摘要:
The present invention relates to a processing method for processing a substrate, and comprises a step of coating a coating solution on a surface of the substrate while relatively moving a coating solution discharge nozzle and the substrate and discharging the coating solution from the nozzle onto the substrate. Thereafter, the substrate is exposed to a solvent atmosphere of the coating solution or the pressure is temporarily applied thereto in a container. Thereafter, the pressure inside the container in which the substrate is housed is reduced to dry the coating solution on the substrate. According to the present invention, it is possible to narrow the so-called edge cutting width, which is at a periphery part of the substrate and is not commercialized, and to maintain an in-plane uniformity of the coating film.
摘要:
The present invention provides novel polypeptides and polynucleotides useful as biotechnological tools, specifically identified in a coryneform bacterium Corynebacterium glutamicum ssp. lactofermentum and methods of producing substances in organisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.
摘要:
The present invention provides a process for preparing a transplantable liver tissue, cell colonies suitable for the process, and a process for preparing them. At least about 70 % of the cells in the small hepatocyte-rich colonies of the present invention are small hepatocytes. In particular, each colony comprises about 10 to 30 cells and the number of the small hepatocytes is at least about 70 % based on the total number of the cells in the colony. A process for preparing such small hepatocyte-rich colonies is also disclosed. The present invention also discloses a process for inducing the maturation of the small hepatocyte-rich colonies into a liver tissue and a method of estimating the effect of a drug, particularly the effect thereof connected with normal liver function in vitro, using the cell colonies enriched with the small hepatocytes which have been induced to be matured.
摘要:
The present invention provides polypeptides and polynucleotides involved in the stress response to environmental changes in Methylophilus methylotrophus and methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.
摘要:
The present invention provides polypeptides and polynucleotides involved in the stress response to environmental changes in Methylophilus methylotrophus and methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.
摘要:
An Escherichia coli mutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed with a chromosomal gene library of Bacillus methanolicus, and a transformant strain which can grow on a minimal medium is selected. Recombinant DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase is obtained from the transformant.
摘要:
A coating solution is sprayed on a rotating wafer held horizontally from a nozzle provided above the wafer while the nozzle is travelling over the wafer from a wafer center to a wafer outer area, thus spirally spraying the coating solution on the wafer. The nozzle stops when the coating solution has reached the wafer outer area and the coating solution is sprayed in circle on the wafer outer area while the wafer is rotating. A coating solution including a component of a coating film and a solvent may be sprayed on a first area to be coated of the wafer and the coating solution and a solvent for the coating film may be sprayed on a second edge area located outside the first area of the wafer.
摘要:
A plasmid which is able to be isolated from Corynebacterium thermoaminogenes, and which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 4 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 4. and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.
摘要翻译:能够从嗜热棒杆菌分离的质粒,其包含编码具有SEQ ID NO:4所示的氨基酸序列的Rep蛋白的基因或与氨基酸同源性为90%以上的氨基酸序列 SEQ ID NO:4所示的序列,其大小为约4.4kb或约6kb,或其衍生物。
摘要:
A mounting apparatus includes: a heater head for bonding a liquid crystal display and a flexible printed circuit board by thermocompression; a cylinder as a heater head driving means for driving heater head to compress the liquid crystal display and flexible printed circuit board by a prescribed load; and a control mechanism as a stretch amount controlling means for adjusting a load change per unit of time after the heater head starts compressing the flexible printed circuit board by the cylinder as well as a time at which a required load is attained to control the stretch amount of the flexible printed circuit board by thermocompression. Preferably, the mounting apparatus is provided with a mechanism which performs measurement after preliminary bonding and which performs regular bonding while controlling based on the obtained information.
摘要:
The present invention provides dihydropicolinate synthase and dihydrodipicolinate reductase enzymes from Bacillus methanolicus, polynucleotides encoding the enzymes, and methods of producing L-lysinse in microorganisms expressing the enzymes.