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11.发明授权Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator 有权使用物理或化学特征的气泡形成技术来保持液滴致动器内的气泡公开(公告)号：US08877512B2
公开(公告)日：2014-11-04
申请号：US12692954
申请日：2010-01-25
CPC分类号： C07K1/26 , B01F13/0076 , B01F13/0084 , B01F13/0086 , B01L3/502723 , B01L3/502738 , B01L3/502792 , B01L2200/0673 , B01L2300/0867 , B01L2300/089 , B01L2400/0406 , B01L2400/0427 , B01L2400/0688 , G01N2001/2244 , G01N2035/1034 , Y10T436/25
摘要： The present invention is directed to a droplet actuator and methods of making and using the droplet actuator including one or more substrates configured to form a droplet operations gap and including a physical or chemical feature that may be provided at a predetermined locus within or exposed to the droplet operations gap and configured to retain a bubble in position within the droplet operations gap.
摘要翻译： 本发明涉及液滴致动器以及制造和使用液滴致动器的方法，所述液滴致动器包括一个或多个被配置成形成液滴操作间隙的基板，并且包括物理或化学特征，该物理或化学特征可以设置在或 液滴操作间隙并且被配置为将气泡保持在液滴操作间隙内的适当位置。
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公开(公告)号：US08562807B2
公开(公告)日：2013-10-22
申请号：US12747231
申请日：2008-12-10
申请人： Vijay Srinivasan , Michael G. Pollack , Zhishan Hua , Arjun Sudarsan , Allen E. Eckhardt , Vamsee K. Pamula
发明人： Vijay Srinivasan , Michael G. Pollack , Zhishan Hua , Arjun Sudarsan , Allen E. Eckhardt , Vamsee K. Pamula
IPC分类号： G01N27/453
CPC分类号： G01N27/44743 , B01L3/0268 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/061 , B01L2200/0647 , B01L2300/0645 , B01L2300/16 , B01L2400/0415 , B01L2400/0427
摘要： Droplet actuators for conducting droplet operations, such as droplet transport and droplet dispensing, are provided. In one embodiment, the droplet actuator may include a substrate including, droplet operations electrodes arranged for conducting droplet operations on a surface of the substrate; and reference electrodes associated with the droplet operations electrodes and extending above the surface of the substrate. Other embodiments of droplet actuators and methods of loading and using such droplet actuators are also provided.
摘要翻译： 提供用于进行液滴操作的滴液致动器，例如液滴传输和液滴分配。 在一个实施例中，液滴致动器可以包括基板，其包括布置成在基板的表面上进行液滴操作的液滴操作电极; 以及与液滴操作电极相关并在衬底表面上方延伸的参考电极。 还提供了液滴致动器的其他实施例以及加载和使用这种液滴致动器的方法。
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公开(公告)号：US20110104747A1
公开(公告)日：2011-05-05
申请号：US12921256
申请日：2009-03-09
申请人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
发明人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
IPC分类号： G01N1/40
CPC分类号： B01L3/0282 , B01F13/002 , B01F13/0023 , B01F13/0071 , B01F13/0076 , B01F13/0818 , B01L3/502753 , B01L2200/0621 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2200/141 , B01L2300/0832 , B01L2300/0864 , B01L2300/0867 , B01L2400/043 , B03C1/01 , B03C1/284 , B03C1/286 , B03C2201/18 , B03C2201/26 , B03C2201/28 , Y10T436/25
摘要： Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.
摘要翻译： 提供了将珠子集中在流体装置上的液滴和/或负载珠粒中的方法，其中包括将珠粒集中在液滴中的方法，所述方法包括：（a）提供液滴致动器，其包括：（i） 内部液滴操作量; 和（ii）内部容积外部的储存器; （iii）建立在从储存器延伸到内部容积中的液体路径中的液滴; （b）在储存器中的液滴部分中提供磁响应珠; （c）通过所述液体路径将所述磁响应珠磁吸引到位于所述内部容积中的所述液滴的所述部分中; 和（d）在内部容积中形成包含一个或多个磁响应珠的液滴。
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公开(公告)号：US20100282608A1
公开(公告)日：2010-11-11
申请号：US12676384
申请日：2008-09-04
CPC分类号： B01L3/502792 , B01L2200/12 , B01L2300/0819 , B01L2300/089 , B01L2400/0415 , B01L2400/0421 , B01L2400/0427 , Y10T29/49826
摘要： The invention provides a droplet actuator comprising a base substrate and a top substrate separated to form a gap, wherein: (a) the base substrate comprises electrodes configured for conducting droplet operations in the gap; and (b) the top substrate comprises a first portion coupled to second portion, where the second portion comprises one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.
