公开(公告)号：US20240076658A1

公开(公告)日：2024-03-07

申请号：US18214941

申请日：2023-06-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Jeremy AGRESTI

IPC: C12N15/10

CPC classification number: C12N15/1096 ,  C12N15/1065

Abstract: Methods and compositions are provided herein for preparing high-throughput cDNA sequencing libraries.

公开(公告)号：US11905551B2

公开(公告)日：2024-02-20

申请号：US17508674

申请日：2021-10-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jeremy Agresti ,  George Karlin-Neumann

IPC: C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6832 ,  C12Q1/682

CPC classification number: C12Q1/6806 ,  C12Q1/682 ,  C12Q1/686 ,  C12Q1/6811 ,  C12Q1/6832 ,  C12Q1/6853 ,  C12Q1/6853 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2531/119 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/537

Abstract: Improved multiple displacement amplification (MDA) reagents and methods are provided.

公开(公告)号：US11866766B2

公开(公告)日：2024-01-09

申请号：US18048512

申请日：2022-10-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  John Stark ,  Yadwinder Deol ,  Vishal Sharma

IPC: C12Q1/6811 ,  C12Q1/6874 ,  C12N15/10

CPC classification number: C12Q1/6811 ,  C12N15/1065 ,  C12Q1/6874 ,  C12Q2563/179 ,  C12Q2565/629 ,  C12Q2600/178

Abstract: A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.

公开(公告)号：US11834710B2

公开(公告)日：2023-12-05

申请号：US17136824

申请日：2020-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jennifer Chew

IPC: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6862 ,  C12Q1/6876 ,  C12Q1/6848 ,  C12Q1/686 ,  C12N15/10 ,  C12N9/12 ,  C12Q1/6806

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12N15/1003 ,  C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/6855 ,  C12Q1/6862 ,  C12Q1/6876 ,  C12Q2563/185 ,  C12Y207/07007 ,  C12Q1/6806 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: The methods and reagents are provided for barcoding and analysis of DNA samples using partition (e.g., droplet) technology while avoiding performing amplification in droplets.

公开(公告)号：US11834685B2

公开(公告)日：2023-12-05

申请号：US17225189

申请日：2021-04-08

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Xiao-Song Gong

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C12Y207/07007 ,  C07K2319/00 ,  C12N2740/13022

Abstract: Covalently-linked DNA polymerases are provided.

公开(公告)号：US20230372926A1

公开(公告)日：2023-11-23

申请号：US18227218

申请日：2023-07-27

Applicant: Bio-Rad Laboratories, Inc. ,  Bio-Rad Europe GmbH

Inventor： Lorenzo Franco Teodoro Petrini ,  Iago Pereiro Pereiro ,  Robert Dean Lovchik ,  Anna Fomitcheva Khartchenko ,  Anel Zulji ,  Govind Kaigala ,  Cristina Boero ,  Olivier Michel Christian Noël ,  Christophe Guex ,  Stéphane Guillod ,  Aurélie Bolon

IPC: B01L3/00

CPC classification number: B01L3/5027 ,  B01L13/02 ,  B01L2300/14 ,  B01L2200/0642

Abstract: A method comprises providing a microfluidic device including a reservoir; an injection nozzle at the bottom of the reservoir, the injection nozzle being wider at its top than at its bottom; an injection channel below the injection nozzle; and a microfluidic channel below the injection channel. The method further comprises placing fluid in the reservoir, and allowing the fluid to passively flow through the injection nozzle and the injection channel.

公开(公告)号：US20230340627A1

公开(公告)日：2023-10-26

申请号：US18296357

申请日：2023-04-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Polina TSIGANKOV ,  Dalia SHEZIFI ,  Galina FIHMAN ,  Matt DUNN ,  Brigid CUNNION

IPC: C12Q1/70 ,  G01N33/569

CPC classification number: C12Q1/701 ,  G01N33/56983 ,  C12Q1/6851

Abstract: Methods, compositions, and kits for analyzing viral particles. In an exemplary method of analyzing viral particles, capsids of the viral particles may be tagged with a tag. Subsamples of a sample containing the viral particles may be formed. Each subsample of only a subset of the subsamples may include at least one of the viral particles. One or more targets may be amplified from a genome of the viral particles. Tag-related data, and amplification data for the one or more targets, may be collected from the subsamples. In some examples, a capsid occupancy of the viral particles may be determined in a calibration-free approach using the collected data without quantification of the viral particles or capsids thereof.

公开(公告)号：US20230323435A1

公开(公告)日：2023-10-12

申请号：US18185220

申请日：2023-03-16

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Vishal SHARMA ,  Ronald LEBOFSKY

IPC: C12Q1/6837 ,  C12N15/10

CPC classification number: C12Q1/6837 ,  C12N15/1065

Abstract: The methods allow for provision of a mixture of a plurality of beads, each bead linked to oligonucleotides, wherein the mixture can be treated as a bulk solution (prior to partitioning) to cleave a covalent bond linking the oligonucleotides to the beads while retaining a non-covalent linkage (via hybridization) between the beads and the oligonucleotides, allowing for distribution of the oligonucleotides and beads to partitions or 2D arrays prior to separation of the oligonucleotides from the beads, which occurs for example in the partitions.

公开(公告)号：US11759780B2

公开(公告)日：2023-09-19

申请号：US17153311

申请日：2021-01-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Joshua Ritchey ,  Yi Xue ,  Robert Meltzer

IPC: B01L3/00 ,  C09D183/04 ,  C09D183/00 ,  B81C1/00 ,  C12Q1/686

CPC classification number: B01L3/50273 ,  B01L3/502707 ,  B01L3/502784 ,  B81C1/00206 ,  C09D183/00 ,  C09D183/04 ,  B01L2200/06 ,  B01L2300/12 ,  B01L2300/165 ,  B01L2400/04 ,  C12Q1/686 ,  C09D183/04 ,  C08K3/36

Abstract: Compositions, devices, and methods are disclosed for the modification of polymer surfaces with coatings having a dispersion of silicone polymer and hydrophobic silica. The surface coatings provide the polymer surface with high hydrophobicity, as well as increased resistance to biofouling with proteinaceous material. The polymer surfaces can be particularly useful in microfluidic devices and methods that involve the contacting of the covalently modified polymer surfaces with emulsions of aqueous droplets containing biological macromolecules within an oil carrier phase.

公开(公告)号：US11759761B2

公开(公告)日：2023-09-19

申请号：US17381096

申请日：2021-07-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Nicholas Heredia

IPC: B01J19/00 ,  C12Q1/6806

CPC classification number: B01J19/0046 ,  C12Q1/6806 ,  B01J2219/00547 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12Q1/6806 ,  C12Q2525/131 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q2565/519 ,  C12Q1/6806 ,  C12Q2523/319 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q2565/519 ,  C12Q1/6806 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q2565/519

Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.