公开(公告)号：US11981952B2

公开(公告)日：2024-05-14

申请号：US16915454

申请日：2020-06-29

Applicant: Genomatica, Inc.

Inventor： Andreas W. Schirmer ,  Haibo Wang ,  Stephen B. Del Cardayre ,  Zhihao Hu ,  Louis G. Hom ,  Baolong Zhu ,  Cindy Chang ,  Emanuela E. Popova

IPC: C12P7/6409 ,  C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N15/52 ,  C12N15/62 ,  C12P7/18 ,  C12P7/42 ,  C12P7/6436

CPC classification number: C12P7/6409 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0071 ,  C12N9/0077 ,  C12N15/52 ,  C12N15/62 ,  C12P7/18 ,  C12P7/42 ,  C12P7/6436 ,  C12Y114/14001 ,  C12Y114/15003 ,  C07K2319/00 ,  C12Y101/00 ,  C12Y102/01042 ,  Y02E50/10

Abstract: The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.

公开(公告)号：US20240052332A1

公开(公告)日：2024-02-15

申请号：US18266238

申请日：2021-12-08

Applicant: Genomatica, Inc.

Inventor： Michael A. Noble ,  Sarah Skye McNees ,  Darlene Jan Abcede Usi ,  Joseph R. Warner

IPC: C12N9/88 ,  C12P7/42

CPC classification number: C12N9/88 ,  C12Y404/01026 ,  C12P7/42

Abstract: Described herein are non-natural olivetolic acid cyclases (OAC) variants capable of forming a 2,4-dihydroxy-6-alkylbenzoic acid from a 3,5,7-trioxoacyl-CoA or a 3,5,7-trioxocarboxylate substrate at a greater rate than a wild type or control OAC. The non-natural OAC (and OLS) can be expressed in an engineered cell having a pathway to form cannabinoids, which include CBGA, its analogs and derivatives. CBGA can be used for the preparation of cannabigerol (CBG), which can be used in therapeutic compositions.

公开(公告)号：US20230399688A1

公开(公告)日：2023-12-14

申请号：US18120951

申请日：2023-03-13

Applicant: GENOMATICA, INC.

Inventor： Stephanie CULLER ,  Ihsiung Brandon CHEN ,  Priti PHARKYA ,  Steve VAN DIEN ,  Nelson BARTON

IPC: C12Q1/6869 ,  A61K35/66 ,  C12P21/02 ,  C12P19/34 ,  A61K36/02 ,  A61K36/06 ,  C12N5/16 ,  C12N15/09 ,  C40B40/04

CPC classification number: C12Q1/6869 ,  A61K35/66 ,  C12P21/02 ,  C12P19/34 ,  A61K36/02 ,  A61K36/06 ,  C12N5/16 ,  C12N15/09 ,  C40B40/04 ,  Y02A50/30 ,  A61K2236/15 ,  A61K2236/30 ,  C12N2330/50

Abstract: In alternative embodiments, provided herein are transcription/translation (TX-TL) systems and methods of using them for use as rapid prototyping platforms for the synthesis, modification and identification of natural products (NPs), and natural product analogs (NPAs) and secondary metabolites, from biosynthetic gene cluster pipelines. In alternative embodiments, exemplary TX-TL systems as provided herein are used for the combinatorial biosynthesis of natural products (NPs), natural product analogs (NPAs) and secondary metabolites. In alternative embodiments, exemplary TX-TL systems as provided herein are used for the rapid prototyping of complex biosynthetic pathways as a way to rapidly assess combinatorial and biosynthetic designs before moving to cellular hosts. In alternative embodiments, these exemplary TX-TL systems are multiplexed for high-throughput (HT) automation and for prototyping engineered platforms for the synthesis or modification of natural products (NPs), and natural product analogs (NPAs) and secondary metabolites analogs.

公开(公告)号：US20230332193A1

公开(公告)日：2023-10-19

申请号：US18043258

申请日：2021-09-01

Applicant: GENOMATICA, INC.

Inventor： Andreas W. SCHIRMER ,  Deqiang ZHANG ,  Jamison Parker HUDDLESTON

IPC: C12P17/06 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/52

CPC classification number: C12P17/06 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y203/01206 ,  C12N9/88 ,  C12Y404/01026 ,  C12N9/1085 ,  C12Y205/0101 ,  C12N15/52

Abstract: The disclosure relates to a non-natural flavin-dependent oxidase comprising at least one amino acid variation as compared to a wild type flavin-dependent oxidase, wherein the non-natural flavin-dependent oxidase does not comprise a disulfide bond, and wherein the non-natural flavin-dependent oxidase is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid. The disclosure also relates to a nucleic acid, an expression construct, and an engineered cell for making the non-natural flavin-dependent oxidase. Also provided are compositions comprising the non-natural flavin-dependent oxidase; isolated non-natural flavin-dependent oxidase and methods of making the same; cell extracts comprising the non-natural flavin-dependent oxidase; and methods of making cannabinoids.

