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    Normalisation of microarray data based on hybridisation with an internal reference
    2.
    发明申请
    Normalisation of microarray data based on hybridisation with an internal reference 审中-公开
    基于与内部参考杂交的微阵列数据的归一化

    公开(公告)号：US20050153290A1

    公开(公告)日：2005-07-14

    申请号：US10499476

    申请日：2002-12-17

    申请人： Marinus Gerardus Van Beuningen

    发明人： Marinus Gerardus Van Beuningen

    IPC分类号： C12M1/34 ,  C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G01N33/96 ,  G01N35/00 ,  G06F19/00

    CPC分类号： C12Q1/68 ,  C12M1/34 ,  G01N33/48 ,  G01N33/50 ,  G01N35/00594 ,  G01N35/00693 ,  G01N2035/00158 ,  G01N2496/00 ,  G16Z99/00

    摘要： The invention relates to methods and corresponding arrays especially suited to correct for signal errors due to variations in sample preparation. Methods and compositions for performing quantitative array-based assays are provided. In the subject methods, both a reporter and an analyte is employed, where the reporter is characterized by binding selectively to an internal reference present on the array, i.e. at least a subset of, if not all of, the spots present on the array employed in the method contain an internal reference which can be bound by reporter.

    摘要翻译： 本发明涉及特别适合于校正由于样品制备变化引起的信号误差的方法和相应阵列。 提供了用于进行定量阵列测定的方法和组合物。 在主题方法中，采用报告物和分析物，其中报道子的特征在于选择性地结合阵列上存在的内部参考，即至少一个子集，如果不是全部，则使用阵列上存在的点 在该方法中包含可由报告人约束的内部参考。

    Novel high density arrays and methods for analyte analysis
    4.
    发明申请
    Novel high density arrays and methods for analyte analysis 审中-公开
    用于分析物分析的新型高密度阵列和方法

    公开(公告)号：US20050202433A1

    公开(公告)日：2005-09-15

    申请号：US10515485

    申请日：2003-06-02

    申请人： Marinus Gerardus Van Beuningen

    发明人： Marinus Gerardus Van Beuningen

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： C12Q1/6823 ,  C12Q2565/501 ,  C12Q2523/319 ,  C12Q2525/131 ,  C12Q2527/107

    摘要： The present invention relates to methods for identifying analytes in a sample comprising the steps of: (a) incubating said analytes with a plurality of bipartite capture probes, said capture probes being immobilized in predefined regions on a solid substrate, and each capture probe consisting essentially of a first fragment which is at one end immobilized to said substrate and at the other end is complementary linked to a second fragment, wherein said second fragment comprises an extension fragment capable of identifying an analyte; (b) monitoring complex formation between sample analytes and extension fragments; (c) sequentially modifying complex formation conditions; allowing the release of captured analyte molecules from the substrate; and (d) detecting and identifying the released analytes. The present invention also relates to different uses of said methods as well as microarrays and kits for performing said methods.

    摘要翻译： 本发明涉及用于鉴定样品中分析物的方法，包括以下步骤：（a）将所述分析物与多个二分捕获探针一起孵育，所述捕获探针固定在固体基质上的预定区域中，并且每个捕获探针基本上组成 其一端固定在所述底物上，另一端与第二片段互补连接，其中所述第二片段包含能够鉴定分析物的延伸片段; （b）监测样品分析物和延伸片段之间的复合物形成; （c）依次修饰复合物形成条件; 允许从底物释放捕获的分析物分子; 和（d）检测和鉴定释放的分析物。 本发明还涉及所述方法的不同用途以及用于执行所述方法的微阵列和试剂盒。