公开(公告)号：US09327212B2

公开(公告)日：2016-05-03

申请号：US13497896

申请日：2010-09-23

申请人： Torbjorn Blank ,  Enrique Carredano ,  Karl Ekstrom ,  Jan-Erik Lynga ,  Roger Nordberg ,  Mike Peters ,  Gustav Rodrigo ,  Henrik Sandegren ,  Lisa Stack

发明人： Torbjorn Blank ,  Enrique Carredano ,  Karl Ekstrom ,  Jan-Erik Lynga ,  Roger Nordberg ,  Mike Peters ,  Gustav Rodrigo ,  Henrik Sandegren ,  Lisa Stack

IPC分类号： B01D15/16 ,  G01N30/34 ,  G05D11/13 ,  B01F15/00

CPC分类号： B01D15/166 ,  B01F15/0022 ,  B01F15/00227 ,  G01N30/34 ,  G01N2030/347 ,  G05D11/132

摘要： A method for preparing a liquid flow having pre-defined characteristics by mixing liquid flows of at least two different component stock solutions with each other comprises the steps of determining a selected property value for one or more of the stock solutions by sensing, in a flow of each stock solution separately, at least one characteristic related to the property value for the stock solution, and based on the determined property value or values, mixing the stock solution flows in mixing ratios giving the desired mixed liquid flow. A system for carrying out the method comprises sensor means and a control unit, wherein the control unit is arranged to evaluate the characteristics sensed by the sensor means and provide relative ratios of stock solutions required to obtain a mixed liquid flow having the pre-defined characteristics.

摘要翻译： 通过混合至少两种不同组分原料溶液的液体流相互混合制备具有预定特性的液体流的方法包括以下步骤：通过在流体中感测来确定一种或多种储备溶液的选定的特性值 分别存储与储备溶液的特性值相关的至少一个特征，并且基于所确定的特性值或值，以提供所需混合液体流的混合比混合储备液流。 用于执行该方法的系统包括传感器装置和控制单元，其中控制单元布置成评估由传感器装置感测到的特性，并提供获得具有预定特性的混合液流所需的储备溶液的相对比例 。

公开(公告)号：US20160077065A1

公开(公告)日：2016-03-17

申请号：US14953856

申请日：2015-11-30

申请人： Kihachiro Okura

发明人： Kihachiro Okura

IPC分类号： G01N30/34

CPC分类号： G01N30/34 ,  B01D15/166 ,  G01N30/8631 ,  G01N2030/027 ,  G01N2030/347

摘要： Provided that elapsed time from the start of the flow-in of an eluent into a column is t0 and the mobility Rfc(t/t0) of a component c in a sample is represented by a function of elapsed time t from the start of the flow-in of a sample into the column, elution time trc from the flow-in of the sample into the column to the flow-out of the component c from the column is calculated by using Equation (1). In doing so, the mobility Rfc(t/t0) in Equation (1) is represented by Equation (2). ∫ 0 t r c  R f c  ( t t 0 )    ( t t 0 ) = 1 ( 1 ) R f c  ( t t 0 ) = 1 -  - a  ( t t 0 ) + b ( 2 )

摘要翻译： 假设从洗脱液流入列开始的经过时间为t 0，并且样品中成分c的迁移率Rfc（t / t0）由从开始时的经过时间t的函数表示 将样品流入柱中，通过使用等式（1）计算从样品进入柱的流入到从柱中流出的组分c的洗脱时间trc。 在这样做时，式（1）中的迁移率Rfc（t / t0）由等式（2）表示。 （t t 0）= 1（1）R f c（t t 0）= 1 -  - a（t t 0）+ b（2）∫0 t r c（t t 0）

公开(公告)号：US20160069845A1

公开(公告)日：2016-03-10

申请号：US14782476

申请日：2014-05-15

申请人： WATER TECHNOLOGIES CORPORATION

发明人： Michael O. Fogwill ,  Joseph D. Michienzi ,  James P. Murphy

IPC分类号： G01N30/30 ,  G01N30/16 ,  G01N30/32

CPC分类号： G01N30/30 ,  B01D15/161 ,  B01D15/40 ,  G01N30/16 ,  G01N30/32 ,  G01N30/34 ,  G01N30/7213 ,  G01N30/84 ,  G01N2030/3038

