利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

30 条记录 (耗时 0.017 秒)

    Micromanifold assembly
    1.
    发明授权
    Micromanifold assembly 有权
    Micromanifold组装

    公开(公告)号：US08163254B1

    公开(公告)日：2012-04-24

    申请号：US12476002

    申请日：2009-06-01

    申请人： Ronald F. Renzi ,  Scott M. Ferko

    发明人： Ronald F. Renzi ,  Scott M. Ferko

    IPC分类号： B01L3/00 ,  B01L3/02

    CPC分类号： B01L3/502715 ,  B01L3/565 ,  B01L9/527 ,  B01L2200/027 ,  G01N30/6026 ,  G01N30/6095

    摘要： A micromanifold for connecting external capillaries to the inlet and/or outlet ports of a microfluidic device can employ a ferrule/capillary assembly that includes: (a) a ferrule comprising an elongated member and having a bore traversing from a proximal end to a distal end of the member, wherein the bore has an inner surface and wherein the distal end of the ferrule has a tapered, threaded exterior surface, and (b) a capillary that is positioned within the bore wherein the capillary's outer surface is in direct contact with the bore's inner surface. No mating sleeve is required for the one-piece ferrule. Alternatively, the capillaries can be bonded to channels that traverse the manifold and therefore obviate the need for a ferrule.

    摘要翻译： 用于将外部毛细管连接到微流体装置的入口和/或出口的微型歧管可以采用套圈/毛细管组件，其包括：（a）套圈，其包括细长构件，并且具有从近端到远端的孔 ，其中所述孔具有内表面，并且其中所述套圈的远端具有锥形的带螺纹的外表面，以及（b）位于所述孔内的毛细管，其中所述毛细管的外表面与所述毛细管的外表面直接接触 孔的内表面。 单件套圈不需要配套套管。 或者，毛细管可以结合到穿过歧管的通道，因此避免了对套圈的需要。

    Microelectroporation device for genomic screening
    3.
    发明授权
    Microelectroporation device for genomic screening 有权
    用于基因组筛选的微电极装置

    公开(公告)号：US08828736B2

    公开(公告)日：2014-09-09

    申请号：US13173180

    申请日：2011-06-30

    申请人： Thomas D. Perroud ,  Ronald F. Renzi ,  Oscar Negrete ,  Mark R. Claudnic

    发明人： Thomas D. Perroud ,  Ronald F. Renzi ,  Oscar Negrete ,  Mark R. Claudnic

    IPC分类号： G01N25/08 ,  C12M1/42 ,  B01L3/00

    CPC分类号： B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0645 ,  B01L2300/0864 ,  C12M35/02 ,  Y10T436/2575

    摘要： We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.

    摘要翻译： 我们开发了一种微电极装置，其结合寡核苷酸微阵列，微流体通道和电穿孔用于细胞转染和高通量筛选应用（例如RNA干扰筛选）。 微阵列允许在微观斑点中沉积数千种不同的寡核苷酸。 微流体通道和微孔能够有效地将细胞装载到装置中并防止不同寡核苷酸斑点之间的交叉污染。 电穿孔允许通过最大限度地减少细胞死亡同时使转染效率最大化将核酸最佳地转染到细胞（特别是难转染细胞，如原代细胞）中。 本发明具有较高的生产能力和较低的成本，同时防止与常规筛选技术相比的交叉污染。 此外，该装置不需要庞大的机器人液体处理设备，并且由于它是封闭的系统，所以固有地更安全。

    ION CHAMBER BASED NEUTRON DETECTORS
    4.
    发明申请
    ION CHAMBER BASED NEUTRON DETECTORS 有权
    基于离子室的中子探测器

    公开(公告)号：US20130020492A1

    公开(公告)日：2013-01-24

    申请号：US13559370

    申请日：2012-07-26

    申请人： Mark S. Derzon ,  Paul C. Galambos ,  Ronald F. Renzi

    发明人： Mark S. Derzon ,  Paul C. Galambos ,  Ronald F. Renzi

    IPC分类号： G01T3/00

    CPC分类号： G01T3/008 ,  G01T3/00 ,  H01J47/02

    摘要： A neutron detector with monolithically integrated readout circuitry, including: a bonded semiconductor die; an ion chamber formed in the bonded semiconductor die; a first electrode and a second electrode formed in the ion chamber; a neutron absorbing material filling the ion chamber; and the readout circuitry which is electrically coupled to the first and second electrodes. The bonded semiconductor die includes an etched semiconductor substrate bonded to an active semiconductor substrate. The readout circuitry is formed in a portion of the active semiconductor substrate. The ion chamber has a substantially planar first surface on which the first electrode is formed and a substantially planar second surface, parallel to the first surface, on which the second electrode is formed. Desirably, the distance between the first electrode and the second electrode may be equal to or less than the 50% attenuation length for neutrons in the neutron absorbing material filling the ion chamber.

