公开(公告)号：US5690895A

公开(公告)日：1997-11-25

申请号：US761143

申请日：1996-12-06

申请人： Masaetsu Matsumoto ,  Isao Yamazaki ,  Ryo Miyake ,  Masaharu Ishii ,  Ryohei Yabe ,  Hiroshi Ohki ,  Hideyuki Horiuchi ,  Shinichi Sakuraba

发明人： Masaetsu Matsumoto ,  Isao Yamazaki ,  Ryo Miyake ,  Masaharu Ishii ,  Ryohei Yabe ,  Hiroshi Ohki ,  Hideyuki Horiuchi ,  Shinichi Sakuraba

IPC分类号： G01N15/00 ,  G01N15/02 ,  G01N15/06 ,  G01N15/14 ,  G01N21/05 ,  G01N33/49

CPC分类号： G01N21/05 ,  G01N15/1404 ,  G01N2021/0346

摘要： A flow cell apparatus, useful in an apparatus for measuring particles suspended in a liquid, comprises a sample liquid, supply device, a sheath liquid supply device, a flow cell which has a flow passage passing through a transparent measuring portion, and a nozzle for causing the sample liquid, from the supply device to flow in the flow passage of the flow cell. The nozzle has at least one discharge port which is disposed in the flow passage in a spaced relation from the inner wall of the flow cell flow passage. The sheath liquid supply device causes the sheath liquid to flow around the nozzle discharge port and surround the sample liquid flow to form a sheath flow. The flow passage of the flow cell and the nozzle are so formed as to ensure a sample liquid flow of a fixed width through the measuring portion. A stable sample flow having a fixed width can be formed in the measuring portion even when the flow is at a high velocity, and particles in the sample liquid can efficiently be photographed with high accuracy.

摘要翻译： 一种用于测量悬浮在液体中的颗粒的装置中的流动池装置，包括样品液体，供给装置，皮肤液体供应装置，具有通过透明测量部分的流动通道的流动池，以及用于 使来自供给装置的样品液体流入流动池的流路。 喷嘴具有至少一个排出口，该排出口以与流动池流动通道的内壁隔开的关系设置在流动通道中。 皮肤液体供给装置使皮肤液体在喷嘴排出口周围流动并且围绕样品液体流动形成鞘流。 流动池和喷嘴的流动通道形成为确保通过测量部分具有固定宽度的样品液体流动。 即使在流速高的情况下，也可以在测量部分形成具有固定宽度的稳定的样品流，并且可以高精度地有效地拍摄样品液体中的颗粒。

公开(公告)号：US4863264A

公开(公告)日：1989-09-05

申请号：US203457

申请日：1988-06-07

申请人： Ryo Miyake ,  Hiroshi Ohki ,  Isao Yamazaki

发明人： Ryo Miyake ,  Hiroshi Ohki ,  Isao Yamazaki

IPC分类号： G01N15/14 ,  G01N33/48

CPC分类号： G01N15/1404 ,  G01N2015/1409 ,  G01N2015/1452

摘要： A photoanalysis apparatus includes marker pouring device for pouring markers into one of sheath fluid and fluid carrying particles in a flow-cell device, second optical device for applying a light beam for correction to the markers in the flow-cell device to measure strength of fluorescence or light scattering caused by said markers to generate a signal for correction, second signal processing device for calculating from the signal for correction at least one of a difference in position between a center of the fluid carrying particles in the flow-cell device and a center of a light beam for examination and a difference in flow rate between a predetermined flow rate and an actual flow rate of the fluid carrying particles in the flow-cell device to generate correspondingly at least one of a first command signal for position correction and a second command signal for flow rate correction, and correspondingly at least one of first correcting device for shifting the center of the light beam for examination to the center of the flow of the fluid carrying particles in accordance with the first command signal and second correcting device for correcting the flow rate of the fluid carrying particles to the predetermined flow rate in accordance with the second command signal.

