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53 条记录 (耗时 0.05 秒)

    Systems and methods for performing reactions in an unsealed environment
    22.
    发明授权
    Systems and methods for performing reactions in an unsealed environment 有权
    在非密封环境中进行反应的系统和方法

    公开(公告)号：US06225061B1

    公开(公告)日：2001-05-01

    申请号：US09266409

    申请日：1999-03-10

    申请人： Thomas Becker ,  Hubert Köster ,  Charles Cantor

    发明人： Thomas Becker ,  Hubert Köster ,  Charles Cantor

    IPC分类号： C12Q168

    CPC分类号： B01J19/16 ,  B01J2219/00317 ,  B01J2219/00367 ,  B01J2219/00378 ,  B01J2219/00495 ,  B01J2219/00603 ,  B01J2219/0068 ,  C40B60/14 ,  G01N2035/00287 ,  Y10T436/11 ,  Y10T436/25375

    摘要： An open system is provided for performing a submicroliter reaction. An open system can contain a solid support having a target site for performing the reaction; a liquid dispensing system such as a nanoliter dispensing pipette for dispensing a submicroliter amount of a liquid to the target site; a temperature controlling device for regulating the temperature of the support; and means for controlling the amount of liquid dispensed, which corresponds to the amount of liquid that evaporates from the target site. Also provided is an open system, including a solid support having a target site; a liquid dispensing system, which can dispense a liquid to the target site; a temperature controlling system, which regulates the temperature of the solid support; and an interface, which regulates an amount of liquid dispensed from the liquid dispensing system. Also provided is a method for performing a reaction in a submicroliter volume in an unsealed environment by dispensing a submicroliter volume of liquid onto the surface of a support; monitoring the temperature of the support; monitoring an amount or rate of evaporation of the liquid; and dispensing to the surface of the support a further amount of the liquid, which corresponds to the amount lost from the support due to evaporation, thereby maintaining the reaction volume at a predetermined volume throughout the course of the reaction. A method also is provided for maintaining a volume of a reaction mixture, which can be one of a plurality of reaction mixtures, on a solid support in an unsealed environment by monitoring the rate of evaporation of a liquid from the reaction mixture; and dispensing into the reaction mixture an amount of liquid that corresponds to the amount that evaporates.

    摘要翻译： 提供了用于进行亚微米级反应的开放系统。 开放系统可以含有具有用于进行反应的靶位点的固体支持物; 液体分配系统，例如用于将目标部位的微量剂量的液体分配的纳升分配移液管; 用于调节支撑件的温度的温度控制装置; 以及用于控制分配的液体量的装置，其对应于从目标部位蒸发的液体的量。 还提供了一种开放系统，包括具有目标地点的固体支撑物; 液体分配系统，其可以将液体分配到目标部位; 温度控制系统，其调节固体支持物的温度; 以及界面，其调节从液体分配系统分配的液体的量。 还提供了一种在非密封环境中通过将亚微米级体积的液体分配到载体的表面上来进行亚微米体积的反应的方法; 监测支架的温度; 监测液体的蒸发量或速率; 并且向支撑体的表面分配更多量的液体，其对应于由于蒸发而从支撑物损失的量，从而在反应过程中将反应体积保持在预定体积。 还提供了一种方法，用于通过监测来自反应混合物的液体的蒸发速率来保持反应混合物的体积，反应混合物可以是多个反应混合物中的一种，在固体支持物上在未密封环境中; 并向反应混合物中分配一定量的与蒸发量对应的液体。

    Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
    25.
    发明授权
    Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions 有权
    捕获化合物，其收集物和分析蛋白质组合物和复合物组合物的方法

    公开(公告)号：US07858560B2

    公开(公告)日：2010-12-28

    申请号：US10197954

    申请日：2002-07-16

    申请人： Hubert Köster ,  Suhaib Siddiqi ,  Daniel P. Little

    发明人： Hubert Köster ,  Suhaib Siddiqi ,  Daniel P. Little

    IPC分类号： C40B44/00

    CPC分类号： G01N33/6803 ,  B01J2219/00576 ,  B01J2219/00581 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  C12Q1/6834 ,  C40B40/06 ,  C40B40/10 ,  G01N33/6842 ,  G01N33/6848 ,  G01N33/6851 ,  C12Q2565/627 ,  C12Q2565/518

    摘要： Capture Collections of capture compounds and collections thereof and methods using the compounds collections for the analysis of biomolecules are provided. In particular, collections, compounds and methods are provided for analyzing complex protein mixtures, such as the proteome. The compounds in the collections are multifunctional reagents that provide for the separation and isolation of biomolecules in complex protein mixtures. In the collections, each and every compound in the collection, has the formula: Z is a trityl derivative, each of m and n independently is an integer that is 1 to 100; X, the reactivity function, covalently binds to amino acid side chains of biomolecules; Y, the selectivity function, modulates binding of X to the amino acid side chains in biomolecules such that X binds to fewer biomolecules when the selectivity moiety Y is present than in its absence; and Q permits separation or immobilization of capture compounds in the collection. Automated systems for performing the methods also are provided.

