Application of electrical stimulation for functional tissue engineering in vitro and in vivo
    26.
    发明授权
    Application of electrical stimulation for functional tissue engineering in vitro and in vivo 有权
    电刺激在体外和体内功能组织工程中的应用

    公开(公告)号:US08367410B2

    公开(公告)日:2013-02-05

    申请号:US10872577

    申请日:2004-06-21

    IPC分类号: C12N5/00 C12N5/02 C12N5/07

    摘要: The present invention provides new methods for the in vitro preparation of bioartificial tissue equivalents and their enhanced integration after implantation in vivo. These methods include submitting a tissue construct to a biomimetic electrical stimulation during cultivation in vitro to improve its structural and functional properties, and/or in vivo, after implantation of the construct, to enhance its integration with host tissue and increase cell survival and functionality. The inventive methods are particularly useful for the production of bioartificial equivalents and/or the repair and replacement of native tissues that contain electrically excitable cells and are subject to electrical stimulation in vivo, such as, for example, cardiac muscle tissue, striated skeletal muscle tissue, smooth muscle tissue, bone, vasculature, and nerve tissue.

    摘要翻译: 本发明提供了体外制备生物人造组织当量的新方法及其在体内植入后的增强整合。 这些方法包括在体外培养过程中将组织构建物递送至仿生电刺激,以改善其结构和功能性质,和/或在植入该构建体后体内,以增强其与宿主组织的整合并增加细胞存活和功能。 本发明的方法特别可用于生产生物人造等同物和/或修复和替换含有电可兴奋细胞的天然组织,并且在体内受到电刺激,例如心肌组织,条纹骨骼肌组织 ,平滑肌肉组织,骨骼,脉管系统和神经组织。