公开(公告)号：US20140166562A1

公开(公告)日：2014-06-19

申请号：US14238278

申请日：2012-08-23

Applicant: Joseph D. Michienzi ,  Keith Fadgen ,  Wade P. Leveille, Sr. ,  Raymond P. Fisk ,  Frank John Marzalkowski, Jr.

Inventor： Joseph D. Michienzi ,  Keith Fadgen ,  Wade P. Leveille, Sr. ,  Raymond P. Fisk ,  Frank John Marzalkowski, Jr.

IPC: B01D15/22

CPC classification number: B05B5/16 ,  B01D15/10 ,  B01D15/1871 ,  B01D15/22 ,  G01N30/60 ,  G01N30/6004 ,  G01N30/6026 ,  G01N30/603 ,  G01N30/6039 ,  G01N30/6052 ,  G01N30/6095 ,  G01N30/7266 ,  Y10T29/49908

Abstract: Described is a chromatography column assembly that includes a permanently deformable outer tube, an intermediate tube, an inner tube and a sorbent bed disposed within the inner tube. The sorbent bed may be in the form of packed chromatographic particles or a porous monolithic structure. A radial seal is provided by one or more uniform radial crimps at longitudinal locations on the assembly. The uniform radial crimp compresses the outer tube and underlying intermediate tube onto the inner tube to achieve a high pressure liquid tight seal between the three tubes. The length and depth of each crimp is accurately formed to accommodate the requirements of the particular application. Leakage along the tubing assembly is prevented and void volume is reduced or eliminated. No external ferrule or ferrule swaging mechanism is needed; therefore the chromatography column assembly is easily adapted for use in various chromatographic column configurations.

Abstract translation: 描述了一种色谱柱组件，其包括永久变形的外管，中间管，内管和设置在内管内的吸附剂床。 吸附剂床可以是填充色谱颗粒或多孔整体结构的形式。 在组件的纵向位置处由一个或多个均匀的径向卷曲提供径向密封。 均匀的径向卷曲将外管和下面的中间管压缩到内管上，以实现三管之间的高压液密封。 精确地形成每个卷曲的长度和深度以适应特定应用的要求。 防止管道组件的泄漏，减少或消除空隙体积。 不需要外部套圈或套圈型锻机构; 因此色谱柱组件易于适用于各种色谱柱配置。

公开(公告)号：US08679332B2

公开(公告)日：2014-03-25

申请号：US11959602

申请日：2007-12-19

Applicant: Geoff C. Gerhardt ,  Keith Fadgen

Inventor： Geoff C. Gerhardt ,  Keith Fadgen

IPC: B01D15/08

CPC classification number: G01F1/363 ,  G01F1/68 ,  G01N30/10 ,  G01N30/30 ,  G01N30/32 ,  G01N2030/324 ,  G01N2030/326

Abstract: A method and apparatus for monitoring and controlling the nano-scale flow rate of fluid in the operating flow path of a HPLC system without relying on a nano-scale sensor in the operating flow path. A main flow sensor is disposed in the main flow path between the pump and a flow-divider. A waste flow sensor is disposed in the waste flow path downstream of the splitter. The output signal of the waste flow sensor is subtracted from the output signal of the main flow sensor in a difference circuit. The difference signal is divided by the output signal from the main flow sensor in a divider circuit. The output of the divider circuit represents an empirical split ratio of the flow-divider and is independent of media composition.

Abstract translation: 一种用于在不依赖于操作流程中的纳米级传感器的情况下监视和控制HPLC系统的操作流路中的流体的纳米流量的方法和装置。 主流量传感器设置在泵和分流器之间的主流路中。 废液流量传感器设置在分流器下游的废流路中。 在差分电路中，从主流量传感器的输出信号中减去废液流量传感器的输出信号。 差分信号除以分流器电路中的主流量传感器的输出信号。 分压电路的输出表示分流器的经验分流比，并且与介质组成无关。

公开(公告)号：US20120171773A1

公开(公告)日：2012-07-05

申请号：US13321666

申请日：2010-05-27

Applicant: James P. Murphy ,  Keith Fadgen ,  Geoff C. Gerhardt ,  Angela Doneanu ,  Martha Degen Stapels

