公开(公告)号：US20130217584A1

公开(公告)日：2013-08-22

申请号：US13814213

申请日：2010-08-06

申请人： Guanbin Zhang ,  Guangxin Xiang ,  Wanli Xing ,  Jing Cheng

发明人： Guanbin Zhang ,  Guangxin Xiang ,  Wanli Xing ,  Jing Cheng

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6837 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2563/155

摘要： A microarray-based assay is provided, which is used for analyzing molecular interactions, including polynucleotides, polypeptides, antibodies, small molecule compounds, peptides and carbohydrates. Such method comprises coupling a target molecule to a particle and then binding to a probe molecule on microarray. In particular, multiplexed genetic analysis of nucleic acid fragments can be implemented. Specific genes, single nucleotide polymorphisms or gene mutations, such as deletions, insertions, and indels, can be identified. Coupled with microarray, the particles, themselves or further modified, facilitate the detection of results with non-expensive devices or even naked eyes. This technology enables the detection and interpretation of molecular interactions in an efficient and cost effective way.

摘要翻译： 提供基于微阵列的测定法，其用于分析分子相互作用，包括多核苷酸，多肽，抗体，小分子化合物，肽和碳水化合物。 这种方法包括将靶分子偶联到颗粒上，然后与微阵列上的探针分子结合。 特别地，可以实现核酸片段的多重遗传分析。 可以鉴定特异性基因，单核苷酸多态性或基因突变，例如缺失，插入和插入。 与微阵列结合，颗粒本身或进一步修饰，有助于使用非昂贵装置甚至肉眼检测结果。 该技术能够以有效和成本有效的方式检测和解释分子相互作用。

公开(公告)号：US20130206250A1

公开(公告)日：2013-08-15

申请号：US13805323

申请日：2011-07-01

申请人： Guohao Zhang ,  Su Guo ,  Can Wang ,  Guoqing Wang ,  Miao Liu ,  Jinxiu Zhang ,  Bei Han ,  Tao Deng ,  Wanli Xing ,  Jing Cheng

发明人： Guohao Zhang ,  Su Guo ,  Can Wang ,  Guoqing Wang ,  Miao Liu ,  Jinxiu Zhang ,  Bei Han ,  Tao Deng ,  Wanli Xing ,  Jing Cheng

IPC分类号： F04B43/00

CPC分类号： F04B43/00 ,  B01L3/502738 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/16 ,  B01L2300/1827 ,  B01L2400/0633 ,  B01L2400/0638 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0019 ,  F16K99/0028 ,  F16K99/0034 ,  F16K99/0044 ,  F16K2099/0084 ,  Y10T137/4238

摘要： Provided are a bubble-based microvalve and a microfluidic chip using the microvalve. Also provided are methods of using the microvalve for manipulating fluid in a microfluidic channel by changing the volume and/or location of the gas in the microvalve.

摘要翻译： 提供了基于气泡的微型阀和使用微型阀的微流控芯片。 还提供了通过改变微型阀中的气体的体积和/或位置来使用微型阀来在微流体通道中操纵流体的方法。

公开(公告)号：US07939035B2

公开(公告)日：2011-05-10

申请号：US11661019

申请日：2004-11-22

申请人： Renyuan Chen ,  Guoqing Wang ,  Feijun Xian ,  Wanli Xing ,  Xianhua Wang ,  Jing Cheng

发明人： Renyuan Chen ,  Guoqing Wang ,  Feijun Xian ,  Wanli Xing ,  Xianhua Wang ,  Jing Cheng

IPC分类号： B01D21/00 ,  B01L99/00

CPC分类号： G01N1/40 ,  G01N2001/2866

摘要： The present invention provides a sample preparation device for extracting drug residue. It comprises a case, in which a water bath, a vortex mixer, and a homogenizer are integrated. The case also has on it an integrated control panel for controlling heating temperature of the water bath and homogenization time of the homogenizer.

摘要翻译： 本发明提供了用于提取药物残留物的样品制备装置。 它包括其中集成了水浴，涡流混合器和均化器的壳体。 该情况还具有用于控制水浴的加热温度和均化器的均化时间的集成控制面板。

公开(公告)号：US07767438B2

公开(公告)日：2010-08-03

申请号：US10546226

申请日：2003-03-28

申请人： Wanli Xing ,  Fei Jun Xian ,  Xianhua Wang ,  Jing Cheng

发明人： Wanli Xing ,  Fei Jun Xian ,  Xianhua Wang ,  Jing Cheng

IPC分类号： C12M1/34 ,  C12M3/00

CPC分类号： C40B30/04 ,  B01J2219/00509 ,  B01J2219/00542 ,  B01J2219/00574 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B01L3/50853 ,  B01L2300/0636 ,  B01L2300/0809 ,  B01L2300/0822 ,  B01L2400/0406 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12

摘要： This invention relates generally to the field of microarray reaction devices and uses thereof. In particular, the invention provides a microarray reaction device wherein a plurality of reaction spaces are formed between a first and second plurality of projections, the heights of said plurality of reaction spaces being substantially identical and controllable by a supporting structure, and the relative positions between the first and second plurality of projections being controllable by a positioning structure. Articles of manufacture comprising the microarray reaction device and methods for assaying an analyte using the microarray reaction device are also provided.

