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    Method of separating biomolecules using nanopore
    4.
    发明申请
    Method of separating biomolecules using nanopore 审中-公开
    使用纳米孔分离生物分子的方法

    公开(公告)号：US20060183112A1

    公开(公告)日：2006-08-17

    申请号：US11335246

    申请日：2006-01-19

    申请人： Jun-hong Min ,  Su-hyeon Kim ,  In-ho Lee ,  Kui-hyun Kim ,  Seung-yeon Yang

    发明人： Jun-hong Min ,  Su-hyeon Kim ,  In-ho Lee ,  Kui-hyun Kim ,  Seung-yeon Yang

    IPC分类号： C12Q1/70 ,  C12Q1/68

    CPC分类号： G01N33/5438 ,  G01N33/48721

    摘要： Provided is a method of separating particles, the method comprising: forming a first chamber and a second chamber separated by an interface with a pore, wherein the first and second chambers have electrodes with different polarities; placing particles to which a target biomolecule is bound from particles to which the target biomolecule is not bound in the first chamber; applying a voltage which has the same polarity as that of the target biomolecule to the electrode of the first chamber, and a voltage which has an opposite charge to that of the target biomolecule to the electrode of the second chamber; and translocating only the particles to which the target biomolecule is bound from the first chamber to the second chamber through the pore. Conventionally, the size of a pore is used to separate biomolecules. However, effective separation is difficult to achieve because the manufacture of a pore with a diameter of less than 10 nm, small enough to separate biomolecule, is not easy. Therefore, signal separation and data analysis must be required. However, in the present method, physical movement induced by the charge of biomolecules is used to effectively separate the biomolecules, thus obtaining a high signal to noise ratio. As a result, additional data analysis is not required.

    摘要翻译： 提供了一种分离颗粒的方法，该方法包括：形成由与孔的界面分隔开的第一室和第二室，其中第一和第二室具有不同极性的电极; 在第一室中放置靶生物分子所结合的颗粒与目标生物分子不结合的颗粒; 将具有与目标生物分子相同极性的电压施加到第一室的电极，以及将具有与靶生物分子相反的电荷的电压施加到第二室的电极; 并且仅将来自第一室的目标生物分子结合的颗粒通过孔转移到第二室。 通常，使用孔的大小来分离生物分子。 然而，由于直径小于10nm的孔的制造足够小以分离生物分子，所以难以实现有效的分离，这是不容易的。 因此，必须要求信号分离和数据分析。 然而，在本方法中，由生物分子的电荷引起的身体运动用于有效分离生物分子，从而获得高的信噪比。 因此，不需要额外的数据分析。

    Method and apparatus for concentrating and amplifying nucleic acid in single micro chamber
    5.
    发明授权
    Method and apparatus for concentrating and amplifying nucleic acid in single micro chamber 有权
    在单微室中浓缩和扩增核酸的方法和装置

    公开(公告)号：US07807360B2

    公开(公告)日：2010-10-05

    申请号：US11620961

    申请日：2007-01-08

    申请人： Young-rok Kim ,  Jun-hong Min ,  In-ho Lee ,  Young-sun Lee ,  Chang-eun Yoo ,  Ki-woong Han

    发明人： Young-rok Kim ,  Jun-hong Min ,  In-ho Lee ,  Young-sun Lee ,  Chang-eun Yoo ,  Ki-woong Han

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6806 ,  B01L3/5027 ,  B01L3/5088 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2537/149 ,  C12Q2527/125

    摘要： A method of sequentially performing concentration and amplification of nucleic acid in a single micro chamber includes: introducing a nucleic acid-containing sample and a solution including a kosmotropic salt to a micro chamber having a hydrophilic interior surface to concentrate the nucleic acid by binding the nucleic acid on the interior surface of the micro chamber; and performing a polymerase chain reaction (PCR) by adding a PCR mixture to the chamber. Since the nucleic acid is reversibly bound to the interior surface of the micro chamber, PCR yield is higher compared with a surface of aluminum oxide in which irreversible binding occurs. In addition, all processes are sequentially performed in a single micro chamber so that the number of samples, consumables, time, and labor for treatment and analysis can be reduced, detection sensitivity can be improved, and risk of sample cross contamination significantly reduced without sample loss by eliminating transporting of the sample. A complete automated system for concentration and amplification of nucleic acid is thus readily provided.

    摘要翻译： 在单个微室中依次进行核酸的浓缩和扩增的方法包括：将含核酸的样品和包含可染色盐的溶液引入具有亲水性内表面的微室中，以通过结合核酸来浓缩核酸 酸在微室的内表面上; 并通过向该室加入PCR混合物进行聚合酶链式反应（PCR）。 由于核酸可逆地结合到微室的内表面，与其中发生不可逆结合的氧化铝的表面相比，PCR产率更高。 此外，所有过程都在单个微室中顺序进行，以便可以减少样品数量，消耗品，时间和处理和分析的劳动力，可以提高检测灵敏度，并且样品交叉污染的风险在没有样品时显着降低 通过消除样品的运输而损失。 因此容易提供用于浓缩和扩增核酸的完整的自动化系统。