Inventor： James P. Murphy ,  Keith Fadgen ,  Geoff C. Gerhardt ,  Angela Doneanu ,  Martha Degen Stapels

IPC: G01N30/02

CPC classification number: G01N30/6095 ,  B01D15/22 ,  B01D15/424 ,  B01L3/502715 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2300/0816 ,  B01L2400/0487 ,  G01N30/461 ,  G01N30/606 ,  G01N30/6091 ,  G01N30/7233 ,  G01N30/7266 ,  G01N2030/027 ,  G01N2030/085 ,  G01N2030/8881 ,  Y10T29/49826

Abstract: A method of analyzing samples includes loading a sufficient quantity of the sample onto a trap column to overload the trap column; heating an analytical column and the trap column to a greater temperature than the analytical column; and pumping a solvent, to the trap column, having a solvent composition profile that, in cooperation with a temperature differential, causes at least some of the components to elute sequentially from the trap column to the analytical column and focus on the analytical column prior to eluting from the analytical column; or optionally: loading a small-molecule sample onto a cooled portion of an analytical column; heating the analytical column; and pumping a solvent, to the heated analytical column, to elute the components from the analytical column. Chromatographic separation includes: a trap column; a separation column; a trap-column heater; a separation-column heater; a solvent pump unit; and a control unit can be used.

Abstract translation: 分析样品的方法包括将足够量的样品加载到陷阱柱上以使捕获柱过载; 将分析柱和捕集柱加热至比分析柱更大的温度; 并将溶剂泵送到捕集塔，其具有溶剂组成分布，其与温差相配合使至少一些组分从捕集塔顺序洗脱至分析柱，并在分离柱之前聚焦在分析柱上 从分析柱洗脱; 或任选地：将小分子样品加载到分析柱的冷却部分上; 加热分析柱; 并将溶剂泵入加热的分析柱，以从分析柱中洗脱组分。 色谱分离包括：陷阱柱; 分离柱; 一个陷阱加热器; 分离柱加热器; 溶剂泵单元; 并且可以使用控制单元。

公开(公告)号：US20110272855A1

公开(公告)日：2011-11-10

申请号：US13125182

申请日：2009-10-28

Applicant: Joseph A. Luongo ,  Michael Budnick ,  Mark W. Moeller ,  James P. Murphy ,  Geoff C. Gerhardt ,  Keith Fadgen ,  Joseph D. Michienzi

Inventor： Joseph A. Luongo ,  Michael Budnick ,  Mark W. Moeller ,  James P. Murphy ,  Geoff C. Gerhardt ,  Keith Fadgen ,  Joseph D. Michienzi

IPC: B29C59/02

CPC classification number: F16K11/074 ,  F16K25/005 ,  G01N2030/202

Abstract: Described are techniques for fabricating one or more parts of a valve used in a liquid chromatography system. At least one of a rotor and a stator are provided. The rotor is included in the valve and has a first surface facing a stator. The stator is included in the valve and has a second surface facing the rotor. A pattern is formed in at least one of the first surface and the second surface. Forming the pattern includes compressing the at least one surface by applying pressure thereto causing displacement of material to form at least one groove.

Abstract translation: 描述了用于制造用于液相色谱系统的阀的一个或多个部分的技术。 提供转子和定子中的至少一个。 转子包括在阀中并且具有面向定子的第一表面。 定子包括在阀中并且具有面向转子的第二表面。 在第一表面和第二表面中的至少一个中形成图案。 形成图案包括通过施加压力来压缩至少一个表面，引起材料的移位以形成至少一个凹槽。

公开(公告)号：US20100043539A1

公开(公告)日：2010-02-25

申请号：US12282895

申请日：2007-03-16

Applicant: Keith Fadgen ,  James E. Usowicz ,  Miguel Soares

Inventor： Keith Fadgen ,  James E. Usowicz ,  Miguel Soares

IPC: G01N30/02

CPC classification number: G01N30/20 ,  G01N30/22 ,  G01N2030/201 ,  G01N2030/202 ,  G05D7/0688 ,  G05D16/208

Abstract: Embodiments of the present invention are directed to methods and apparatus for placing a sample in a chromatographic system. The device and method feature placing samples held in a sample loop to pressurization prior to placing such sample loop in communication with high pressure conduits.

