公开(公告)号：US09835539B2

公开(公告)日：2017-12-05

申请号：US14749202

申请日：2015-06-24

Applicant: International Business Machines Corporation

Inventor： Yann A. Astier ,  Joshua T. Smith ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Benjamin H. Wunsch

IPC: G01N15/10 ,  G01N27/327 ,  G01N33/487 ,  G01N15/02 ,  G01N15/00 ,  B01L3/00

CPC classification number: G01N15/1031 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0864 ,  B01L2300/161 ,  G01N15/02 ,  G01N27/3278 ,  G01N33/48721 ,  G01N2015/0038 ,  G01N2015/0288

Abstract: A technique relates sorting biopolymers. The biopolymers are introduced into a nanopillar array, and the biopolymers include a first population and a second population. The nanopillar array includes nanopillars arranged to have a gap separating one from another. The biopolymers are sorted through the nanopillar array by transporting the first population of the biopolymers less than a predetermined bumping size according to a fluid flow direction and by transporting the second population of the biopolymers at least the predetermined bumping size according to a bumped direction different from the fluid flow direction. The nanopillar array is configured to employ the gap with a gap size less than 300 nanometers in order to sort the biopolymers.

公开(公告)号：US09835538B2

公开(公告)日：2017-12-05

申请号：US14697269

申请日：2015-04-27

Applicant: International Business Machines Corporation

Inventor： Yann A. Astier ,  Joshua T. Smith ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Benjamin H. Wunsch

IPC: G01N15/10 ,  G01N27/327 ,  G01N33/487 ,  G01N15/02 ,  G01N15/00 ,  B01L3/00

CPC classification number: G01N15/1031 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0864 ,  B01L2300/161 ,  G01N15/02 ,  G01N27/3278 ,  G01N33/48721 ,  G01N2015/0038 ,  G01N2015/0288

Abstract: A technique relates sorting biopolymers. The biopolymers are introduced into a nanopillar array, and the biopolymers include a first population and a second population. The nanopillar array includes nanopillars arranged to have a gap separating one from another. The biopolymers are sorted through the nanopillar array by transporting the first population of the biopolymers less than a predetermined bumping size according to a fluid flow direction and by transporting the second population of the biopolymers at least the predetermined bumping size according to a bumped direction different from the fluid flow direction. The nanopillar array is configured to employ the gap with a gap size less than 300 nanometers in order to sort the biopolymers.

公开(公告)号：US20170329914A1

公开(公告)日：2017-11-16

申请号：US15151501

申请日：2016-05-11

Applicant: International Business Machines Corporation

Inventor： Solomon Assefa ,  Geoffrey H. Siwo ,  Gustavo A. Stolovitzky

IPC: G06F19/00 ,  G06F19/18 ,  G06F19/24

CPC classification number: G16H50/20 ,  G06F19/324 ,  G16B20/00 ,  G16B40/00

Abstract: Embodiments of the present invention may provide the capability to predict the metastasis of cancer in a patient from one tissue to another. In an embodiment, a computer-implemented method for predicting metastasis may comprise receiving an indication of at least one disrupted gene of the cancer, traversing data representing a gene-to-gene or protein-to-protein interaction network specific for a type of the cancer type from a position of the received gene in the network to a position of at least one gene involved in metastasis for a tissue type, organ or body part, determining at least one shortest path in the network between the received gene and the at least one gene involved in metastasis for the tissue type, organ or body part, generating a prediction of metastasis to the tissue type based on the at least one determined path, and generating an output display indicating a likelihood of spread of cancer to the tissue type, organ or body part.

公开(公告)号：US09776184B2

公开(公告)日：2017-10-03

申请号：US15074701

申请日：2016-03-18

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Qinghuang Lin ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Deqiang Wang

IPC: B01L3/00 ,  G01N27/447 ,  G01N33/487

CPC classification number: G01N33/48721 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2200/12 ,  B01L2300/042 ,  B01L2300/0645 ,  B01L2300/0887 ,  B01L2300/0896 ,  B01L2300/161 ,  B01L2400/0415 ,  B01L2400/0421 ,  G01N27/447 ,  G01N27/44791 ,  Y10T29/49002

Abstract: A device for passing a biopolymer molecule includes a nanochannel formed between a surface relief structure, a patterned layer forming sidewalls of the nanochannel and a sealing layer formed over the patterned layer to encapsulate the nanochannel. The surface relief structure includes a three-dimensionally rounded surface that reduces a channel dimension of the nanochannel at a portion of nanochannel and gradually increases the dimension along the nanochannel toward an opening position, which is configured to receive a biopolymer.

