公开(公告)号：US12030051B2

公开(公告)日：2024-07-09

申请号：US16419485

申请日：2019-05-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Kyle Gleason ,  Austin Payne ,  Priyadarshini Gogoi ,  Christopher Siemer ,  Yi Zhou ,  Saedeh Javdani Sepehri

IPC: B01L3/00 ,  B01L7/00 ,  G01N35/00

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/527 ,  B01L3/5635 ,  B01L7/00 ,  G01N35/0098 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0684 ,  B01L2300/06 ,  B01L2300/0816 ,  B01L2300/0832 ,  B01L2300/0864 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/185 ,  B01L2400/0487 ,  B01L2400/0683

Abstract: A system for isolating cells in at least one of single-cell format and single-cluster format, comprising a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive a biological sample and to receive at least one fluid, a manifold configured to receive and deliver the biological sample and the at least one fluid from the reservoir into a biological sample substrate, the manifold comprising a broad surface comprising a central region configured to receive the biological sample substrate, a set of openings configured to enable fluid flow transmission across the biological sample substrate, a manifold inlet configured to transmit flow from the reservoir the first subset of openings, a manifold outlet configured at a downstream end of the broad surface and coupled to the second subset of openings, the manifold outlet configured to transmit waste fluid from the manifold.

公开(公告)号：US20230015223A1

公开(公告)日：2023-01-19

申请号：US17951719

申请日：2022-09-23

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  G01N1/20 ,  G01N1/40 ,  G01N1/28 ,  C12M1/00 ,  B01L3/00 ,  G01N35/10 ,  G01N35/00 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20210283609A1

公开(公告)日：2021-09-16

申请号：US17333337

申请日：2021-05-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Saedeh Javdani Sepehri ,  Kyle Gleason

IPC: B01L3/00 ,  G01N15/10 ,  G01N15/14 ,  C12M1/00 ,  C12Q1/6834 ,  C12Q1/6841 ,  G01N33/574

Abstract: A system and method for isolating and analyzing single cells, comprising: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20240255412A1

公开(公告)日：2024-08-01

申请号：US18633224

申请日：2024-04-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  William Chow

IPC: G01N15/14 ,  B01L3/00 ,  B01L3/02 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/01 ,  G01N15/10 ,  G01N15/1433 ,  G01N15/149 ,  G01N35/00 ,  G01N35/10 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69

CPC classification number: G01N15/1484 ,  B01L3/502715 ,  B01L3/502761 ,  C12M47/04 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N1/405 ,  G01N1/4077 ,  G01N15/1433 ,  G01N35/00029 ,  G01N35/1011 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69 ,  B01L3/021 ,  B01L3/502746 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/086 ,  G01N15/01 ,  G01N2015/1006 ,  G01N15/149 ,  G01N2035/00158 ,  G01N2035/1039

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20240253038A1

公开(公告)日：2024-08-01

申请号：US18233819

申请日：2023-08-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC: B01L3/00 ,  C12M1/00 ,  C12Q1/6816 ,  C12Q1/6886 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1434 ,  G01N15/149 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574

CPC classification number: B01L3/502761 ,  C12M47/04 ,  C12Q1/6816 ,  G01N15/1434 ,  G01N15/1484 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574 ,  B01L3/502715 ,  B01L3/527 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2400/0406 ,  C12Q1/6886 ,  G01N2015/1006 ,  G01N15/149

Abstract: A system and method, wherein the method includes: (a) receiving a fluid comprising a process reagent for an assay and nucleic acid material from a sample into a fluid pathway coupled to an array of wells, wherein the array of wells is defined within a substrate and each well in the array of wells extends vertically into a planar surface of the substrate; (b) distributing the fluid along the fluid pathway and into wells of the array of wells; and (c) distributing a liquid immiscible with the fluid comprising the process reagent and nucleic acid material along the fluid pathway upon positive pressure generation by a pump of a flow control system in communication with an inlet to the fluid pathway, thereby displacing said fluid along the fluid pathway and into wells of the array of wells and preventing contents of each well from transferring to adjacent wells.

公开(公告)号：US11865542B2

公开(公告)日：2024-01-09

申请号：US17965495

申请日：2022-10-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC: B01L3/00 ,  C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  G01N33/543 ,  G01N33/569 ,  G01N15/14 ,  C12Q1/6886 ,  G01N15/10

CPC classification number: B01L3/502761 ,  C12M47/04 ,  C12Q1/6816 ,  G01N15/1434 ,  G01N15/1484 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574 ,  B01L3/502715 ,  B01L3/527 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2400/0406 ,  C12Q1/6886 ,  G01N2015/1006 ,  G01N2015/149

Abstract: A system and method, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity and a fluid delivery module including a fluid pathway through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: generating a set of genetic complexes within individual wells of the array of wells.

公开(公告)号：US11358146B2

公开(公告)日：2022-06-14

申请号：US17333142

申请日：2021-05-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC: C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  C12Q1/6886 ,  G01N15/10 ,  B01L3/00 ,  G01N33/543 ,  G01N33/569 ,  G01N15/14

Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir through which fluid flow is controlled along a fluid path; and wherein the method includes: capturing a population of particles into the array of wells in single-particle format; releasing, from the particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

公开(公告)号：US11052396B2

公开(公告)日：2021-07-06

申请号：US16673416

申请日：2019-11-04

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Saedeh Javdani Sepehri ,  Kyle Gleason

IPC: B01L3/00 ,  G01N15/10 ,  G01N15/14 ,  C12M1/00 ,  C12Q1/6834 ,  C12Q1/6841 ,  G01N33/574 ,  G01N15/00

Abstract: A system and method for isolating and analyzing single cells, comprising: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20210003496A1

公开(公告)日：2021-01-07

申请号：US17025326

申请日：2020-09-18

Applicant: Bio-Rad Laboratories, Inc

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  G01N1/20 ,  G01N1/40 ,  G01N1/28 ,  C12M1/00 ,  B01L3/00 ,  G06K9/20 ,  G01N35/10 ,  G06K9/00 ,  G01N35/00

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20250020574A1

公开(公告)日：2025-01-16

申请号：US18887450

申请日：2024-09-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  B01L3/00 ,  B01L3/02 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/01 ,  G01N15/10 ,  G01N15/1433 ,  G01N15/149 ,  G01N35/00 ,  G01N35/10 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.