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    Sorting device for biological cells or viruses
    5.
    发明授权
    Sorting device for biological cells or viruses 失效
    用于生物细胞或病毒的分选装置

    公开(公告)号：US5837200A

    公开(公告)日：1998-11-17

    申请号：US653143

    申请日：1996-05-24

    申请人： Edgar Diessel ,  Walter Weichel

    发明人： Edgar Diessel ,  Walter Weichel

    IPC分类号： C12M1/00 ,  B01L3/00 ,  B03C1/035 ,  C12M1/42 ,  C12Q1/04 ,  C12Q1/24 ,  C12Q1/70 ,  F15C1/08 ,  G01N15/14 ,  G01N33/569 ,  G01N33/00

    CPC分类号： F15C1/08 ,  B01L3/502761 ,  B03C1/035 ,  B01L2200/0647 ,  B01L2300/0864 ,  B01L2400/043 ,  B01L2400/0439 ,  G01N2015/149 ,  Y10S209/906 ,  Y10S435/808

    摘要： The sorting device is able to sort biological objects, particularly cells or viruses, in liquids as a function of physically measurable criteria or properties. The sorting apparatus is provided with a feed inlet (1) for the biological liquid which branches into a microstructured system of multi-parallel main channels (3) and sorting modules (4) each with a switch unit for distribution to two different outlet channels (19,20). Inside a sorting module (4), at least one sensor (9,10) is arranged on each main channel, and a sorting actuator (12) controlled by the relevant sensor is arranged on each switch unit (11). Finally, each outlet channel (20) is connected to a collecting channel (6) for the unselected biological cells or viruses, and each outlet channel (19) is connected to a collecting channel (5) for the selected cells or viruses. If the cells or viruses are magnetically marked, it is possible to dispense with the sensor (9,10) on the main channels (3). In that case, the sorting actuators arranged on the switch units are designed as magnetic deflection modules (37,38). In this case, the magnetic deflection modules undertake direct classification or selection as a function of the magnetic properties of the biological objects to be sorted.

    摘要翻译： 分选装置能够根据物理上可测量的标准或性质将生物体，特别是细胞或病毒分离在液体中。 分拣装置设置有用于生物液体的进料口（1），其分支成多平行主通道（3）和分拣模块（4）的微结构化系统，每个分配模块具有开关单元，用于分配到两个不同的出口通道 19,20）。 在分拣模块（4）内，在每个主通道上布置有至少一个传感器（9,10），并且在每个开关单元（11）上布置由相关传感器控制的分拣机构（12）。 最后，每个出口通道（20）连接到用于未选择的生物细胞或病毒的收集通道（6），并且每个出口通道（19）连接到用于所选细胞或病毒的收集通道（5）。 如果细胞或病毒被磁性标记，则可以省去主通道（3）上的传感器（9,10）。 在这种情况下，布置在开关单元上的排序致动器被设计为磁偏转模块（37,38）。 在这种情况下，磁偏转模块根据要分选的生物体的磁特性进行直接分类或选择。

    Process and device for the screening of molecules with regard to their individual binding behaviour towards at least one given ligand
    7.
    发明授权
    Process and device for the screening of molecules with regard to their individual binding behaviour towards at least one given ligand 失效
    关于它们对至少一个给定配体的单独结合行为的分子筛选的方法和装置

    公开(公告)号：US06713264B2

    公开(公告)日：2004-03-30

    申请号：US08891778

    申请日：1997-07-14

    申请人： Klaus Luttermann ,  Edgar Diessel ,  Winfried Kosch ,  Walter Weichel

    发明人： Klaus Luttermann ,  Edgar Diessel ,  Winfried Kosch ,  Walter Weichel

    IPC分类号： G01N3353

    CPC分类号： C12M47/04 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/0072 ,  G01N15/1468 ,  Y10S435/808

    摘要： The process is used for the screening of molecules from molecule libraries with regard to their individual binding behavior towards at least one given ligand. For this purpose the ligands labelled with a fluorescent dye are mixed with the molecule library which is in the form of a suspension. The mixture is plated out on a two-dimensional substrate (2) after the excess, unbound ligands have been washed out. Then the local fluorescence intensities on the substrate are electro-optically identified in a fluorescence microscope (5) and electronically discriminated in accordance with given selection criteria. The objects selected and localised in this way are then sequentially positioned exactly by a displacement, the coordinates of which are controlled by the image calculator, between the substrate (2) and a separation actuator (20, 21) and are spatially separated from the substrate (2) by the separation actuator (20, 21).

    摘要翻译： 该方法用于从分子文库筛选针对至少一种给定配体的单个结合行为的分子。 为此，用荧光染料标记的配体与悬浮液形式的分子文库混合。 在过量的未结合配体被洗掉之后，将混合物镀在二维底物（2）上。 然后在荧光显微镜（5）中对基底上的局部荧光强度进行电光学鉴定，并根据给定的选择标准进行电子鉴别。 以这种方式选择和定位的物体然后通过基板（2）和分离致动器（​​20,21）之间的位移（其坐标由图像计算器控制）精确地顺序地定位并且在空间上与基板分离 （2）通过分离致动器（​​20,21）。