公开(公告)号：US08658102B2

公开(公告)日：2014-02-25

申请号：US12785835

申请日：2010-05-24

申请人： Hajime Katou ,  Ryo Miyake ,  Hiroyasu Uchida ,  Katsuhiro Kambara ,  Takenori Okusa ,  Shigenori Watari ,  Yoichiro Suzuki ,  Tomonori Mimura ,  Miki Taki ,  Akira Inagaki

发明人： Hajime Katou ,  Ryo Miyake ,  Hiroyasu Uchida ,  Katsuhiro Kambara ,  Takenori Okusa ,  Shigenori Watari ,  Yoichiro Suzuki ,  Tomonori Mimura ,  Miki Taki ,  Akira Inagaki

IPC分类号： B06B1/06

CPC分类号： G01N35/1002 ,  B01F11/0283 ,  B01F2215/0037 ,  G01N35/025 ,  G01N2035/00554 ,  Y10S707/962 ,  Y10S707/99933 ,  Y10S707/99942 ,  Y10S707/99943 ,  Y10S707/99956

摘要： A chemical analysis apparatus comprising reaction containers containing therein a substance to be analyzed; an agitating mechanism spaced from the substance to be analyzed and agitating said substance to be analyzed with a liquid in said reaction container; and, a measuring portion for measuring physical properties of the substance to be analyzed, said agitating mechanism having a sound supply portion supplying sound waves to the substance to be analyzed, wherein said sound supply portion comprises a mechanism changing, in time, intensity of ultrasonic waves to be irradiated so as to apply pulsation to a swirl flow in the reaction container.

摘要翻译： 一种化学分析装置，包括含有待分析物质的反应容器; 与待分析物质间隔开的搅拌机构，并在所述反应容器中用液体搅拌待分析物质; 以及用于测量被分析物质的物理性质的测量部分，所述搅拌机构具有向待分析物质供应声波的声音供给部分，其中所述声音供应部分包括时间上改变超声波强度的机构 要被照射的波浪以便对反应容器中的涡流施加脉动。

公开(公告)号：US06875984B2

公开(公告)日：2005-04-05

申请号：US10621444

申请日：2003-07-18

申请人： Hiroshi Kakibayashi ,  Shigeyuki Hosoki ,  Yuji Takagi ,  Ryo Miyake ,  Kuniyasu Nakamura ,  Mitsugu Sato ,  Hiroyuki Kobayashi

发明人： Hiroshi Kakibayashi ,  Shigeyuki Hosoki ,  Yuji Takagi ,  Ryo Miyake ,  Kuniyasu Nakamura ,  Mitsugu Sato ,  Hiroyuki Kobayashi

IPC分类号： G01N23/04 ,  G01N1/28 ,  G01N23/225 ,  G01N33/483 ,  G01N37/00 ,  H01J37/20 ,  H01J37/22 ,  H01J37/26 ,  H01J37/28 ,  H01J49/44

CPC分类号： H01J37/22 ,  H01J37/26 ,  H01J2237/05 ,  H01J2237/2809 ,  H01J2237/2813

摘要： A bio electron microscope and an observation method which can observe a bio specimen by low damage and high contrast to perform high-accuracy image analysis, and conduct high-throughput specimen preparation. 1) A specimen is observed at an accelerating voltage 1.2 to 4.2 times a critical electron accelerating voltage possible to transmit a specimen obtained under predetermined conditions. 2) An electron energy filter of small and simplified construction is provided between the specimen and an electron detector for imaging by the electron beam in a specified energy region of the electron beams transmitting the specimen. 3) Similarity between an observed image such as virus or protein in the specimen and a reference image such as known virus or protein is subjected to quantitative analysis by image processing. 4) A preparation protocol of the bio specimen is made into a chip using an MEMS technique, which is then mounted on a specimen stage part of an electron microscope to conduct specimen introduction, preparation and transfer onto a specimen holder.

