公开(公告)号：US20240241024A1

公开(公告)日：2024-07-18

申请号：US18436262

申请日：2024-02-08

Applicant: ChemoMetec A/S

Inventor： Johan Holm

IPC: G01N15/0227 ,  G01N15/02 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1433 ,  G01N15/1434 ,  G02B21/14

CPC classification number: G01N15/0227 ,  G01N15/1433 ,  G01N15/1434 ,  G01N15/147 ,  G02B21/14 ,  G01N2015/0294 ,  G01N2015/1006 ,  G01N2015/144 ,  G01N2015/1452 ,  G01N2015/1454 ,  G01N2015/1493

Abstract: A method for characterizing object(s) in a sample includes collecting transmitted, refracted, scattered, diffracted, and/or emitted light from the sample. The collected light formed on a sensor surface includes at least a first and second image of the object(s). For at least the first and second image, the collected light is modulated asymmetrically differently, or for at least a third and fourth image focal plane positions are different, or for at least a fifth image the collected light is modulated in at least two places differently compared to the surroundings, or for at least a sixth and seventh image of the object(s), the collected light is modulated asymmetrically differently and focal planes of the sixth and seventh image have different positions. The images are processed to characterize the objects(s).

公开(公告)号：US11933707B2

公开(公告)日：2024-03-19

申请号：US17048423

申请日：2019-04-17

Applicant: ChemoMetec A/S

Inventor： Johan Holm

IPC: G01N15/02 ,  G01N15/14 ,  G02B21/14 ,  G01N15/10

CPC classification number: G01N15/0227 ,  G01N15/1434 ,  G01N15/147 ,  G01N15/1475 ,  G02B21/14 ,  G01N2015/0294 ,  G01N2015/1006 ,  G01N2015/144 ,  G01N2015/1452 ,  G01N2015/1454 ,  G01N2015/1493

Abstract: A method for characterizing object(s) in a sample includes collecting transmitted, refracted, scattered, diffracted, and/or emitted light from the sample. The collected light formed on a sensor surface includes at least a first and second image of the object(s). For at least the first and second image, the collected light is modulated asymmetrically differently, or for at least a third and fourth image focal plane positions are different, or for at least a fifth image the collected light is modulated in at least two places differently compared to the surroundings, or for at least a sixth and seventh image of the object(s), the collected light is modulated asymmetrically differently and focal planes of the sixth and seventh image have different positions. The images are processed to characterize the objects(s).

公开(公告)号：US08154731B2

公开(公告)日：2012-04-10

申请号：US11596141

申请日：2005-05-17

Applicant: Börkur Arnvidarson ,  Hans Larsen

Inventor： Börkur Arnvidarson ,  Hans Larsen

IPC: G01B9/02 ,  G01J3/45 ,  G01J5/02

CPC classification number: G01J3/45 ,  G01J3/0202 ,  G01J3/453 ,  G01N21/35 ,  G01N21/3563 ,  G01N21/3577 ,  G01N21/359 ,  G01N2021/3595 ,  G01N2201/0691 ,  G01N2201/129

Abstract: The present invention offers an alternative strategy for the correlation of interference information to chemical and/or physical properties of a sample. This strategy can be implemented in a method and a system, which offer substantial technical and commercial advantages over state of the art techniques based on interference spectroscopy. The invention further provides a method for standardizing an interferometer, as well as a method and a system using the standardized interferometer.

Abstract translation: 本发明提供了用于将干扰信息与样品的化学和/或物理性质相关的替代策略。 该策略可以在与基于干涉光谱技术的现有技术相比提供实质的技术和商业优势的方法和系统中实现。 本发明还提供了一种用于标准化干涉仪的方法，以及使用该标准化干涉仪的方法和系统。

公开(公告)号：US08125643B2

公开(公告)日：2012-02-28

申请号：US11414839

申请日：2006-05-01

Applicant: Frans Ejner Ravn Hansen ,  Martin Glensbjerg ,  Borkur Arnvidarson ,  Jesper Myron Jeppesen

Inventor： Frans Ejner Ravn Hansen ,  Martin Glensbjerg ,  Borkur Arnvidarson ,  Jesper Myron Jeppesen