    摘要翻译： 一种具有单片集成读出电路的中子检测器，包括：键合半导体管芯; 形成在所述键合半导体管芯中的离子室; 形成在所述离子室中的第一电极和第二电极; 填充离子室的中子吸收材料; 以及电耦合到第一和第二电极的读出电路。 键合的半导体管芯包括结合到有源半导体衬底的蚀刻半导体衬底。 读出电路形成在有源半导体衬底的一部分中。 离子室具有基本上平面的第一表面，第一电极形成在其上，并且平行于第一表面的基本平坦的第二表面，在其上形成第二电极。 理想地，第一电极和第二电极之间的距离可以等于或小于填充离子室的中子吸收材料中的中子的50％的衰减长度。

    Modular optical detector system
    6.
    发明授权
    Modular optical detector system 有权
    模块化光学检测器系统

    公开(公告)号：US06998598B2

    公开(公告)日：2006-02-14

    申请号：US10633794

    申请日：2003-08-04

    申请人： Brent A. Horn ,  Ronald F. Renzi

    发明人： Brent A. Horn ,  Ronald F. Renzi

    IPC分类号： H01J3/14 ,  H01J40/14 ,  H01J5/16

    CPC分类号： G01N30/74 ,  B01L3/5027 ,  G01N21/645 ,  G01N2201/024

    摘要： A modular optical detector system. The detector system is designed to detect the presence of molecules or molecular species by inducing fluorescence with exciting radiation and detecting the emitted fluorescence. Because the system is capable of accurately detecting and measuring picomolar concentrations it is ideally suited for use with microchemical analysis systems generally and capillary chromatographic systems in particular. By employing a modular design, the detector system provides both the ability to replace various elements of the detector system without requiring extensive realignment or recalibration of the components as well as minimal user interaction with the system. In addition, the modular concept provides for the use and addition of a wide variety of components, including optical elements (lenses and filters), light sources, and detection means, to fit particular needs.

    摘要翻译： 模块化光学检测器系统。 探测器系统设计用于通过用激发辐射诱导荧光并检测发射的荧光来检测分子或分子物质的存在。 因为该系统能够精确地检测和测量皮摩尔浓度，所以它理想地适用于通常用于微量化学分析系统和特别是毛细管色谱系统。 通过采用模块化设计，检测器系统提供了替换检测器系统的各种元件的能力，而不需要对组件进行大量的重新对准或重新校准，以及最小化与系统的用户交互。 此外，模块化概念提供了各种组件的使用和添加，包括光学元件（透镜和滤光片），光源和检测装置，以适应特殊需要。

    High pressure capillary connector
    7.
    发明授权
    High pressure capillary connector 失效
    高压毛细管连接器

    公开(公告)号：US06926313B1

    公开(公告)日：2005-08-09

    申请号：US10405204

    申请日：2003-04-02

    申请人： Ronald F. Renzi

    发明人： Ronald F. Renzi

    IPC分类号： B01J19/00 ,  B01L3/00 ,  F04B43/04 ,  F04B53/16 ,  F16L19/06 ,  F16L49/06 ,  G01N30/36 ,  G01N30/60 ,  F16L17/02

    CPC分类号： B01J19/0093 ,  B01J2219/0081 ,  B01L3/502715 ,  B01L3/563 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/14 ,  F04B43/043 ,  F04B53/16 ,  G01N30/36 ,  G01N30/6026 ,  G01N30/6078 ,  G01N30/6095

