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公开(公告)号：US11952401B2
公开(公告)日：2024-04-09
申请号：US17375203
申请日：2021-07-14
发明人： Haixue Zheng , Fan Yang , Zixiang Zhu , Xiangle Zhang , Weijun Cao , Congcong Wang , Kangli Li , Huisheng Liu , Hong Tian , Keshan Zhang , Xiangtao Liu
IPC分类号： C07K14/005 , A61K39/00 , A61K39/135 , A61P31/14 , A61P37/06 , C12N7/00
CPC分类号： C07K14/005 , A61K39/135 , A61P31/14 , A61P37/06 , C12N7/00 , A61K2039/525 , A61K2039/552 , C12N2770/32121 , C12N2770/32122 , C12N2770/32134 , C12N2770/32152 , C12N2770/32171
摘要： The present disclosure belongs to the technical field of biological products for veterinary medicine, and specifically relates to a recombinant foot-and-mouth disease virus (FMDV) with a reduced immunosuppressive activity, a preparation method and use thereof, and a recombinant vaccine strain. According to the present disclosure, it is firstly discovered that FMDV 3B protein has an immunosuppressive function, and key sites for exerting the immunosuppressive function are found. A recombinant FMDV vaccine strain with a lost immunosuppressive function in FMDV 3B protein is constructed by introducing amino acid mutations into three repeated copies of FMDV 3B protein.
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公开(公告)号：US11883482B2
公开(公告)日：2024-01-30
申请号：US17328135
申请日：2021-05-24
发明人： Haixue Zheng , Fan Yang , Zixiang Zhu , Weijun Cao , Hong Tian , Keshan Zhang , Ting Wei , Min Zheng , Wei Zhang , Wen Dang , Xusheng Ma , Dan Li , Yi Ru , Jijun He , Jianhong Guo , Xiangtao Liu
IPC分类号： C12N7/00 , C12N15/66 , C12N15/10 , A61K39/125 , A61K39/00
CPC分类号： A61K39/125 , C12N15/102 , C12N15/66 , A61K2039/5252 , A61K2039/53 , C12N7/00 , C12N2770/32034
摘要： The disclosure provides a recombinant nucleic acid of Seneca valley virus, a recombinant vaccine strain and preparation method and use thereof, and relates to the technical field of genetic engineering. The disclosure provides the recombinant nucleic acid of Seneca valley virus, recombinant Seneca valley virus comprising the recombinant nucleic acid, recombinant Seneca valley virus encoded by the recombinant nucleic acid, recombinant Seneca valley virus vaccine strain comprising the recombinant Seneca valley virus and preparation method and use thereof. According to the disclosure, a vaccine strain characterized by high antigen production capacity, remarkably reduced pathogenicity (even having no pathogenicity to pigs), strong antibody induction activity, high immune protection rate is prepared. The vaccine strain remarkably improves the biological safety and can be used for preventing and controlling Seneca valley virus in China and the neighboring countries.
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公开(公告)号：US11866703B2
公开(公告)日：2024-01-09
申请号：US17133300
申请日：2020-12-23
发明人： Jianping Cai , Zigang Qu , Xiao Xu , Jing Wang , Zhenxing Gong , Heng Wang , Xueyang He , Wenqing Wang
IPC分类号： C12N15/113 , C12N9/22 , C12N15/79 , C12N15/10
CPC分类号： C12N15/113 , C12N9/22 , C12N15/1031 , C12N15/79 , C12N2310/20
摘要： The present disclosure provides a method for knocking out an N-myristoyltransferase (NMT) gene from Eimeria tenella, and belongs to the technical field of microorganisms. The method includes: mixing sporozoites of Eimeria tenella with a pCRISPR::EtNMT plasmid and a pEtNMT::DHFR plasmid, and subjecting a resulting mixture to electrotransformation to obtain NMT gene-knockout Eimeria tenella. The method provided by the present disclosure can successfully knock out the NMT gene from Eimeria tenella, which lays a foundation for studying the function of the Eimeria tenella gene and developing a vaccine therefor.