摘要翻译： 本发明提供了一种液滴致动器，其包括分离的基底基板和顶部基板以形成间隙，其中：（a）基底基板包括构造成用于在间隙中进行液滴操作的电极; 和（b）顶部衬底包括联接到第二部分的第一部分，其中第二部分包括建立从液滴致动器的外部延伸到间隙中的流体路径的一个或多个开口。 本发明还提供了制造液滴致动器的相关方法，使用液滴致动器的方法以及装载液滴致动器的方法。
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公开(公告)号：US20100221713A1
公开(公告)日：2010-09-02
申请号：US12546115
申请日：2009-08-24
申请人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
发明人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
IPC分类号： C12Q1/68
CPC分类号： B01L3/502792 , B01L3/502753 , B01L3/502761 , B01L7/525 , B01L2200/10 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/6848 , C12Q2565/629
摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.
摘要翻译： 本发明涉及在液滴致动器上通过液体填充液减少或消除污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，液滴致动器用于使用聚合酶链反应（PCR）技术进行遗传分析。 本发明解决了对提供样品靶的最佳放大和检测的在液滴致动器上进行PCR的改进方法的需要。
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公开(公告)号：US20100190263A1
公开(公告)日：2010-07-29
申请号：US12692954
申请日：2010-01-25
CPC分类号： C07K1/26 , B01F13/0076 , B01F13/0084 , B01F13/0086 , B01L3/502723 , B01L3/502738 , B01L3/502792 , B01L2200/0673 , B01L2300/0867 , B01L2300/089 , B01L2400/0406 , B01L2400/0427 , B01L2400/0688 , G01N2001/2244 , G01N2035/1034 , Y10T436/25
摘要： The present invention is directed to a droplet actuator and methods of making and using the droplet actuator including one or more substrates configured to form a droplet operations gap and including a physical or chemical feature that may be provided at a predetermined locus within or exposed to the droplet operations gap and configured to retain a bubble in position within the droplet operations gap.
摘要翻译： 本发明涉及液滴致动器以及制造和使用液滴致动器的方法，所述液滴致动器包括一个或多个被配置成形成液滴操作间隙的基板，并且包括物理或化学特征，该物理或化学特征可以设置在或 液滴操作间隙并且被配置为将气泡保持在液滴操作间隙内的适当位置。
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17.发明申请公开(公告)号：US20130217113A1
公开(公告)日：2013-08-22
申请号：US13809762
申请日：2011-07-12
申请人： Vijay Srinivasan , William Craig Bauer , Gregory F. Smith , Ryan A. Sturmer , Zhishan Hua , Allen E. Eckhardt
发明人： Vijay Srinivasan , William Craig Bauer , Gregory F. Smith , Ryan A. Sturmer , Zhishan Hua , Allen E. Eckhardt
IPC分类号： C12M1/42
CPC分类号： C12M1/42 , B01L3/502792 , B01L2200/0647 , B01L2300/0672 , B01L2400/0427 , B01L2400/0439 , B01L2400/088 , C12M47/06 , C12N1/066 , C12N13/00
摘要： The invention relates to a droplet actuator for conducting droplet operations. The actuator includes a bottom substrate and a top substrate separated from the bottom substrate to form a gap. An arrangement of droplet operations electrodes may be located on a surface of the bottom substrate and/or top substrate. Optionally, a sample reservoir may hold a quantity of a sample fluid containing cells. A disruption device which can take various forms is used to lyse the cells in the sample or in a sample droplet to thereby conduct operations on samples having lysed cells therein.
