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19 条记录 (耗时 0.024 秒)

    Repuncturable self-sealing sample container with internal collapsible bag
    1.
    发明授权
    Repuncturable self-sealing sample container with internal collapsible bag 失效
    具有内部可折叠袋的可重复自密封样品容器

    公开(公告)号：US06805842B1

    公开(公告)日：2004-10-19

    申请号：US09976628

    申请日：2001-10-12

    申请人： Kevin S. Bodner ,  Tyler A. Palmer ,  Pejman Ghanouni

    发明人： Kevin S. Bodner ,  Tyler A. Palmer ,  Pejman Ghanouni

    IPC分类号： B01L300

    CPC分类号： B01L3/505 ,  B01L3/0217 ,  B01L3/50825 ,  B01L3/5085 ,  B01L2200/142 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/0672 ,  B01L2300/0854 ,  B01L2400/0478

    摘要： A sample container for minimizing evaporation of a contained volume of sample includes a container housing, a repuncturable self-sealing membrane, and a collapsible sample bag. The container housing includes an open end and a hollow interior region. The repuncturable self-sealing membrane configured to self-seal after repeated punctures is engaged in the open end of the container housing and includes an exterior surface exposed to the external environment and an interior surface oriented toward the hollow interior region of the container housing. The collapsible sample bag includes a proximate end that is permanently attached to the interior surface of the repuncturable self-sealing membrane.

    摘要翻译： 用于最小化容纳体积的样品的蒸发的样品容器包括容器壳体，可重复的自密封膜和可折叠样品袋。 容器壳体包括开口端和中空的内部区域。 构造为在重复穿刺之后自密封的可重复使用的自密封膜被接合在容器壳体的开口端中，并且包括暴露于外部环境的外表面和朝向容器壳体的中空内部区域定向的内表面。 可折叠的样品袋包括永久地附着到可再次自密封膜的内表面的近端。

    Purification apparatus and method
    4.
    发明授权
    Purification apparatus and method 失效
    净化装置及方法

    公开(公告)号：US06419827B1

    公开(公告)日：2002-07-16

    申请号：US09552301

    申请日：2000-04-18

    申请人： Donald R. Sandell ,  Kevin S. Bodner ,  Mark Borodkin ,  Mark Oldham ,  Jon Hoshizaki

    发明人： Donald R. Sandell ,  Kevin S. Bodner ,  Mark Borodkin ,  Mark Oldham ,  Jon Hoshizaki

    IPC分类号： B01D6300

    CPC分类号： B01L3/50255 ,  B01D61/18 ,  B01L3/5025 ,  B01L2400/049 ,  G01N35/0099 ,  G01N35/028 ,  G01N35/1074 ,  G01N2035/00485 ,  G01N2035/102

    摘要： The invention includes a filtration apparatus for processing a plurality of fluid samples into sample wells is provided. In one embodiment, the filtration apparatus includes a purification tray and a sample well tray with a plurality of sample wells. The purification tray includes a filter plate having a plurality of columns with discharge openings at the bottom thereof, at least one filter positioned in the columns of the filter plate for filtering the fluid samples as they pass therethrough, a heat plate positioned adjacent the columns of the filter plate, and a vent plate positioned below the heat plate. The heat plate is configured for transferring heat to the columns of the filter plate. The vent plate includes vents for permitting aerosols from the sample wells of the sample well tray to escape. A sample well tray with a plurality of the sample wells is positioned so that the sample wells align with the discharge openings of the filter plate columns to receive the liquid sample therein.

    摘要翻译： 本发明提供了一种用于将多个流体样品加工成样品孔的过滤装置。 在一个实施例中，过滤装置包括净化盘和具有多个样品孔的样品井托盘。 净化盘包括一个过滤板，该过滤板具有多个在其底部具有排放口的柱，至少一个过滤器，定位在过滤板的列中，用于在流体样品通过过滤器时对其进行过滤， 过滤板和位于加热板下方的通风板。 加热板被配置为将热量传递到过滤板的列。 通风板包括用于允许来自样品井托盘的样品井的气溶胶逸出的通风口。 定位具有多个样品孔的样品孔盘，使得样品孔与过滤板柱的排出口对准以在其中接收液体样品。

    System for real time detection of nucleic acid amplification products
    5.
    发明授权
    System for real time detection of nucleic acid amplification products 失效
    核酸扩增产物实时检测系统

    公开(公告)号：US5928907A

    公开(公告)日：1999-07-27

    申请号：US752973

    申请日：1996-12-02

    申请人： Timothy M. Woudenberg ,  Kevin S. Bodner ,  Charles R. Connell ,  Alan M. Ganz ,  Lincoln J. McBride ,  Paul G. Saviano ,  John Shigeura ,  David H. Tracy ,  Eugene F. Young ,  Linda G. Lee

