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    METHOD FOR DETECTING THE PRESENCE OF A TARGET ANALYTE IN A TEST SPOT
    3.
    发明申请
    METHOD FOR DETECTING THE PRESENCE OF A TARGET ANALYTE IN A TEST SPOT 审中-公开
    用于检测目标分析仪在测试点中的存在的方法

    公开(公告)号：US20120063653A1

    公开(公告)日：2012-03-15

    申请号：US12853047

    申请日：2010-08-09

    申请人： William Cork ,  Tim Patno ,  Mark Weber ,  Dave Morrow ,  Wesley Buckingham

    发明人： William Cork ,  Tim Patno ,  Mark Weber ,  Dave Morrow ,  Wesley Buckingham

    IPC分类号： G06K9/00

    CPC分类号： G01N33/54346 ,  G01N21/253

    摘要： An apparatus and method for imaging metallic nanoparticles is provided. Preferably, the invention provides for an apparatus and method for detection of gold colloid particles and for accurate reporting to the operator. The apparatus includes a substrate holder for holding the substrate, a processor and memory device, an imaging module, an illumination module, a power module, an input module, and an output module. The apparatus may have a stationary substrate holder and imaging module which are proximate to one another. The apparatus provided for a compact sized system without the need for complex motorized devices to move the camera across the substrate. Further, the apparatus and method provide for automatic detection of the spots/wells on the substrate, automatic quantification of the spots on the substrate, and automatic interpretation of the spots based on decision statistics.

    摘要翻译： 提供了一种用于成像金属纳米颗粒的设备和方法。 优选地，本发明提供了用于检测金胶体颗粒并且准确地向操作者报告的装置和方法。 该装置包括用于保持基板的基板支架，处理器和存储装置，成像模块，照明模块，电源模块，输入模块和输出模块。 该装置可以具有彼此靠近的固定衬底保持器和成像模块。 该设备提供了一种紧凑型系统，而不需要复杂的电动装置来使相机移动穿过基板。 此外，该装置和方法提供对基板上的点/孔的自动检测，基板上的点的自动定量，以及基于判定统计的斑点的自动解释。

    Method of preparing nucleic acids for detection
    4.
    发明授权
    Method of preparing nucleic acids for detection 有权
    制备核酸检测方法

    公开(公告)号：US07396677B2

    公开(公告)日：2008-07-08

    申请号：US10703368

    申请日：2003-11-07

    申请人： Tim Patno ,  Jennifer Hollenstein ,  Christian Kronshage ,  Christopher Khoury ,  Mark Weber ,  Tom Westberg ,  William Cork

    发明人： Tim Patno ,  Jennifer Hollenstein ,  Christian Kronshage ,  Christopher Khoury ,  Mark Weber ,  Tom Westberg ,  William Cork

    IPC分类号： C12M1/34 ,  C12M3/00 ,  C12M1/22 ,  B01L3/00 ,  B01L3/02

    CPC分类号： C12Q1/6806 ,  B01L3/5025 ,  B01L3/5027 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/0877 ,  B01L2400/0487 ,  G01N35/1097 ,  C12Q2563/155 ,  C12Q2565/519

    摘要： A method and apparatus are provided for preparing a test sample for detecting a predetermined target nucleic acid. The method includes the steps of providing a test probe comprising an oligonucleotide attached to a nanoparticle and providing a hybridization unit containing the test sample and the test probe, wherein said hybridization unit further includes a target sample substrate and a distribution manifold coupled to a first side of the substrate. The method further includes the steps of clamping a processing fluids manifold to the distribution manifold of the hybridization unit, denaturing the test sample and preparing the test sample for detecting the predetermined target nucleic acid by pumping a plurality of processing fluids between the processing fluids source manifold and distribution manifold to hybridize the test probe and predetermined target nucleic acid to the target sample substrate, to wash the hybridized sample and to amplify a detectable parameter of the hybridized sample.

    摘要翻译： 提供了一种制备用于检测预定靶核酸的测试样品的方法和装置。 该方法包括以下步骤：提供包含与纳米颗粒连接的寡核苷酸并提供含有测试样品和测试探针的杂交单元的测试探针，其中所述杂交单元还包括目标样品基底和连接到第一侧的分布歧管 的基底。 该方法还包括以下步骤：将加工流体歧管夹紧到杂交单元的分配歧管上，使测试样品变性并制备用于通过在处理流体源歧管之间泵送多个处理流体来检测预定靶核酸的测试样品 以及分配多肽以将测试探针和预定靶核酸与目标样品底物杂交，以洗涤杂交样品并扩增杂交样品的可检测参数。

    Method of preparing nucleic acids for detection
    5.
    发明申请
    Method of preparing nucleic acids for detection 有权
    制备核酸检测方法