IPC: G01N21/00 ,  G01N33/48

CPC classification number: G01N15/1459 ,  B01L3/5027 ,  G01N15/1463 ,  G01N21/31 ,  G01N21/59 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/76 ,  G01N33/04 ,  G01N2015/025 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2021/5957 ,  G01N2021/6491

Abstract: The present invention relates to a method for the assessment of quantity and quality parameters of biological particles in a liquid analyte material. The method comprises applying a volume of a liquid sample to an exposing domain from which exposing domain electromagnetic signals from the sample in the domain can pass to the exterior, and exposing, onto an array of active detection elements such as CCD-elements, a spatial representation of electromagnetic signals having passed from the domain, the representation being detectable as an intensity by individual active detection elements, under conditions permitting processing of the intensities detected by the array of detection elements during the exposure in such a manner that representations of electromagnetic signals from the biological particles are identified as distinct from representations of electromagnetic signals from background signals. The size of the volume of the liquid sample is sufficiently large to permit the assessment of the quantity and quality parameters to fulfill a predetermined requirement to the statistical quality of the assessment based on substantially one exposure.

Abstract translation: 本发明涉及一种用于评估液体分析物质中生物颗粒的数量和质量参数的方法。 该方法包括将一定体积的液体样品施加到曝光区域，从该区域中的来自样品的曝光区域电磁信号可以从该区域传播到外部，并将其暴露于诸如CCD元件的主动检测元件阵列上，空间 在从暴露期间通过检测元件阵列检测到的强度的处理条件下，可以通过各个有源检测元件检测作为强度的表示作为强度的表示， 生物颗粒被识别为不同于来自背景信号的电磁信号的表示。 液体样品的体积大小足够大以允许评估数量和质量参数以满足基于基本上一次暴露的评估的统计质量的预定要求。

公开(公告)号：US20060256340A1

公开(公告)日：2006-11-16

申请号：US11414839

申请日：2006-05-01

Applicant: Frans Hansen ,  Martin Glensbjerg ,  Borkur Arnvidarson ,  Jesper Jeppesen

Inventor： Frans Hansen ,  Martin Glensbjerg ,  Borkur Arnvidarson ,  Jesper Jeppesen

IPC: G01N21/00

CPC classification number: G01N15/1459 ,  B01L3/5027 ,  G01N15/1463 ,  G01N21/31 ,  G01N21/59 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/76 ,  G01N33/04 ,  G01N2015/025 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2021/5957 ,  G01N2021/6491

Abstract: The present invention relates to a method for the assessment of quantity and quality parameters of biological particles in a liquid analyte material. The method comprises applying a volume of a liquid sample to an exposing domain from which exposing domain electromagnetic signals from the sample in the domain can pass to the exterior, and exposing, onto an array of active detection elements such as CCD-elements, a spatial representation of electromagnetic signals having passed from the domain, the representation being detectable as an intensity by individual active detection elements, under conditions permitting processing of the intensities detected by the array of detection elements during the exposure in such a manner that representations of electromagnetic signals from the biological particles are identified as distinct from representations of electromagnetic signals from background signals. The size of the volume of the liquid sample is sufficiently large to permit the assessment of the quantity and quality parameters to fulfill a predetermined requirement to the statistical quality of the assessment based on substantially one exposure.

公开(公告)号：US20040246483A1

公开(公告)日：2004-12-09

申请号：US10727832

申请日：2003-12-04

Applicant: CHEMOMETEC A/S

Inventor： Frans Ejner Rvan Hansen ,  Martin Glensbjerg ,  Borkur Arnvidarson ,  Jesper Myron Jeppesen

IPC: G01N021/00

CPC classification number: G01N15/1459 ,  B01L3/5027 ,  G01N15/1463 ,  G01N21/31 ,  G01N21/59 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/76 ,  G01N33/04 ,  G01N2015/025 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2021/5957 ,  G01N2021/6491

