公开(公告)号：US20150151307A1

公开(公告)日：2015-06-04

申请号：US14520510

申请日：2014-10-22

Applicant: Berkeley Lights, Inc.

Inventor： Keith J. Breinlinger ,  Daniele Malleo ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Alexander H. Slocum ,  Mark P. White

IPC: B03C7/02 ,  B81B7/02

CPC classification number: B81B7/02 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0433 ,  B01L2400/0454 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  B03C7/023 ,  B03C2201/26 ,  C12M23/16 ,  C12M41/46 ,  C12M47/02 ,  G01N27/447 ,  G01N27/44756 ,  G01N27/44791 ,  G01N33/5023 ,  G01N33/543 ,  G01N33/54326 ,  G01N35/10

Abstract: A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.

Abstract translation: 微流体装置中的保持笔中的一组微物体可以被选择并移动到分段区域，微物体可以从微流体装置输出。 微流体装置可以具有多个保持笔，并且每个保持笔可以将位于保持笔中的微物体与位于另一个保持笔中的微物体或微流体装置中的其他地方隔离。 所选择的一组微物体可以包含一个或多个生物细胞，例如克隆的细胞群。 因此，本发明的实施方案可以在微流体装置中选择特定的一组克隆细胞，将克隆细胞移动到分期区域，并从微流体装置输出克隆细胞，同时保持输出组的克隆性质。

公开(公告)号：US20200284756A1

公开(公告)日：2020-09-10

申请号：US16869399

申请日：2020-05-07

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Igor Y. Khandros ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Ming C. Wu

IPC: G01N27/447 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02

Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.

公开(公告)号：US10690628B2

公开(公告)日：2020-06-23

申请号：US15842124

申请日：2017-12-14

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Igor Y. Khandros ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Ming C. Wu

IPC: G01N27/447 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  G01N27/416 ,  G01N27/26

Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.

公开(公告)号：US10569271B2

公开(公告)日：2020-02-25

申请号：US15785727

申请日：2017-10-17

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  BERKELEY LIGHTS, INC.

Inventor： Ming-Chiang Wu ,  Jodi Tsu-An Loo ,  Shao Ning Pei ,  Gaetan L. Mathieu ,  Jian Gong ,  Randall D. Lowe, Jr. ,  Justin K. Valley

IPC: B01L99/00 ,  B01L3/00 ,  G01N27/447

Abstract: Single-sided optoelectrowetting (SSOEW)-configured substrates are provided, as well as microfluidic devices that include such substrates. The substrates can include a planar electrode, a photoconductive (or photosensitive) layer, a dielectric layer (single-layer or composite), a mesh electrode, and a hydrophobic coating. Fluid droplets can be moved across the hydrophobic coating of such substrates in a light-actuated manner, upon the application of a suitable AC voltage potential across the substrate and the focusing of light into the photoconductive layer of the substrate in a location proximal to the droplets. Walls can be disposed upon the substrates to form the microfluidic devices. Together the walls and substrate can form a microfluidic circuit, through which droplets can be moved.

公开(公告)号：US10252907B2

公开(公告)日：2019-04-09

申请号：US15439506

申请日：2017-02-22

Applicant: Berkeley Lights, Inc.

Inventor： Keith J. Breinlinger ,  Daniele Malleo ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Alexander H. Slocum ,  Mark P. White

IPC: B81B7/02 ,  B03C5/00 ,  B03C5/02 ,  G01N27/447 ,  G01N33/50 ,  B03C7/02 ,  G01N33/543 ,  B01L3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/34 ,  G01N35/10

Abstract: A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.

公开(公告)号：US20180099275A1

公开(公告)日：2018-04-12

申请号：US15785727

申请日：2017-10-17

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  BERKELEY LIGHTS, INC.