摘要翻译： 本发明一般涉及微阵列反应装置的领域及其用途。 特别地，本发明提供一种微阵列反应装置，其中在第一和第二多个突起之间形成多个反应空间，所述多个反应空间的高度基本上相同并且可由支撑结构控制， 所述第一和第二多个突起可由定位结构控制。 还提供了包含微阵列反应装置的制品和使用微阵列反应装置测定分析物的方法。

公开(公告)号：US20090137411A1

公开(公告)日：2009-05-28

申请号：US10593908

申请日：2005-03-28

申请人： Yimin Sun ,  Wanli Xing ,  Guoqing Wang ,  Hongwu Du ,  Rong Zhang ,  Yuan Lu ,  Yan Wang ,  Jing Cheng

发明人： Yimin Sun ,  Wanli Xing ,  Guoqing Wang ,  Hongwu Du ,  Rong Zhang ,  Yuan Lu ,  Yan Wang ,  Jing Cheng

IPC分类号： C40B30/04 ,  C40B40/04 ,  C40B50/18

CPC分类号： G01N33/543 ,  B01J2219/00387 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/0072 ,  C40B60/14 ,  G01N33/54366

摘要： The present invention discloses methods for detection of small molecule compounds and its specific biochips. Biochips of the present invention comprise a solid support and carrier-linked small molecules immobilized onto the solid support. The invention also provides methods and kits for detection of small molecule compounds using the biochips of the invention.

摘要翻译： 本发明公开了小分子化合物及其特定生物芯片的检测方法。 本发明的生物芯片包含固体支持物和固定在固体支持物上的载体连接的小分子。 本发明还提供了使用本发明的生物芯片检测小分子化合物的方法和试剂盒。

公开(公告)号：US07527719B2

公开(公告)日：2009-05-05

申请号：US10500180

申请日：2002-11-29

申请人： Peng Liu ,  Wanli Xing ,  Dong Liang ,  Jing Cheng

发明人： Peng Liu ,  Wanli Xing ,  Dong Liang ,  Jing Cheng

IPC分类号： G01N27/453

CPC分类号： G01N27/44791

摘要： The present invention provides a capillary electrophoresis chip apparatus for detecting nucleotide polymorphism or single nucleotide polymorphism belonging to capillary electrophoresis apparatuses. The apparatus comprises an upper channel layer comprising a one-, two-, or multi-dimensional microfluid channel and an electrode aperture structure for loading sample, a middle electrode layer for sealing the microfluid channel to form an intact capillary and providing the needed voltage for electrophoresis; and a lower heating layer for providing a stable temperature gradient for electrophoresis. The upper, middle and lower layers are thermal conductive and adhesive to each other.

摘要翻译： 本发明提供一种用于检测属于毛细管电泳装置的核苷酸多态性或单核苷酸多态性的毛细管电泳芯片装置。 该装置包括上通道层，其包括一维，二维或多维微流体通道和用于装载样品的电极孔结构，用于密封微流体通道以形成完整毛细管的中间电极层，并提供所需电压 电泳; 以及用于提供电泳的稳定温度梯度的较低加热层。 上，中，下层相互导热，粘合。

公开(公告)号：US20060144237A1

公开(公告)日：2006-07-06

申请号：US10537533

申请日：2002-12-31

申请人： Dong Liang ,  Wanli Xing ,  Jing Cheng

发明人： Dong Liang ,  Wanli Xing ,  Jing Cheng

IPC分类号： B01D53/02 ,  B01D53/14

CPC分类号： G01N30/6095 ,  G01N30/6039 ,  G01N2030/025

摘要： This invention relates to the field of miniaturing gas chromatograph instruments using microfabrication technologies. In particular, the invention provides a gas chromatograph column, which column comprises at least two lid layers and a channel layer, wherein each of said layers comprises a compact material suitable for gas chromatograph, said channel layer comprises microfabricated channels on both sides, said microfabricated channels and a side of said lid layers form at least two capillaries, said at least two capillaries are connected to each other through a hole in said channel layer to form an integrated capillary, said integrated capillary is connected to outside atmosphere on both ends via holes on two outmost lid layers to serve as an inlet and an outlet.