    摘要翻译： 捕获捕获化合物及其收集物的集合以及使用化合物集合分析生物分子的方法。 特别地，提供收集，化合物和方法用于分析复合蛋白质混合物，例如蛋白质组。 收集物中的化合物是多功能试剂，其提供复杂蛋白质混合物中生物分子的分离和分离。 在集合中，集合中的每个化合物具有以下分子式：Z是三苯甲基衍生物，m和n各自独立地是1至100的整数; X，反应性功能共价结合生物分子的氨基酸侧链; Y，选择性功能，调节X与生物分子中氨基酸侧链的结合，使得当存在选择性部分Y时，X与不存在的选择性部分Y结合较少的生物分子; 并且Q允许在收集物中分离或固定捕获化合物。 还提供了用于执行方法的自动化系统。

    Solution phase biopolymer synthesis
    28.
    发明授权
    Solution phase biopolymer synthesis 失效
    溶液相生物聚合物合成

    公开(公告)号：US07038103B2

    公开(公告)日：2006-05-02

    申请号：US09484484

    申请日：2000-01-18

    申请人： Hubert Köster ,  Ralf Wörl

    发明人： Hubert Köster ,  Ralf Wörl

    IPC分类号： C07C15/02 ,  C07C7/00

    CPC分类号： C07H21/00 ,  C07B2200/11 ,  C07C25/18 ,  C07C43/225 ,  C07C69/708 ,  C07C69/76 ,  C07C211/14 ,  C07C233/78 ,  C07C235/10 ,  C07C255/16 ,  C07K1/02 ,  C07K14/003 ,  Y10S585/93 ,  Y10S585/932 ,  Y10S585/933 ,  Y10S585/934 ,  Y10S585/935

    摘要： Multifunctional liquid phase carriers (LPCs) and methods of using LPCs for the preparation of biopolymers are provided. The LPCs are highly symmetrical compounds that possess more than two points of attachment for biopolymer synthesis. The LPCs have the formula Sp(X1)n, where Sp is a highly symmetrical moiety such that all X1 groups are equivalent. X1 is a functional group that is suitable for biopolymer synthesis, including OH, SH, NH2, COOH and the like. Biopolymers that may be produced using the methods provided include oligonucleotides, peptides, protein nucleic acids (PNAs) and oligosaccharides. Analogs of the biopolymers may also be prepared using the methods.

    摘要翻译： 提供了多功能液相载体（LPCs）和使用LPC制备生物聚合物的方法。 LPC是高度对称的化合物，其具有用于生物聚合物合成的两个以上连接点。 LPC具有式Sp（X 1）N n，其中Sp是高度对称的部分，使得所有X 1个基团相当。 X 1是适于生物聚合物合成的官能团，包括OH，SH，NH 2，COOH等。 可以使用提供的方法产生的生物聚合物包括寡核苷酸，肽，蛋白质核酸（PNA）和寡糖。 生物聚合物的类似物也可以使用这些方法制备。

    Mass spectrometric detection of polypeptides
    29.
    发明授权
    Mass spectrometric detection of polypeptides 有权
    多肽的质谱检测

    公开(公告)号：US06322970B1

    公开(公告)日：2001-11-27

    申请号：US09146054

    申请日：1998-09-02

    申请人： Daniel Little ,  Hubert Köster ,  G. Scott Higgins ,  David Lough

    发明人： Daniel Little ,  Hubert Köster ,  G. Scott Higgins ,  David Lough

    IPC分类号： C12Q168

    CPC分类号： G01N33/6848 ,  G01N33/6818 ,  G01N33/6842 ,  G01N33/6851 ,  H01J49/00

    摘要： A process for determining the identity of a target polypeptide using mass spectroscopy is provided. Depending on the target polypeptide to be identified, a process as disclosed can be used, for example, to diagnose a genetic disease or chromosomal abnormality, a predisposition to a disease or condition, or infection by a pathogenic organism; or for determining identity or heredity. Kits for performing the disclosed processes also are provided.

    摘要翻译： 提供了使用质谱法确定靶多肽的身份的方法。 根据待鉴定的靶多肽，可以使用所公开的方法，例如诊断遗传疾病或染色体异常，疾病或病症的倾向或病原生物的感染; 或用于确定身份或遗传。 还提供了用于执行所公开的处理的套件。