Abstract translation: 本发明的实施方案涉及将样品置于色谱系统中的方法和装置。 该装置和方法的特征是将放置样品回路中的样品置于加压之前，使样品环与高压导管连通。

公开(公告)号：US20090205409A1

公开(公告)日：2009-08-20

申请号：US12280585

申请日：2007-03-16

Applicant: Steven J. Ciavarini ,  Stanley P. Pensak, JR. ,  Jeffrey W. Finch ,  Keith Fadgen ,  Hongji Liu

Inventor： Steven J. Ciavarini ,  Stanley P. Pensak, JR. ,  Jeffrey W. Finch ,  Keith Fadgen ,  Hongji Liu

IPC: G01N30/02

CPC classification number: G01N30/34 ,  G01N30/32 ,  G01N2030/326 ,  G05D11/132

Abstract: A solvent delivery subsystem for a chromatography device performs relatively low pressure, high flow mixing of solvents to form a gradient and subsequent high pressure, low flow delivery of the gradient to the separation column. The mixing of the gradient is independent and does not interfere with the gradient delivery. To form the gradient, the outputs of an aqueous pump and an organic pump are mixed to fill a storage capillary while a downstream point from the storage capillary is vented to atmosphere. After gradient formation, the vent to atmosphere is closed, the solvent delivery system rises to high pressure, and only the aqueous pump runs for gradient delivery. To maintain integrity of the fluid stream, the solvent delivery system uses feed forward compensation and controls at least one parameter selected from the group consisting of pressure and flow in the conduit means to follow a gradual ramp.

Abstract translation: 用于色谱装置的溶剂递送子系统执行溶剂的相对低压，高流动混合以形成梯度并随后将高梯度梯度递送到分离塔。 梯度的混合是独立的，并且不干扰梯度递送。 为了形成梯度，将水性泵和有机泵的输出混合以填充储存毛细管，同时从储存毛细管的下游点排放到大气中。 梯度形成后，关闭空气，溶剂输送系统升高至高压，只有水泵运行梯度输送。 为了保持流体流的完整性，溶剂输送系统使用前馈补偿并且控制选自管道装置中的压力和流量的至少一个参数以跟随逐渐斜坡。

公开(公告)号：US07332087B2

公开(公告)日：2008-02-19

申请号：US10723973

申请日：2003-11-26

Applicant: Geoff C. Gerhardt ,  Keith Fadgen

Inventor： Geoff C. Gerhardt ,  Keith Fadgen

IPC: B01D15/08

CPC classification number: G01F1/363 ,  G01F1/68 ,  G01N30/10 ,  G01N30/30 ,  G01N30/32 ,  G01N2030/324 ,  G01N2030/326

Abstract: A method and apparatus for monitoring and controlling the nano-scale flow rate of fluid in the operating flow path of a HPLC system without relying on a nano-scale sensor in the operating flow path. A main flow sensor is disposed in the main flow path between the pump and a flow-divider. A waste flow sensor is disposed in the waste flow path downstream of the splitter. The output signal of the waste flow sensor is subtracted from the output signal of the main flow sensor in a difference circuit. The difference signal is divided by the output signal from the main flow sensor in a divider circuit. The output of the divider circuit represents an empirical split ratio of the flow-divider and is independent of media composition.

Abstract translation: 一种用于监测和控制HPLC系统的操作流路中的流体的纳米级流速的方法和装置，而不依赖于操作流路中的纳米级传感器。 主流量传感器设置在泵和分流器之间的主流路中。 废液流量传感器设置在分流器下游的废流路中。 在差分电路中，从主流量传感器的输出信号中减去废液流量传感器的输出信号。 差分信号除以分流器电路中的主流量传感器的输出信号。 分压电路的输出表示分流器的经验分流比，并且与介质组成无关。