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    Fixation of nucleotide derivatives to solid carrier
    2.
    发明授权
    Fixation of nucleotide derivatives to solid carrier 有权
    核苷酸衍生物固定于固体载体

    公开(公告)号：US06664051B1

    公开(公告)日：2003-12-16

    申请号：US09706405

    申请日：2000-11-03

    申请人： Hiroshi Shinoki ,  Yukio Sudo ,  Osamu Seshimoto

    发明人： Hiroshi Shinoki ,  Yukio Sudo ,  Osamu Seshimoto

    IPC分类号： C07H2104

    CPC分类号： C40B40/06 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00677 ,  B82Y30/00 ,  C40B50/06

    摘要： A DNA chip or its analogue composed of nucleotide derivatives attached to a solid carrier is prepared by method of bringing nucleotide derivatives having a reactive group at each one terminal into contact with a solid carrier having thereon reactive groups in an aqueous phase in the presence of a transferase which is capable of producing a covalent bond by rearrangement of the reactive group of the nucleotide derivative and the reactive group of the solid carrier.

    摘要翻译： 通过以下方法制备DNA芯片或其由连接于固体载体上的核苷酸衍生物组成的类似物的方法：使得在每个末端具有反应性基团的核苷酸衍生物与其中具有反应性基团的固体载体在水相中存在下接触 能够通过核苷酸衍生物的反应性基团和固体载体的反应性基团的重排而产生共价键的转移酶。

    Fluorescent substances
    4.
    发明授权
    Fluorescent substances 失效
    荧光物质

    公开(公告)号：US06642375B2

    公开(公告)日：2003-11-04

    申请号：US09731279

    申请日：2000-12-06

    申请人： Hiroko Inomata ,  Hiroshi Shinoki ,  Masayoshi Kojima ,  Yukio Sudo ,  Junji Nishigaki ,  Osamu Seshimoto

    发明人： Hiroko Inomata ,  Hiroshi Shinoki ,  Masayoshi Kojima ,  Yukio Sudo ,  Junji Nishigaki ,  Osamu Seshimoto

    IPC分类号： C07H1904

    CPC分类号： G01N33/582

    摘要： The present invention provides a fluorescent substance which is represented by a formula: A-B-C wherein A is a residue of natural or synthetic nucleotide, oligonucleotide, polynucleotide, or derivative thereof, and binds to B at a base moiety in said residue, or A is a residue of avidin or streptavidin; B is a divalent linking group or a single bond; and C is a monovalent group derived from a general formula (I) and binds to B at a reactive group present in R1 or R2: wherein R1 and R2 each independently represent an alkyl group that may be substituted with a reactive group capable of covalently bonding to A-B-; R3, R4, R5, and R6 each independently represent an alkyl group, and R3 and R4, and/or R5 and R6 may bind to each other to form a saturated carbon-ring together with a carbon atom(s) to which they bind; V1, V2, V3, V4, V5, V6, V7, V8, V9 and V10 each independently represent a hydrogen atom or a monovalent substituent, and two adjacent groups thereof may bind to form a ring; L1, L2, and L3 represent a substituted or unsubstituted methine group; each of m, n, s, and t represents 0 or 1, provided that m+n=1 and s+t=1; p represents 1, 2, or 3; M represents a counter ion; and q represents a number required to neutralize the charge of a molecule. The fluorescent substance of the present invention is useful as a labeling substance for nucleic acids, or as a reagent for analyzing biological components such as nucleic acids, proteins or sugars.

    摘要翻译： 本发明提供一种荧光物质，其由下式表示：AB-C，其中A是天然或合成的核苷酸，寡核苷酸，多核苷酸或其衍生物的残基，并与所述残基中的碱基部分的B结合，或A是 抗生物素蛋白或链霉亲和素的残基; B是二价连接基团或单键; 和C是衍生自通式（I）的一价基团，并且在R 1或R 2中存在的反应性基团上与B结合：其中R 1和R 2各自独立地表示烷基， 可以被能够与AB-共价结合的反应性基团取代; R 3，R 4，R 5和R 6各自独立地表示烷基，R 3和R 4和/或R 5和R 6独立地表示烷基， 可以彼此结合形成饱和碳环与它们所结合的碳原子一起; V 1，V 2，V 3，V 4，V 5，V 6，V 7，V 8，V 9和V 10 独立地表示氢原子或一价取代基，其两个相邻基团可以结合形成环; L 1，L 2和L 3表示取代或未取代的次甲基; m，n，s和t中的每一个表示0或1，条件是m + n = 1且s + t = 1; p表示1,2或3; M表示抗衡离子; q表示中和分子电荷所需的数。 本发明的荧光物质可用作核酸的标记物质，或用作分析核酸，蛋白质或糖类等生物成分的试剂。