公开(公告)号：US20170059548A1

公开(公告)日：2017-03-02

申请号：US15349285

申请日：2016-11-11

Applicant: International Business Machines Corporation

Inventor： Ali Afzali-Ardakani ,  Stefan Harrer ,  Binquan Luan ,  Hongbo Peng ,  Stephen M. Rossnagel ,  Ajay K. Royyuru ,  Gustavo A. Stolovitzky ,  Philip S. Waggoner

IPC: G01N33/487 ,  G01N27/447 ,  C12Q1/68

CPC classification number: G01N33/48721 ,  B82Y15/00 ,  C12Q1/6869 ,  G01N27/44743 ,  G01N27/44791 ,  Y10S977/924 ,  C12Q2563/157

Abstract: A nanodevice includes a reservoir filled with conductive fluid and a membrane separating the reservoir. A nanopore is formed through the membrane having electrode layers separated by insulating layers. A certain electrode layer has a first type of organic coating and a pair of electrode layers has a second type. The first type of organic coating forms a motion control transient bond to a molecule in the nanopore for motion control, and the second type forms first and second transient bonds to different bonding sites of a base of the molecule. When a voltage is applied to the pair of electrode layers a tunneling current is generated by the base in the nanopore, and the tunneling current travels via the first and second transient bonds formed to be measured as a current signature for distinguishing the base. The motion control transient bond is stronger than first and second transient bonds.

Abstract translation: 纳米器件包括填充有导电流体的储存器和分离储存器的膜。 通过具有由绝缘层分隔的电极层的膜形成纳米孔。 某一电极层具有第一类型的有机涂层，并且一对电极层具有第二类型。 第一类型的有机涂层形成运动控制瞬态键合到用于运动控制的纳米孔中的一个分子，而第二种类型形成与分子的基底的不同键合位点的第一和第二瞬态键。 当对一对电极层施加电压时，由纳米孔中的基底产生隧穿电流，并且隧穿电流通过形成为待测量的第一和第二瞬态键作为用于区分基极的电流签名行进。 运动控制瞬态键比第一和第二瞬态键强。

公开(公告)号：US20160146778A1

公开(公告)日：2016-05-26

申请号：US14697269

申请日：2015-04-27

Applicant: International Business Machines Corporation

Inventor： Yann A. Astier ,  Joshua T. Smith ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Benjamin H. Wunsch

IPC: G01N33/487 ,  G01N27/327 ,  G01N15/10

CPC classification number: G01N15/1031 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0864 ,  B01L2300/161 ,  G01N15/02 ,  G01N27/3278 ,  G01N33/48721 ,  G01N2015/0038 ,  G01N2015/0288

Abstract: A technique relates sorting biopolymers. The biopolymers are introduced into a nanopillar array, and the biopolymers include a first population and a second population. The nanopillar array includes nanopillars arranged to have a gap separating one from another. The biopolymers are sorted through the nanopillar array by transporting the first population of the biopolymers less than a predetermined bumping size according to a fluid flow direction and by transporting the second population of the biopolymers at least the predetermined bumping size according to a bumped direction different from the fluid flow direction. The nanopillar array is configured to employ the gap with a gap size less than 300 nanometers in order to sort the biopolymers.

公开(公告)号：US09222930B2

公开(公告)日：2015-12-29

申请号：US13971532

申请日：2013-08-20

Applicant: International Business Machines Corporation

Inventor： Hongbo Peng ,  Stephen M. Rossnagel ,  Ajay K. Royyuru ,  Gustavo A. Stolovitzky ,  Deqiang Wang

IPC: H01B13/00 ,  G01N33/487 ,  C12Q1/68 ,  C25D7/12 ,  C23C18/16 ,  C25D5/02 ,  C25D7/00 ,  C25D3/50 ,  C25D21/12 ,  C23C18/42

CPC classification number: G01N33/48721 ,  C12Q1/6869 ,  C23C18/161 ,  C23C18/1644 ,  C23C18/1675 ,  C23C18/42 ,  C25D3/50 ,  C25D5/02 ,  C25D7/00 ,  C25D7/12 ,  C25D21/12

Abstract: A mechanism is provided for forming a nanodevice. A reservoir is filled with a conductive fluid, and a membrane is formed to separate the reservoir in the nanodevice. The membrane includes an electrode layer having a tunneling junction formed therein. The membrane is formed to have a nanopore formed through one or more other layers of the membrane such that the nanopore is aligned with the tunneling junction of the electrode layer. The tunneling junction of the electrode layer is narrowed to a narrowed size by electroplating or electroless deposition. When a voltage is applied to the electrode layer, a tunneling current is generated by a base in the tunneling junction to be measured as a current signature for distinguishing the base. When an organic coating is formed on an inside surface of the tunneling junction, transient bonds are formed between the electrode layer and the base.