摘要翻译： 一种生物电子显微镜和观察方法，可以通过低损伤和高对比度观察生物样品，进行高精度图像分析，并进行高通量样品制备。 1）在可能传输在预定条件下获得的样品的临界电子加速电压的1.2至4.2倍的加速电压下观察样品。 2）在样品和电子检测器之间提供小而简化结构的电子能过滤器，用于通过电子束在传输样品的电子束的规定能量区域内的电子束进行成像。 3）通过图像处理对样本中的观察图像（例如病毒或蛋白质）与已知病毒或蛋白质等参考图像之间的相似性进行定量分析。 4）使用MEMS技术将生物样品的制备方案制成芯片，然后将其安装在电子显微镜的样品台部分上，以将样品引入，准备和转移到样品架上。

公开(公告)号：US20050004765A1

公开(公告)日：2005-01-06

申请号：US10859143

申请日：2004-06-03

申请人： Tetsuro Miyamoto ,  Shigenori Togashi ,  Ryo Miyake ,  Naoya Sasaki

发明人： Tetsuro Miyamoto ,  Shigenori Togashi ,  Ryo Miyake ,  Naoya Sasaki

IPC分类号： G01N33/48 ,  C12Q1/02 ,  G01N33/15 ,  G01N33/50 ,  G06F17/30 ,  G06F19/00 ,  G06F19/12

CPC分类号： G01N33/502 ,  G01N33/5008 ,  G01N33/5082

摘要： The object of the present invention is to estimate the effect of working substances on actual living organisms such as cells and tissues. The present invention provides a method for estimation of the effect of working substance which includes the following steps (a) and (b): (a) a step of contacting a working substance with a model cell to measure a cell activity value of the model cell, and (b) a step of estimating the effect of the working substance on the target living organism when the working substance is allowed to act on the target living organism, using the cell activity value measured at the step (a) as an input value.

摘要翻译： 本发明的目的是估计工作物质对细胞和组织等实际生物体的影响。 本发明提供一种估计工作物质影响的方法，其包括以下步骤（a）和（b）：（a）使工作物质与模型细胞接触以测量模型的细胞活性值的步骤 （b）当使用步骤（a）测量的细胞活性值作为输入时，估计工作物质对目标生物体的影响的步骤，当使所述工作物质作用于所述目标生物体时， 值。

公开(公告)号：US06599477B1

公开(公告)日：2003-07-29

申请号：US09136070

申请日：1998-08-19

申请人： Ryo Miyake ,  Akira Koide ,  Takeo Takagi ,  Takao Terayama ,  Yasushi Nomura ,  Hiroshi Mitsumaki

发明人： Ryo Miyake ,  Akira Koide ,  Takeo Takagi ,  Takao Terayama ,  Yasushi Nomura ,  Hiroshi Mitsumaki

IPC分类号： G01N3504

CPC分类号： G01N35/1002 ,  F04B43/043 ,  G01N35/025 ,  G01N35/1016 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114998 ,  Y10T436/119163 ,  Y10T436/2575

摘要： A chemically analyzing apparatus includes a plurality of reaction containers, a reaction container holder for holding the plurality of reaction containers and for supplying a sample and reagent at predetermined positions, a plurality of reagent containers for holding reagent adapted to be supplied into the reaction containers, a reagent container holder provided above the reaction container holder, for holding the plurality of reagent containers, a measuring part for measuring physical properties of the sample, a liquid feed mechanism provided in a lower part of each of the plurality of reagent containers, for supplying a predetermined quantity of reagent from each of the reagent container into the associated reaction containers by means of the liquid feed mechanism. With this arrangement, it is possible to provide a chemically analyzing apparatus incorporating a reagent supply mechanism which can prevent reagent from being uselessly consumed, which does not substantially require washing liquid, which does not require periodical disassembly and washing, and which can easily change the feed quantity of reagent.