摘要： A chromatography system includes a separation column that separates a sample carried by a compressible mobile phase flow into analytes and a splitter in fluidic communication with the separation column to receive and divide the compressible mobile phase flow into first and second mobile phase streams in accordance with a split ratio. A thermally modulated variable restrictor is coupled between the splitter and a detector. The restrictor receives the first mobile phase stream from the splitter and has a temperature element in thermal communication with the first mobile phase stream to exchange heat therewith. A controller, in communication with the restrictor, dynamically adjusts a temperature setting of the temperature element of the restrictor to adjust the heat exchange between the thermally modulated variable restrictor and the first mobile phase stream in order to keep the split ratio constant throughout a chromatographic run.

摘要翻译： 色谱系统包括分离柱，其将由可压缩流动相流携带的样品分离成分析物，以及与分离塔流体连通的分离器，以将可压缩流动相流接收并分成第一和第二流动相流，根据 分流比。 热调制变量限制器耦合在分路器和检测器之间。 限流器从分流器接收第一流动相流，并且具有与第一流动相流热连通的温度元件以与其进行热量交换。 与节流器连通的控制器动态调节限流器的温度元件的温度设置，以调节热调节的可变节流器和第一流动相流之间的热交换，以便在整个色谱运行期间保持分流比恒定 。

公开(公告)号：US20160003797A1

公开(公告)日：2016-01-07

申请号：US14765649

申请日：2014-02-28

申请人： ST. JUDE CHILDREN'S RESEARCH HOSPITAL

发明人： Amar K. Pani ,  Richard Jay Smeyne

IPC分类号： G01N33/487 ,  G01N27/42

CPC分类号： G01N33/48707 ,  A61K31/52 ,  B01D15/327 ,  G01N27/423 ,  G01N30/34 ,  G01N30/64 ,  G01N2030/8827 ,  Y10T436/141111 ,  Y10T436/142222 ,  Y10T436/147777 ,  Y10T436/173845 ,  Y10T436/25375

摘要： This invention relates is a label-free, enzyme-free, aptamer-free method for simultaneously measuring adenosine, and its intracellular metabolites, e.g., AMP, ADP and ATP, using high pressure liquid chromatography coupled to electrochemical detector (HPLC-ECD).

摘要翻译： 本发明涉及使用连接到电化学检测器（HPLC-ECD）的高压液相色谱法同时测量腺苷及其细胞内代谢物例如AMP，ADP和ATP的无标记，无酶，不适合的方法。

公开(公告)号：US20150346168A1

公开(公告)日：2015-12-03

申请号：US14822527

申请日：2015-08-10

申请人： WATERS TECHNOLOGIES CORPORATION

发明人： Steven J. Ciavarini ,  Stanley P. Pensak, JR. ,  Jeffrey W. Finch ,  Keith Fadgen ,  Hongji Liu

IPC分类号： G01N30/34 ,  G01N30/32

CPC分类号： G01N30/34 ,  G01N30/32 ,  G01N2030/326 ,  G05D11/132

摘要： A solvent delivery subsystem for a chromatography device performs relatively low pressure, high flow mixing of solvents to form a gradient and subsequent high pressure, low flow delivery of the gradient to the separation column. The mixing of the gradient is independent and does not interfere with the gradient delivery. To form the gradient, the outputs of an aqueous pump and an organic pump are mixed to fill a storage capillary while a downstream point from the storage capillary is vented to atmosphere. After gradient formation, the vent to atmosphere is closed, the solvent delivery system rises to high pressure, and only the aqueous pump runs for gradient delivery. To maintain integrity of the fluid stream, the solvent delivery system uses feed forward compensation and controls at least one parameter selected from the group consisting of pressure and flow in the conduit means to follow a gradual ramp.

