Manfred Watzele ,  Dieter Voges ,  Frank Wedekind

    发明人： Manfred Watzele ,  Dieter Voges ,  Frank Wedekind

    IPC分类号： C12P21/04 ,  C12N9/10 ,  C07H21/04

    CPC分类号： C07K1/1077 ,  C07K2319/00

    摘要： The invention provides the means to enhance in E. coli-based expression systems the formation of fusion polypeptides containing as an N-terminal tag a biotinylation polypeptide. By way of specifically exchanging in the nucleic acid sequence encoding the biotinylation polypeptide nucleotides at 11 discrete positions enhances the formation of the total fusion polypeptide by at least 40%.

    摘要翻译： 本发明提供了在基于大肠杆菌的表达系统中增强含有作为N-末端标签的融合多肽生物素化多肽的方法。 通过在11个离散位置上特异性交换编码生物素化多肽核苷酸的核酸序列增强总融合多肽的形成至少40％。

    Reducing sample turbidity in measurement of enzymatic activity in cell lysates
    10.
    发明授权
    Reducing sample turbidity in measurement of enzymatic activity in cell lysates 失效
    在细胞裂解物中酶活性的测量中减少样品浊度

    公开(公告)号：US07919268B2

    公开(公告)日：2011-04-05

    申请号：US12409038

    申请日：2009-03-23

    申请人： Manfred Watzele ,  Thomas Nikolaus ,  Hans-Juergen Rode

    发明人： Manfred Watzele ,  Thomas Nikolaus ,  Hans-Juergen Rode

    IPC分类号： C12Q1/32

    CPC分类号： G01N21/78 ,  C12Q1/00 ,  C12Q1/25 ,  G01N21/82 ,  G01N33/52

    摘要： Disclosed are methods and kits for measuring enzyme activity in which the turbidity of a sample caused by cell components is minimized by particular additives. The methods include the steps of providing a cell suspension or a cell lysate containing cell components, adding a chromogenic substrate at the start of the enzyme reaction, adding a first additional substance to stop the enzyme reaction, and making a colorimetric measurement, wherein a second additional substance which reduces the light scattering and/or absorption caused by the cells or the cell components is added during or after termination of the enzyme reaction. In particular, a method is disclosed wherein the enzyme is lactate dehydrogenase (LDH) and the first additional substance to stop the reaction is HCl.

    摘要翻译： 公开了用于测量酶活性的方法和试剂盒，其中由细胞组分引起的样品的浊度被特定的添加剂最小化。 所述方法包括以下步骤：提供含有细胞成分的细胞悬液或细胞裂解物，在酶反应开始时加入显色底物，加入第一附加物质以停止酶反应，进行比色测定，其中第二 在酶反应终止期间或之后添加减少由细胞或细胞成分引起的光散射和/或吸收的附加物质。 特别地，公开了一种方法，其中酶是乳酸脱氢酶（LDH），停止反应的第一种另外的物质是HCl。