摘要:
A film winding device for slide projectors of a type using strip films comprising a film housing case capable of being removably fitted to a projector body, a film guide guiding a projected film toward the film housing case, a leading ring mounted rotatably on the end portion of the film guide and capable of leading in the projected film at the tip so as to come to the outside within the film housing case and a roller means arranged so as to be able to positively feed the projected film even in the end portion into the film housing case, whereby the film having finished being projected can be housed successively into the film housing case so as to be projectable without being rewound. In this slide projector, the film to be projected can be easily taken at the tip to the position of the film guide by pushing a button.
摘要:
A purpose of the present invention is to provide a gene amplification apparatus with simple operation, wherein the apparatus can heat and cool gene rapidly and a thermal cycle can be operated in a relatively short period and a method thereof, a method for preparing immobilized DNA library suitable to the above apparatus and a method for comparing genes systematically. The apparatus for preparing immobilized DNA library according to the present invention comprises a reaction body 10 on which a grooved portion for receiving a container is formed, a cap portion 50 provided at an upper portion of the reaction body and a container 12 formed by substrates for immobilizing DNA, wherein the cap portion 50 including means for heating/cooling 51 and means for cooling 52.
摘要:
A bond substrate is attached with an incline toward the setting surface of a base substrate. Accordingly, an attachment starting portion can be limited. Further, the bond substrate is provided so that part of the bond substrate extends beyond a support base and the part is closest to the base substrate. Because of this, part of the bond substrate is separated from the support base with the use of an end portion of the support base as a fulcrum point because the support base is not provided below the contact portion, and attachment sequentially proceeds from a portion which gets close to the base substrate; thus, stable attachment can be performed without an air layer remaining at the interface between the bond substrate and the base substrate.
摘要:
Substrates for producing a DNA library are produced by immobilizing DNA onto a suitable substrate having excellent thermal conductivity. A surface of the substrate is chemically modified with a radical having a terminal polar radical. DNA can be amplified by the PCR method in a short period of time using substrates of the present invention onto which DNA is immobilized.
摘要:
It is intended to provide a method of reusing a DNA-immobilization substrate whereby the expensive DNA-immobilization substrate can be efficiently utilized and a reusable DNA-immobilization substrate having the same performance as a new product without any trouble in practical use can be provided. Namely, a method of reusing a DNA-immobilization substrate characterized in that, to remove DNA from a DNA-immobilization substrate carrying the DNA immobilized by an acid-amide bond via an oligonucleotide to thereby enable the immobilization of a fresh DNA, the acid-amide bond between the substrate and the DNA is hydrolyzed with an acid or an alkali.
摘要:
A support for fixing a nucleotide that contributes to the efficient clarification of DNA without damaging the terminal parts of the DNA and, therefore, is useful in the fields of biology, biochemistry and the like. This support for fixing a nucleotide is a chemically modified substrate on which oligonucleotide are immobilized characterized in that the oligonucleotide have a restriction enzyme cleavage site. This support is produced by chemically modifying the substrate, immobilizing a single-stranded oligonucleotide thereon, then hybridizing the single-stranded oligonucleotide with another single-stranded oligonucleotide having a base sequence complementary thereto, and ligating an oligonucleotide having a restriction enzyme site at the end thereof.
摘要:
An electrostatic chuck including an electrode having first and second surfaces, a dielectric member having a first layer for covering at least the first surface of the electrode, a power supply for conducting electric power to the electrode, and a cooling gas-feeding means for feeding a cooling gas onto the surface of the first layer of the dielectric member. A plurality of fine projections 28 are formed on the surface of the first layer of the dielectric member. Each of the fine projections 28 has a tip smaller than a root thereof so as to hold a wafer W in substantially a point-contact manner. The wafer can be held on the surface of the first layer of the dielectric member and cooled by the cooling gas fed from the cooling gas-feeding means.
摘要:
Method and apparatus for constructing a cDNA library by hybridizing mRNA with oligo (dT) on a support and treating with a reverse transcriptase to immobilized complementary DNA, or for constructing a gDNA library by ligating a double-stranded chromosomal DNA library with an oligonucleotide on a support having a restriction enzyme site and then immobilizing the gDNA library.
摘要:
A subject of the present invention is to provide a support with immobilized DNA as a DNA library and particularly to a support suitable for reproducing DNA in accordance with a DNA amplification reaction. In the present invention, DNA or the like is immobilized on a surface of a support made of one kind or more than material Selected from a group of a diamond including non-diamond carbon, amorphous carbon and graphite. A hydroxyl group may be bonded to a surface of the support or a group bonded to a hydrocarbon group and a carboxyl group at its terminal end may be bonded to a surface of the support through an ester linkage or a peptide linkage.