Abstract translation: 提供了用于形成纳米器件的机构。 储存器填充有导电流体，并且形成膜以分离纳米装置中的储存器。 膜包括其中形成有隧道结的电极层。 膜形成为具有通过膜的一个或多个其它层形成的纳米孔，使得纳米孔与电极层的隧道结对准。 电极层的隧道结通过电镀或无电沉积而变窄到窄的尺寸。 当向电极层施加电压时，通过在要测量的隧道结中的基底产生隧道电流作为区分基底的当前签名。 当在隧道结的内表面上形成有机涂层时，在电极层和基底之间形成瞬态结合。

公开(公告)号：US08975095B2

公开(公告)日：2015-03-10

申请号：US13904403

申请日：2013-05-29

Applicant: International Business Machines Corporation

Inventor： Shu-Jen Han ,  Ajay K. Royyuru ,  Gustavo A. Stolovitzky ,  Deqiang Wang

IPC: H01L21/66 ,  C12Q1/68 ,  G01N27/414 ,  G01N27/447

CPC classification number: C12Q1/6869 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/48721 ,  Y10S977/924 ,  C12Q2563/116 ,  C12Q2563/157 ,  C12Q2565/631

Abstract: A technique is provided for base recognition in an integrated device is provided. A target molecule is driven into a nanopore of the integrated device. The integrated device includes a nanowire separated into a left nanowire part and a right nanowire part to form a nanogap in between, a source pad connected to the right nanowire part, a drain pad connected to the left nanowire part, and the nanopore. The source pad, the drain pad, the right nanowire part, the left nanowire part, and the nanogap together form a transistor. The nanogap is part of the nanopore. A transistor current is measured while a single base of the target molecule is in the nanogap of the nanopore, and the single base affects the transistor current. An identity of the single base is determined according to a change in the transistor current.

Abstract translation: 提供了一种用于集成设备中的基础识别的技术。 靶分子被驱动到集成器件的纳米孔中。 集成器件包括分离成左纳米线部分的纳米线和右纳米线部分，以在其间形成纳米间隙，连接到右纳米线部分的源极焊盘，连接到左纳米线部分的漏极焊盘和纳米孔。 源极焊盘，漏极焊盘，右侧的纳米线部分，左侧的纳米线部分和纳米光栅一起形成晶体管。 纳米孔是纳米孔的一部分。 测量晶体管电流，同时目标分子的单个碱基位于纳米孔的纳米隙中，单个碱基影响晶体管电流。 根据晶体管电流的变化确定单个基极的身份。

公开(公告)号：US20150037843A1

公开(公告)日：2015-02-05

申请号：US14012665

申请日：2013-08-28

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Binquan Luan ,  Ajay K. Royyuru ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Deqiang Wang

IPC: C12P19/34

CPC classification number: C12P19/34 ,  C12N15/11 ,  C12Q1/6806 ,  Y10T436/143333 ,  C12Q2565/131 ,  C12Q2565/631

Abstract: A mixed polynucleotide includes a first double stranded (ds) portion, a second portion including at least one single stranded (ss) portion, and a third ds portion. The second portion connects the first ds portion and the third ds portion to provide a modified polynucleotide.

Abstract translation: 混合多核苷酸包括第一双链（ds）部分，第二部分包括至少一个单链（ss）部分和第三ds部分。 第二部分连接第一个ds部分和第三个ds部分，以提供修饰的多核苷酸。

公开(公告)号：US20140326604A1

公开(公告)日：2014-11-06

申请号：US13904403

申请日：2013-05-29

Applicant: International Business Machines Corporation

Inventor： Shu-Jen Han ,  Ajay K. Royyuru ,  Gustavo A. Stolovitzky ,  Deqiang Wang

IPC: C12Q1/68 ,  G01N27/447

CPC classification number: C12Q1/6869 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/48721 ,  Y10S977/924 ,  C12Q2563/116 ,  C12Q2563/157 ,  C12Q2565/631

Abstract: A technique is provided for base recognition in an integrated device is provided. A target molecule is driven into a nanopore of the integrated device. The integrated device includes a nanowire separated into a left nanowire part and a right nanowire part to form a nanogap in between, a source pad connected to the right nanowire part, a drain pad connected to the left nanowire part, and the nanopore. The source pad, the drain pad, the right nanowire part, the left nanowire part, and the nanogap together form a transistor. The nanogap is part of the nanopore. A transistor current is measured while a single base of the target molecule is in the nanogap of the nanopore, and the single base affects the transistor current. An identity of the single base is determined according to a change in the transistor current.

Abstract translation: 提供了一种用于集成设备中的基础识别的技术。 靶分子被驱动到集成器件的纳米孔中。 集成器件包括分离成左纳米线部分的纳米线和右纳米线部分，以在其间形成纳米间隙，连接到右纳米线部分的源极焊盘，连接到左纳米线部分的漏极焊盘和纳米孔。 源极焊盘，漏极焊盘，右侧的纳米线部分，左侧的纳米线部分和纳米光栅一起形成晶体管。 纳米孔是纳米孔的一部分。 测量晶体管电流，同时目标分子的单个碱基位于纳米孔的纳米隙中，单个碱基影响晶体管电流。 根据晶体管电流的变化确定单个基极的身份。