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公开(公告)号：US5411873A
公开(公告)日：1995-05-02
申请号：US928697
申请日：1992-08-11
摘要： Processes for producing various heterologous polypeptides which when expressed are either incorrectly processed and hence asssociated with the surface of the host cell or are not processed to mature form. More specifically, processes for the production of heterologous non-human carbonyl hydrolases expressed either in host cells incapable of producing enzymatically active endoprotease or host cells deficient in enzymatically active extracellular endoprotease are disclosed. Such non-human carbonyl hydrolases generally are incapable of autoproteolytic maturation and become associated with the surface of expression hosts which are deficient in enzymatically active extracellular endoprotease. Processes for preparing non-human carbonyl hydrolase and heterologous polypeptides which are expressed as part of a fusion polypeptide are also disclosed, as well as non-human carbonyl hydrolases which are substantially free of the host cell membrane with which they are normally associated.
摘要翻译： 用于产生各种异源多肽的方法，当被表达时，它们被错误地加工并因此与宿主细胞的表面相结合或者不被加工成成熟形式。 更具体地，公开了在不能产生酶促活性内切蛋白酶的宿主细胞中表达的异源非人羰基水解酶的生产方法或在酶活性细胞外内蛋白酶中缺乏的宿主细胞。 这种非人羰基水解酶通常不能自身蛋白水解成熟，并且与酶活性细胞外内蛋白酶缺陷的表达宿主的表面相关联。 还公开了制备非人羰基水解酶和作为融合多肽的一部分表达的异源多肽的方法，以及基本上不含与它们通常结合的宿主细胞膜的非人羰基水解酶。
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公开(公告)号：US4803164A
公开(公告)日：1989-02-07
申请号：US42604
申请日：1987-04-22
CPC分类号： C07K14/005 , A61K39/12 , A61K39/292 , C12N15/81 , C12N2730/10122 , C12N2730/10134
摘要： Hepatitis surface antigen is synthesized in recombinant yeast hosts transformed with vectors encoding hepatitis surface antigen, preferably under the control of the yeast PGK promoter and preferably in the absence of DNA encoding the surface antigen precursor. Hepatitis surface antigen is assembled by yeast into antigenic 22 nm particles even though hepatitis surface antigen bacterial transformants were not known to be capable of assembling the surface antigen into 22 nm particles.
摘要翻译： 在用编码肝炎表面抗原的载体转化的重组酵母宿主中合成肝炎表面抗原，优选在酵母PGK启动子的控制下，优选在不存在编码表面抗原前体的DNA的情况下。 尽管肝炎表面抗原细菌转化体不能将表面抗原组装成22纳米的颗粒，但肝炎表面抗原由酵母组装成抗原性22nm的颗粒。
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公开(公告)号：US20110200596A1
公开(公告)日：2011-08-18
申请号：US13045316
申请日：2011-03-10
IPC分类号： A61K39/395 , C07K16/28 , C12P21/00 , A61P35/00
CPC分类号： C07K16/283 , A61K2039/505 , C07K16/00 , C07K16/2863 , C07K2317/14 , C07K2317/24 , C07K2317/41 , C07K2317/52 , C07K2317/524 , C07K2317/526 , C07K2317/53 , C07K2317/55 , C07K2317/56 , C07K2317/565 , C07K2317/622 , C07K2317/73 , C07K2317/76 , C07K2317/94 , C07K2319/00
摘要： The invention provides methods and compositions comprising a novel stabilized monovalent antibody fragment.
摘要翻译： 本发明提供了包含新的稳定的单价抗体片段的方法和组合物。
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公开(公告)号：US06242177B1
公开(公告)日：2001-06-05
申请号：US08397303
申请日：1995-03-01
IPC分类号： C12Q168
CPC分类号： C12N15/625 , C07K2319/02 , C07K2319/036 , C07K2319/61 , C07K2319/75 , C12N15/70
摘要： The instant invention discloses the unexpected result that mutant signal sequences with reduced translational strength provided essentially complete processing and high levels of expression of a polypeptide of interest as compared to wild type signal sequences, and that many mammalian polypeptides require a narrow range of translation levels to achieve maximum secretion. A set of signal sequence vectors provides a range of translational strengths for optimizing expression of a polypeptide of interest.