公开(公告)号：US20150336026A1

公开(公告)日：2015-11-26

申请号：US14696572

申请日：2015-04-27

申请人： Waters Technologies Corporation

发明人： Neal B. Almeida ,  Christopher Seith ,  Michael R. Jackson ,  John Angelosanto ,  Joseph A. Jarrell

IPC分类号： B01D15/18 ,  G01N30/26

CPC分类号： B01D15/18 ,  G01N30/26 ,  G01N30/34 ,  Y10T137/0424 ,  Y10T137/4259

摘要： Described is a method for purging a fluid channel is a low pressure gradient formation liquid flow system. Control of the fluid channels for multiple solvents allows for one or more static volumes of solvents not intended for use in an isocratic flow to be purged from their fluid channels to avoid contamination of the isocratic solvent. Advantageously, the method avoids the need to modify equipment or to reconfigure a pumping system so that the inlet is directly coupled to a single solvent source. Thus there is no need to bypass existing valves and liquid coupling components where solvents are combined during conventional gradient operation.

摘要翻译： 描述了一种清洗流体通道的方法是低压梯度形成液体流动系统。 用于多种溶剂的流体通道的控制允许一个或多个静态体积的溶剂不用于从其流体通道中清除等度流，以避免污染等度溶剂。 有利地，该方法避免了修改设备或重新配置泵送系统的需要，使得入口直接耦合到单个溶剂源。 因此，在常规梯度操作期间，不需要绕过现有的阀和液体耦合部件，其中溶剂被组合。

公开(公告)号：US20150316516A1

公开(公告)日：2015-11-05

申请号：US14648065

申请日：2013-10-28

申请人： AGILENT TECHNOLOGIES, INC.

发明人： Robert William ALBRECHT, JR.

IPC分类号： G01N30/20

CPC分类号： G01N30/20 ,  B01L3/502784 ,  G01N30/34 ,  G01N30/38 ,  G01N2030/027 ,  G01N2030/207 ,  G01N2030/347

摘要： In a liquid chromatography system, a sample is injected into a column by flowing a solvent mixture from a mixer into the column along a solvent mixture flow path; and injecting a sample into the solvent mixture flow path downstream of the mixer. In another liquid chromatography system, a sample is injected into a column by loading an isolator fluid into a sample loop, loading a sample into the sample loop, and flowing the sample into the column as a plug in front of the isolator fluid.

摘要翻译： 在液相色谱系统中，通过将溶剂混合物从混合器沿着溶剂混合物流动路径流入柱中，将样品注入柱中; 并将样品注入混合器下游的溶剂混合物流动路径。 在另一种液相色谱系统中，将样品通过将隔离器流体加载到样品环中，将样品装入样品环中并将样品作为隔离器流体前面的塞子流入柱中而注入柱中。

公开(公告)号：US20150276688A1

公开(公告)日：2015-10-01

申请号：US14668000

申请日：2015-03-25

申请人： SHIMADZU CORPORATION

发明人： Kengo Aoki ,  Keisuke Ogawa

IPC分类号： G01N30/20 ,  G01N30/86 ,  B01D15/08

CPC分类号： G01N30/34 ,  B01D15/08 ,  B01D15/16 ,  B01D15/166 ,  B01D15/18 ,  G01N30/20 ,  G01N30/8658 ,  G01N30/8665 ,  G01N30/8675 ,  G01N2030/347 ,  G01N2030/383 ,  G01N2030/385

摘要： A liquid chromatograph has a mobile phase delivery section including, between mobile phase channels connected respectively to a plurality of mobile phase containers and a delivery pump, channel switching valves for selecting one of the mobile phase channels. This liquid chromatograph whose channel switching valves have a hierarchical structure including at least two stages includes a valve connection state setting section that is configured to set the connection state between the channel switching valves and the connection state between the channel switching valves and the mobile phase channels, and further includes a per channel total delivery amount calculation section that is configured to calculate the total delivery amount of mobile phase per mobile phase channel based on a connection state according to the valve connection state setting section, the delivery amount per unit time by the delivery pump, and the operation time of the delivery pump.