    公开(公告)号：US20050112583A1

    公开(公告)日：2005-05-26

    申请号：US10703368

    申请日：2003-11-07

    申请人： Tim Patno ,  Jennifer Hollenstein ,  Christian Kronshage ,  Christopher Khoury ,  Mark Weber ,  Tom Westberg ,  William Cork

    发明人： Tim Patno ,  Jennifer Hollenstein ,  Christian Kronshage ,  Christopher Khoury ,  Mark Weber ,  Tom Westberg ,  William Cork

    IPC分类号： B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  C12M1/34 ,  C12Q1/68 ,  G01N20060101 ,  G01N35/10

    CPC分类号： C12Q1/6806 ,  B01L3/5025 ,  B01L3/5027 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/0877 ,  B01L2400/0487 ,  G01N35/1097 ,  C12Q2563/155 ,  C12Q2565/519

    摘要： A method and apparatus are provided for preparing a test sample for detecting a predetermined target nucleic acid. The method includes the steps of providing a test probe comprising an oligonucleotide attached to a nanoparticle and providing a hybridization unit containing the test sample and the test probe, wherein said hybridization unit further includes a target sample substrate and a distribution manifold coupled to a first side of the substrate. The method further includes the steps of clamping a processing fluids manifold to the distribution manifold of the hybridization unit, denaturing the test sample and preparing the test sample for detecting the predetermined target nucleic acid by pumping a plurality of processing fluids between the processing fluids source manifold and distribution manifold to hybridize the test probe and predetermined target nucleic acid to the target sample substrate, to wash the hybridized sample and to amplify a detectable parameter of the hybridized sample.

    摘要翻译： 提供了一种制备用于检测预定靶核酸的测试样品的方法和装置。 该方法包括以下步骤：提供包含与纳米颗粒连接的寡核苷酸并提供含有测试样品和测试探针的杂交单元的测试探针，其中所述杂交单元还包括目标样品基底和连接到第一侧的分布歧管 的基底。 该方法还包括以下步骤：将加工流体歧管夹紧到杂交单元的分配歧管上，使测试样品变性并制备用于通过在处理流体源歧管之间泵送多个处理流体来检测预定靶核酸的测试样品 以及分配多肽以将测试探针和预定靶核酸与目标样品底物杂交，以洗涤杂交样品并扩增杂交样品的可检测参数。

    Method of Preparing Nucleic Acids for Detection
    7.
    发明申请
    Method of Preparing Nucleic Acids for Detection 审中-公开
    准备检测核酸的方法

    公开(公告)号：US20080268458A1

    公开(公告)日：2008-10-30

    申请号：US12169618

    申请日：2008-07-08

    申请人： Tim Patno ,  Jennifer Hollenstein ,  Christian Kronshage ,  Christopher Khoury ,  Mark Weber ,  Tom Westberg ,  William Cork

    发明人： Tim Patno ,  Jennifer Hollenstein ,  Christian Kronshage ,  Christopher Khoury ,  Mark Weber ,  Tom Westberg ,  William Cork

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6806 ,  B01L3/5025 ,  B01L3/5027 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/0877 ,  B01L2400/0487 ,  G01N35/1097 ,  C12Q2563/155 ,  C12Q2565/519

    摘要： A method is provided for preparing a test sample for detecting a predetermined target nucleic acid. The method includes the steps of providing a test probe comprising an oligonucleotide attached to a nanoparticle and providing a hybridization unit containing the test sample and the test probe, wherein said hybridization unit further includes a target sample substrate and a distribution manifold coupled to a first side of the substrate. The method further includes the steps of clamping a processing fluids manifold to the distribution manifold of the hybridization unit, denaturing the test sample and preparing the test sample for detecting the predetermined target nucleic acid by pumping a plurality of processing fluids between the processing fluids source manifold and distribution manifold to hybridize the test probe and predetermined target nucleic acid to the target sample substrate, to wash the hybridized sample and to amplify a detectable parameter of the hybridized sample.

    摘要翻译： 提供了一种制备用于检测预定靶核酸的测试样品的方法。 该方法包括以下步骤：提供包含与纳米颗粒连接的寡核苷酸并提供含有测试样品和测试探针的杂交单元的测试探针，其中所述杂交单元还包括目标样品基底和连接到第一侧的分布歧管 的基底。 该方法还包括以下步骤：将加工流体歧管夹紧到杂交单元的分配歧管上，使测试样品变性并制备用于通过在处理流体源歧管之间泵送多个处理流体来检测预定靶核酸的测试样品 以及分配多肽以将测试探针和预定靶核酸与目标样品底物杂交，以洗涤杂交样品并扩增杂交样品的可检测参数。

    Method for automatically detecting spots on a substrate
    8.
    发明申请
    Method for automatically detecting spots on a substrate 审中-公开
    自动检测基板上的斑点的方法