Inventor： Ming-Chiang Wu ,  Jodi Tsu-An Loo ,  Shao Ning Pei ,  Gaetan L. Mathieu ,  Jian Gong ,  Randall D. Lowe, JR. ,  Justin K. Valley

IPC: B01L3/00 ,  G01N27/447

Abstract: Single-sided optoelectrowetting (SSOEW)-configured substrates are provided, as well as microfluidic devices that include such substrates. The substrates can include a planar electrode, a photoconductive (or photosensitive) layer, a dielectric layer (single-layer or composite), a mesh electrode, and a hydrophobic coating. Fluid droplets can be moved across the hydrophobic coating of such substrates in a light-actuated manner, upon the application of a suitable AC voltage potential across the substrate and the focusing of light into the photoconductive layer of the substrate in a location proximal to the droplets. Walls can be disposed upon the substrates to form the microfluidic devices. Together the walls and substrate can form a microfluidic circuit, through which droplets can be moved.

公开(公告)号：US09815056B2

公开(公告)日：2017-11-14

申请号：US14960010

申请日：2015-12-04

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  BERKELEY LIGHTS, INC.

Inventor： Ming-Chiang Wu ,  Jodi Tsu-An Loo ,  Shao Ning Pei ,  Gaetan L. Mathieu ,  Jian Gong ,  Randall D. Lowe, Jr. ,  Justin K. Valley

IPC: B01L99/00 ,  B01L3/00 ,  G01N27/447

CPC classification number: B01L3/50273 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0858 ,  B01L2300/0877 ,  B01L2300/12 ,  B01L2300/165 ,  B01L2400/0427 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44791

Abstract: Single-sided optoelectrowetting (SSOEW)-configured substrates are provided, as well as microfluidic devices that include such substrates. The substrates can include a planar electrode, a photoconductive (or photosensitive) layer, a dielectric layer (single-layer or composite), a mesh electrode, and a hydrophobic coating. Fluid droplets can be moved across the hydrophobic coating of such substrates in a light-actuated manner, upon the application of a suitable AC voltage potential across the substrate and the focusing of light into the photoconductive layer of the substrate in a location proximal to the droplets. Walls can be disposed upon the substrates to form the microfluidic devices. Together the walls and substrate can form a microfluidic circuit, through which droplets can be moved.

公开(公告)号：US20140017791A1

公开(公告)日：2014-01-16

申请号：US13940424

申请日：2013-07-12

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Igor Y. Khandros ,  Gaetan L. Mathieu ,  Steven W. Short ,  Ming C. Wu

IPC: C12N13/00

CPC classification number: C12N13/00 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0454 ,  C12N15/02

Abstract: Two or more biological micro-objects can be grouped in a liquid medium in a chamber. Grouping can comprise bringing into and holding in proximity or contact the micro-objects in a group, breaching the membrane of one or more of the micro-objects in a group, subjecting one or more of the micro-objects in a group to electroporation, and/or tethering to each other the micro-objects in a group. The micro-objects in the group can then be combined into a single biological object.

Abstract translation: 两个或更多个生物微物体可以分组在室中的液体介质中。 分组可以包括进入和保持接近或接触组中的微物体，破坏组中的一个或多个微物体的膜，使一组中的一个或多个微物体进行电穿孔， 和/或彼此束缚在一组中的微物体。 然后，组中的微物体可以组合成单个生物体。

公开(公告)号：US20130319861A1

公开(公告)日：2013-12-05

申请号：US13856781

申请日：2013-04-04

Applicant: BERKELEY LIGHTS, INC.

Inventor： Igor Y. Khandros ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Ming C. Wu

IPC: B81B1/00 ,  B03C5/02 ,  B03C5/00

CPC classification number: B81B1/00 ,  B01L3/0268 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0874 ,  B01L2400/0439 ,  B01L2400/0454 ,  B01L2400/0481 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26

Abstract: A micro-fluidic device can include a processing mechanism for processing micro-objects in a liquid medium and an outputting mechanism for expressing from the device a droplet of the medium containing one or more of the micro-objects. The outputting mechanism can include an expressing mechanism having a reservoir for holding a quantity of the liquid medium and a striking mechanism for striking and compressing the expressing mechanism to express a droplet of the medium from the expressing mechanism.

Abstract translation: 微流体装置可以包括用于处理液体介质中的微物体的处理机构和用于从装置表达包含一个或多个微物体的介质的液滴的输出机构。 输出机构可以包括具有用于保持一定量的液体介质的储存器的表达机构和用于敲击和压缩表达机构以从表达机构表达介质液滴的打击机构。