    Homogenous enzyme immunoassay process
    5.
    发明授权
    Homogenous enzyme immunoassay process 失效
    均质酶免疫测定法

    公开(公告)号：US06287785B1

    公开(公告)日：2001-09-11

    申请号：US09488371

    申请日：2000-01-20

    申请人： Hiroshi Shinoki ,  Osamu Seshimoto

    发明人： Hiroshi Shinoki ,  Osamu Seshimoto

    IPC分类号： G01N33533

    CPC分类号： G01N33/535 ,  G01N33/542 ,  G01N33/54306 ,  Y10S435/963 ,  Y10S435/964 ,  Y10S435/969 ,  Y10S435/972 ,  Y10S436/823 ,  Y10S530/866

    摘要： An improved homogeneous enzyme immunoassay process for quantitatively analyzing an antigen by determining the change in the enzymatic activity caused by a reaction between the antigen and an enzyme-labeled antibody. The antigen is reacted with an enzyme-labeled antibody, followed by the reaction with a second antibody capable of recognizing and binding to a different epitope and then with a third antibody capable of recognizing and binding to the second antibody. The enzymatic activity of the labeling enzyme is determined by a water-insoluble substrate. Using the water-insoluble substrate, steric hindrance is enhanced. A highly-sensitive analysis can be carried out by a simple operation even when the antigen has a molecular weight falling within an intermediate range, for example, a range of M.W. 10,000 to 70,000.

    摘要翻译： 一种改进的均匀酶免疫测定方法，用于通过测定由抗原和酶标记的抗体之间的反应引起的酶活性的变化来定量分析抗原。 使抗原与酶标记的抗体反应，然后与能够识别和结合不同表位的第二抗体反应，然后与能够识别和结合第二抗体的第三抗体反应。 标记酶的酶活性由水不溶性底物决定。 使用水不溶性底物，空间位阻增强。 即使当抗原具有分子量落在中间范围内时，也可以通过简单的操作进行高灵敏度分析，例如，10,000至70000MW的范围。

    Measurement chip for surface plasmon resonance biosensor
    9.
    发明授权
    Measurement chip for surface plasmon resonance biosensor 有权
    表面等离子体共振生物传感器测量芯片

    公开(公告)号：US06726881B2

    公开(公告)日：2004-04-27

    申请号：US10230340

    申请日：2002-08-29

    申请人： Hiroshi Shinoki ,  Osamu Seshimoto

    发明人： Hiroshi Shinoki ,  Osamu Seshimoto

    IPC分类号： G01N2175

    CPC分类号： G01N21/553 ,  G01N33/54393 ,  G01N33/553

    摘要： The present invention provides a measurement chip for a surface plasmon resonance biosensor, which comprises a transparent substrate, a metal membrane located on the transparent substrate and an organic silicon membrane immobilized on the metal membrane and in which the organic silicon membrane is immobilized on the metal membrane via a functional group capable of binding with atoms on the surface of a metal. The measurement chip for a biosensor of the present invention can easily be produced. Using the measurement chip of the present invention, a target substance can be measured with good sensitivity, even if only a small amount of physiologically active substance is immobilized.

    摘要翻译： 本发明提供了一种用于表面等离子体共振生物传感器的测量芯片，其包括透明基板，位于透明基板上的金属膜和固定在金属膜上的有机硅膜，其中有机硅膜固定在金属上 膜通过能够与金属表面上的原子结合的官能团。 本发明的生物传感器用测量芯片可以容易地制造。 使用本发明的测量芯片，即使只固定少量的生理活性物质，也能够以良好的灵敏度测定目标物质。