摘要翻译： 本发明涉及使用微细加工技术的微型气相色谱仪的领域。 特别地，本发明提供了一种气相色谱柱，该色谱柱包括至少两个盖层和沟道层，其中每个所述层包括适合于气相色谱的致密材料，所述通道层包括两侧的微细加工通道，所述微制造 通道和所述盖层的一侧形成至少两个毛细管，所述至少两个毛细管通过所述通道层中的孔彼此连接以形成集成毛细管，所述集成毛细管连接到两端的外部大气通孔 在两个最外层的盖层上用作入口和出口。

公开(公告)号：US20050106708A1

公开(公告)日：2005-05-19

申请号：US10499098

申请日：2002-11-29

申请人： Wanli Xing ,  Zhe Yu ,  Guangxin Xiang ,  Liangbin Pan ,  Jing Cheng

发明人： Wanli Xing ,  Zhe Yu ,  Guangxin Xiang ,  Liangbin Pan ,  Jing Cheng

IPC分类号： C12M1/42 ,  C12M1/34 ,  C12N13/00 ,  C12Q1/02 ,  G01N33/543 ,  G01F1/64

CPC分类号： G01N33/5438 ,  C12M35/02 ,  G01N2333/46 ,  G01N2500/10

摘要： The present invention provides an apparatus for stimulating an animal cell and recording its physiological signal and methods of making and using thereof. The purpose of the present invention is to provide an apparatus for stimulating an animal cell and recording its physiological signal that is efficient, convenient, and accurate. The apparatus for stimulating an animal cell and recording its physiological signal of the present invention comprises a poor conductive substrate, wherein on at least one surface of the substrate is provided at least one unit conductive polymer layer and at least one good conductive microelectrode.

摘要翻译： 本发明提供一种用于刺激动物细胞并记录其生理信号的装置及其制造和使用方法。 本发明的目的是提供一种用于刺激动物细胞并记录其生理信号的装置，其有效，方便和准确。 用于刺激动物细胞并记录其本发明的生理信号的装置包括差的导电基底，其中在基底的至少一个表面上提供至少一个单位导电聚合物层和至少一个良好的导电微电极。

公开(公告)号：US09671317B2

公开(公告)日：2017-06-06

申请号：US14006488

申请日：2012-02-10

申请人： Jiaming Ye ,  Pinhong Wang ,  Guoqing Wang ,  Wanli Xing

发明人： Jiaming Ye ,  Pinhong Wang ,  Guoqing Wang ,  Wanli Xing

IPC分类号： G01N1/10 ,  G01N35/10 ,  C12Q1/68 ,  G01N33/543 ,  G01N33/68 ,  C40B30/04 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12

CPC分类号： G01N1/10 ,  C12Q1/6813 ,  C40B30/04 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  G01N33/543 ,  G01N33/68 ,  G01N35/1095

摘要： An automatic injection device comprises at least an injection unit (1). The said injection unit (1) is formed by sealing a cover plate layer (3) with hydrophilic surfaces and a microfluid layer (4). The said cover plate layer (3) is provided with at least two through holes (5). The said microfluid layer (4) is provided with a hollow-out hybridization chamber (7) and at least two hollow-out microfluid channels (6). One end of each channel (6) is connected with the hybridization chamber (7), and the other end is connected with a through hole (5) of the cover plate layer (3) respectively. Taking advantage of the hydrophilicity of the cover plate, the automatic injection device makes a solution automatically enter and fill the hybridization chamber (7) and the microfluid channels (6) by the driving force of liquid surface tension. The flow uniformity of sample solution in microarray chip is achieved by the structural design of the hybridization chamber (7) and the microfluid channels (6). The automatic injection device has advantages of simple manufacture, easy operation, high hybridization efficiency, low sample cost, and automatic quantificational injection.

公开(公告)号：US09677133B2

公开(公告)日：2017-06-13

申请号：US14006540

申请日：2012-02-10

申请人： Jiaming Ye ,  Pinhong Wang ,  Guoqing Wang ,  Liqing Duan ,  Kaijun Zhao ,  Jinxiu Zhang ,  Xiumei Song ,  Dong Wang ,  Huafang Gao ,  Wanli Xing

发明人： Jiaming Ye ,  Pinhong Wang ,  Guoqing Wang ,  Liqing Duan ,  Kaijun Zhao ,  Jinxiu Zhang ,  Xiumei Song ,  Dong Wang ,  Huafang Gao ,  Wanli Xing

IPC分类号： C12Q1/68 ,  G01N33/50 ,  G01N33/543 ,  B01L3/00 ,  B01L7/00 ,  G01N35/00

CPC分类号： C12Q1/6876 ,  B01L3/5027 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2400/0622 ,  G01N33/543 ,  G01N2035/00158

摘要： A hybridization system is provided, which comprises a biological chip having a substrate (1) with a probe dot matrix (3) and a cover plate (2) with at least two through-holes (4), where a hybrid chamber (5) is formed between the substrate (1) and the cover plate (2), at least two fluid channels (6) are interconnected respectively with the hybrid chamber (5) by the two through-holes (4), and a fluid control device is interconnected with the fluid channels (6). The biological chip hybridization system integrates hybridizing, cleaning and drying functions, uses power provided by the fluid control device as the drive force for promoting the liquid flow for automatically reciprocation flow in the fluid channels (6) and the chamber (5) to achieve a dynamic hybridization, thus improving the hybrid efficiency and uniformity, and achieving automatic control.