    发明人： Timothy M. Woudenberg ,  Kevin S. Bodner ,  Charles R. Connell ,  Alan M. Ganz ,  Lincoln J. McBride ,  Paul G. Saviano ,  John Shigeura ,  David H. Tracy ,  Eugene F. Young ,  Linda G. Lee

    IPC分类号： C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  G01N21/03 ,  G01N21/64 ,  G01N21/75 ,  G01N21/78 ,  G01N33/50 ,  G01N33/58

    CPC分类号： G01N21/6428 ,  C12Q1/6818 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N21/0303 ,  G01N2021/6484

    摘要： A system is provided for carrying out real time fluorescence-based measurements of nucleic acid amplification products. In a preferred embodiment of the invention, an excitation beam is focused into a reaction mixture through a surface, the reaction mixture containing (i) a first fluorescent indicator capable of generating a first fluorescent signal whose intensity is proportional to the amount of an amplification product in the volume of the reaction mixture illuminated by the excitation beam and (ii) a second fluorescent indicator homogeneously distributed throughout the reaction mixture capable of generating a second fluorescent signal proportional to the volume of reaction mixture illuminated by the excitation beam. Preferably, the excitation beam is focused into the reaction mixture by a lens through a portion of a wall of a closed reaction chamber containing the reaction mixture. The same lens is used to collect the first and second fluorescent signals generated by the first and second fluorescent indicators, respectively, in response to the excitation beam. The ratio of the fluorescent intensities of the first and second fluorescent signals provides a stable quantitative indicator of the amount of amplification product synthesized in the course of the amplification reaction.

    摘要翻译： 提供了一种用于进行核酸扩增产物的实时基于荧光的测量的系统。 在本发明的优选实施方案中，激发光束通过表面聚焦到反应混合物中，反应混合物含有（i）第一荧光指示剂，其能够产生第一荧光信号，其强度与扩增产物的量成比例 在由激发束照射的反应混合物的体积中和（ii）均匀分布在整个反应混合物中的第二荧光指示器，其能够产生与由激发束照射的反应混合物的体积成比例的第二荧光信号。 优选地，激发光束通过透镜通过包含反应混合物的封闭反应室的壁的一部分聚焦到反应混合物中。 相同的透镜被用于响应于激发光束分别收集由第一和第二荧光指示器产生的第一和第二荧光信号。 第一和第二荧光信号的荧光强度比提供了在扩增反应过程中合成的扩增产物的量的稳定的定量指标。

    Multi-well microfiltration apparatus
    10.
    发明授权
    Multi-well microfiltration apparatus 有权
    多孔微滤装置

    公开(公告)号：US6159368A

    公开(公告)日：2000-12-12

    申请号：US182946

    申请日：1998-10-29

    申请人： Stephen E. Moring ,  Kevin S. Bodner ,  Jason H. Halsey ,  Jon Hoshizaki ,  Joseph Jackson ,  Alfred P. Madden ,  Mark F. Oldham ,  Mark T. Reed

    发明人： Stephen E. Moring ,  Kevin S. Bodner ,  Jason H. Halsey ,  Jon Hoshizaki ,  Joseph Jackson ,  Alfred P. Madden ,  Mark F. Oldham ,  Mark T. Reed

    IPC分类号： G01N1/00 ,  B01D29/00 ,  B01D35/02 ,  B01D53/22 ,  B01D61/18 ,  B01L3/00 ,  C12N15/09 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10 ,  B01D63/00

    CPC分类号： B01L3/5025 ,  B01D61/18 ,  B01L3/50255 ,  G01N35/0099 ,  B01L2400/049 ,  G01N2035/00485 ,  G01N2035/102 ,  G01N35/028 ,  G01N35/1074 ,  Y10T436/25

    摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with is the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.

    摘要翻译： 本发明提供了多孔板和柱阵列，其中可以分析和/或处理样品（例如，含有目标核酸的细胞裂解物，例如RNA）。 在一个实施方案中，微滤装置是多层结构，包括（i）具有可以放置样品的小柱阵列的柱板，（ii）设置在每个小柱中的离散过滤元件，（iii）滴液导向器 板具有相应的滴液导向器阵列，滤液可以通过其排出;以及（iv）接收孔板，其具有滤液能够流过的相应阵列的接收孔。 本发明提供了相对简单的多孔微过滤装置，并且克服了许多与以下问题有关的问题：（i）由于穿过公共过滤片芯线而引起的交叉污染或（ii）单个过滤元件 在大量死体积内捕获样品成分。 此外，本发明提供了多孔微过滤装置，其充分地支撑设置在井中的分立的过滤元件，而不产生实质的优先流动。 另外，本发明提供了多孔微量过滤装置，其避免了由于气溶胶形成，悬挂液滴和/或飞溅而导致的交叉污染。 本发明的其它公开的特征提供了分别收集在孔阵列中的滤液样品的自动覆盖或热封。