公开(公告)号：US20070158195A1

公开(公告)日：2007-07-12

申请号：US11714222

申请日：2007-03-06

申请人： Ryoji Inaba ,  Tomohiro Shoji ,  Shozo Kasai ,  Masaya Kojima ,  Yasushi Shimizu ,  Seiichi Ugai ,  Eric Nordman

发明人： Ryoji Inaba ,  Tomohiro Shoji ,  Shozo Kasai ,  Masaya Kojima ,  Yasushi Shimizu ,  Seiichi Ugai ,  Eric Nordman

IPC分类号： G01N27/00

CPC分类号： G01N27/44782 ,  G01N27/44721

摘要： An electrophoresis apparatus includes a multi-capillary array having a liquid or solid disposed between the capillaries of the array. The liquid or solid exhibits a refractive index higher than that of air and less than that of water and reduces the amount of laser beams scattered by the capillaries. Also provided are methods of adjusting refracted and reflected excitation light beams passing through capillaries of a multi-capillary array, to reduce loss of intensity of the laser beams and increase irradiation of respective samples disposed in the capillaries.

公开(公告)号：US20070175761A1

公开(公告)日：2007-08-02

申请号：US11714221

申请日：2007-03-06

申请人： Ryoji Inaba ,  Tomohiro Shoji ,  Shozo Kasai ,  Masaya Kojima ,  Yasushi Shimizu ,  Selichi Ugai ,  Eric Nordman

发明人： Ryoji Inaba ,  Tomohiro Shoji ,  Shozo Kasai ,  Masaya Kojima ,  Yasushi Shimizu ,  Selichi Ugai ,  Eric Nordman

IPC分类号： G01N27/00

CPC分类号： G01N27/44782 ,  G01N27/44721

摘要： An electrophoresis apparatus includes a multi-capillary array having a liquid or solid disposed between the capillaries of the array. The liquid or solid exhibits a refractive index higher than that of air and less than that of water and reduces the amount of laser beams scattered by the capillaries. Also provided are methods of adjusting refracted and reflected excitation light beams passing through capillaries of a multi-capillary array, to reduce loss of intensity of the laser beams and increase irradiation of respective samples disposed in the capillaries.

公开(公告)号：US20070053186A1

公开(公告)日：2007-03-08

申请号：US11356875

申请日：2006-02-16

申请人： Richard Reel ,  Eric Nordman ,  Tomoyuki Sakai ,  Satoshi Takahashi ,  Ryoji Inaba

发明人： Richard Reel ,  Eric Nordman ,  Tomoyuki Sakai ,  Satoshi Takahashi ,  Ryoji Inaba

IPC分类号： F21S6/00

CPC分类号： G01N21/64 ,  F21V5/00 ,  G01N21/645 ,  G01N27/44721 ,  G01N2021/6463 ,  G01N2201/068 ,  Y10T436/143333

摘要： System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.

摘要翻译： 用于荧光灯激发和检测样品的系统和方法，以提高数值孔径和/或减少荧光灯的串扰。

公开(公告)号：US08102529B2

公开(公告)日：2012-01-24

申请号：US12637717

申请日：2009-12-14

申请人： Richard Reel ,  Eric Nordman ,  Tomoyuki Sakai ,  Satoshi Takahashi ,  Ryoji Inaba

发明人： Richard Reel ,  Eric Nordman ,  Tomoyuki Sakai ,  Satoshi Takahashi ,  Ryoji Inaba

IPC分类号： G01J4/00

CPC分类号： G01N21/64 ,  F21V5/00 ,  G01N21/645 ,  G01N27/44721 ,  G01N2021/6463 ,  G01N2201/068 ,  Y10T436/143333

摘要： System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.

摘要翻译： 用于荧光灯激发和检测样品的系统和方法，以提高数值孔径和/或减少荧光灯的串扰。

公开(公告)号：US08446588B2

公开(公告)日：2013-05-21

申请号：US13465999

申请日：2012-05-07

申请人： Richard Reel ,  Eric Nordman

发明人： Richard Reel ,  Eric Nordman

IPC分类号： G01N21/00 ,  G01N21/55

CPC分类号： G01N21/64 ,  F21V5/00 ,  G01N21/645 ,  G01N27/44721 ,  G01N2021/6463 ,  G01N2201/068 ,  Y10T436/143333

摘要： System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.

摘要翻译： 用于荧光灯激发和检测样品的系统和方法，以提高数值孔径和/或减少荧光灯的串扰。

公开(公告)号：US08384899B2

公开(公告)日：2013-02-26

申请号：US13442143

申请日：2012-04-09

申请人： Mark Oldham ,  Eric Nordman ,  Richard Reel ,  Janice Shigeura

发明人： Mark Oldham ,  Eric Nordman ,  Richard Reel ,  John Shigeura

IPC分类号： G01N21/00

CPC分类号： G01N27/44721 ,  B82Y10/00 ,  B82Y20/00 ,  G01N2015/149 ,  G01N2015/1497

摘要： An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.

