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    Chemical ligation dependent probe amplification (CLPA)
    5.
    发明授权
    Chemical ligation dependent probe amplification (CLPA) 有权

    公开(公告)号：US09976177B2

    公开(公告)日：2018-05-22

    申请号：US12798108

    申请日：2010-03-29

    申请人： Robert Terbrueggen

    发明人： Robert Terbrueggen

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/6855 ,  C12Q1/6862 ,  C12Q2523/109 ,  C12Q2525/15 ,  C12Q2525/155 ,  C12Q2525/204

    摘要： The present invention provides compositions, apparatuses and methods for detecting one or more nucleic acid targets present in a sample. Methods of the invention include utilizing two or more oligonucleotide probes that reversibly bind a target nucleic acid in close proximity to each other and possess complementary reactive ligation moieties. When such probes have bound to the target in the proper orientation, they are able to undergo a spontaneous chemical ligation reaction that yields a ligated oligonucleotide product. In one aspect, the ligation product is of variable length that correlates with a particular target. Following chemical ligation, the probes may be amplified and detected by capillary electrophoresis or microarray analysis.

    Detection of nucleic acid targets using chemically reactive oligonucleotide probes
    7.
    发明申请
    Detection of nucleic acid targets using chemically reactive oligonucleotide probes 审中-公开
    使用化学反应寡核苷酸探针检测核酸靶

    公开(公告)号：US20080124810A1

    公开(公告)日：2008-05-29

    申请号：US11801990

    申请日：2007-05-10

    申请人： Robert Terbrueggen

    发明人： Robert Terbrueggen

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6827 ,  C12Q1/6818 ,  C12Q1/6858 ,  Y10T436/143333 ,  C12Q2523/109 ,  C12Q2533/107 ,  C12Q2565/514

    摘要： The present invention provides compositions, apparatuses and methods for detecting one or more nucleic acid targets present in a sample. Methods of the invention include utilizing two or more oligonucleotide probes that reversibly bind a target nucleic acid in close proximity to each other and possess complementary reactive ligation moieties. When such probes have bound to the target in the proper orientation, they are able to undergo a spontaneous chemical ligation reaction that yields a ligated oligonucleotide product. In accordance with the invention, the presence of the target(s) of interest can be determined by measuring the presence or amount of ligated oligonucleotide product.

    摘要翻译： 本发明提供了用于检测样品中存在的一种或多种核酸靶的组合物，装置和方法。 本发明的方法包括利用两个或更多个寡核苷酸探针，其可逆地结合彼此紧邻的靶核酸并具有互补的反应性连接部分。 当这种探针以正确的方向结合靶时，它们能够进行自发化学连接反应，产生连接的寡核苷酸产物。 根据本发明，可以通过测量连接的寡核苷酸产物的存在或量来确定感兴趣的靶标的存在。

    Detection of nucleic acid targets using chemically reactive oligonucleotide probes
    8.
    发明授权
    Detection of nucleic acid targets using chemically reactive oligonucleotide probes 有权

    公开(公告)号：US10829803B2

    公开(公告)日：2020-11-10

    申请号：US11801990

    申请日：2007-05-10

    申请人： Robert Terbrueggen

    发明人： Robert Terbrueggen

    IPC分类号： C12Q1/68 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/6818 ,  C07H21/04

    摘要： The present invention provides compositions, apparatuses and methods for detecting one or more nucleic acid targets present in a sample. Methods of the invention include utilizing two or more oligonucleotide probes that reversibly bind a target nucleic acid in close proximity to each other and possess complementary reactive ligation moieties. When such probes have bound to the target in the proper orientation, they are able to undergo a spontaneous chemical ligation reaction that yields a ligated oligonucleotide product. In accordance with the invention, the presence of the target(s) of interest can be determined by measuring the presence or amount of ligated oligonucleotide product.