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22 条记录 (耗时 0.039 秒)

    Method for analytically utilizing microfabricated sensors during wet-up
    1.
    发明授权
    Method for analytically utilizing microfabricated sensors during wet-up 失效
    在润湿期间分析利用微波传感器的方法

    公开(公告)号：US5112455A

    公开(公告)日：1992-05-12

    申请号：US555976

    申请日：1990-07-20

    申请人： Stephen N. Cozzette ,  Graham Davis ,  Lisa A. Holleritter ,  Imants R. Lauks ,  Sylvia Piznik ,  Nicolaas J. Smit ,  Jody A. Tirinato ,  Henry J. Wieck ,  Michael P. Zelin

    发明人： Stephen N. Cozzette ,  Graham Davis ,  Lisa A. Holleritter ,  Imants R. Lauks ,  Sylvia Piznik ,  Nicolaas J. Smit ,  Jody A. Tirinato ,  Henry J. Wieck ,  Michael P. Zelin

    IPC分类号： G01N27/26 ,  G01N27/27 ,  G01N27/327 ,  G01N27/414 ,  G01N27/416 ,  G01N33/543

    CPC分类号： G01N27/3274

    摘要： A method of determining the concentration of a preselected analyte species present in a sample fluid is disclosed, which method comprises: (a) providing an external computational means, a reference electrode and at least one substantially dry-stored sensor capable of exhibiting a response to changes in the concentration of a preselected analyte species before the sensor attains full equilibrated wet-up; (b) establishing electrical contact between the sensor, reference electrode and external computational means; (c) contacting the sensor and reference electrode with a calibrant fluid; (d) performing a first signal measurement in a first time window in the presence of the calibrant fluid; (e) displacing the calibrant fluid after performing the first signal measurement; (f) contacting the sensor and reference electrode with a sample fluid; (g) performing a second signal measurement in a second time window in the presence of the sample fluid; and (h) relating the first and second signal measurements to determine the concentration of the preselected analyte species in the sample fluid before said sensor attains full equilibrated wet-up. Alternative methods are disclosed further for collecting, relating and manipulating the results of the signal measurements to provide useful estimates of the analyte concentrations of interest.

    摘要翻译： 公开了一种确定存在于样品流体中的预先选择的分析物种的浓度的方法，该方法包括：（a）提供外部计算装置，参考电极和至少一个基本上干燥储存的传感器，其能够显示对 在传感器达到完全平衡润湿之前，预选的分析物种的浓度变化; （b）建立传感器，参比电极和外部计算装置之间的电接触; （c）使传感器和参比电极与校准液接触; （d）在存在校准液的情况下在第一时间窗中执行第一信号测量; （e）在执行第一信号测量之后移动校准液; （f）使传感器和参比电极接触样品流体; （g）在存在样品流体的情况下在第二时间窗中执行第二信号测量; 和（h）将第一和第二信号测量值相关联，以确定在所述传感器达到完全平衡的润湿之前样品流体中预先选择的分析物种的浓度。 进一步披露替代方法来收集，相关和操纵信号测量结果，以提供感兴趣的分析物浓度的有用估计。

    Methods for microdispensing patterened layers
    3.
    发明授权
    Methods for microdispensing patterened layers 失效
    微分配图案的方法

    公开(公告)号：US06306594B1

    公开(公告)日：2001-10-23

    申请号：US09193370

    申请日：1998-11-17

    申请人： Stephen N. Cozzette ,  Graham Davis ,  Imants R. Lauks ,  Randall M. Mier ,  Sylvia Piznik ,  Nicolaas Smit ,  Paul Van der Werf ,  Henry J. Wieck

    发明人： Stephen N. Cozzette ,  Graham Davis ,  Imants R. Lauks ,  Randall M. Mier ,  Sylvia Piznik ,  Nicolaas Smit ,  Paul Van der Werf ,  Henry J. Wieck

    IPC分类号： G01N3350

    CPC分类号： B01L3/5027 ,  B01L3/0268 ,  B01L3/502707 ,  B01L3/5088 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0819 ,  B01L2300/089 ,  C12Q1/001 ,  G01N33/5438 ,  G01N35/0099 ,  G01N35/109 ,  G01N2035/00059 ,  G01N2035/00237 ,  G01N2035/00455 ,  G01N2035/00534 ,  G01N2035/1034 ,  G01N2035/1076 ,  Y10S435/81 ,  Y10S436/809

    摘要： An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments. The present invention also discloses a novel method for the electrochemical detection of particular analyte species of biological and physiological significance using an substrate/label signal generating pair which produces a change in the concentration of electroactive species selected from the group consisting of dioxygen and hydrogen peroxide.

    摘要翻译： 描述了适用于分析生物学重要的分析物种的电子装置的微细加工的有效方法。 本发明的技术允许对在合适的衬底上建立的各种组件和层的尺寸特征进行密切的控制。 这种控制延伸到结合生物组分的装置的那些部分，使得这些装置能够用作生物传感器。 因此，本文公开的材料和方法提供了用于批量生产均匀的全微生物传感器的有效手段。 本文描述了装置本身的各种实施例，其特别适用于临床环境中的生物样品的实时分析。 特别地，本发明描述了可以使用某些基于配体/配体受体的生物传感器实施方案进行的测定。 本发明还公开了一种利用基板/标记信号产生对电化学检测特定分析物种的生物学和生理意义的新方法，其产生选自二氧和过氧化氢的电活性物质浓度的变化。

    Disposable sensing device for real time fluid analysis
    10.
    发明授权
    Disposable sensing device for real time fluid analysis 失效
    用于实时流体分析的便携式感应装置

    公开(公告)号：US5096669A

    公开(公告)日：1992-03-17

    申请号：US245102

    申请日：1988-09-15

    申请人： Imants R. Lauks ,  Henry J. Wieck ,  Michael P. Zelin ,  Philip Blyskal

    发明人： Imants R. Lauks ,  Henry J. Wieck ,  Michael P. Zelin ,  Philip Blyskal

    IPC分类号： G01N27/26 ,  B01L3/00 ,  G01N27/07 ,  G01N27/27 ,  G01N27/28 ,  G01N33/487 ,  G01N33/49

    CPC分类号： B01L3/502715 ,  B01L3/502707 ,  B01L2300/0672 ,  B01L2300/087 ,  B01L2400/0481 ,  B01L2400/0683

    摘要： A system comprising a disposable device and hand held reader can perform a variety of electrochemical measurements on blood or other fluids. In operation, a fluid sample is drawn into the disposable device through an orifice by capillary action. The orifice is sealed off and the disposable device is inserted into the reader. The reader which controls the test sequence and flow of fluid causes a calibrant pouch located inside the device to be pierced, releasing the calibrant fluid to flow across the sensor arrays to perform calibration. Next an air bladder located in the device is depressed, forcing the sample across the sensors where measurements are performed and read by the reader which performs the calibrations. Once the measurements are made, the device can be withdrawn from the reader and discarded.

    摘要翻译： 包括一次性装置和手持读取器的系统可对血液或其它流体执行各种电化学测量。 在操作中，流体样品通过毛细作用通过孔被吸入一次性装置。 密封孔，将一次性装置插入读取器。 控制流体的测试顺序和流量的读取器导致位于设备内部的校准物袋被刺穿，释放校准液流过传感器阵列以进行校准。 接下来，位于设备中的气囊被压下，迫使样本穿过传感器，在那里进行测量并由执行校准的读取器读取。 一旦进行测量，设备可以从读取器中取出并丢弃。