公开(公告)号：US11965891B2

公开(公告)日：2024-04-23

申请号：US15393445

申请日：2016-12-29

Applicant: Bio-Rad Laboratories, Inc. ,  Bio-Rad Europe Gmbh

Inventor： Jeremy Agresti ,  Ronald Lebofsky

IPC: G01N33/68 ,  B01J19/00 ,  C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6869 ,  C40B20/04 ,  C40B70/00

CPC classification number: G01N33/6878 ,  B01J19/0046 ,  C12Q1/6816 ,  C12Q1/6869 ,  C40B20/04 ,  C40B70/00 ,  C12Q1/6816 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q1/6869 ,  C12Q2565/514

Abstract: Methods and compositions are described for single cell resolution, quantitative proteomic analysis using high throughput sequencing.

公开(公告)号：US20240124856A1

公开(公告)日：2024-04-18

申请号：US18449107

申请日：2023-08-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12N9/12

CPC classification number: C12N9/1276 ,  C12Y207/07049 ,  C07K2319/20

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.

公开(公告)号：US11946855B2

公开(公告)日：2024-04-02

申请号：US18195084

申请日：2023-05-09

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique

IPC: G01N15/14 ,  B01L3/00 ,  B01L3/02 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/00 ,  G01N15/10 ,  G01N33/487 ,  G06V20/69 ,  G01N15/02 ,  G01N35/00

CPC classification number: G01N15/1484 ,  B01L3/021 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  C12M47/04 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N1/405 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/086 ,  G01N1/4077 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2035/00158 ,  G06V20/69

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

公开(公告)号：US11939573B2

公开(公告)日：2024-03-26

申请号：US16935754

申请日：2020-07-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Serge Saxonov

IPC: C12N15/10 ,  C12Q1/6876

CPC classification number: C12N15/1065 ,  C12N15/10 ,  C12N15/1075 ,  C12Q1/6876 ,  C12N15/10 ,  C12Q2525/191 ,  C12Q2563/179

Abstract: Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.

公开(公告)号：US20240093271A1

公开(公告)日：2024-03-21

申请号：US18514645

申请日：2023-11-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US20240076658A1

公开(公告)日：2024-03-07

申请号：US18214941

申请日：2023-06-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Jeremy AGRESTI

IPC: C12N15/10

CPC classification number: C12N15/1096 ,  C12N15/1065

Abstract: Methods and compositions are provided herein for preparing high-throughput cDNA sequencing libraries.

公开(公告)号：US11905551B2

公开(公告)日：2024-02-20

申请号：US17508674

申请日：2021-10-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jeremy Agresti ,  George Karlin-Neumann

IPC: C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6832 ,  C12Q1/682

CPC classification number: C12Q1/6806 ,  C12Q1/682 ,  C12Q1/686 ,  C12Q1/6811 ,  C12Q1/6832 ,  C12Q1/6853 ,  C12Q1/6853 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2531/119 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/537

Abstract: Improved multiple displacement amplification (MDA) reagents and methods are provided.

公开(公告)号：US11866766B2

公开(公告)日：2024-01-09

申请号：US18048512

申请日：2022-10-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  John Stark ,  Yadwinder Deol ,  Vishal Sharma

IPC: C12Q1/6811 ,  C12Q1/6874 ,  C12N15/10

CPC classification number: C12Q1/6811 ,  C12N15/1065 ,  C12Q1/6874 ,  C12Q2563/179 ,  C12Q2565/629 ,  C12Q2600/178

Abstract: A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.

公开(公告)号：US11834710B2

公开(公告)日：2023-12-05

申请号：US17136824

申请日：2020-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jennifer Chew

IPC: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6862 ,  C12Q1/6876 ,  C12Q1/6848 ,  C12Q1/686 ,  C12N15/10 ,  C12N9/12 ,  C12Q1/6806

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12N15/1003 ,  C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/6855 ,  C12Q1/6862 ,  C12Q1/6876 ,  C12Q2563/185 ,  C12Y207/07007 ,  C12Q1/6806 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: The methods and reagents are provided for barcoding and analysis of DNA samples using partition (e.g., droplet) technology while avoiding performing amplification in droplets.

公开(公告)号：US11834685B2

公开(公告)日：2023-12-05

申请号：US17225189

申请日：2021-04-08

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Xiao-Song Gong

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C12Y207/07007 ,  C07K2319/00 ,  C12N2740/13022

Abstract: Covalently-linked DNA polymerases are provided.