摘要翻译： 化学分析装置包括多个反应容器，用于保持多个反应容器并用于在预定位置供应样品和试剂的反应容器保持器，用于保持适于供应到反应容器中的试剂的多个试剂容器， 设置在反应容器保持器上方的用于保持多个试剂容器的试剂容器保持器，用于测量样品的物理性质的测量部件，设置在多个试剂容器的每个的下部的液体供给机构，用于供给 通过液体供给机构将来自每个试剂容器的预定量的试剂输送到相关联的反应容器中。 通过这种布置，可以提供一种化学分析装置，其包括试剂供给机构，该试剂供给机构能够防止不需要定期拆卸和洗涤的基本上不需要洗涤液的试剂被无用地消耗，并且可以容易地改变 试剂的供料量。

公开(公告)号：US06398930B2

公开(公告)日：2002-06-04

申请号：US09828946

申请日：2001-04-10

申请人： Masao Fukunaga ,  Tamio Ishihara ,  Koji Saito ,  Katsutoshi Yamada ,  Hideo Enoki ,  Sadao Mori ,  Ryo Miyake ,  Takao Terayama ,  Masatoshi Kanamaru

发明人： Masao Fukunaga ,  Tamio Ishihara ,  Koji Saito ,  Katsutoshi Yamada ,  Hideo Enoki ,  Sadao Mori ,  Ryo Miyake ,  Takao Terayama ,  Masatoshi Kanamaru

IPC分类号： G01N2700

CPC分类号： C02F1/008 ,  C02F2103/02 ,  C02F2201/008 ,  C02F2201/009 ,  C02F2209/11 ,  C02F2209/29 ,  C02F2209/40 ,  G01N33/1893 ,  Y02A20/212

摘要： A water quality meter is composed of a plurality of analyzing units for analyzing water samples introduced from a water distribution pipe, each analyzing unit including a reagent mixing cell and a measuring cell, and a liquid introducing unit integrated with the analyzing units, which is composed of a single member in which a plurality of fluid flows paths for feeding various types of liquid including the water sample into the analyzing unit are formed. Furthermore, the cells and the plurality of three-dimensional fluid flow paths formed in the single member are fabricated by a micro-fabrication technique using photo-curing resin. In one embodiment, a photometer is situated in the flow path in at least one analyzing unit for measuring absorbency of the liquid. In another embodiment, a pair of electrodes, for measuring electric conductivity or pH, is disposed in a measurement flow path in at least one of the analyzing units.

摘要翻译： 水质计由多个用于分析从配水管引入的水样的分析单元构成，每个分析单元包括试剂混合室和测量池，以及与分析单元一体化的液体导入单元，其组成 其中形成有多个流体流动路径的单个构件，用于将包括水样本的各种类型的液体进料到分析单元中。 此外，通过使用光固化树脂的微制造技术来制造在单个构件中形成的电池和多个三维流体流动路径。 在一个实施例中，光度计位于至少一个分析单元中的流路中，用于测量液体的吸收性。 在另一个实施例中，用于测量电导率或pH的一对电极设置在至少一个分析单元中的测量流路中。

公开(公告)号：US5270212A

公开(公告)日：1993-12-14

申请号：US841487

申请日：1992-02-26

申请人： Hideyuki Horiuchi ,  Shinichi Sakuraba ,  Toshio Kaneko ,  Nobuyuki Tatara ,  Ryohei Yabe ,  Hiroshi Ohki ,  Isao Yamazaki ,  Ryo Miyake

发明人： Hideyuki Horiuchi ,  Shinichi Sakuraba ,  Toshio Kaneko ,  Nobuyuki Tatara ,  Ryohei Yabe ,  Hiroshi Ohki ,  Isao Yamazaki ,  Ryo Miyake

IPC分类号： G01N1/00 ,  G01N1/28 ,  G01N15/00 ,  G01N15/14 ,  G01N33/48 ,  G01N35/00

CPC分类号： G01N35/1095 ,  G01N1/28 ,  G01N2015/0069 ,  G01N2015/0076 ,  G01N2015/1409 ,  G01N2021/6439 ,  Y10T436/111666

摘要： This invention relates to an apparatus for analyzing cells in a biological liquid sample, such as blood, in which apparatus a flow passage in a sample pretreatment system can be shortened with a simple construction. A sample metering chamber, a mixing chamber and a measurement liquid extracting chamber are formed in a rotary sliding member of a pretreatment portion. The rotary sliding member is intermittently rotated so as to shift a sample from one chamber to another to perform a necessary treatment. Fluorescence or scattering light with respect to the sample, mixed with a reagent in the pretreatment portion, is detected by a flow cell.

