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45 条记录 (耗时 0.027 秒)

    Method of detecting a substance to be analyzed
    1.
    发明授权
    Method of detecting a substance to be analyzed 失效
    检测待分析物质的方法

    公开(公告)号：US06503714B1

    公开(公告)日：2003-01-07

    申请号：US09713031

    申请日：2000-11-16

    申请人： Kurt Weindel ,  Christoph Seidel ,  Gerhard Lassonczyk

    发明人： Kurt Weindel ,  Christoph Seidel ,  Gerhard Lassonczyk

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6813 ,  C12Q1/6816

    摘要： The invention concerns a method of detecting a substance to be analyzed in a sample by contacting the sample with a probe which contains nucleobases and two or more non-nucleosidic marker-promoting groups in conditions in which the substance to be analyzed binds indirectly or directly to the probe. The method enables the binding product to be detected. The probe is preferably branched.

    摘要翻译： 本发明涉及通过使样品与包含核碱基和两个或多个非核苷酸标志物促进基团的探针在待分析的物质间接或直接结合的条件下接触样品来检测待分析物质的方法， 探针。 该方法能够检测结合产物。 探针优选是支链的。

    Method of detecting a substance to be analyzed
    2.
    发明授权
    Method of detecting a substance to be analyzed 失效
    检测待分析物质的方法

    公开(公告)号：US06214549B1

    公开(公告)日：2001-04-10

    申请号：US09051079

    申请日：1998-09-10

    申请人： Kurt Weindel ,  Christoph Seidel ,  Gerhard Lassonczyk

    发明人： Kurt Weindel ,  Christoph Seidel ,  Gerhard Lassonczyk

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6813 ,  C12Q1/6816

    摘要： The invention concerns a method of detecting a substance to be analyzed in a sample by contacting the sample with a probe which contains nucleobases and two or more non-nucleosidic marker-promoting groups in conditions in which the substance to be analyzed binds indirectly or directly to the probe. The method enables the binding product to be detected. The probe is preferably branched.

    摘要翻译： 本发明涉及通过使样品与包含核碱基和两个或多个非核苷酸标志物促进基团的探针在待分析的物质间接或直接结合的条件下接触样品来检测待分析物质的方法， 探针。 该方法能够检测结合产物。 探针优选是支链的。

    Nucleic acids encoding fusion peptides
    5.
    发明授权
    Nucleic acids encoding fusion peptides 有权
    编码融合肽的核酸

    公开(公告)号：US07348423B2

    公开(公告)日：2008-03-25

    申请号：US10969624

    申请日：2004-10-20

    申请人： Eva Hoess ,  Thomas Meier ,  Gabriele Pestlin ,  Friedrich Popp ,  Klaus Reichert ,  Rainer Schmuck ,  Bernd Schneidinger ,  Christoph Seidel ,  Wilhelm Tischer

    发明人： Eva Hoess ,  Thomas Meier ,  Gabriele Pestlin ,  Friedrich Popp ,  Klaus Reichert ,  Rainer Schmuck ,  Bernd Schneidinger ,  Christoph Seidel ,  Wilhelm Tischer

    IPC分类号： A61K39/21

    CPC分类号： C07K14/005 ,  C07K2319/00 ,  C12N2740/15022 ,  C12N2740/16122 ,  C12P21/02

    摘要： A process is disclosed for the production of an antifusogenic peptide by producing a fusion peptide of a length of about 14 to 70 amino acids in a prokaryotic host cell, comprising the steps, under such conditions that inclusion bodies of said fusion peptide are formed, of: (a) expressing in said host cell a nucleic acid encoding said fusion peptide consisting of a first peptide which is an antifusogenic peptide of a length of about 10 to 50 amino acids and a second peptide of a length of about 4 to 30 amino acids, said first peptide being N-terminally linked to said second peptide; (b) cultivating said host cell to produce said inclusion bodies; and (c) recovering said antifusogenic peptide from said inclusion bodies, wherein said recovered antifusogenic peptide consists of said fusion peptide or a peptide comprising the antifusogenic peptide of about 10 to 50 amino acids and which is a fragment cleaved from said fusion peptide. Inclusion bodies of the peptides are disclosed. Also disclosed is a nucleic acid encoding the fusion peptide consisting of a first peptide which is an antifusogenic peptide selected from the group of SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, and said sequences further consisting of glycine at the C terminal end, N-terminally linked to the second peptide.

    摘要翻译： 公开了通过在原核宿主细胞中产生约14至70个氨基酸长度的融合肽来生产抗融合肽的方法，包括以下步骤：在形成所述融合肽的包涵体的条件下， （a）在所述宿主细胞中表达编码所述融合肽的核酸，所述融合肽由长度为约10至50个氨基酸的抗融合肽的第一肽和长度为约4-30个氨基酸的第二个肽组成 所述第一肽与所述第二肽N-末端连接; （b）培养所述宿主细胞以产生所述包涵体; 和（c）从所述包涵体中回收所述抗融合肽，其中所述回收的抗融合肽由所述融合肽或包含约10至50个氨基酸的抗融合肽的肽组成，并且是从所述融合肽切割的片段。 公开了肽的包涵体。 还公开了编码由第一肽组成的融合肽的核酸，第一肽是选自SEQ ID NO：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10的抗融合肽， 所述序列进一步由C末端的甘氨酸组成，N-末端与第二肽连接。