摘要翻译： 本发明涉及一种用于进行液滴操作的液滴致动器。 致动器包括底部基板和与底部基板分离以形成间隙的顶部基板。 液滴操作电极的布置可以位于底部基板和/或顶部基板的表面上。 任选地，样品储存器可以容纳一定量的含有细胞的样品流体。 可以采用各种形式的破坏装置来裂解样品或样品液滴中的细胞，从而对其中具有裂解细胞的样品进行操作。
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公开(公告)号：US08088578B2
公开(公告)日：2012-01-03
申请号：US12546253
申请日：2009-08-24
申请人： Zhishan Hua , Michael G. Pollack
发明人： Zhishan Hua , Michael G. Pollack
CPC分类号： B01L3/502792 , B01L3/502753 , B01L3/502761 , B01L7/525 , B01L2200/10 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/6848 , C12Q2565/629
摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, a method of detecting an analyte is provided and includes providing in a detection window a droplet including a signal-producing substance indicative of the presence and/or quantity of an analyte and one or more magnetically responsive beads which may interfere with signal produced by the signal producing substance. The method further includes using a magnetic field for magnetically removing the magnetically responsive beads from the detection window and/or magnetically restricting the magnetically responsive beads from entering the detection window while transporting and/or retaining the droplet in the detection window. The method additionally includes detecting a signal produced by the signal-producing substance.
摘要翻译： 本发明涉及在液滴致动器上通过液体填充液减少或消除污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，提供检测分析物的方法，并且包括在检测窗口中提供包含指示分析物的存在和/或量的信号产生物质的液滴和一个或多个可能干扰信号的磁响应珠子 由信号产生物质产生。 该方法还包括使用磁场来从检测窗口磁性地去除磁响应珠子和/或磁力限制磁响应珠子进入检测窗口，同时在检测窗口中传送和/或保持液滴。 该方法还包括检测由信号产生物质产生的信号。
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公开(公告)号：US08551599B2
公开(公告)日：2013-10-08
申请号：US12551071
申请日：2009-08-31
申请人： Zhishan Hua , Erdogan Gulari , Mark A. Burns , Rohit Pal , Onnop Srivannavit
发明人： Zhishan Hua , Erdogan Gulari , Mark A. Burns , Rohit Pal , Onnop Srivannavit
CPC分类号： F16K99/0032 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L2300/0819 , B01L2400/0481 , B01L2400/0655 , B01L2400/0677 , F15B15/10 , F16K99/0044 , Y10T137/0391 , Y10T137/1624 , Y10T137/1812 , Y10T137/2142 , Y10T137/218 , Y10T137/2191 , Y10T137/2196 , Y10T137/2202 , Y10T137/2218 , Y10T428/23 , Y10T428/234 , Y10T428/24322 , Y10T428/24331 , Y10T428/24612
摘要： A microactuator may be configured by activating a source of electromagnetic radiation to heat and melt a selected set of phase-change plugs embedded in a substrate of the microactuator, pressurizing a common pressure chamber adjacent to each of the plugs to deform the melted plugs, and deactivating the source of electromagnetic radiation to cool and solidify the melted plugs.
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公开(公告)号：US20130017544A1
公开(公告)日：2013-01-17
申请号：US13546817
申请日：2012-07-11
申请人： Allen E. Eckhardt , Zhishan Hua
发明人： Allen E. Eckhardt , Zhishan Hua
CPC分类号： G01N25/04 , B01F13/0071 , B01F13/0076 , B01L3/502792 , B01L7/525 , B01L2200/0668 , B01L2300/0867 , B01L2400/0427 , B01L2400/043 , G01N21/6428 , G01N2021/6439
摘要： An integrated droplet actuator device and methods are provided for performing PCR amplification and high-resolution melting (HRM) analysis on a single droplet actuator. HRM analysis can be used in combination with PCR amplification for detection of sequence variations (e.g., single-nucleotide polymorphisms, nucleotide-repeat polymorphisms, mutation scanning and assessment of DNA methylation) within one or more genes of interest. The PCR amplicons can be fluorescently labeled during amplification using a saturating DNA intercalating fluorescent dye, a 5′-labeled primer, or labeled probes. Also provided are a droplet actuator device and methods for sample preparation using the droplet actuator and detection of sequence variations on the same droplet actuator.
摘要翻译： 提供了一种集成液滴致动器装置和方法，用于在单个液滴致动器上执行PCR扩增和高分辨率熔解（HRM）分析。 HRM分析可以与PCR扩增结合使用，用于检测一个或多个感兴趣的基因中的序列变异（例如，单核苷酸多态性，核苷酸重复多态性，突变扫描和DNA甲基化的评估）。 使用饱和DNA插入荧光染料，5'-标记的引物或标记的探针，PCR扩增子可以在扩增过程中进行荧光标记。 还提供了液滴致动器装置和使用液滴致动器进行样品制备的方法以及在相同液滴致动器上检测序列变化的方法。
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