公开(公告)号：US20150011430A1

公开(公告)日：2015-01-08

申请号：US14493272

申请日：2014-09-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Serge Saxonov

IPC: C12Q1/68

CPC classification number: C12N15/1065 ,  C12N15/10 ,  C12N15/1075 ,  C12Q1/6876 ,  C12Q2525/191 ,  C12Q2563/179

Abstract: Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.

公开(公告)号：US20140378348A1

公开(公告)日：2014-12-25

申请号：US14312488

申请日：2014-06-23

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Anthony J. Makarewicz, JR. ,  Luc Bousse ,  Stefano Schiaffino

IPC: B01L3/00 ,  C12N15/10

CPC classification number: B01L3/502784 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/50853 ,  B01L7/52 ,  B01L2200/027 ,  B01L2300/04 ,  B01L2300/041 ,  B01L2300/044 ,  B01L2300/046 ,  B01L2300/0672 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0655 ,  B01L2400/0677 ,  B01L2400/084 ,  C12N15/1075 ,  C12Q1/6806 ,  Y10T436/2575

Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In some embodiments, the system may include a well and a channel component attached to the well. The channel component may include (a) a body, (b) an input tube (a “fluid pickup”) projecting from a bottom surface of the body and having an open bottom end disposed in the input well, (c) a microchannel, and (d) a passage extending through the input tube and the body and connecting the well to the microchannel. The system may be configured to receive a sample-containing fluid in the well and retain the sample-containing fluid below a top end of the passage, until a pressure differential is created that drives at least a portion of the sample-containing fluid from the well via the passage and through the microchannel.

Abstract translation: 微流体系统，包括用于处理流体的方法和装置，例如通过液滴产生。 在一些实施例中，系统可以包括井和连接到井的通道部件。 通道部件可以包括（a）主体，（b）从主体的底表面突出并具有设置在输入孔中的敞开的底端的输入管（“流体拾取器”），（c）微通道， 和（d）延伸穿过输入管和主体并将井连接到微通道的通道。 该系统可以被配置为在井中接收含样品的流体并将含样品的流体保持在通道的顶端下方，直到形成压力差，从而将含有样品的流体的至少一部分从 通过通道和通过微通道。

公开(公告)号：US20140327349A1

公开(公告)日：2014-11-06

申请号：US14266501

申请日：2014-04-30

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Jeffry M. Ceremony ,  Tom McCollum

IPC: B01L7/00

CPC classification number: B01L7/52 ,  B01L2300/041 ,  B01L2300/046 ,  B01L2300/0829 ,  B01L2300/1827

Abstract: A drawer apparatus having a drawer chassis and a drawer adapted to move relative to the drawer chassis from a closed position to an open position. The drawer has first and second lateral sides. A lid assembly is moveable from an upward position to a downward position relative to the drawer chassis. The lid assembly has first and second roller members. A first lever arm is rotatable about a first pivot on the first lateral side. The first lever arm has a first surface for moveably engaging with the first roller member. A second lever arm is rotatable about a second pivot on the second lateral side. The second lever arm has a second surface for moveably engaging with the second roller. A handle connects the first and second lever arms. The handle is adapted to move the first and second lever arms respectively about the first and second pivots to move the lid assembly from the downward position to the upward position.

Abstract translation: 一种抽屉设备，其具有抽屉底盘和适于相对于抽屉底盘从关闭位置移动到打开位置的抽屉。 抽屉有第一和第二侧面。 盖组件可相对于抽屉底座从向上位置移动到向下位置。 盖组件具有第一和第二辊构件。 第一杠杆臂可围绕第一侧面的第一枢轴转动。 第一杠杆臂具有用于与第一辊构件可移动地接合的第一表面。 第二杠杆臂可绕第二横向侧上的第二枢轴旋转。 第二杠杆臂具有用于与第二辊可动地接合的第二表面。 手柄连接第一和第二杠杆臂。 手柄适于分别围绕第一和第二枢轴移动第一和第二杠杆臂，以将盖组件从向下位置移动到向上位置。

