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公开(公告)号:US20160370317A9
公开(公告)日:2016-12-22
申请号:US14498418
申请日:2014-09-26
IPC分类号: G01N27/447
CPC分类号: G01N27/44756 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L2300/0816 , B01L2300/0819 , B01L2400/0427 , B01L2400/0688 , B01L2400/086 , B29C59/02 , B41J2/1601 , B81B7/0061 , B81B2201/058 , B81B2201/13 , F04B17/00 , F04B19/006
摘要: The invention provides droplet actuators with droplet operations surfaces for manipulating droplets, e.g., by conducting droplet operations. The droplet operations surfaces are typically exposed to a droplet operations gap. One or more regions of a droplet operation surface may include patterned topographic features. The invention also provides a droplet actuator in which one or both gap-facing droplet operations surfaces is formed using a removable film. The removable film may, in various embodiments, also include other components ordinarily associated with the droplet actuator substrate, such as the dielectric layer and the electrodes. Further, the invention provides droplet actuator devices and methods for coupling and/or sealing substrates of a droplet actuator, such as techniques for self-aligning assembly of droplet actuator substrates. The invention provides droplet actuators and methods of disassembling the droplet actuator in order to provide access for cleaning and/or recycling of droplet actuator surfaces.
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22.
公开(公告)号:US09494498B2
公开(公告)日:2016-11-15
申请号:US14978935
申请日:2015-12-22
IPC分类号: B01L3/00 , B01L3/02 , G01N1/10 , G01N15/06 , G01N33/00 , G01N33/48 , G01N1/38 , B01F11/00 , B01F13/00 , G01N33/543 , B01L7/00 , G01N35/10
CPC分类号: G01N33/54326 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0809 , B01L3/50273 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0673 , B01L2300/06 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N1/38 , G01N2001/386 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
摘要翻译: 本发明提供了一种在液滴致动器中的液滴内循环磁响应珠的方法。 本发明还提供了分裂液滴的方法。 在一个实施例中,本发明利用具有顶部和底部基底的液滴致动器,分别存在于顶部和底部基底附近的多个磁场,其中至少一个磁场可选择性地改变,并且多个液滴 沿着顶表面和底表面中的至少一个定位的操作电极。 液滴位于顶表面和底表面之间,并且至少一个磁场被选择性地改变。
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公开(公告)号:US09395329B2
公开(公告)日:2016-07-19
申请号:US14805532
申请日:2015-07-22
IPC分类号: G01N1/18 , G01N35/10 , G01N27/447 , G01N15/00
CPC分类号: G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
摘要: The present invention relates to droplet-based particle sorting. According to one embodiment, a method of providing a droplet comprising a single cell is provided, wherein the method includes providing a droplet comprising a suspension of cells on an electrowetting droplet actuator surrounded by an oil medium, dispensing from the droplet to provide a dispensed droplet, determining whether the dispensed droplet on the electrowetting droplet actuator comprises a single cell, and sorting the dispensed droplet on the electrowetting droplet actuator based on the results of the determining step using electrowetting-mediated droplet operations.
摘要翻译: 本发明涉及基于液滴的颗粒分选。 根据一个实施例,提供了一种提供包括单个电池的液滴的方法,其中所述方法包括提供液滴,其包含由油介质包围的电润湿液滴致动器上的悬浮液,从液滴分配以提供分配的液滴 确定电润湿液滴致动器上的分配液滴是否包括单个电池,并且基于使用电润湿介导的液滴操作的确定步骤的结果,将分配的液滴分配在电润湿液滴致动器上。
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公开(公告)号:US09321049B2
公开(公告)日:2016-04-26
申请号:US14338889
申请日:2014-07-23
CPC分类号: B01L3/50273 , B01L3/502715 , B01L3/502784 , B01L2200/0605 , B01L2300/0627 , B01L2300/0636 , B01L2300/0645 , B01L2300/0809 , B01L2400/0427 , G01N27/22 , G01N27/223 , G01N27/227 , G01N2203/00 , G01V3/02
摘要: A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.
摘要翻译: 一种用于检测液滴致动器上电容的方法,电路和装置,特别是用于通过以下步骤确定液滴致动器上的电极处的液滴的存在,部分存在或不存在:(a)提供液滴致动器,其包括:(i) 基板,其包括布置在所述基板上的电极,用于在所述基板的表面上进行液滴操作; (ii)电容检测电路,用于检测一个或多个电极处的液滴操作表面处的电容; (b)在一个或多个电极处检测液滴操作表面处的电容; 和(c)根据电容确定在电极处的液滴操作表面处的液滴的存在,部分存在或不存在。
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公开(公告)号:US20160096174A1
公开(公告)日:2016-04-07
申请号:US14968094
申请日:2015-12-14
CPC分类号: B01L3/50273 , B01L3/502715 , B01L3/502784 , B01L2200/0605 , B01L2300/0627 , B01L2300/0636 , B01L2300/0645 , B01L2300/0809 , B01L2400/0427 , G01N27/22 , G01N27/223 , G01N27/227 , G01N2203/00 , G01V3/02
摘要: A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.
摘要翻译: 一种用于检测液滴致动器上电容的方法,电路和装置,特别是用于通过以下步骤确定液滴致动器上的电极处的液滴的存在,部分存在或不存在:(a)提供液滴致动器,其包括:(i) 基板,其包括布置在所述基板上的电极,用于在所述基板的表面上进行液滴操作; (ii)电容检测电路,用于检测一个或多个电极处的液滴操作表面处的电容; (b)在一个或多个电极处检测液滴操作表面处的电容; 和(c)根据电容确定在电极处的液滴操作表面处的液滴的存在,部分存在或不存在。
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26.