公开(公告)号：US20070068573A1

公开(公告)日：2007-03-29

申请号：US11508756

申请日：2006-08-22

申请人： David Cox ,  Sam Woo ,  Steven Boege ,  Mark Oldham ,  Richard Reel ,  Eric Nordman ,  Linda Lee ,  Willy Wiyatno ,  Dmitry Sagatelyan

发明人： David Cox ,  Sam Woo ,  Steven Boege ,  Mark Oldham ,  Richard Reel ,  Eric Nordman ,  Linda Lee ,  Willy Wiyatno ,  Dmitry Sagatelyan

IPC分类号： F17D1/00

CPC分类号： C12Q1/6869 ,  B01J2219/00353 ,  B01J2219/0036 ,  B01J2219/00364 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/0293 ,  B01L3/502715 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0421 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q2535/101 ,  F15C5/00 ,  F16K99/0001 ,  F16K99/0011 ,  F16K99/0013 ,  F16K2099/0084 ,  G01N1/14 ,  G01N27/44743 ,  G01N27/44769 ,  G01N35/08 ,  Y10T137/0318 ,  Y10T137/4259 ,  Y10T137/85978 ,  Y10T137/85986 ,  Y10T137/86863 ,  Y10T436/2575

摘要： Various embodiments described in the application relate to an apparatus, system, and method for fluidically controlling, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise combinations of detectors, controllers, valves, and fluid supply units to control the spatial size, location and/or fluidic composition of discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.

摘要翻译： 在本申请中描述的各种实施例涉及用于在管道内流体控制与第二流体不混溶的一种或多种流体的离散体积的装置，系统和方法。 离散体积可用于涉及小体积的生物化学或分子生物学过程，例如微升体积，纳升级体积或更小体积。 该系统可以包括检测器，控制器，阀和流体供应单元的组合，以控制通过与离散的流体不混溶的流体彼此分离的离散体积的空间尺寸，位置和/或流体组成 体积，例如由油分离的含水不混溶流体离散体积。

公开(公告)号：US08384898B2

公开(公告)日：2013-02-26

申请号：US13442107

申请日：2012-04-09

申请人： Mark Oldham ,  Eric Nordman ,  Richard Reel ,  Janice Shigeura

发明人： Mark Oldham ,  Eric Nordman ,  Richard Reel ,  John Shigeura

IPC分类号： G01N21/00

CPC分类号： G01N27/44721 ,  B82Y10/00 ,  B82Y20/00 ,  G01N2015/149 ,  G01N2015/1497

摘要： An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.

摘要翻译： 提供了一种用于检测标记珠粒的装置。 该装置可以包括：一个或多个辐射源，被设置用于用辐射照射一个或多个检测区域; 至少一个检测器被设置用于收集对应于已经被辐射激发的一个或多个检测区域中的标记珠子发射的光信号的电荷; 以及耦合到所述至少一个检测器的系统，用于通过在读取至少一个检测器的输出端的电荷之前累积电荷来实现电荷的时间延迟积分。

公开(公告)号：US20060187450A1

公开(公告)日：2006-08-24

申请号：US11356497

申请日：2006-02-16

申请人： Richard Reel ,  Eric Nordman

发明人： Richard Reel ,  Eric Nordman

IPC分类号： G01N21/00

CPC分类号： G01N21/64 ,  F21V5/00 ,  G01N21/645 ,  G01N27/44721 ,  G01N2021/6463 ,  G01N2201/068 ,  Y10T436/143333

摘要： System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.

公开(公告)号：US20140045701A1

公开(公告)日：2014-02-13

申请号：US13824129

申请日：2011-10-04

申请人： Hesaam Esfandyarpour ,  Mark F. Oldham ,  Kosar Parazi ,  Eric Nordman

发明人： Hesaam Esfandyarpour ,  Mark F. Oldham ,  Kosar Parazi ,  Eric Nordman

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0655 ,  B03C1/286 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B60/10 ,  G01N1/40 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  G01N2001/4038 ,  H01L29/00 ,  C12Q2523/307 ,  C12Q2563/143 ,  C12Q2565/543 ,  C12Q2563/116 ,  C12Q2565/607

摘要： A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.

摘要翻译： 一种方法包括在衬底的特定位置磁性地保持携带生物材料（例如，核酸，其可以是DNA片段或扩增的DNA的形式）的珠，并施加局部于珠的电场以分离生物 材料或生物材料的反应产物或副产物。 例如，珠粒与具有相关生物材料的其它珠粒分离。 各种实施方案中的电场浓缩用于扩增或测序反应的试剂，和/或浓缩和分离可检测的反应副产物。 例如，通过分离单个珠周围的核酸，电场可以允许克隆扩增，作为乳液PCR的替代。 在其他实施例中，电场将纳米传感器隔离到珠子附近，以便于检测局部pH变化，局部电导率变化，局部电荷浓度变化和局部热中的至少一种。 珠可以以局部磁场区域阵列的形式被捕获。