    公开(公告)号：US20070148665A1

    公开(公告)日：2007-06-28

    申请号：US11530138

    申请日：2006-09-08

    申请人： William Cork ,  Tim Patno ,  Mark Weber ,  Dave Morrow ,  Wesley Buckingham

    发明人： William Cork ,  Tim Patno ,  Mark Weber ,  Dave Morrow ,  Wesley Buckingham

    IPC分类号： C12Q1/68 ,  G06T7/00

    CPC分类号： G01N33/54346 ,  G01N21/253

    摘要： An apparatus and method for imaging metallic nanoparticles is provided. Preferably, the invention provides for an apparatus and method for detection of gold colloid particles and for accurate reporting to the operator. The apparatus includes a substrate holder for holding the substrate, a processor and memory device, an imaging module, an illumination module, a power module, an input module, and an output module. The apparatus may have a stationary substrate holder and imaging module which are proximate to one another. The apparatus provided for a compact sized system without the need for complex motorized devices to move the camera across the substrate. Further, the apparatus and method provide for automatic detection of the spots/wells on the substrate, automatic quantification of the spots on the substrate, and automatic interpretation of the spots based on decision statistics.

    摘要翻译： 提供了一种用于成像金属纳米颗粒的设备和方法。 优选地，本发明提供了用于检测金胶体颗粒并且准确地向操作者报告的装置和方法。 该装置包括用于保持基板的基板支架，处理器和存储装置，成像模块，照明模块，电源模块，输入模块和输出模块。 该装置可以具有彼此靠近的固定衬底保持器和成像模块。 该设备提供了一种紧凑型系统，而不需要复杂的电动装置来使相机移动穿过基板。 此外，该装置和方法提供对基板上的点/孔的自动检测，基板上的点的自动定量，以及基于判定统计的斑点的自动解释。

    Method for Detecting the Presence of a Target Analyte in a Test Spot
    9.
    发明申请
    Method for Detecting the Presence of a Target Analyte in a Test Spot 有权
    检测目标分析物在测试点中的存在方法

    公开(公告)号：US20070041624A1

    公开(公告)日：2007-02-22

    申请号：US11530110

    申请日：2006-09-08

    申请人： William Cork ,  Tim Patno ,  Mark Weber ,  Dave Morrow ,  Wesley Buckingham

    发明人： William Cork ,  Tim Patno ,  Mark Weber ,  Dave Morrow ,  Wesley Buckingham

    IPC分类号： G06K9/00

    CPC分类号： G01N33/54346 ,  G01N21/253

    摘要： An apparatus and method for imaging metallic nanoparticles is provided. Preferably, the invention provides for an apparatus and method for detection of gold colloid particles and for accurate reporting to the operator. The apparatus includes a substrate holder for holding the substrate, a processor and memory device, an imaging module, an illumination module, a power module, an input module, and an output module. The apparatus may have a stationary substrate holder and imaging module which are proximate to one another. The apparatus provided for a compact sized system without the need for complex motorized devices to move the camera across the substrate. Further, the apparatus and method provide for automatic detection of the spots/wells on the substrate, automatic quantification of the spots on the substrate, and automatic interpretation of the spots based on decision statistics.

    摘要翻译： 提供了一种用于成像金属纳米颗粒的设备和方法。 优选地，本发明提供了用于检测金胶体颗粒并且准确地向操作者报告的装置和方法。 该装置包括用于保持基板的基板支架，处理器和存储装置，成像模块，照明模块，电源模块，输入模块和输出模块。 该装置可以具有彼此靠近的固定衬底保持器和成像模块。 该设备提供了一种紧凑型系统，而不需要复杂的电动装置来使相机移动穿过基板。 此外，该装置和方法提供对基板上的点/孔的自动检测，基板上的点的自动定量，以及基于判定统计的斑点的自动解释。

    Nanoparticle imaging system and method
    10.
    发明授权
    Nanoparticle imaging system and method 有权

    公开(公告)号：US07110585B2

    公开(公告)日：2006-09-19

    申请号：US10210959

    申请日：2002-08-02

    申请人： William Cork ,  Tim Patno ,  Mark Weber ,  Dave Morrow ,  Wesley Buckingham

    发明人： William Cork ,  Tim Patno ,  Mark Weber ,  Dave Morrow ,  Wesley Buckingham

    IPC分类号： G06K9/00

    CPC分类号： G01N33/54346 ,  G01N21/253

    摘要： An apparatus and method for imaging metallic nanoparticles is provided. Preferably, the invention provides for an apparatus and method for detection of gold colloid particles and for accurate reporting to the operator. The apparatus includes a substrate holder for holding the substrate, a processor and memory device, an imaging module, an illumination module, a power module, an input module, and an output module. The apparatus may have a stationary substrate holder and imaging module which are proximate to one another. The apparatus provided for a compact sized system without the need for complex motorized devices to move the camera across the substrate. Further, the apparatus and method provide for automatic detection of the spots/wells on the substrate, automatic quantification of the spots on the substrate, and automatic interpretation of the spots based on decision statistics.