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    DNA SEQUENCING METHODS AND DETECTORS AND SYSTEMS FOR CARRYING OUT THE SAME
    4.
    发明申请
    DNA SEQUENCING METHODS AND DETECTORS AND SYSTEMS FOR CARRYING OUT THE SAME 有权
    DNA序列方法和检测器及其执行系统

    公开(公告)号：US20110236984A1

    公开(公告)日：2011-09-29

    申请号：US12984269

    申请日：2011-01-04

    申请人： Hongye Sun ,  Eric S. Nordman ,  Mark F. Oldham ,  John R. O'Neill ,  Charles Connell ,  Umberto Ulmanella ,  Aldrich N.K. Lau ,  Theofilos Kotseroglou ,  Kenneth J. Livak

    发明人： Hongye Sun ,  Eric S. Nordman ,  Mark F. Oldham ,  John R. O'Neill ,  Charles Connell ,  Umberto Ulmanella ,  Aldrich N.K. Lau ,  Theofilos Kotseroglou ,  Kenneth J. Livak

    IPC分类号： G01N27/447 ,  G01Q60/24 ,  C08G69/42 ,  B82Y5/00

    CPC分类号： C12Q1/6869 ,  B01L3/502761 ,  G01N27/3278 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  Y10S977/924 ,  Y10T436/143333 ,  C12Q2565/631

    摘要： In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures. Surface-modified nanopores are provided as are methods of making them. In some embodiments, surfaced-modified nanopores for slowing the translocation of single stranded DNA (ssDNA) through the nanopore are provided, as are nanopores configured to detect each of a plurality of different bases on an ssDNA strand.

    摘要翻译： 在一些实施例中，提供了分析物检测系统，其包括纳米通道，电极布置以及设置在纳米通道中的多个纳米器件器件。 可以使用多个核酸碱基检测组分，其包括多个纳米孔，多个纳米通道，多个杂交探针及其组合等。 根据本教导的其他实施方案，将不同的编码分子与靶DNA分子杂交并用于检测沿着靶分子的各种序列的存在。 还提供了包含编码分子混合物的试剂盒。 在一些实施方案中，包括纳米通道，纳米孔等的装置用于操纵DNA分子的移动，例如用于DNA测序检测的准备。 还提供了制备其的纳米孔结构及其制备方法，使用纳米孔结构的核酸测序方法也是如此。 提供表面改性的纳米孔是制备它们的方法。 在一些实施方案中，提供了用于减缓通过纳米孔的单链DNA（ssDNA）易位的表面改性的纳米孔，以及配置成检测ssDNA链上的多个不同碱基中的每一个的纳米孔。

    DEVICES AND METHODS FOR OPTOELECTRONIC MANIPULATION OF SMALL PARTICLES
    6.
    发明申请
    DEVICES AND METHODS FOR OPTOELECTRONIC MANIPULATION OF SMALL PARTICLES 有权
    用于光电子操作小颗粒的装置和方法

    公开(公告)号：US20100206731A1

    公开(公告)日：2010-08-19

    申请号：US12702137

    申请日：2010-02-08

    申请人： Aldrich N.K. Lau ,  Joon Mo Yang ,  Huan L. Phan ,  Steven W. Sherwood ,  Hans A. Fuernkranz

    发明人： Aldrich N.K. Lau ,  Joon Mo Yang ,  Huan L. Phan ,  Steven W. Sherwood ,  Hans A. Fuernkranz

    IPC分类号： B03C11/00

    CPC分类号： B03C5/026 ,  B03C5/005 ,  B03C5/028 ,  C07K1/26 ,  G01N27/305

    摘要： A method for sorting cells in a biological sample comprising a first type of cells and a second type of cells may comprise introducing the biological sample into a chamber comprising a first surface and a second surface, wherein the first surface is associated with a transparent electrode and the second surface is associated with a photoconductive portion of an electrode. The method may further comprise moving incident light and the photoconductive portion relative to one another so as to illuminate regions of the photoconductive portion and modulate an electric field in the chamber in proximity to the illuminated regions. The method may further comprise separating the first type of cells from the second type of cells in the chamber via dielectrophoretic movement of the first type of cells and the second type of cells caused by the modulated electric field, wherein a dielectrophoretic characteristic of at least one of the first type of cells and the second type of cells has been modified.

    摘要翻译： 用于分选生物样品中包含第一类型细胞和第二类型细胞的细胞的方法可以包括将生物样品引入包含第一表面和第二表面的室中，其中第一表面与透明电极相关联， 第二表面与电极的光电导部分相关联。 该方法还可以包括相对于彼此移动入射光和光电导部分，以照射光电导部分的区域并且调制在邻近照明区域的室中的电场。 该方法还可以包括通过由调制电场引起的第一类型的细胞和第二类型的细胞的介电电泳运动，将第一类型的细胞与腔室中的第二类型的细胞分离，其中至少一种的介电电泳特性 的第一类型的细胞和第二类型的细胞已被修改。

    Surface Modification in a Manipulation Chamber
    8.
    发明申请
    Surface Modification in a Manipulation Chamber 审中-公开
    操纵室中的表面改性

    公开(公告)号：US20090233327A1

    公开(公告)日：2009-09-17

    申请号：US12398140

    申请日：2009-03-04

    申请人： Aldrich N.K. Lau ,  Huan L. Phan

    发明人： Aldrich N.K. Lau ,  Huan L. Phan

    IPC分类号： C12Q1/02 ,  B05D5/12 ,  B05D3/00

    CPC分类号： G01N27/305 ,  B03C11/00 ,  C07K1/26

    摘要： A device for manipulating biological material, the device including at least one electrode and a photoconductive material configured to receive the biological material; and a light source configured to illuminate the photoconductive material so as to modulate an electric field, wherein the electric field is configured to manipulate the biological material; wherein a surface of the at least one electrode and/or the photoconductive material is modified with at least one of a carboxylic moiety, an amino moiety, a poly(ethylene glycol) moiety, a polymer of (poly(ethylene oxide) methyl ether) acrylate, a poly(2-hydroxyethyl (meth)acrylate), a poly(N-vinylpyrrolidone), a poly(N-vinylformamide), a poly(N-vinylformamide) derivative, a poly((meth)acrylamide), and a poly((meth)acrylamide) derivative. Methods for manipulating biological material are also disclosed.

    摘要翻译： 一种用于操纵生物材料的装置，该装置包括至少一个电极和被配置为容纳生物材料的光导材料; 以及光源，被配置为照亮所述光电导材料以调制电场，其中所述电场被配置为操纵所述生物材料; 其中所述至少一个电极和/或光电导材料的表面用羧基部分，氨基部分，聚（乙二醇）部分，（聚（环氧乙烷）甲基醚）的聚合物， 丙烯酸酯，聚（甲基）丙烯酸2-羟乙酯），聚（N-乙烯基吡咯烷酮），聚（N-乙烯基甲酰胺），聚（N-乙烯基甲酰胺）衍生物，聚（（甲基）丙烯酰胺） 聚（（甲基）丙烯酰胺）衍生物。 还公开了用于操作生物材料的方法。