公开(公告)号：US20140293412A1

公开(公告)日：2014-10-02

申请号：US14262559

申请日：2014-04-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael Griffin ,  Daniel Yue Man Chu ,  Tom McCollum

IPC: G02B21/06

CPC classification number: G02B21/06 ,  G02B21/125 ,  G02B21/16 ,  G02B21/24 ,  G02B21/365

Abstract: A system, apparatus and method for using modular microscopes is disclosed. Connecting the housings of the individual microscope modules provide the structural framework of the modular microscope. Furthermore, the modular microscope can include specialized software, the distribution and use of which can be controlled using security keys or identifiers stored on one or more of the microscope modules. The security keys and identifiers can be based on calibration data associated with the physical, electrical, or optical properties of one of more of the modules. The illumination modules disclosed provide for selectable wavelengths and controllable levels of output illumination for both bright field and dark field illumination.

Abstract translation: 公开了一种使用模块显微镜的系统，装置和方法。 连接各个显微镜模块的外壳提供了模块化显微镜的结构框架。 此外，模块化显微镜可以包括专门的软件，可以使用存储在一个或多个显微镜模块上的安全密钥或标识符来控制其分布和使用。 安全密钥和标识符可以基于与多个模块之一的物理，电或光学特性相关联的校准数据。 所公开的照明模块为亮场和暗场照明提供了可选择的波长和输出照明的可控电平。

公开(公告)号：US20140272995A1

公开(公告)日：2014-09-18

申请号：US14216006

申请日：2014-03-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Erin R. Chia ,  Amy L. Hiddessen ,  Benjamin J. Hindson ,  Adam Lowe ,  Chunxiao Han

IPC: C12Q1/68 ,  C07F7/18

CPC classification number: C07F7/1804 ,  C08G77/46 ,  C08G77/50 ,  C08L83/12 ,  C08L83/14 ,  G01N33/5436

Abstract: System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.

Abstract translation: 用于制备和使用包括硅油和硅氧烷表面活性剂的乳液的方法和组合物的系统。 乳液可以包括设置在包括硅油和硅氧烷表面活性剂的连续相中的水性液滴。 水滴可以含有任选地部分占据的分析物和/或发光（例如光致发光）报告物。 分析物的测定可以用液滴进行。 在一些情况下，可以从液滴中检测信号，并且可以基于该信号来确定分析物的特征，例如分析物水平或活性。

公开(公告)号：US20140260970A1

公开(公告)日：2014-09-18

申请号：US14209974

申请日：2014-03-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Nathan Wrench ,  William Mainwaring-Burton

IPC: B01D19/00

CPC classification number: B01D19/0031

Abstract: Disclosed herein is an apparatus for debubbling or degassing a liquid. The apparatus includes a tube with a hollow lumen and a gas-permeable, liquid-impermeable wall surrounding the lumen. Also disclosed is a method for performing the debubbling or degassing, wherein the liquid is passed through the tube and gas is allowed to escape through the wall of the tube. The application additionally discloses a system for debubbling or degassing a liquid, wherein a pump is connected to the upstream end of the tube and a device to impede the flow of liquid out the tube is connected to the downstream end.

Abstract translation: 本文公开了一种用于对液体进行脱泡或脱气的装置。 该装置包括具有中空腔的管和围绕内腔的透气的液体不可渗透的壁。 还公开了一种用于进行脱泡或脱气的方法，其中液体通过管，并且允许气体通过管的壁逸出。 该应用还公开了一种用于对液体进行脱泡或脱气的系统，其中泵连接到管的上游端，并且阻止液体流出管的装置连接到下游端。

公开(公告)号：US20140228239A1

公开(公告)日：2014-08-14

申请号：US14173562

申请日：2014-02-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam M. McCoy ,  Svilen Tzonev ,  Frank Hsiung

IPC: G01N33/566

CPC classification number: C12Q1/00 ,  G01N33/542 ,  G01N33/581

Abstract: The present invention provides methods of detecting a target molecule in a sample comprising incubating the sample with two or more detectably labeled probes, partitioning the sample into multiple partitions, and detecting the presence of the two or more probes in the same partition.