    摘要： A high pressure connector capable of operating at pressures of 40,000 psi or higher is provided. This connector can be employed to position a first fluid-bearing conduit that has a proximal end and a distal end to a second fluid-bearing conduit thereby providing fluid communication between the first and second fluid-bearing conduits. The connector includes (a) an internal fitting assembly having a body cavity with (i) a lower segment that defines a lower segment aperture and (ii) an interiorly threaded upper segment, (b) a first member having a first member aperture that traverses its length wherein the first member aperture is configured to accommodate the first fluid-bearing conduit and wherein the first member is positioned in the lower segment of the internal fitting assembly, and (c) a second member having a second member aperture that traverses its length wherein the second member is positioned in the upper segment of the fitting assembly and wherein a lower surface of the second member is in contact with an upper surface of the first member to assert a compressive force onto the first member and wherein the first member aperture and the second member aperture are coaxial.

    摘要翻译： 提供能够在40,000psi或更高压力下操作的高压连接器。 该连接器可用于将具有近端和远端的第一流体承载管道定位到第二流体承载导管，从而提供第一和第二流体承载导管之间的流体连通。 连接器包括（a）具有体腔的内部配件组件，其具有限定下部分段孔的下部段和（ii）内部带螺纹的上部段，（b）第一构件，其具有穿过第一构件孔 其长度，其中所述第一构件孔被构造成容纳所述第一流体承载导管，并且其中所述第一构件定位在所述内部装配组件的下部段中，以及（c）第二构件，其具有穿过其长度的第二构件孔 其中所述第二构件定位在所述装配组件的上部段中，并且其中所述第二构件的下表面与所述第一构件的上表面接触以将压缩力施加到所述第一构件上，并且其中所述第一构件孔和 第二构件孔是同轴的。

    Capillary interconnect device
    8.
    发明授权
    Capillary interconnect device 有权
    毛细管互连装置

    公开(公告)号：US08585986B1

    公开(公告)日：2013-11-19

    申请号：US11983440

    申请日：2007-11-09

    申请人： Ronald F. Renzi

    发明人： Ronald F. Renzi

    IPC分类号： B01L99/00

    CPC分类号： F16L39/00 ,  B01L3/565 ,  B01L2200/027 ,  B01L2300/0838 ,  G01N2030/185

    摘要： An interconnecting device for connecting a plurality of first fluid-bearing conduits to a corresponding plurality of second fluid-bearing conduits thereby providing fluid communication between the first fluid-bearing conduits and the second fluid-bearing conduits. The device includes a manifold and one or two ferrule plates that are held by compressive axial forces.

    摘要翻译： 一种互连装置，用于将多个第一流体承载管道连接到相应的多个第二流体承载导管，从而在第一流体承载管道和第二流体承载导管之间提供流体连通。 该装置包括通过压缩轴向力保持的歧管和一个或两个套圈板。

    MICROELECTROPORATION DEVICE FOR GENOMIC SCREENING
    10.
    发明申请
    MICROELECTROPORATION DEVICE FOR GENOMIC SCREENING 有权
    用于基因检测的微电子设备

    公开(公告)号：US20120004144A1

    公开(公告)日：2012-01-05

    申请号：US13173180

    申请日：2011-06-30

    申请人： Thomas D. Perroud ,  Ronald F. Renzi ,  Oscar Negrete ,  Mark R. Claudnic

    发明人： Thomas D. Perroud ,  Ronald F. Renzi ,  Oscar Negrete ,  Mark R. Claudnic

    IPC分类号： C40B60/12

    CPC分类号： B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0645 ,  B01L2300/0864 ,  C12M35/02 ,  Y10T436/2575

    摘要： We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.

    摘要翻译： 我们开发了一种微电极装置，其结合寡核苷酸微阵列，微流体通道和电穿孔用于细胞转染和高通量筛选应用（例如RNA干扰筛选）。 微阵列允许在微观斑点中沉积数千种不同的寡核苷酸。 微流体通道和微孔能够有效地将细胞装载到装置中并防止不同寡核苷酸斑点之间的交叉污染。 电穿孔允许通过最大限度地减少细胞死亡同时使转染效率最大化将核酸最佳地转染到细胞（特别是难转染细胞，如原代细胞）中。 本发明具有较高的生产能力和较低的成本，同时防止与常规筛选技术相比的交叉污染。 此外，该装置不需要庞大的机器人液体处理设备，并且由于它是封闭的系统，所以固有地更安全。