摘要翻译： 液相色谱仪具有流动相递送部分，其包括分别连接到多个流动相容器的流动相通道和输送泵之间，用于选择流动相通道之一的通道切换阀。 这种液相色谱仪的通道切换阀具有包括至少两级的分层结构，包括阀连接状态设定部，其被配置为设置通道切换阀之间的连接状态和通道切换阀与流动相通道之间的连接状态 并且还包括每通道总量递送量计算部分，其被配置为基于根据阀连接状态设置部分的连接状态来计算每个移动通道的流动相的总输送量，每单位时间的输送量由 输送泵，以及输送泵的运行时间。

公开(公告)号：US09128071B2

公开(公告)日：2015-09-08

申请号：US13704247

申请日：2011-05-18

申请人： Nobuhiro Tsukada ,  Yoshihiro Nagaoka ,  Hironori Kaji ,  Daisuke Akieda

发明人： Nobuhiro Tsukada ,  Yoshihiro Nagaoka ,  Hironori Kaji ,  Daisuke Akieda

IPC分类号： G01N30/02 ,  B01F5/06 ,  G01N30/34

CPC分类号： G01N30/02 ,  B01F5/0646 ,  G01N30/34

摘要： A liquid mixing device that decreases a concentration non-uniformity in the flow direction of a mobile phase and a liquid chromatograph that uses the liquid mixing device are provided. The liquid mixing device is configured to include a flow channel unit that is made from an introduction channel, a branch portion that is positioned in a downstream of the introduction channel, multiple branched flow channels that branch from the branch portion, a junction portion in which the multiple branched flow channels join together, and a discharge channel of the downstream of the junction portion. The multiple branched flow channels are different in terms of any one of, or several of width, depth, and length that are associated with an external shape, and a structure filling the inside of the flow channel and thus the times for the liquid to pass through the branched flow channels are different from each other.

摘要翻译： 提供了降低流动相的流动方向的浓度不均匀性和使用液体混合装置的液相色谱仪的液体混合装置。 液体混合装置被构造成包括由引入通道制成的流动通道单元，位于引入通道下游的分支部分，从分支部分分支的多个分支流动通道，其中连接部分 多个分支流动通道连接在一起，以及连接部分下游的排出通道。 多个分支流动通道在与外部形状相关联的宽度，深度和长度中的任何一个或几个方面是不同的，以及填充流动通道内部的结构，因此液体通过的时间 通过分支流动通道彼此不同。

公开(公告)号：US09103814B2

公开(公告)日：2015-08-11

申请号：US12280585

申请日：2007-03-16

申请人： Steven J. Ciavarini ,  Stanley P. Pensak, Jr. ,  Jeffrey W. Finch ,  Keith Fadgen ,  Hongji Liu

发明人： Steven J. Ciavarini ,  Stanley P. Pensak, Jr. ,  Jeffrey W. Finch ,  Keith Fadgen ,  Hongji Liu

IPC分类号： G01N30/34

CPC分类号： G01N30/34 ,  G01N30/32 ,  G01N2030/326 ,  G05D11/132

摘要： A solvent delivery subsystem for a chromatography device performs relatively low pressure, high flow mixing of solvents to form a gradient and subsequent high pressure, low flow delivery of the gradient to the separation column. The mixing of the gradient is independent and does not interfere with the gradient delivery. To form the gradient, the outputs of an aqueous pump and an organic pump are mixed to fill a storage capillary while a downstream point from the storage capillary is vented to atmosphere. After gradient formation, the vent to atmosphere is closed, the solvent delivery system rises to high pressure, and only the aqueous pump runs for gradient delivery. To maintain integrity of the fluid stream, the solvent delivery system uses feed forward compensation and controls at least one parameter selected from the group consisting of pressure and flow in the conduit means to follow a gradual ramp.

摘要翻译： 用于色谱装置的溶剂递送子系统执行溶剂的相对低压，高流动混合以形成梯度并随后将高梯度梯度递送到分离塔。 梯度的混合是独立的，并且不干扰梯度递送。 为了形成梯度，将水性泵和有机泵的输出混合以填充储存毛细管，同时从储存毛细管的下游点排放到大气中。 梯度形成后，关闭空气，溶剂输送系统升高至高压，只有水泵运行梯度输送。 为了保持流体流的完整性，溶剂输送系统使用前馈补偿并且控制选自管道装置中的压力和流量的至少一个参数以跟随逐渐斜坡。