Abstract translation: 本发明提供了检测样品中靶分子的方法，包括用两个或多个可检测标记的探针孵育样品，将样品分成多个分区，以及检测两个或更多个探针在同一分区中的存在。

公开(公告)号：US20140220577A1

公开(公告)日：2014-08-07

申请号：US13924353

申请日：2013-06-21

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： YAN WANG ,  PETER VANDER HORN ,  LEI XI

IPC: C12Q1/68

CPC classification number: C12N15/10 ,  C07K14/195 ,  C07K2319/00 ,  C07K2319/80 ,  C12N9/1252 ,  C12N15/62 ,  C12P19/34 ,  C12Q1/686 ,  C12Y207/07007

Abstract: This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.

Abstract translation: 本发明提供使用改进的核酸聚合酶的产生来测序和进行聚合酶反应的方法。 改进是以提高聚合酶的持续性的方式将序列非特异性核酸结合结构域与酶的融合。

公开(公告)号：US20140157859A1

公开(公告)日：2014-06-12

申请号：US14100397

申请日：2013-12-09

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam Darmstadt ,  Michael Waite ,  Theresa Forni

IPC: G01N33/49

CPC classification number: G01N35/00693 ,  G01N35/0095 ,  G01N2030/047 ,  G01N2035/00702

Abstract: An improved calibration process for a medical testing machine. The machine automatically recognizes that a package of calibration material has been inserted into it, and performs a calibration sequence to ascertain a calibration parameter to be used in performing future tests with the medical testing machine. The calibration package may include machine-readable indicators that the package is to be used for calibration, and of a calibration setpoint of a calibration material in the package. A calibration material may be stored in a lyophilized state in the package, and the medical testing machine may automatically reconstitute the lyophilized material.

Abstract translation: 用于医疗测试机器的改进的校准过程。 机器自动识别出已经插入校准材料包，并执行校准顺序，以确定用于在医学测试机器中进行将来测试中使用的校准参数。 校准包可以包括用于校准的包装的机器可读指示器以及包装中的校准材料的校准设定点。 校准材料可以以冻干状态存储在包装中，并且医学检测机器可以自动重构冻干的材料。

公开(公告)号：US20140057293A1

公开(公告)日：2014-02-27

申请号：US13973596

申请日：2013-08-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Thomas R. Berkelman ,  Lisandra Martin ,  Rhiannon Jones ,  John Walker, II

IPC: G01N33/543 ,  C08F220/60

CPC classification number: G01N27/44747 ,  B01D15/3823 ,  C07D255/02 ,  C07F3/06 ,  C07F13/005 ,  C08F220/56 ,  C08F220/60 ,  G01N33/54306 ,  Y10T436/143333 ,  Y10T436/163333

Abstract: Conjugates of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes with a variety of conjugating members are used in the formation of dinuclear metal complexes which bind to phosphate esters. By virtue of their conjugated forms, the complexes are incorporated into chromatographic media, affinity binding reagents, and dyes, which make the complexes useful in a wide range of assays, separations, and purifications. In addition, dinuclear metal complexes of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes that are not so conjugated are used in the detection of phosphate esters of biological species by either MALDI-TOF mass spectrometry or by dye displacement.

Abstract translation: 将1,3-双（1,4,7-三氮杂环壬-1-烯）-2-羟基丙烷与多种共轭成员的共轭物用于形成与磷酸酯结合的双核金属络​​合物。 由于它们的共轭形式，复合物被并入到色谱介质，亲和力结合试剂和染料中，这使复合物可用于广泛的测定，分离和纯化。 此外，不结合的1,3-双（1,4,7-三氮杂环壬烷-1-基）-2-羟基丙烷的双核金属络​​合物用于通过MALDI-TOF检测生物物种的磷酸酯 质谱或染料置换。