公开(公告)号：US12152980B2

公开(公告)日：2024-11-26

申请号：US18633224

申请日：2024-04-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  B01L3/00 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/1433 ,  G01N35/00 ,  G01N35/10 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69 ,  B01L3/02 ,  G01N15/01 ,  G01N15/10 ,  G01N15/149

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US12044614B2

公开(公告)日：2024-07-23

申请号：US17589507

申请日：2022-01-31

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  B01L3/00 ,  B01L3/02 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/1433 ,  G01N35/00 ,  G01N35/10 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69 ,  G01N15/00 ,  G01N15/01 ,  G01N15/10

CPC classification number: G01N15/1484 ,  B01L3/502715 ,  B01L3/502761 ,  C12M47/04 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N1/405 ,  G01N1/4077 ,  G01N15/1433 ,  G01N35/00029 ,  G01N35/1011 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69 ,  B01L3/021 ,  B01L3/502746 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/086 ,  G01N15/01 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2035/00158 ,  G01N2035/1039

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US11866766B2

公开(公告)日：2024-01-09

申请号：US18048512

申请日：2022-10-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  John Stark ,  Yadwinder Deol ,  Vishal Sharma

IPC: C12Q1/6811 ,  C12Q1/6874 ,  C12N15/10

CPC classification number: C12Q1/6811 ,  C12N15/1065 ,  C12Q1/6874 ,  C12Q2563/179 ,  C12Q2565/629 ,  C12Q2600/178

Abstract: A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.

公开(公告)号：US20230015223A1

公开(公告)日：2023-01-19

申请号：US17951719

申请日：2022-09-23

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  G01N1/20 ,  G01N1/40 ,  G01N1/28 ,  C12M1/00 ,  B01L3/00 ,  G01N35/10 ,  G01N35/00 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20220152606A1

公开(公告)日：2022-05-19

申请号：US17590543

申请日：2022-02-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Swati Ranade ,  Vishal Sharma ,  Austin Payne ,  Sam Tuck

IPC: B01L3/00 ,  C12N13/00

Abstract: A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).

公开(公告)号：US11273439B2

公开(公告)日：2022-03-15

申请号：US16906337

申请日：2020-06-19

Applicant: Bio-Rad Laboratories, Inc

Inventor： Kalyan Handique ,  Swati Ranade ,  Vishal Sharma ,  Austin Payne ,  Sam Tuck

IPC: B01L3/00 ,  C12N13/00

Abstract: A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).

公开(公告)号：US20230407372A1

公开(公告)日：2023-12-21

申请号：US18242658

申请日：2023-09-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  John Stark ,  Yadwinder Deol ,  Vishal Sharma

IPC: C12Q1/6811 ,  C12Q1/6874 ,  C12N15/10

CPC classification number: C12Q1/6811 ,  C12Q1/6874 ,  C12Q2563/179 ,  C12Q2565/629 ,  C12Q2600/178 ,  C12N15/1065

Abstract: A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.

公开(公告)号：US20230182127A1

公开(公告)日：2023-06-15

申请号：US18076667

申请日：2022-12-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Vishal Sharma ,  Ronald Lebofsky ,  Man Cheng ,  Adam McCoy ,  Kalyan Handique ,  Eric Pomaranski

IPC: B01L3/00 ,  C12N13/00

CPC classification number: B01L3/502 ,  C12N13/00 ,  B01L2200/026 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/047 ,  B01L2300/0893

Abstract: Inventions covered include methods, systems, and compositions for sample processing, involving morphology-adjustable (e.g., tunable on-demand) functionalized particles. In some embodiments, a method can include distributing a set of functionalized particles, in a first morphological state, across a set of partitions; transitioning the set of functionalized particles, at the set of partitions, from the first morphological state to a second morphological state; transitioning the set of functionalized particles, at the set of partitions, from the second morphological state to a third morphological state, and inducing interactions between the set of functionalized particles and a set of targets, within the set of partitions and according to a set of operations with a set of process fluids.

公开(公告)号：US20210283608A1

公开(公告)日：2021-09-16

申请号：US17333142

申请日：2021-05-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC: B01L3/00 ,  C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  G01N33/543 ,  G01N33/569 ,  G01N15/14

Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

公开(公告)号：US20210171939A1

公开(公告)日：2021-06-10

申请号：US17109704

申请日：2020-12-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam McCoy ,  Kalyan Handique ,  Jonathan Bell ,  Vishal Sharma ,  Sida Wang

IPC: C12N15/10 ,  C12Q1/6853 ,  C12Q1/686

Abstract: An embodiment of a composition for target material separation includes: a body and one or more molecules coupled to the body and structured for functionalization of the composition. In embodiments, each of the one or more molecules can include one or more of: a linker region; a polymerase chain reaction (PCR) segment or oligo binding region; one or more barcode region(s); a unique molecule identifier; a preparation-facilitating segment; an active segment; and a molecular scissor or cleavage region, wherein various regions can be coupled together in order to provide functionality to the composition. The invention(s) also cover manufacturing of the composition and various applications of use, in the context of target material capture (e.g., from single cells or other biological material).