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4.发明授权公开(公告)号：US11801289B2
公开(公告)日：2023-10-31
申请号：US17335573
申请日：2021-06-01
发明人： Yuefeng Chu , Shengli Chen , Huafang Hao , Xinmin Yan , Lina Ma , Yongsheng Liu
CPC分类号： A61K39/0241 , A61K39/39 , A61K2039/55566 , A61K2039/55577 , A61K2039/55588
摘要： The present disclosure provides a subunit vaccine of contagious caprine pleuropneumonia and preparation method and use thereof, belonging to the technical field of preparation of animal infectious disease vaccine. The combination of Mycoplasma capricolum subsp. capripneumoniae immunoproteins comprises Mycoplasma capricolum subsp. capripneumoniae immunoproteins A, B, C, D and E; the mass ratio of Mycoplasma capricolum subsp. capripneumoniae immunoproteins A, B, C, D and E is (0.5-1.5):(0.5-1.5):(0.5-1.5):(0.5-1.5):(0.5-1.5). The subunit vaccine of contagious caprine pleuropneumonia comprises the combination of Mycoplasma capricolum subsp. capripneumoniae immunoproteins and adjuvant; the subunit vaccine has the advantages of high safety, good immunization effect, high stability and minor adverse effects.
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公开(公告)号：US20210016272A1
公开(公告)日：2021-01-21
申请号：US17037826
申请日：2020-09-30
发明人： Shiqi SUN , Huichen GUO , Yun ZHANG , Yanyan CHANG , Yanquan WEI , Jiaxi RU , Xiaoying ZHI , Ping DU , Xiangtao LIU , Hong YIN , Jianxun LUO
IPC分类号： B01L3/00 , G01N33/569
摘要： A test strip for detecting a serotype O foot-and-mouth disease, the test strip including: a bottom board; a detection layer being disposed on the bottom board and including a detection line and a control line; an absorbent layer being disposed at one end of the detection layer close to the control line; a gold colloidal conjugate pad being disposed at the other side of the detection layer close to the detection line; and a sample pad is disposed on a top of the gold colloidal conjugate pad. The gold colloidal conjugate pad is coated with colloidal gold particles that are conjugated with a Staphylococcus protein A (SPA) marker. The detection line is coated or impregnated with serotype O FMDV-like particles, and the control line is coated or impregnated with rabbit IgG.
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公开(公告)号：US20240148850A1
公开(公告)日：2024-05-09
申请号：US17997749
申请日：2022-03-17
发明人： Jing ZHANG , Zengjun LU , Kun LI , Pu SUN , Jian WANG , Yimei CAO , Huifang BAO , Zhixun ZHAO , Pinghua LI , Yuanfang FU , Xueqing MA , Hong YUAN , Xingwen BAI , Qiang ZHANG , Dong LI , Zaixin LIU
IPC分类号： A61K39/12 , A61K39/00 , C07K14/005 , G01N33/569
CPC分类号： A61K39/12 , C07K14/005 , G01N33/56983 , A61K2039/5252 , A61K2039/5254 , C12N2770/10022 , C12N2770/10034 , G01N2333/08
摘要： The present disclosure provides an N protein epitope mutation marker for preparing an epitope deletion-marked vaccine strain of type II porcine reproductive and respiratory syndrome virus (PRRSV) and use thereof, belonging to the technical field of biological products. In the mutation marker, one or more amino acids are mutated based on an epitope sequence at positions 92 to 103 of a C-terminal of an N protein of the type II PRRSV; and the epitope mutation marker has an amino acid sequence shown in SEQ ID NO: 1, where X1 is selected from the group consisting of T, P, and A; and X2 is selected from the group consisting of V and A.