公开(公告)号:US20150285792A1
公开(公告)日:2015-10-08
申请号:US14746276
申请日:2015-06-22
IPC分类号: G01N33/543
CPC分类号: G01N33/54326 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0809 , B01L3/50273 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0673 , B01L2300/06 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N1/38 , G01N2001/386 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
摘要翻译: 本发明提供了一种在液滴致动器中的液滴内循环磁响应珠的方法。 本发明还提供了分裂液滴的方法。 在一个实施例中,本发明利用具有顶部和底部基底的液滴致动器,分别存在于顶部和底部基底附近的多个磁场,其中至少一个磁场可选择性地改变,并且多个液滴 沿着顶表面和底表面中的至少一个定位的操作电极。 液滴位于顶表面和底表面之间,并且至少一个磁场被选择性地改变。
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公开(公告)号:US09097662B2
公开(公告)日:2015-08-04
申请号:US14526928
申请日:2014-10-29
IPC分类号: G01N1/18 , G01N35/10 , G01N15/00 , G01N27/447
CPC分类号: G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
摘要: The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.
摘要翻译: 本发明涉及基于液滴的颗粒分选。 根据一个实施例,提供了液滴微致动器,其包括:(a)颗粒悬浮液; 和(b)布置成使用包含颗粒的液滴进行液滴操作的电极。 还提供了输送颗粒的方法,其中所述方法包括提供包含颗粒的液滴并将液滴输送到液滴微致动器上。
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公开(公告)号:US20150068903A1
公开(公告)日:2015-03-12
申请号:US14498418
申请日:2014-09-26
发明人: Vijay Srinivasan , Arjun Sudarsan , Vamsee K. Pamula , Michael G. Pollack , Ramakrishna Sista , Raymond Kozikowski , Nicole Bell
IPC分类号: G01N27/447
CPC分类号: G01N27/44756 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L2300/0816 , B01L2300/0819 , B01L2400/0427 , B01L2400/0688 , B01L2400/086 , B29C59/02 , B41J2/1601 , B81B7/0061 , B81B2201/058 , B81B2201/13 , F04B17/00 , F04B19/006
摘要: The invention provides droplet actuators with droplet operations surfaces for manipulating droplets, e.g., by conducting droplet operations. The droplet operations surfaces are typically exposed to a droplet operations gap. One or more regions of a droplet operation surface may include patterned topographic features. The invention also provides a droplet actuator in which one or both gap-facing droplet operations surfaces is formed using a removable film. The removable film may, in various embodiments, also include other components ordinarily associated with the droplet actuator substrate, such as the dielectric layer and the electrodes. Further, the invention provides droplet actuator devices and methods for coupling and/or sealing substrates of a droplet actuator, such as techniques for self-aligning assembly of droplet actuator substrates. The invention provides droplet actuators and methods of disassembling the droplet actuator in order to provide access for cleaning and/or recycling of droplet actuator surfaces.
摘要翻译: 本发明提供了液滴致动器,其具有用于操纵液滴的液滴操作表面,例如通过进行液滴操作。 液滴操作表面通常暴露于液滴操作间隙。 液滴操作表面的一个或多个区域可以包括图案化的地形特征。 本发明还提供一种液滴致动器,其中使用可去除的膜形成一个或两个面向间隙的液滴操作表面。 在各种实施例中,可除去膜可以包括通常与液滴致动器基底(例如电介质层和电极)相关联的其它部件。 此外,本发明提供了液滴致动器装置和用于联接和/或密封液滴致动器的基板的方法,例如用于液滴致动器基板的自对准组装的技术。 本发明提供了液滴致动器和拆卸液滴致动器的方法,以便为液滴致动器表面的清洁和/或回收提供通路。
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公开(公告)号:US20150034485A1
公开(公告)日:2015-02-05
申请号:US14466193
申请日:2014-08-22
IPC分类号: G01N33/561 , B03C1/30 , B03C5/00
CPC分类号: G01N33/54333 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B03C1/02 , B03C1/288 , B03C1/30 , B03C5/005 , B03C2201/18 , B03C2201/26 , C12N15/1013 , G01N33/561 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要: The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
摘要翻译: 本发明涉及在液滴致动器上的珠孵化和洗涤。 提供了用于将用一抗标记的磁响应珠,样品(即分析物)和二次报告基因抗体孵育在磁体上,磁铁上和从磁体上离开并完全离开磁体的方法。 还提供了使用形状辅助的液滴合并来洗涤磁响应珠的方法。 还提供了用于形状介导的分离,输送和分配含有磁响应珠的样品液滴的方法。 本发明的装置和方法提供了免疫测定中分析物的快速结果和最佳检测的时间。
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公开(公告)号:US10585090B2
公开(公告)日:2020-03-10
申请号:US16132175
申请日:2018-09-14
IPC分类号: G01N15/06 , G01N33/00 , G01N33/48 , G01N35/00 , G01N1/00 , G01N1/10 , B01L3/00 , G01N33/543 , B01F13/00 , B03C1/02 , B03C1/28 , B03C1/30 , B03C5/00 , C12N15/10 , G01N33/561 , B01F11/00 , B01L7/00 , G01N35/10
摘要: Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.
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