摘要翻译： 本发明公开了意想不到的结果，与野生型信号序列相比，具有降低的翻译强度的突变信号序列提供了基本上完整的处理和高水平的感兴趣的多肽的表达，并且许多哺乳动物多肽需要较窄的翻译水平范围 达到最大分泌。 一组信号序列载体提供一系列翻译强度，以优化感兴趣的多肽的表达。
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公开(公告)号：US4912046A
公开(公告)日：1990-03-27
申请号：US62318
申请日：1987-06-08
摘要： Shuttle vector systems are provided for the regulated expression of subject genes in transformed hosts. A promoter/operator which does not originate with the transformed host cell controls expression of the subject gene and a repressor, which also does not originate with the host cell, regulates the promoter/operator. According to this invention, promoter/operator--repressor functionalities from one bacterium are used in other bacteria, thereby obviating the need to locate homologous expression regulatory sequences for each bacterial host.
摘要翻译： 提供穿梭载体系统用于受体基因在转化宿主中的调节表达。 不起源于转化的宿主细胞的启动子/操纵子控制受试者基因的表达和也不是源自宿主细胞的阻遏物调节启动子/操纵子。 根据本发明，来自一种细菌的启动子/操纵子阻遏物官能团用于其它细菌，从而避免需要定位每个细菌宿主的同源表达调控序列。
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公开(公告)号：US4741901A
公开(公告)日：1988-05-03
申请号：US603529
申请日：1984-04-24
IPC分类号： A61K39/00 , A61K39/29 , C07K14/02 , A61K39/12 , C07K13/00 , C12N1/00 , C12N5/00 , C12N15/00 , C12P21/00 , C12P21/02 , C12P21/04
CPC分类号： C07K14/005 , A61K39/12 , A61K39/292 , A61K2039/55555 , A61K39/00 , C12N2730/10122 , C12N2730/10134 , Y10S530/806 , Y10S530/826
摘要： Novel vaccines are provided for immunization against hepatitis B surface antigen wherein the surface antigen is present in 22nm form but contains only mature hepatitis B surface antigen.
摘要翻译： 提供新型疫苗用于免疫乙型肝炎表面抗原，其中表面抗原以22nm形式存在，但仅含有成熟乙型肝炎表面抗原。
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公开(公告)号：US09637557B2
公开(公告)日：2017-05-02
申请号：US13092708
申请日：2011-04-22
CPC分类号： C07K16/468 , A61K2039/505 , C07K16/244 , C07K16/247 , C07K16/2863 , C07K16/30 , C07K2317/31 , C07K2317/526 , C07K2317/73 , C07K2317/76 , C07K2317/90 , C07K2317/92 , Y02A50/41
摘要： Described herein are methods for the efficient production of antibodies and other multimeric protein complexes (collectively referred to herein as heteromultimeric proteins) capable of specifically binding to more than one target. The targets may be, for example, different epitopes on a single molecule or located on different molecules. The methods combine efficient, high gene expression level, appropriate assembly, and ease of purification for the heteromultimeric proteins. The invention also provides methods of using these heteromultimeric proteins, and compositions, kits and articles of manufacture comprising these antibodies.