Abstract: This invention relates to apparatuses for non-contact three-dimensional measurement of bodies and methods for determining a system of coordinates for measuring points on an apparatus for non-contact three-dimensional measurement of bodies. The apparatus and the method are characterized by particular simplicity and easy implementation. Advantageously, this makes the apparatus and method applicable in production sites for special workpieces. This opens up a wide and highly cost-efficient range of uses. Before the workpieces are measured, a system of coordinates for three-dimensional matching of the workpiece geometry is determined in a first measurement. A body with known dimensions of its edges or lines is placed on any position on the turntable and measured during one rotation using the triangulation sensor. The apparatus according to the invention is thus characterized by its minimal design. The low number of movements required, i.e. one translatory movement of the triangulation sensor and a rotational movement of the turntable, results in determining the outline of a body with a minimal error of measurement.

Abstract translation: 本发明涉及用于身体的非接触三维测量的装置和用于确定用于身体的非接触式三维测量的装置上的测量点的坐标系的方法。 该装置和方法的特征在于特别简单和容易实现。 有利地，这使得该装置和方法适用于特殊工件的生产现场。 这开辟了广泛和高性价比的使用范围。 在测量工件之前，在第一测量中确定工件几何形状的三维匹配的坐标系。 具有其边缘或线的已知尺寸的主体被放置在转台上的任何位置上，并且在使用三角测量传感器的一次旋转期间被测量。 因此，根据本发明的装置的特征在于其最小的设计。 所需的运动次数较少，即三角测量传感器的一个平移运动和转台的旋转运动导致以最小的测量误差来确定身体轮廓。

公开(公告)号：US20210156782A1

公开(公告)日：2021-05-27

申请号：US17048423

申请日：2019-04-17

Applicant: ChemoMetec A/S

Inventor： Johan Holm

IPC: G01N15/02 ,  G01N15/14 ,  G02B21/14

Abstract: A method for characterizing object(s) in a sample includes collecting transmitted, refracted, scattered, diffracted, and/or emitted light from the sample. The collected light formed on a sensor surface includes at least a first and second image of the object(s). For at least the first and second image, the collected light is modulated asymmetrically differently, or for at least a third and fourth image focal plane positions are different, or for at least a fifth image the collected light is modulated in at least two places differently compared to the surroundings, or for at least a sixth and seventh image of the object(s), the collected light is modulated asymmetrically differently and focal planes of the sixth and seventh image have different positions. The images are processed to characterize the objects(s).

公开(公告)号：US09102977B2

公开(公告)日：2015-08-11

申请号：US13577955

申请日：2011-02-10

Applicant: Søren Kjaerulff ,  Martin Glensbjerg

Inventor： Søren Kjaerulff ,  Martin Glensbjerg

IPC: C12Q1/68 ,  C12M1/34 ,  G01N21/64 ,  G01N21/66 ,  G01N31/22 ,  G01N33/52

CPC classification number: C12Q1/6806 ,  C12Q1/6895 ,  G01N21/6428 ,  G01N2021/6439 ,  C12Q2545/114 ,  C12Q2527/125 ,  C12Q2527/119

Abstract: Disclosed is a fast and simple method for quantification of nucleic acid of biological cells as 2-step protocol. In the first step cells are treated with an acidic solution containing a non-ionic detergent and a fluorescent DNA specific label. In the second step the sample may be neutralized. Determining of the content of nucleic can be performed by fluorescence microscopy. The method may also be used for obtaining information of cell cycle analysis, ploidy determination, measurements of nucleotide incorporation and assays for proliferation, health, stress level, apoptosis, necrosis, or other state of conditions of cells. The invention also relates to a kit of parts comprising an acidic agent, a detergent, a labelling agent and optionally a neutralization agent.

