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    CATIONIC TAGS FOR ATTOMOLE LEVEL DETECTION OF ANALYTES BY MASS SPECTROMETRY
    2.
    发明申请
    CATIONIC TAGS FOR ATTOMOLE LEVEL DETECTION OF ANALYTES BY MASS SPECTROMETRY 审中-公开
    通过质谱分析仪检测分析物的角度标签

    公开(公告)号：WO2016123633A1

    公开(公告)日：2016-08-04

    申请号：PCT/US2016/016022

    申请日：2016-02-01

    申请人： NORTHEASTERN UNIVERSITY

    发明人： GIESE, Roger ,  WANG, Poguang

    IPC分类号： G01N33/50

    CPC分类号： G01N33/52 ,  C07C217/48 ,  C07C217/58 ,  G01N2458/15 ,  G01N2560/00

    摘要： The invention provides methods of detecting an analyte by multi-stage mass spectrometry with improved S/N ratio. An analyte is labeled with a positively- charged mass tag to form a precursor ion that leads by anchimeric assistance to a greatly enhanced, analyte-characteristic first product ion that can, in turn, lead to a greatly enhanced, analyte-characteristic second product ion in a mass spectrometer. Either a three stage mass spectrometer (true MS3) or a two-stage mass spectrometer (MS2) operated in a pseudo MS3 mode can be used. The precursor ion is split via an anchimeric-assisted reaction to form a first product ion, which in turn can be fragmented to form the second product ion. The methods offer extreme ultrasensitivity, at the low amol level. The invention also provides anchimeric mass tags for use in the methods. A wide variety of previously undetectable analytes of biological or environmental origin can be detected and quantified.

    摘要翻译： 本发明提供了通过具有改善的S / N比的多级质谱检测分析物的方法。 分析物用带正电荷的质量标签标记以形成前体离子，其通过主动协助引导到大大增强的分析物特征的第一产物离子，从而导致大大增强的分析物特征的第二产物离子 在质谱仪中。 可以使用以伪MS3模式操作的三级质谱仪（真MS3）或两级质谱仪（MS2）。 前体离子通过锚定辅助反应分离以形成第一产物离子，其又可以被分段以形成第二产物离子。 该方法在低amol水平提供极端的超敏感性。 本发明还提供了用于该方法的锚定质粒标签。 可以检测和量化生物或环境来源的各种先前不可检测的分析物。