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18.发明授权
公开(公告)号：US5888808A
公开(公告)日：1999-03-30
申请号：US55960
申请日：1993-04-29
IPC分类号： C12N15/00 , A61K38/00 , A61K38/27 , A61K38/28 , A61K39/00 , A61K39/395 , C07D249/08 , C07D253/06 , C07D501/20 , C07H21/00 , C07H21/04 , C07K14/61 , C07K14/655 , C12N20060101 , C12N1/20 , C12N15/09 , C12N15/10 , C12N15/62 , C12N15/66 , C12N15/71 , C12P19/34 , C12P21/00 , C12Q1/68 , C12N15/63 , C12N15/70
CPC分类号： C12N15/10 , C07K14/61 , C07K14/655 , C12N15/62 , C12N15/71 , C12P19/34 , C07K2319/00 , C07K2319/02 , C07K2319/75
摘要： The present invention provides recombinant DNA vehicles which are suitable for the microbial expression of DNA encoding a heterologous polypeptide which comprises a portion of the trp operon having the promoter-operator and leader ribosome binding site, and a restriction site providing an insertion site for the DNA sequences encoding the heterologous polypeptide, wherein the restriction site is located 3' of the leader ribosome binding site as a substitute for the Taq I site of the trp promoter-operator and is selected from the group consisting of Xba I and Eco RI. Also provided are E. coli strains transformed with the above described recombinant DNA vehicles.
摘要翻译： 本发明提供了适用于编码异源多肽的DNA的微生物表达的重组DNA载体，其包含具有启动子操纵子和前导核糖体结合位点的一部分trp操纵子，以及提供DNA插入位点的限制性位点 编码异源多肽的序列，其中限制性位点位于前导核糖体结合位点的3'，作为trp启动子 - 操纵子的Taq I位点的替代物，并且选自Xba I和Eco RI。 还提供用上述重组DNA载体转化的大肠杆菌菌株。
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公开(公告)号：US4663283A
公开(公告)日：1987-05-05
申请号：US543682
申请日：1983-10-20
IPC分类号： C07K14/61 , C07K14/655 , C12N15/10 , C12N15/66 , C12N15/71 , C12P21/02 , C12P19/34 , C12N15/00
CPC分类号： C12N15/71 , C07K14/61 , C07K14/655 , C12N15/10 , C12N15/66 , C12P21/02 , C07K2319/00 , C07K2319/02 , C07K2319/75
摘要： A method of altering double stranded DNA comprising converting double stranded DNA to single stranded DNA in a region surrounding a selected cleavage site, hybridizing a complimentary primer to the single stranded region formed in the previous step, and restoring enzymatically the second strand which had been eliminated.
摘要翻译： 一种改变双链DNA的方法，包括在选定的切割位点周围的区域中将双链DNA转化为单链DNA，将互补引物与前述步骤中形成的单链区域杂交，并且酶消化已经被消除的第二链 。
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公开(公告)号：US20110287009A1
公开(公告)日：2011-11-24
申请号：US13092708
申请日：2011-04-22
IPC分类号： A61K39/395 , C40B50/06 , C07K1/22 , C12P21/00 , C07K1/14
CPC分类号： C07K16/468 , A61K2039/505 , C07K16/244 , C07K16/247 , C07K16/2863 , C07K16/30 , C07K2317/31 , C07K2317/526 , C07K2317/73 , C07K2317/76 , C07K2317/90 , C07K2317/92 , Y02A50/41
摘要： Described herein are methods for the efficient production of antibodies and other multimeric protein complexes (collectively referred to herein as heteromultimeric proteins) capable of specifically binding to more than one target. The targets may be, for example, different epitopes on a single molecule or located on different molecules. The methods combine efficient, high gene expression level, appropriate assembly, and ease of purification for the heteromultimeric proteins. The invention also provides methods of using these heteromultimeric proteins, and compositions, kits and articles of manufacture comprising these antibodies.
摘要翻译： 本文描述了有效制备能够特异性结合多于一种靶的抗体和其它多聚体蛋白复合物（统称为异多聚体蛋白质）的方法。 靶可以是例如在单个分子上或位于不同分子上的不同表位。 该方法结合了有效，高基因表达水平，合适的组装和异源多聚体蛋白的纯化方便。 本发明还提供了使用这些异源多聚体蛋白质的方法，以及包含这些抗体的组合物，试剂盒和制品。
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