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公开(公告)号：US11883411B2
公开(公告)日：2024-01-30
申请号：US17895390
申请日：2022-08-25
发明人： Keshan Zhang , Haixue Zheng , Huimei Cui , Bo Yang , Hong Tian , Zixiang Zhu , Tao Feng , Fan Yang , Weijun Cao , Xusheng Ma , Yi Ru , Jianhong Guo , Xiangtao Liu
IPC分类号： A61K31/5377 , A61P31/20
CPC分类号： A61K31/5377 , A61P31/20
摘要： The present disclosure belongs to the technical field of African swine fever (ASF) treatment, in particular to use of a compound aprepitant in prevention or treatment of the ASF. In the present disclosure, it is found that the compound aprepitant can significantly inhibit replication of an African swine fever virus (ASFV); in addition, it is found that the aprepitant inhibits transcription and protein expression of D1133L, and reduces transcription and protein expression levels of p30 and p72, preventing virus invasion on host cells. Therefore, the compound aprepitant can be used to inhibit early and late infection of the ASFV. Accordingly, the compound aprepitant can be used to prevent or treat the ASF.
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8.发明公开公开(公告)号：US20230270747A1
公开(公告)日：2023-08-31
申请号：US17895390
申请日：2022-08-25
发明人： Keshan ZHANG , Haixue ZHENG , Huimei CUI , Bo YANG , Hong TIAN , Zixiang ZHU , Tao FENG , Fan YANG , Weijun CAO , Xusheng MA , Yi RU , Jianhong GUO , Xiangtao LIU
IPC分类号： A61K31/5377 , A61P31/20
CPC分类号： A61K31/5377 , A61P31/20
摘要： The present disclosure belongs to the technical field of African swine fever (ASF) treatment, in particular to use of a compound aprepitant in prevention or treatment of the ASF. In the present disclosure, it is found that the compound aprepitant can significantly inhibit replication of an African swine fever virus (ASFV); in addition, it is found that the aprepitant inhibits transcription and protein expression of D1133L, and reduces transcription and protein expression levels of p30 and p72, preventing virus invasion on host cells. Therefore, the compound aprepitant can be used to inhibit early and late infection of the ASFV. Accordingly, the compound aprepitant can be used to prevent or treat the ASF.
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9.发明公开公开(公告)号：US20230218736A1
公开(公告)日：2023-07-13
申请号：US17948752
申请日：2022-09-20
发明人： Junjun SHAO , Guanglei ZHANG , Wei LIU , Xiaxia LIANG , Huiyun CHANG
IPC分类号： A61K39/12 , C07K14/005 , A61K39/295 , A61P31/20
CPC分类号： A61K39/12 , C07K14/005 , A61K39/295 , A61P31/20 , C12N2710/12034 , C12N2710/12022 , A61K2039/552
摘要： The present disclosure relates to a cocktail vaccine of a recombinant African swine fever virus (ASFV) antigen and use thereof, and belongs to the field of biotechnology pharmacy. The cocktail vaccine is prepared by combination of recombinant proteins p30-modified p54, p72 epitope-N-segment amino acid sequence of pE248R, and N-terminal amino acid sequence of CD2v-C-terminal amino acid sequence of pEP153R with an adjuvant. The present disclosure provides a method for expressing and purifying the three recombinant proteins, as well as a ratio of each recombinant protein and the adjuvant for preparing the cocktail vaccine.
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公开(公告)号：US10532077B2
公开(公告)日：2020-01-14
申请号：US16413598
申请日：2019-05-15
发明人： Huichen Guo , Shiqi Sun , Shichong Han , Hu Dong , Xiaoran Guo , Hong Yin , Jianxun Luo
IPC分类号： C07K14/005 , C07K16/10 , C12N7/00 , A61K39/00 , A61K39/12 , A61K35/76 , C12N15/86 , A61P31/14 , A61K39/39
摘要： A virus-like particle of Senecavirus A, the particle including a structural protein VP0, a structural protein VP1 and a structural protein VP3. The structural protein VP0 is encoded by a gene sequence represented by SEQ ID NO: 1. The structural protein VP1 is encoded by a gene sequence represented by SEQ ID NO: 2. The structural protein VP3 is encoded by a gene sequence represented by SEQ ID NO: 3.
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