Abstract translation: 公开了一种用于定量生物细胞核酸的快速和简单的方法，作为2步方案。 在第一步中，用含有非离子洗涤剂和荧光DNA特异性标记的酸性溶液处理细胞。 在第二步中，样品可能被中和。 核酸含量的测定可以通过荧光显微镜进行。 该方法还可用于获得细胞周期分析，倍性测定，核苷酸掺入测定和增殖测定，健康，应激水平，细胞凋亡，坏死或细胞条件的其他状态的信息。 本发明还涉及包含酸性试剂，洗涤剂，标记试剂和任选的中和剂的试剂盒。

公开(公告)号：US20130129181A1

公开(公告)日：2013-05-23

申请号：US13699042

申请日：2011-05-18

Applicant: Martin Glensbjerg ,  Johan Christer Holm ,  Frans Ejner Ravn ,  Søren Kjaerulff

Inventor： Martin Glensbjerg ,  Johan Christer Holm ,  Frans Ejner Ravn ,  Søren Kjaerulff

IPC: G06K9/00

CPC classification number: G06K9/00147 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2015/1486 ,  G01N2015/1497 ,  G02B21/10 ,  G02B21/125 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: The invention relates to image analysis of dark field images obtained at low magnification below 10:1. Image analysis of dark field images obtained at low magnification can be combined with analyses of images obtained in respect of the same section of a sample and same magnification but with other techniques such as fluorescent microscopy. The system and method can be used e.g. for particle counting, particle size measurement, particle size distribution, morphology measurement, where the particles can be cells and/or cell parts. The invention also relates to a compact dark field light source unit, a system or apparatus including a microscope which by itself is compact and comprises the mentioned dark field light source unit.

Abstract translation: 本发明涉及以低于10:1的低倍率获得的暗场图像的图像分析。 在低倍率下获得的暗场图像的图像分析可以与对样品相同部分获得的图像和相同放大倍率的分析进行组合，但与其他技术如荧光显微镜组合。 该系统和方法可以例如使用。 用于颗粒计数，粒度测量，粒度分布，形态学测量，其中颗粒可以是细胞和/或细胞部分。 本发明还涉及一种紧凑的暗场光源单元，包括本身紧凑的包括所述暗场光源单元的显微镜的系统或设备。

公开(公告)号：US20110312013A1

公开(公告)日：2011-12-22

申请号：US13003982

申请日：2009-07-14

Applicant: Mette Elena Skindersø ,  Helle Frobøse Sørensen ,  Søren Kjærulff

Inventor： Mette Elena Skindersø ,  Helle Frobøse Sørensen ,  Søren Kjærulff

IPC: C12Q1/02 ,  C07D311/18

CPC classification number: G01N1/30 ,  C07K1/13 ,  G01N33/5005 ,  G01N33/502

Abstract: The present invention provides simple, rapid methods and procedures for analyzing cells, hereunder quantitative and qualitative assessment of cells. The present invention relates to the use of N-(9-acridinyl)maleimide (NAM) or to the use of 7-diethylamino-3-((4′-(iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI), particularly detectable upon its reaction with species (e.g., sulphur-containing species) present in higher concentrations in intact (e.g., living) cells than in non-intact (e.g., dead) cells. The present invention also relates to the use of NAM or to the use of 7-diethylamino-3-((4′-(iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI), particularly detectable upon its reaction with species present in intact and/or non-intact cells. Moreover, the present invention relates to the use of measuring techniques and/or instruments coupled with the use of NAM or with the use of 7-diethylamino-3-((4′-(iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI). The invention further relates to compositions used in methods for analyzing cells.

Abstract translation: 本发明提供用于分析细胞的简单，快速的方法和程序，以下对细胞进行定量和定性评估。 本发明涉及N-（9-吖啶基）马来酰亚胺（NAM）或7-二乙基氨基-3 - （（4' - （碘乙酰基）氨基）苯基）-4-甲基香豆素（CPI）的用途， 特别是与其在完整（例如活体）细胞中比在非完整（例如死细胞）中更高浓度存在的物质（例如，含硫物质）反应时可检测到。 本发明还涉及使用NAM或7-二乙基氨基-3 - （（4' - （碘乙酰基）氨基）苯基）-4-甲基香豆素（CPI）的用途，其特别可检测的与其存在于 完整和/或不完整的细胞。 此外，本发明涉及测量技术和/或仪器与使用NAM或使用7-二乙基氨基-3 - （（4' - （碘代乙酰基）氨基）苯基）-4-甲基香豆素（ CPI）。 本发明还涉及用于分析细胞的方法中使用的组合物。