公开(公告)号：US20050084421A1

公开(公告)日：2005-04-21

申请号：US10837885

申请日：2004-05-02

Applicant: Marc Unger ,  Jiang Huang ,  Emerson Quan

Inventor： Marc Unger ,  Jiang Huang ,  Emerson Quan

IPC: B01L3/00

CPC classification number: B01L3/00 ,  B01L3/0248 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0638 ,  C12Q1/686

Abstract: An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.

Abstract translation: 一种用于执行反应矩阵的MxN矩阵微流体装置，所述装置具有多个反应池，其通过形成在所述装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。

公开(公告)号：US20050065735A1

公开(公告)日：2005-03-24

申请号：US10976643

申请日：2004-10-29

Applicant: Michael Lee ,  Gajus Worthington ,  Gregory Harris ,  James Montgomery

Inventor： Michael Lee ,  Gajus Worthington ,  Gregory Harris ,  James Montgomery

IPC: B01F13/00 ,  B01F15/00 ,  B01L3/00 ,  B81B1/00 ,  B81C99/00 ,  G06F17/50 ,  G06G7/48 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00 ,  G06G7/58

CPC classification number: G06F17/50 ,  B01F13/0059 ,  B01F15/00824 ,  B01F15/00935 ,  B01J2219/00997 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L2200/12 ,  B81B1/00 ,  B81C99/006 ,  G06F17/509 ,  G06F2217/16 ,  H01H2029/008

Abstract: The present invention generally relates to microfluidics and more particularly to the design of customized microfluidic systems using a microfluidic computer aided design system. In one embodiment of the present invention a microfluidic circuit design method is provided. The method includes developing synthesizable computer code for a design. Next, a microfluidic circuit schematic, including a plurality of symbols for microfluidic components, is generated either interactively or using the synthesizable computer code. The microfluidic circuit schematic is then functionally simulated. The microfluidic components are placed and routed on a template to form a physical layout. Then the physical layout is physically simulated using dynamic simulation models of the microfluidic components; and the physical layout is written to a layout file.

Abstract translation: 本发明一般涉及微流体，更具体地涉及使用微流控计算机辅助设计系统的定制微流体系统的设计。 在本发明的一个实施例中，提供了一种微流体电路设计方法。 该方法包括开发用于设计的可综合计算机代码。 接下来，包括用于微流体成分的多个符号的微流体电路原理图被交互地产生或使用可合成的计算机代码。 然后在功能上模拟微流体电路原理图。 将微流体组件放置并在模板上布线以形成物理布局。 然后使用微流体组件的动态模拟模型物理模拟物理布局; 并将物理布局写入布局文件。

公开(公告)号：US11857940B2

公开(公告)日：2024-01-02

申请号：US17398865

申请日：2021-08-10

Applicant: Fluidigm Corporation

Inventor： Peilin Chen

IPC: C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6844 ,  B01J19/00

CPC classification number: B01J19/0046 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  B01J2219/00585 ,  B01J2219/00722 ,  C12Q1/6848 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q1/6853 ,  C12Q2525/161 ,  C12Q2525/301

Abstract: The present disclosure provides a “looping amplification” method to increase the specificity of nucleic acid amplification. This increased specificity facilitates multiplexing to a much higher degree than was previously possible.

公开(公告)号：US20210324446A1

公开(公告)日：2021-10-21

申请号：US17166992

申请日：2021-02-03

Applicant: Fluidigm Corporation

Inventor： Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

IPC: C12Q1/6806 ,  C12Q1/686 ,  B01L3/00

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：US11117113B2

公开(公告)日：2021-09-14

申请号：US15382360

申请日：2016-12-16

Applicant: Fluidigm Corporation

Inventor： Peilin Chen

IPC: C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6844 ,  B01J19/00

Abstract: The present disclosure provides a “looping amplification” method to increase the specificity of nucleic acid amplification. This increased specificity facilitates multiplexing to a much higher degree than was previously possible.

公开(公告)号：US10344318B2

公开(公告)日：2019-07-09

申请号：US15590998

申请日：2017-05-09

Applicant: Fluidigm Corporation

Inventor： Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

IPC: C12Q1/686 ,  C12Q1/6806 ,  B01L3/00 ,  B01L7/00

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：US09952134B2

公开(公告)日：2018-04-24

申请号：US13294799

申请日：2011-11-11

Applicant: Dmitry R. Bandura ,  Vladimir I. Baranov ,  Scott D. Tanner

Inventor： Dmitry R. Bandura ,  Vladimir I. Baranov ,  Scott D. Tanner

IPC: H01J49/00 ,  G01N15/14 ,  H01J49/04 ,  G01N15/00

CPC classification number: G01N15/1404 ,  G01N2015/0065 ,  G01N2458/15 ,  H01J49/004 ,  H01J49/04 ,  H01J49/0431

Abstract: An analytical instrument for cellular analysis of cellular particles tagged with elemental tags, such as lanthanide-based elemental tags. The analytical instrument has a sample introduction system for generating a stream of particles from the sample. An inductively coupled plasma ionization system atomizes and ionizes particles in the stream as they are received. The instrument has an ion pretreatment system and a mass analyzer. The ion pretreatment system is adapted to transport ions generated by the ionization system to the mass analyzer. The ion pretreatment system can filter out low mass ions, such as using a high-pass mass filter or a bandpass mass filter, to allow the elemental tags to pass therethrough. The mass analyzer is adapted to measure the amount of at least one element in individual particles from the stream by performing mass analysis on the ions from the atomized particles. The instrument can be adapted to measure the amount of many different tags, for example at least five different tags, at the same time to facilitate multi-parametric analysis of cells and other particles.

公开(公告)号：US09909179B2

公开(公告)日：2018-03-06

申请号：US14977414

申请日：2015-12-21

Applicant: Fluidigm Corporation

Inventor： Amy Hamilton ,  Min Lin ,  Alain Mir ,  Martin Pieprzyk

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2531/119 ,  C12Q2531/137 ,  C12Q2565/101 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/178

Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.

公开(公告)号：US09868978B2

公开(公告)日：2018-01-16

申请号：US15013915

申请日：2016-02-02

Applicant: Fluidigm Corporation

Inventor： Stanley N. Lapidus

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2565/518 ,  C12Q2563/107 ,  C12Q2533/101

Abstract: The invention provides methods and devices for detecting, enumerating or identifying target nucleic acid molecules using immobilized capture probes and single molecule sequencing techniques.

公开(公告)号：US09815055B2

公开(公告)日：2017-11-14

申请号：US15242187

申请日：2016-08-19

Applicant: Fluidigm Corporation

Inventor： Jason A. A. West ,  Jesse Thompson

IPC: B01L3/00 ,  G01N33/00 ,  B29C45/03 ,  B29C59/02 ,  B01J19/00 ,  B29C45/00 ,  B29C45/73 ,  B05D5/00 ,  B32B37/06 ,  G01N33/543 ,  B29L31/00

CPC classification number: B01L3/502707 ,  B01J19/0046 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00722 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0887 ,  B01L2300/12 ,  B29L2031/756 ,  G01N33/54386

Abstract: Provided are microfluidic devices and methods for fabricating and bonding such devices. Also provided are kits for analyzing analyte-containing samples and for lysing cells.