公开(公告)号：US11708589B2

公开(公告)日：2023-07-25

申请号：US16894600

申请日：2020-06-05

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

IPC: C12P7/18 ,  C12N15/52

CPC classification number: C12P7/18 ,  C12N15/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

公开(公告)号：US20230174454A1

公开(公告)日：2023-06-08

申请号：US17998111

申请日：2021-05-10

Applicant: Genomatica, Inc.

Inventor： Bo CHEN ,  Iman SAFARI ,  Seth LEVINE

IPC: C07C67/08 ,  C07C67/54 ,  C12P7/18

CPC classification number: C07C67/08 ,  C07C67/54 ,  C12P7/18

Abstract: A process for synthesizing (R)-3 -hy droxybutyl (R)-3 -hy droxybutanoate from ethyl (R)-3-hydroxybutanoate and (R)-1,3-butanediol, as well a process for synthesizing (R)-3-hy droxybutyl (R)-3-hy droxybutanoate from (R)-3-hydroxybutyric acid and (R)-1,3-butanediol. Also provided are processes for isolating (R)-3-hy droxybutyl (R)-3-hy droxybutanoate, including from a fermentation broth.

公开(公告)号：US20230136909A1

公开(公告)日：2023-05-04

申请号：US17833695

申请日：2022-06-06

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12N15/52 ,  C12P7/42 ,  C08F120/06 ,  C08G63/664 ,  C12N15/63 ,  C12P7/24 ,  C12P7/40 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12P7/62

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.

公开(公告)号：US20230022583A1

公开(公告)日：2023-01-26

申请号：US17831011

申请日：2022-06-02

Applicant: Genomatica, Inc.

Inventor： Liang Wu ,  Axel Christoph Trefzer ,  Stefaan Marie Andre De Wildeman ,  Marco Alexander Van Den Berg

IPC: C12P7/44 ,  C12N15/70 ,  C12P7/62 ,  C12P17/10

Abstract: The present invention relates to a method for preparing an adipate ester or thioester. The invention further relates to a method for preparing adipic acid from said ester or thioester. Further the invention provides a number of methods for preparing an intermediate for said ester or thioester. Further the invention relates to a method for preparing 6-amino caproic acid (6-ACA), a method for preparing 5-formyl valeric acid (5-FVA), and a method for preparing caprolactam. Further, the invention relates to a host cell for use in a method according to the invention.

公开(公告)号：US11535874B2

公开(公告)日：2022-12-27

申请号：US14436722

申请日：2013-10-21

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12N9/96 ,  C12P7/46 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C07C55/10 ,  C08G63/00 ,  C12N15/70 ,  C12N15/81 ,  C12P7/24 ,  C07C59/235 ,  C12N15/74 ,  C12N15/77

Abstract: Provided herein is a non-naturally occurring microbial organism (NNOMO) having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as succinate. Also provided herein are methods for using such an organism to produce succinate.

公开(公告)号：US20220403420A1

公开(公告)日：2022-12-22

申请号：US17770233

申请日：2020-10-22

Applicant: Genomatica, Inc.

Inventor： Harish Nagarajan ,  Gionata Scalcinati ,  Tae Hoon Yang

IPC: C12P7/18 ,  C12N9/02 ,  C12N9/12 ,  C12N9/04

Abstract: The present disclosure provides microbial organisms having increased availability of co-factors, such as NADPH, for increasing production of various products, including 1,3-BDO, MMA, (3R)-hydroxybutyl (3R)-hydroxybutyrate, amino acids, 3HB-CoA, adipate, caprolactam, 6-ACA, HMD A, or MAA, and products made from any of these. Also provided are one or more exogenous nucleic acids encoding an enzyme expressed in a sufficient amount to increase availability of NADPH, where the exogenous nucleic acid includes one or more of ATP-NADH kinase, pntAB, nadK, and gapN. Also provided are one or more gene attenuations occurring in genes, such as NDH-2, that result in an increased ratio of NADPH to NADH. Various combinations of the exogenous nucleic acids and gene deletions are also provided in the present disclosure. The present disclosure also provides methods of making and using the same, including methods for culturing cells, and for the production of the various products.