摘要翻译： 本发明涉及用于分析生物液体样品如血液中的细胞的装置，其中可以用简单的结构缩短样品预处理系统中的流动通道。 样品计量室，混合室和测量液体提取室形成在预处理部分的旋转滑动构件中。 旋转滑动构件间歇地旋转，以将样品从一个室移动到另一个室进行必要的处理。 通过流动池检测相对于样品的荧光或散射光与预处理部分中的试剂混合。

公开(公告)号：US5174162A

公开(公告)日：1992-12-29

申请号：US550543

申请日：1990-07-10

申请人： Ryo Miyake ,  Hiroshi Ohki ,  Hideo Enoki ,  Toshio Kaneko ,  Hideyuki Horiuchi

发明人： Ryo Miyake ,  Hiroshi Ohki ,  Hideo Enoki ,  Toshio Kaneko ,  Hideyuki Horiuchi

IPC分类号： B01F5/06 ,  B01F11/00 ,  B01F13/00 ,  B01L3/02 ,  G01N1/00 ,  G01N21/78 ,  G01N33/543 ,  G01N35/02 ,  G01N35/10

CPC分类号： B01F11/0071 ,  B01F11/0074 ,  B01F13/00 ,  B01F5/0609 ,  B01L3/021 ,  G01N35/10 ,  G01N2035/1058

摘要： A pipetter capable of mixing liquids and dispensing the mixed liquid has a pipetting tube, an actuating device connected to the tube for sucking liquid into and discharging liquid from the tube, and a flow disturbance device in the tube spaced from the discharge and suction end thereof and adapted to cause longitudinal mixing of liquid in the tube when liquid is moved along the tube in at least one direction. Mixing is effected in the tube by causing a liquid body to move repeatedly to and fro along the tube so that during each such movement at least a portion of the liquid body passes the flow disturbance device and the liquid body is not discharged from the first end of the tube. The pipetter is useful for example in mixing a suspension of blood cells with a reagent prior to analysis.

摘要翻译： 能够混合液体并分配混合液体的移液器具有移液管，连接到管的致动装置，用于将液体从管吸入和排出液体，以及与排出和吸入端隔开的管中的流动扰动装置 并且适于当液体沿着至少一个方向移动时引起液体在管中的纵向混合。 通过使液体沿着管道来回重复地在管中进行混合，使得在每次这样的移动期间，液体的至少一部分通过流动扰动装置，液体不从第一端排出 的管。 移液器可用于例如在分析之前将血细胞悬浮液与试剂混合。

公开(公告)号：US5007732A

公开(公告)日：1991-04-16

申请号：US182430

申请日：1988-04-18

申请人： Hiroshi Ohki ,  Hideaki Kamohara ,  Ryo Miyake

发明人： Hiroshi Ohki ,  Hideaki Kamohara ,  Ryo Miyake

IPC分类号： G01N33/483 ,  C12M1/34 ,  G01N15/14

CPC分类号： G01N15/1404

摘要： A sheath flow type flow-cell device for plow-cytometer which comprises a first inlet for sheath fluid, a flow passage communicated with the first inlet and contracted toward downstream, the flow passage having a substantially rectangular cross section, a straight capillary flow passage connected to the flow passage downstream thereof, the capillary flow passage having a substantially rectangular cross section, a second inlet for sample fluid, a nozzle communicating with the second inlet and opened within the flow passage in the same direction as the flow direction of the straight capillary flow passage, a discharge port provided at a terminal end of the straight capillary flow passage, and flow regulating means for regulating the flow of the sheath fluid in the straight capillary flow passage to be a laminar flow having a gradient of flow velocity.